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人工调控受体聚集的化学合成生物学策略及应用

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合成生物学 2024年第1期5卷 53-76页

作者：袁燕燕陈慧芳杨思慧王洪辉聂舟湖南大学化学生物传感与化学计量学国家重点实验室化学化工学院生物学院生物大分子化学生物学湖南省重点实验室湖南长沙410082

细胞表面受体的聚集和激活在多种生物过程中发挥着重要作用,如细胞迁移、增殖、凋亡和分化。鉴于受体介导的细胞功能对健康和疾病的广泛相关性,研究人员一直致力于探究细胞受体信号传递和激活的生物化学和生物物理学机制,进而在其基础... 详细信息

细胞表面受体的聚集和激活在多种生物过程中发挥着重要作用,如细胞迁移、增殖、凋亡和分化。鉴于受体介导的细胞功能对健康和疾病的广泛相关性,研究人员一直致力于探究细胞受体信号传递和激活的生物化学和生物物理学机制,进而在其基础上开发多样的分子工程策略操纵受体信号和细胞功能。随着化学合成生物学的快速发展,一系列分子工程工具被开发出来用于理性控制受体,使得人工受体激活更加简单、精准和多样化。本综述首先总结了涉及调节受体聚集控制的关键功能元件,包括分子识别、空间组织、动态和细胞选择性元件。随后介绍了这些高度可控的功能模块在动态聚集、特定响应性、时空分辨和高细胞选择性的精准控制受体聚集分子工具的最新研究进展。此外强调了多种人工控制受体聚集的精准激活策略在细胞表型和命运操纵、免疫激活和活体组织修复方面的新兴应用。最后,本文从作用机理、元件工程、临床局限性、体内长效性等多个角度概述人工受体聚集策略当前面临的挑战和缺点。同时,本文也对其在疾病治疗领域的潜在应用进行了前瞻性的展望。

关键词：化学合成生物学受体聚集元件工程生物医学工程细胞调控

A photonanozyme with light-empowered specific peroxidasemimicking activity

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Nano Research 2022年第10期15卷 9073-9081页

作者： Sili Lin Wenlong Tan Pengfei Han Xu Li Jinzhao Li zhou nie Kun Li State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringHunan Provincial Key Laboratory of Biomacromolecular Chemical BiologyHunan UniversityChangsha 410082China

Although nanozymes have been widely developed,directly utilizing light to drive catalytic reactions like natural photoenzymes still remains ***,we propose that photonanozymes(PNZs),as a novel kind of nanozyme,exclusively possess enzyme-mimicking activity under *** in the presence of visible light,the as-synthesized TiO_(2) proposed in this contribution shows excellent specificity of peroxidase-like without any oxidase-or catalase-like *** driving force of the light-empowered peroxidase-like photonanozymatic activity is explicated in terms of the photogenerated hot charge carriers in TiO_(2) PNZs and the accompanied reactive oxygen *** co-substrates for photonanozymatic reaction over TiO_(2) PNZs facilitate the formation of the precarious and reactive peroxo-oxygen bridge between TiO_(2) and H_(2)O_(2),enabling the catalytic *** the TiO_(2) PNZ-based biosensing platform for visual glucose detection exemplifying the concept of the application of PNZs,this work may evoke more inspirations to explore strategies for enlarging the scope of photoenzyme mimics.

关键词： photonanozyme titanium dioxide peroxidase mimics specificity biosensors

Responsive MXene nanovehicles deliver CRISPR/Cas12a for boolean logic-controlled gene editing

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Science China Chemistry 2022年第11期65卷 2318-2326页

作者： Song Wang Zhenhua Zhang Rui Tang Fang He Yan Huang zhou nie Chunyang Lei Department State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringHunan Provincial Key Laboratory of Biomacromolecular Chemical BiologyHunan UniversityChangsha 410082China Hubei Yangtze Memory Laboratories Wuhan 430205China

Programmable and precise regulation of genetic information is crucial in bioengineering and biomedicine;however, it remains challenging to implement this objective. Here we deployed DNA-functionalized MXenes as a smart delivery system for spatiotemporally controllable genome editing. The MXene nanovehicles rationally integrated photothermal effect with nucleic acid strand displacement reaction, thereby allowing for the binary logic gate-controlled release of Cas ribonucleoprotein complexes in response to different input patterns of NIR light and nucleic acids. This system was highly programmable and could be harnessed to construct 2-input(AND, OR, and N-IMPLY) and 3-input(AND/OR and N-IMPLY/OR) logic gates for precise gene editing in mammalian cells. Moreover, an AND logic gate-controlled delivery system achieved selective induction of tumor cell death in a xenograft mice model using tissue-penetrating NIR light and cancer-relevant microRNA as the inputting ***, the MXene nanovehicles adopted both the external and endogenous signals as the stimuli to precisely control gene editing under logic computation, presenting a helpful strategy for therapeutic genome editing.

