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Agrobacterium-Mediated transformation of Cry8db Gene in Vietnam Sweet Potato Cultivar

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Journal of Life Sciences 2015年第6期9卷 262-271页

作者： Pham Bich ngoc vu Thi Lan tran Thu trang Nguyen Hoai Thuong Le Thu ngoc Chu Hoang Ha Le tran Binh Institute of Biotechnology Vietnam Academy of Science and Technology 18-Hoang Quoc Viet Cau Giay Ha Noi 10000 Vietnam College of Science Thai Nguyen University Tan Thinh Thai Nguyen 23000 Vietnam University of Science and Technology of Ha Noi 18-Hoang Quoc Viet Cau Giay Ha Noi 10000 Vietnam

Sweet potato [Ipomoea batatas （L.） Lam.] is an important food crop in the world as well as in Vietnam. It is well known as a recalcitrant crop for gene transformation and tissue culture because of its genotype dependent in vitro responses. In present study, Agrobacterium-mediated transformation of cry8Db from Bacillus thuringiensis into KB 1 sweet potato variety has been studied. The C58cv strain carrying a pBl 121 backbone which contained cry8Db delta-endotoxin gene regulated under 35 S CaMV prom oter, and the selection marker gene, neomycin phosphotransferase （npt11） gene, was subjected for plant transformation. Callus induced from shoot tips and leaf explants were inoculated and cocultured with A. tumefaciens. The selection occurred during callus producing and plant regenerating steps. A total of 201 transgenic putative plant lines were produced, and 21 transgenic lines were positively confirmed by PCR and finalized by Southern blot. Four putative transgenic lines confirming a single copy of the crySEIb gene were transferred into soil pots in greenhouse. Biological activity evaluation for the insecticidal capacity of these transgenic lines under controlled conditions showed that the level of infestation by sweet potato weevils （Cylasformicarius） in untransformed plants was higher than that of transgenic lines.

关键词： Sweet potato Agrobacterium tumefaciens plant transformation cry8Db sweet potato weevil resistance cylas formicarius.

Knowledge and attitude toward COVID-19 among healthcare workers at District 2 Hospital, Ho Chi Minh City

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Asian Pacific Journal of Tropical Medicine 2020年第6期13卷 260-265页

作者： Huynh Giao Nguyen Thi ngoc Han tran Van Khanh Vo Kim Ngan Vo Van Tam Pham Le An Faculty of Public Health University of Medicine and Pharmacy at Ho Chi Minh CityVietnam Department of Infectious Diseases University Medical Center Ho Chi Minh CityVietnam Department of Scientific Research District 2 HospitalHo Chi Minh CityVietnam Department of Nursing District 2 HospitalHo Chi Minh CityVietnam Faculty of Nursing and Medical Technology University of Medicine Pham Ngoc Thach at Ho Chi Minh CityVietnam Center for the Training of Family Medicine Faculty of MedicineUniversity of Medicine and Pharmacy at Ho Chi Minh CityVietnam

Objective:To assess the knowledge and attitude toward coronavirus disease-2019(COVID-19)among healthcare workers at District 2 Hospital in Ho Chi Minh City(HCMC).Methods:A cross-sectional study was performed between January 2020 and February 2020 at District 2 Hospital.A systematic random sampling strategy was carried out and the data was collected through a self-administered questionnaire of the knowledge and attitude of healthcare workers regarding *** analysis was reported to describe the demographic,mean knowledge and attitude score of healthcare *** statistics including t-test,ANOVA and Spearman’s correlation were used to evaluate the relationship between study ***:A total of 327 eligible healthcare workers had a mean score of knowledge and attitude of 8.17±1.3(range 4-10)and 1.86±0.43(range 1-5),*** showed good knowledge and a positive ***,approximately two thirds of the participants knew the mode of transmission,the isolation period and treatment(67.0%,65.8%,and 58.4%,respectively),and 82.3%and 79.8%,respectively,held positive attitude regarding the risk of personal and family members getting *** was a negative correlation between knowledge scores and attitude scores(r=-0.21,P<0.001).Additionally,healthcare workerspredominately used social media to inform themselves about COVID-19(91.1%).Conclusions:The majority of healthcare workers had good knowledge and positive attitude toward ***,the level of some knowledge and attitude lower than that expected for their position level towards the *** education interventions and campaigns are required for healthcare workers.

