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Comparison of Different Immunologic Assays for Quantification of Human IL21 Production

Comparison of Different Immunologic Assays for Quantificatio...

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第八届全国免疫学学术大会

作者： Jenny Huang Theresa Bengtsson niklas ahlborg Mabtech AB SE-131 28 Stockholm Nacka Strand SwedenPh.D

IL-21 is primarily secreted by activated T cells, in particular CD4+ follicular and Th17 T cells, and regulates the development of T cells, B cells, NK cells and dendritic *** of IL-21 has been reported to contribute to diseases such as SLE and *** fundamental relevance of IL-21 as well as its involvement in inflammatory and autoimmune diseases highlights the need for development and evaluation of suitable immunoassays facilitating analysis of *** antibodies to human IL-21 were generated and used to develop sensitive capture ELISA and ELISpot *** assays were used to analyze IL-21 secretion by peripheral blood mononuclear cells(PBMC) after polyclonal(PHA) or specific stimulation(Tetanus toxoid;Candida albicans extract).Polyclonal activation of PBMC induced IL-21 production detectable by ELISpot and *** PBMC concentrations and pro-longed incubation times increased the number of IL-21-secreting cells found by ***-21 levels in supernatants, analyzed by ELISA, showed a reversed relationship with increasing cell concentrations and incubation times associated with a decrease in *** PBMC activated with specific stimuli, IL-21-producing cells could be detected by ELISpot whereas IL-21 levels in supernatants were not measurable by *** parallel, IL-17 production after specific stimuli, on the other hand, was detectable both by ELISpot and *** to enable detection of antigen-specific IL-21 responses by ELISA were *** were cultured in the presence of a m Ab blocking IL-21 from binding its *** that, antigen-specific IL-21 could be measured by *** results show that although IL-21 secretion is specifically induced by antigen, as seen in ELISpot, the secreted IL-21 is below detection limits and/or consumed by binding to ***, by preventing secreted IL-21 from binding to its receptor, antigen-specific IL-21 responses can be detected also by ELISA.

关键词： human IL21 human IL17 ELISpot ELISA

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