关键词： Mxenes boolean logic gates CRSIPR/Cas12a gene editing nanovehicles

Unraveling the Dynamics of Antibody-Antigen Interaction by DNA Origami

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Chemical Research in Chinese Universities 2020年第5期36卷 983-984页

作者： nie zhou State Key Laboratory of Chemo Bio-sensing and Chemometncs College of Chemistry and Chemical EngineeringHunan Provincial Key Laboratoty of Biomacromolecular Chemical BiologyHunan UniversityChangsha 410082P.R.China

To investigate the dynamic interaction between antibody and *** et *** designed a triangular DNA origami framework to spatially organize the antigenic epitopes at the naiioscale and thus to monitor the transient binding kinetics of thedynamic antigen-antibody complexes at room *** study provides a straightforward,desigiiable and programmable strategy to investigate the transition kinetics of antibody-antigen interaction at a single-molecule level and improve theunderstanding toward thedesign of the next-generation antibodies and vaccines lor various biomedical *** work has been published online in Nature Communications on June 19,2020.

关键词： temperature. kinetics interaction

Integrated interconnected porous and lamellar structures realized fast ion/electron conductivity in high-performance lithium-sulfur batteries

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Chinese Chemical Letters 2024年第6期35卷 161-167页

作者： Ya Song Mingxia zhou Zhu Chen Huali nie Jiao-Jing Shao Guangmin zhou School of Materials and Metallurgy Guizhou UniversityGuiyang 550025China Medical College of Guizhou University Guiyang 550025China College of Biological Science and Medical Engineering Donghua UniversityShanghai 201620China Shenzhen Geim Graphene Center Tsinghua Shenzhen International Graduate SchoolTsinghua UniversityShenzhen 518055China

The practical application of high-energy-density lithium-sulfur(Li-S)batteries have been highly praised for energy storage devices,while are largely hindered by the“shuttling effect”.Herein,core-shell carbon spheres composed of interlinked porous core and lamellar shell were designed to restrain the polysulfide *** microporous structure with pore size of around 1 nm effectively trap lithium ***,the interconnected porous core shortens the ion transfer distance and the lamellar carbon shell endows the carbon spheres with fast electron conduction,finally facilitating polysulfide conversion ***,the Li-S batteries with the carbon spheres as the interlayer show high discharge specific capacity of 1002 m Ah/g at 2 C with 574 m Ah/g remaining after 600 cycles,and high areal capacity of 5.48 m Ah/cm^(2) with sulfur loading of 4.67 mg/cm^(2) at 0.1 *** corresponding pouch cells also exhibit stable cycling stability with an initial discharge specific capacity of 1082 m Ah/g at 0.1 C.

关键词： Interlayer Shuttle effect Strong adsorption Porous carbon spheres Lithium-sulfur batteries

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基于大数据特征的形成性评价实施策略探索

计算机应用文摘 2024年第5期40卷 86-88页

作者：周聂陆军勤务学院教研保障中心重庆401331

随着大数据技术的飞速发展,教育逐渐成为大数据应用的重要领域。单一的传统教学模式逐渐与微课、慕课和翻转课堂等互联网教育模式相结合,目前已成为学术界和教学实践改革的热点话题。大数据为学生的学习评价提供了技术保障,形成性评价... 详细信息

随着大数据技术的飞速发展,教育逐渐成为大数据应用的重要领域。单一的传统教学模式逐渐与微课、慕课和翻转课堂等互联网教育模式相结合,目前已成为学术界和教学实践改革的热点话题。大数据为学生的学习评价提供了技术保障,形成性评价的优势得以彰显,使学习目标明晰化、技术指导个性化、教学反思深入化,为教学质量提升带来体系变革。文章对传统教学评价进行了特征分析,分析了大数据背景下形成性评价具备的典型特征,提出了应用大数据技术实施融入教学的形成性评价实施策略。

关键词：大数据教育形成性评价实践探索

Large-area ultrastrong and stiff aramid nanofiber based layered nanocomposite films

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Nano Research 2024年第2期17卷 829-835页

作者： Zhidong nie Yingzhi Sun Tianxu zhou Shengwen Kong Linxing Liu Chuangqi Zhao Mingjie Liu Key Laboratory of Bioinspired Smart Interfacial Science and Technology of Ministry of Education School of ChemistryBeihang UniversityBeijing 100191China University of Science and Technology of China Hefei 230026China Suzhou Institute for Advanced Research University of Science and Technology of ChinaSuzhou 215123China International Research Institute for Multidisciplinary Science Beihang UniversityBeijing 100191China

One-dimensional(1D)aramid nanofiber(ANF)based nanocomposite films have drawn increasing attentions in various applications due to their excellent mechanical properties and impressive chemical and thermal ***,the large-area fabrication of aramid nanocomposite films with ultrastrong mechanical properties under mild conditions remains a great *** we present a facile superspreading-assisted strategy to produce aramid nanofiber based oriented layered nanocomposites using phase inversion process that occurs at the fully swollen hydrogel *** nanocomposite films based on ANF,carboxylation carbon tube(CNT–COOH),poly(vinyl alcohol)(PVA),and MXene nanosheet exhibit a tensile strength of up to 870.8±85 MPa,a Young’s modulus of 21.8±2.2 GPa,and outstanding toughness(up to 43.2±4.6 MJ/m^(3)),which are much better than those conventional aramid nanofiber based *** conductivity of our nanocomposite films reaches the maximum of about 1100 S/*** fabulous mechanical properties combination and continuous production capability render our strategy representing a promising direction for the development of high-performance nanocomposites.