关键词： Knowledge Attitude COVID-19 Healthcare worker District 2 Hospital

Association between the Phenotypes and Genotypes of Antimicrobial Resistance in Haemophilus parasuis Isolates from Swine in Quang Binh and Thua Thien Hue Provinces, Vietnam

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Engineering 2020年第1期6卷 40-48页

作者： Chao Nguyen Van Lijun Zhang Tam vu Thi Thanh Hung Pham Hoang Son Tuan tran ngoc Qi Huang Rui Zhou State Key Laboratory of Agricultural Microbiology College of Veterinary MedicineHuazhong Agricultural UniversityWuhan 430070China Faculty of Animal Science and Veterinary Medicine University of Agricultural and ForestryHue UniversityHue 53000Vietnam Mientrung Institute for Scientific Research Vietnam Academy of Science and TechnologyHue 53000Vietnam Guangdong Provincial Key Laboratory of Marine Biology Shantou UniversityShantou 515063China Cooperative Innovation Center of Sustainable Pig Production Wuhan 430070China International Research Center for Animal Diseases The Ministry of Science and TechnologyWuhan 430070China

Haemophilus parasuis (H. parasuis) is one of the bacterial pathogens of great concern as it causes huge economic losses to the swine industry worldwide. One of the reasons why the control of H. parasuis has failed is the increase in antimicrobial resistance (AMR). The country of Vietnam has the second-largest pig production in Asia. However, there is still a lack of data about the AMR prevalence of H. parasuis in *** purpose of this study is to investigate the prevalence of AMR and analyze the association between AMR and AMR genes (ARGs). The H. parasuis strains used in this research were isolated from swine in the Quang Binh and Thua Thien Hue Provinces, Central Vietnam, as reported in our previous study. All of the strains were tested for AMR against 25 antibacterial agents using the broth microdilution method and for the presence of ARGs using the polymerase chain reaction (PCR) method. The tested strains were shown to have a high frequency of resistance to trimethoprim/sulfamethoxazole (94.6%), followed by resistance to colistin, chloramphenicol, gentamicin, penicillin, lincomycin, and amoxicillin. The most prevalent ARGs in these strains were blaTEM-1 (94.6%), int (76.8%), gyrA (58.9%), and rmtD (50.0%). Cefuroxime, chloramphenicol, and tobramycin resistances were strongly correlated with the presence of the ARGs blaROB-1 (odds ratio (OR) = 26.3, 95% confidence interval (CI) 2.7–255.7, p = 0.002), catl (OR = 25.1, 95% CI 2.4–258.9, p = 0.004), and strB (OR = 23.5, 95% CI 2.6–212.6, p = 0.001), *** study reveals for the first time the current situation of H. parasuis AMR in Central Vietnam, which is helpful for the clinical control of this disease, as well as for the development of policies and clinical practice guidelines to reduce AMR in swine production in Central Vietnam.

关键词： Haemophilus parasuis Antimicrobial resistance Antimicrobial resistance gene Vietnam

Treatment for COVID-19 patients in Vietnam: Analysis of time-to-recovery

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Asian Pacific Journal of Tropical Medicine 2020年第9期13卷 397-401页

作者： Khuong Quynh Long Hoang Hong Hanh tran Thi Tuyet Hanh La ngoc Quang Hoang Van Minh Hanoi University of Public Health 1A Duc Thang RoadDuc Thang WardNorth Tu Liem DistrictHanoiVietnam

Objective:To describe the recovery time and related factors among COVID-19 patients in ***:We used the secondary data obtained from the official database of the Ministry of Health of Vietnam and other public data sources that were available by April 9 th,*** proportional hazards model was carried out to identify factors related to recovery time among COVID-19 ***:By April 9 th,2020,the cumulative number of COVID-19 cases detected in Vietnam was 255,of which 129(50.6%)patients had fully *** median recovery time of patients was 17(95%CI=16-19)*** patients had a lower likelihood of recovery(HR=0.98,95%CI=0.97-0.99,P<0.001),whereas patients with a history of international incoming travel had a higher likelihood of recovery(HR=1.57,95%CI=1.03-2.40,P=0.036).There was no statistically significant difference in the recovery time of patients treated in different hospital ***:More attention is needed for older patients and who did not have international travel *** confirmed with COVID-19 could be treated at local health facilities to avoid unnecessary referrals and burdens to specialized hospitals at the central level.

