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Transcriptomic responses to drought stress in the Patagonian southern beech Nothofagus alpina

Ecological Processes 2024年第2期13卷 192-203页

作者： Rita Maria Lopez Laphitz María Verónica Arana santiago Agustín Varela Leandro Aníbal Becker Carolina soliani María Marta Azpilicueta Paula Marchelli nicolás.bellora Instituto de Diversidad y Evolución Austral (CENPAT CONICET ) Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB INTA CONICET ) Instituto de Tecnologías Nucleares para la salud (CONICET )

Background Deciphering the genetic architecture of drought tolerance could allow the candidate genes identification responding to water stress. In the Andean Patagonian forest, the genus Nothofagus represents an ecologically relevant species to be included in different genomic studies. These studies are scarce in south American ecosystems however represent an important source of genomic data in order to interpret future climate-change environment scenarios of these emblematic forests. Here, we achieved the assemblage of the transcriptome of N. alpina while searching for key genes of activated or suppressed metabolic pathways in response to drought *** De novo transcriptome assembly resulted in 104,030 transcripts. Following confirmation of drought conditions, based on reduction of leaf water potential and stomatal conductance, a differential gene expression analysis resulted in 2720 significantly expressed genes(1601 up-regulated and 1119 down-regulated). Enrichment analysis(over-representation analysis and gene set enrichment analysis) resulted in more than one hundred stress-responsive term ontologies(i.e. biological processes) and pathways. Terms such as response to abscisic acid and pathways such as plant hormone signal transduction or starch and sucrose metabolism were over-represented. Protein–protein interaction assessment resulted in networks with significantly expressed top common hub gene clusters(e.g. planttype cell wall biogenesis among down-regulated or ABA-signalling among up-regulated). These networks evidenced important regulators at gene expression such as transcriptional *** Responses of N. alpina seedlings to drought stress were evidenced by the activation of several genes linked to GO biological processes and KEGG pathways, which were mainly based on over-expression of specific protein kinases, phosphatases, synthases and transcription factors. This suggests an up-regulation of signalling pathways, triggered through pla

关键词： Climate change Differential gene expression Hub genes Patagonia Temperate forest

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Temperature-Programmed Reduction and Dispersive X-Ray Absorption spectroscopy studies of CeO<sub>2</sub>-Based Nanopowders for Intermediate-Temperature solid-Oxide Fuel Cell Anodes

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Materials sciences and Applications 2019年第10期10卷 631-642页

作者： Marina s. bellora Joaquí n sacanell Cristiá n Huck-Iriart Analí a L. soldati susana A. Larrondo Diego G. Lamas CONICET/Laboratorio de Cristalografía Aplicada Escuela de Ciencia y Tecnología Universidad Nacional de General San Martín San Martín Argentina Departamento de Física de la Materia Condensada Gerencia de Investigación y Aplicaciones Centro Atómico Constituyentes Comisión Nacional de Energía Atómica San Martín Argentina Instituto de Nanociencia y Nanotecnología (INN) CNEA-CONICET San Martín and San Carlos de Bariloche Argentina UNIDEF-CONICET-MINDEF Departamento de Investigaciones en Sólidos CITEDEF Villa Martelli Argentina Instituto de Investigación e Ingeniería Ambiental Universidad Nacional de General San Martín San Martín Argentina

In this work, we study the influence of the average crystallite size and dopant oxide on the reducibility of CeO2-based nanomaterials. samples were prepared from commercial Gd2O3-, sm2O3- and Y2O3-doped CeO2 powders by calcination at different temperatures ranging between 400°C and 900°C and characterized by X-ray powder diffraction, transmission electron microscopy and BET specific surface area. The reducibility of the samples was analyzed by temperature-programmed reduction and in situ dispersive X-ray absorption spectroscopy techniques. Our results clearly demonstrate that samples treated at lower temperatures, of smallest average crystallite size and highest specific surface areas, exhibit the best performance, while Gd2O3-doped ceria materials display higher reducibility than sm2O3- and Y2O3-doped CeO2.

关键词： Crystallite size CeO2-Based Nanomaterials sm2O3

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Evaluation of cats(Felis catus)as possible asymptomatic carriers of dermatophytes in extreme south of Brazil

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Journal of Coastal Life Medicine 2015年第5期 370-371页

作者： Tony silveira Mariana Remiao Camila Graeff Fabiana Poetsch Guilherme Azevedo Juliane Guimaraes Rafaela bellora Tassiane Moraes Pedro Quevedo Renata de Faria Postgraduate Program in Biotechnology Federal University of PelotasPelotasRSBrasil Faculty of Veterinary Medicine Federal University of PelotasPelotasRSBrasil Postgraduate Program in Parasitology Federal University of PelotasPelotasRSBrasil

Objective:To investigate the presence of Microsporum canis in pelage of asymptomatic cats for dermatophytosis,in south region of Rio Grande do sul,Brazil,and evaluate its importance in epidemiology of dermatophytosis in the study ***:A total of 60 domestic cats were evaluated for the presence of Microsporum *** animals were divided into three groups of 20 *** group consisted of exclusively domiciled,semi-domiciled and rural *** were collected following the carpet-square *** microorganisms were cultivated under laminar flow in mycosel agar and grown in a ***:All the cats of the three groups analysed had negative cultures for *** 85%of the dishes,there was a growth of environmental saprophytic fungi such as Aspergillus sp.,Fusarium *** Penicillium ***:Thus,asymptomatic cats for dermatophytes did not show importance in the transmission and maintenance of the disease in southern of Rio Grande do sul,Brazil.

关键词： Dermatophytosis Epidemiology Fur

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