关键词： aramid nanofibers MXene nanosheets nanocomposites ultrastrong and stiff superspreading large-area production

Stability and transepithelial transport of oligopeptide(KRQKYD)with hepatocyte-protective activity from Jinhua ham in human intestinal Caco-2 monolayer cells

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Food Science and Human Wellness 2024年第3期13卷 1503-1512页

作者： Wen nie Feiran Xu Kai zhou Jieying Deng Ying Wang Baocai Xu Engineering Research Center of Bio-process Ministry of EducationSchool of Food and Biological EngineeringHefei University of TechnologyHefei 230601China School of Biological and Food Engineering Chuzhou UniversityChuzhou 239001China

The study evaluated the stability of an oligopeptide(Lys-Arg-Gln-Lys-Tyr-Asp,KRQKYD)and its transport mechanism by simulating gastrointestinal digestion and a model of human intestinal Caco-2 monolayer cells in *** this study,the effects of environmental factors(temperature,pH and NaCl concentration)and simulated gastrointestinal digestion on the stability of KRQKYD were evaluated by indicators of the levels of alanine transaminase(ALT),aspartate transaminase(AST)and malondialdehyde(MDA)in an alcoholinduced hepatocyte injury *** results showed that KRQKYD still maintained satisfactory hepatocyteprotective activity after treatment with different temperatures(20-80℃),pH(3.0-9.0),NaCl concentration(1%-7%)and simulated gastrointestinal digestion,which indicated that KRQKYD showed good stability to environmental factors and simulated gastrointestinal ***,the intact KRQKYD could be absorbed in a model of Caco-2 monolayer cells with a P_(app)value of(9.70±0.53)×10^(-7)cm/*** with an energy inhibitor(sodium azide),a competitive peptide transporter inhibitor(Gly-Pro)and a transcytosis inhibitor wortmannin did not decrease the level of transepithelial KRQKYD transport,indicating that the transport mechanism of KRQKYD was not associated with energy dependent,vector mediated and *** tight junction disruptor cytochalasin D significantly increased the level of transepithelial KRQKYD transport(P<0.05),suggesting that intact KRQKYD was absorbed by paracellular transport.

关键词： Jinhua ham KRQKYD(Lys-Arg-Gln-Lys-Tyr-Asp) Environmental stability Gastrointestinal digestive Transport mechanism

Reversed charge transfer induced by nickel in Fe-Ni/Mo_(2)C@nitrogen-doped carbon nanobox for promoted reversible oxygen electrocatalysis

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Journal of Energy Chemistry 2024年第1期88卷 202-212,I0005页

作者： Zhicheng nie Lei Zhang Qiliang Zhu Zhifan Ke Yingtang zhou Thomas Wågberg Guangzhi Hu School of Materials Science and Engineering Anhui University of Science and TechnologyHuainan 232001AnhuiChina Institute for Ecological Research and Pollution Control of Plateau Lakes School of Ecology and Environmental ScienceYunnan UniversityKunming 650504YunnanChina National Engineering Research Center for Marine Aquaculture Marine Science and Technology CollegeZhejiang Ocean UniversityZhoushan 316004ZhejiangChina Department of Physics UmeåUniversityUmeåS-90187Sweden

The interaction between metal and support is critical in oxygen catalysis as it governs the charge transfer between these two entities,influences the electronic structures of the supported metal,affects the adsorption energies of reaction intermediates,and ultimately impacts the catalytic *** this study,we discovered a unique charge transfer reversal phenomenon in a metal/carbon nanohybrid ***,electrons were transferred from the metal-based species to N-doped carbon,while the carbon support reciprocally donated electrons to the metal domain upon the introduction of *** led to the exceptional electrocatalytic performances of the resulting Ni-Fe/Mo_(2)C@nitrogen-doped carbon catalyst,with a half-wave potential of 0.91 V towards oxygen reduction reaction(ORR)and a low overpotential of 290 m V at 10 mA cm^(-2)towards oxygen evolution reaction(OER)under alkaline ***,the Fe-Ni/Mo_(2)C@carbon heterojunction catalyst demonstrated high specific capacity(794 mA h g_(Zn)~(-1))and excellent cycling stability(200 h)in a Zn-air *** calculations revealed that Mo_(2)C effectively inhibited charge transfer from Fe to the support,while secondary doping of Ni induced a charge transfer reversal,resulting in electron accumulation in the Fe-Ni alloy *** local electronic structure modulation significantly reduced energy barriers in the oxygen catalysis process,enhancing the catalytic efficiency of both ORR and ***,our findings underscore the potential of manipulating charge transfer reversal between the metal and support as a promising strategy for developing highly-active and durable bi-functional oxygen electrodes.

关键词： Metal-support interaction Charge transfer reversal Oxygen reduction reaction Oxygen evolution reaction Zinc-air battery