关键词： COVID-19 Coronavirus Recovery Vietnam

Behavior of noncircular tunnels excavated in stratified rock masses-Case of underground coal mines

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Journal of Rock Mechanics and Geotechnical Engineering 2019年第1期11卷 99-110页

作者： ngoc Anh Do Daniel Dias Van Diep Dinh Tien Tung tran Van Canh Dao Viet Doan Dao Phuc Nhan Nguyen Department of Underground and Mining Construction.Faculty of Civil Engineering Hanoi University of Mining and Ceology School of Automotive and transportation Engineering Hefei University of Technology Laboratory 35R Crenoble Alpes University

The amount of tunnels excavated along stratified/sedimentary rock masses in Quangninh coal mine area,Vietnam, is gradually increasing. Rock mass in Quangninh is characterized by beddings between rock layers. The behavior of stratified rock masses surrounding the tunnels depends on both the intact rock and the beddings between rock layers. The main characteristics of stratified rock masses that need to be considered are their heterogeneity and anisotropy. Depending on the dip angle of rock layers, movements and failure zones developed surrounding the tunnels can be asymmetrical over the vertical axis of tunnel. This asymmetry causes adverse behaviors of the tunnel structures. The objective of this study is to highlight convergences and yielded zones developed in rock masses surrounding noncircular tunnels in Quangninh coal mine area using a finite element method. The presence of bedding joints is explicitly simulated. The numerical results indicated that with the increase in dip angle of bedding joints, the stress asymmetry over the tunnel vertical axis increases. It gradually leads to an asymmetry of the failure zone surrounding the tunnel. An increase of rock mass quality means a decrease of rock mass sensitivity to the discontinuities. In addition,a dip angle of the bedding joints of approximately 45° could be considered as the critical angle at which the rock mass mechanism changes between sliding and bending.

关键词： Tunnel Bedding Stratification Anisotropic Heterogeneity

Favipiravir and its potentials in COVID-19 pandemic:An update

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Asian Pacific Journal of Tropical Medicine 2021年第10期14卷 433-455页

作者： Dao ngoc Hien Tam Ahmad T Qarawi Mai ngoc Luu Morgan Turnage Linh tran Gehad Mohamed Tawfik Le Huu Nhat Minh Nguyen Tien Huy Tatsuo Iiyama Kyoshi Kita Kenji Hirayama Online Research Club School of Tropical Medicine and Global HealthNagasaki UniversityNagasaki 852-8523Japan Asia Shine Trading&Service CO.LTD. Ho Chi Minh City 700000Vietnam Essen Healthcare BronxNew York 10461 USA Department of Internal Medicine University of Medicine and Pharmacy at Ho Chi Minh CityHo Chi Minh City 700000Vietnam American University of the Caribbean CupecoySint Maarten Institute of Fundamental and Applied Sciences Duy Tan UniversityHo Chi Minh City 700000Vietnam Faculty of Natural Sciences Duy Tan UniversityDa Nang City 550000Vietnam Department of Otorhinolaryngology Faculty of MedicineAin Shams UniversityCairoEgypt Methodist Hospital MerrillvilleIndianaUnited States School of Tropical Medicine and Global Health Nagasaki University1-12-4 SakamotoNagasaki 852-8523Japan Department of International Trials Center of Clinical SciencesNational Center for Global Health and Medicine(NCGM)TokyoJapan

The COVID-19 pandemic has caused millions of deaths and hundreds of millions of confirmed infections *** pandemic has prompted researchers to produce medications or vaccines to reduce or stop the progression and spread of this disease.A variety of previously licensed and marketed medications are being tested for the treatment and recurrence of SARS-CoV2,including favipiravir(Avigan).Favipiravir was recognized as an influenza antiviral drug in Japan in 2014,and has been known to have a potential in vitro activity against SARS-CoV-2,in addition to its broad therapeutic safety *** was recently approved and officially used in many countries *** review provides insights and up-to-date knowledge of the current role of favipiravir in the treatment of COVID-19 infection,focusing on preclinical and ongoing clinical trials,evidence of its efficacy against SARS-CoV-2 in COVID-19,side effects,anti-viral mechanism,and the pharmacokinetic properties of the drug in the treatment of *** to its teratogenic effects,favipiravir cannot be offered to expectant or pregnant *** practical efficacy of such an intervention regimen will depend on its dose,treatment duration,and cost as well as difficulties in application.

