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Characterization of Pectin Nanocoatings at Polystyrene and Titanium Surfaces

Journal of Surface Engineered Materials and Advanced Technology 2013年第4期3卷 20-28页

作者： Katarzyna Gurzawska Kai Dirscherl Yu Yihua Inge Byg Bodil jorgensen rikke Svava Martin W.Nielsen niklas r.jorgensen Klaus Gotfredsen research Center for Ageing and Osteoporosis Departments of Medicine and DiagnosticsCopenhagen University Hospital GlostrupGlostrupDenmark Institute of Odontology Faculty of Health and Medical SciencesUniversity of CopenhagenCopenhagenDenmark Dansk Fundamental Metrologi A/S LyngbyDenmark Microtechnology and Surface Analysis Danish Technological InstituteTaastrupDenmark Department of Plant and Environment Sciences Faculty of ScienceUniversity of CopenhagenFrederiksbergDenmark Department of Systems Biology Technical University of DenmarkLyngbyDenmark

The titanium implant surface plays a crucial role for implant incorporation into bone. A new strategy to improve implant integration in a bone is to develop surface nanocoatings with plant-derived polysaccharides able to increase adhesion of bone cells to the implant surface. The aim of the present study was to physically characterize and compare polystyrene and titanium surfaces nanocoated with different rhamnogalacturonan-Is (rG-I) and to visualize rG-I nanocoatings. rG-Is from potato and apple were coated on aminated surfaces of polystyrene, titianium discs and titanium implants. To characterize, compare and visualize the surface nanocoatings measurements of contact angle measurements and surface roughness with atomic force microscopy, scanning electron microscopy, and confocal microscopy was performed. We found that, both unmodified and enzymatic modified rG-Is influenced surface wettability, without any major effect on surface roughness (Sa, Sdr). Furthermore, we demonstrated that it is possible to visualize the pectin rG-Is molecules and even the nanocoatings on titanium surfaces, which have not been presented before. The comparison between polystyrene and titanium surface showed that the used material affected the physical properties of non-coated and coated surfaces. rG-Is should be considered as a candidate for new materials as organic nanocoatings for biomaterials in order to improve bone healing.

关键词： Surface Properties Titanium Polystyrene rhamnogalacturonan-I Osseointegration

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Bone mineral density in lifelong trained male football players compared with young and elderly untrained men

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Journal of Sport and Health Science 2018年第2期7卷 159-168页

作者： Marie Hagman Eva Wulff Helge Therese Hornstrup Bjorn Fristrup Jens Jung Nielsen niklas rye jorgensen Jesper Lovind Andersen Jorn Wulff Helge Peter Krustrup Department of Sports Science and Clinical Biomechanics SDU Sport and Health Sciences Cluster (SHSC) University of Southern Denmark Odense 3450 Denmark Department of Nutrition Exercise and Sports University of Copenhagen Copenhagen N 2200 Denmark Institute of Sports Medicine Copenhagen Bispebjerg Hospital Copenhagen NV 2400 Denmark Department of Clinical Biochemistry Rigshospitalet Research Center for Ageing and Osteoporosis Glostrup 2600 Denmark Center of Healthy Aging Department of Biomedical Sciences University of Copenhagen Copenhagen N 2200 Denmark Sport and Health Sciences University of Exeter Exeter EX1 2LU UK

Purpose: The purpose of the present controlled cross-sectional study was to investigate proximal femur and whole-body bone mineral density(BMD), as well as bone turnover profile, in lifelong trained elderly male football players and young elite football players compared with untrained age-matched ***: One hundred and forty healthy, non-smoking men participated in the study, including lifelong trained football players(FTE, n = 35)aged 65—80 years, elite football players(FTY, n = 35) aged 18—30 years, as well as untrained age-matched elderly(UE, n = 35) and young(UY,n = 35) men. All participants underwent a regional dual-energy X-ray Absorptiometry(DXA) scan of the proximal femur and a whole-body DXA scan to determine BMD. From a resting blood sample, the bone turnover markers(BTMs) osteocalcin, carboxy-terminal type-1 collagen crosslinks(CTX-1), procollagen type-1 amino-terminal propeptide(P1NP), and sclerostin were ***: FTE had 7.3%—12.9% higher(p ral neck, wards, shaft, and total proximal femur in both legs compared to UE,and 9.3%—9.7% higher(p ral trochanter in both legs compared to UY. FTY had 24.3%—37.4% higher(p ral regions and total proximal femur in both legs compared to UY. The whole-body DXA scan confirmed these results, with FTE showing similar whole-body BMD and 7.9% higher(p red to UY, and with FTY having 9.6% higher(p r(p red to UY. The plasma concentration of osteocalcin, CTX-1, and P1NP were 29%,53%, and 52% higher(p respectively, in FTY compared to ***: BMD of the proximal femur and whole-body BMD are markedly higher in lifelong trained male football players aged 65—80 years and young elite football players aged 18—30 years compared to age-matched untrained men. Elderly football players even show higher BMD in femoral trochanter and leg BMD than untrained young despi

关键词： Bone mass Bone turnover markers Dual-energy X-ray absorptiometry Proximal femur bone mineral density Soccer Whole-body bone mineral density

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