关键词： Favipiravir Avigan COVID-19 SARS-CoV-2 Antiviral Review Efficacy

The COVID-19 pandemic in the ASEAN: A preliminary report on the spread, burden and medical capacities

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Asian Pacific Journal of Tropical Medicine 2020年第6期13卷 247-251页

作者： Minh Van Hoang Phuong Thi ngoc Nguyen Thao Thi Phuong tran Long Quynh Khuong An Thi Minh Dao Huy Van Nguyen Riyanti Djalante Hanh Thi Tuyet tran Hanoi University of Public Health HanoiVietnam Hanoi Medical University HanoiVietnam Graduate School of Public Health St.Luke's International UniversityTokyoJapan United Nations University-Institute for the Advanced Study of Sustainability(UNU-IAS) TokyoJapan

Objective:To provide preliminary descriptions of the spread,burden and related medical capacity characteristics of the ASEAN ***:We utilized the data from four main official databases,including WHO reports,the Statistics and Research of the Coronavirus Disease,and the Southeast Asia Program of the Center for Strategic and International *** spread of the COVID-19 pandemic,current burden and the COVID-19 medical response capacities were extracted before April 11,***:As of April 13,2020,the ASEAN countries reported19547 COVID-19 positive cases with 817 deaths(case-facility rate of 4.2%).Thailand was the first country in the region that reported having the COVID-19 cases,while Laos was the last to report confirmed COVID-19 *** for the number of deaths to double were between 8-12 *** the testing and treatment capacities,the number of PCR tests provided to the populations was the highest in Vietnam,followed by Singapore,Malaysia,and ***,the percentage of the population being tested was the highest in Brunei(2.31%),followed by Singapore(1.30%).Conclusions:A wide range of interventions were taken into practice in response to the outbreak with an effort of curbing the rise of this ***,special care should not be overlooked as the pandemic is placing a huge impact on the population and becomes increasingly *** tackle the spread of the pandemic in the region,the ASEAN countries should work together as one community to provide better responses to future pandemics and other transboundary public health challenges.

关键词： COVID-19 SARS-CoV-2 ASEAN South-East Asia Epidemiology

Shape dependent protein-induced stabilization of gold nanoparticles:From a protein corona perspective Special Collection:Distinguished Australian Researchers

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聚集体(英文)

聚集体(英文) 2023年第4期4卷 177-184页

作者： Anastasiia Tukova Yihan Nie Mohammad Tavakkoli Yaraki ngoc Thanh tran Jiaqiu Wang Alison Rodger Yuantong Gu Yuling Wang School of Natural Sciences Faculty of Science and EngineeringMacquarie UniversitySydneyNew South WalesAustralia School of Mechanical Medical and Process EngineeringQueensland University of Technology(QUT)BrisbaneQueenslandAustralia Centre for Biomedical Technologies Queensland University of Technology(QUT)BrisbaneQueenslandAustralia Centre for Materials Science Queensland University of Technology(QUT)BrisbaneQueenslandAustralia

Gold nanoparticles(AuNPs)are promising materials for many ***,upon contacting with biological media,AuNPs undergo *** interaction with proteins results in the so-called protein corona(PC)around AuNPs,leading to the new bioidentity and optical *** the mechanisms of PC formation and its functions can help us to utilise its benefits and avoid its *** date,most of the previous works aimed to understand the mechanisms governing PC formation and focused on the spherical nanoparticles,although non-spherical nanoparticles are designed for a wide range of applications in *** this work,we investigated the differences in PC formation on spherical and anisotropic AuNPs(nanostars in particular)from the joint experimental(extinction spectroscopy,zeta potential and surface-enhanced Raman scattering[SERS])and computational methods(the finite element method and molecular dynamics[MD]simulations).We discovered that protein does not fully cover the surface of anisotropic nanoparticles,leaving SERS hot-spots at the tips and high curvature edges‘available’for analyte binding(no SERS signal after pre-incubation with protein)while providing protein-induced stabilization(indicated by extinction spectroscopy)of the AuNPs by providing a protein layer around the particle’s *** findings are confirmed from our MD simulations,the adsorption energy significantly decreases with the increased radius of curvature,so that tips(adsorption energy:2762.334 kJ/mol)would be the least preferential binding site compared to core(adsorption energy:11819.263 kJ/mol).These observations will help the development of new nanostructures with improved sensing and targeting ability.

关键词： anisotropic gold nanoparticles molecular dynamics simulation protein corona SERS stability

Spectroscospy method for determination of Thorium with Azocalix[4]arene

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Spectroscospy method for determination of Thorium with Azoca...