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用LC-MS/MS法同时测定大鼠血浆中多黏菌素B和替加环素的浓度

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中国临床药理学杂志 2024年第7期40卷 1049-1053页

作者：韩竺杭刘玥李文利郑天伦杨秀岭河北医科大学第二医院药学部河北石家庄050000

目的建立大鼠血浆中多黏菌素B和替加环素测定的液相色谱串联质谱(LC-MS/MS)方法,并研究其在大鼠体内的药代动力学特征。方法用3%三氯乙酸-甲醇溶液(50:50)对大鼠血浆进行沉淀蛋白处理。色谱柱:Symmetry C_(18)(150.0mm×4.6mm,3.5μm),... 详细信息

目的建立大鼠血浆中多黏菌素B和替加环素测定的液相色谱串联质谱(LC-MS/MS)方法,并研究其在大鼠体内的药代动力学特征。方法用3%三氯乙酸-甲醇溶液(50:50)对大鼠血浆进行沉淀蛋白处理。色谱柱:Symmetry C_(18)(150.0mm×4.6mm,3.5μm),流动相:0.1%甲酸水-0.1%甲酸乙腈,流速:0.6mL·min^(-1),柱温:40℃;离子源:电喷雾离子源,正离子检测模式,多反应检测。考察该方法的专属性、标准曲线和定量下限、精密度与回收率、稳定性以及重现性。结果替加环素的线性范围为25~2500ng·mL^(-1),定量下限为25ng·mL^(-1),提取回收率为95.89%~107.90%;多黏菌素B_(1+1-I)的线性范围为82~8200ng·mL^(-1),定量下限为82ng·mL^(-1),提取回收率为93.84%~97.70%;多黏菌素B_(2)的线性范围为9~900ng·mL^(-1),定量下限为9ng·mL^(-1),提取回收率为96.41%~104.80%;各个物质的日内、日间相对标准偏差均小于10%,稳定性和重现性良好。结论本方法操作简单、灵敏度高、分析时间短,适用于大鼠血浆中多黏菌素B和替加环素的血药浓度测定以及药代动力学的研究。

关键词：多黏菌素B 替加环素液相色谱串联质谱药代动力学生物样本测定

Effect of Optimized Concentrations of Basic Fibroblast Growth Factor and Epidermal Growth Factor on Proliferation of Fibroblasts and Expression of Collagen： Related to Pelvic Floor Tissue Regeneration

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Chinese Medical Journal 2018年第17期131卷 2089-2096页

作者： Yuan-Yuan Jia Jing-Yi Zhou yue Chang Fang An Xiao-Wei Li Xiao-yue Xu Xiu-Li Sun Chun-Yang Xiong Jian-Liu Wang Department of Obstetrics and Gynecology Peking University People's Hospital Beijing 100044 China Beijing Key Laboratory of Female Pelvic Floor Disorders Beijing 100044 China Department of Mechanics and Bioengineering College of Engineering Peking University Beijing 100871 China

Background： Fibroblasts were the main seed cells in the studies of tissue engineering of the pelvic floor ligament. Basic fibroblast growth factor （bFGF） and epidermal growth factor （EGF） were widely studied but at various concentrations. This study aimed to optimize the concentrations of combined bFGF and EGF by evaluating their effects on proliferation and collagen secretion of fibroblasts. Methods： Fibroblasts were differentiated from rat adipose mesenchymal stem cells （ADSCs）. Flow cytometry and immunohistochemistry were used for cell identification. The growth factors were applied at concentrations of 0, 1, 10, and 100 ng/ml as three groups：（1） bFGF alone, （2） EGF alone, and （3） bFGF mixed with EGF. Cell proliferation was evaluated by Cell Counting kit-8 assays. Expression of Type I and III collagen （Col-I and Col-1II） mRNAs was evaluated by real-time quantitative reverse transcription-polymerase chain reaction. Statistical analysis was performed with SPSS software and GraphPad Prism using one-way analysis of variance and multiple t-test. Results： ADSCs were successfully isolated from rat adipose tissue as identified by expression of typical surface markers CD29, CD44, CD90, and CD45 in flow cytometry. Fibroblasts induced from ADSC, compared with ADSCs, were with higher mRNA expression levels of Col I and Col III （F = 1.29, P = 0.0390）. bFGF, EGF, and the mixture ofbFGF with EGF can enhanced fibroblasts proliferation, and the concentration of 10 ng/ml of the mixture ofbFGF with EGF displayed most effectively （all P 〈 0.05）. The expression levels of Col-I and Col-Ill mRNAs in fibroblasts displayed significant increases in the 10 ng/ml bFGF combined with EGF group （all P 〈 0.05）. Conclusions： The optimal concentration of both bFGF and EGF to promote cell proliferation and collagen expression in fibroblasts was 10 ng/ml at which fibroblasts grew faster and secreted more Type I and III collagens into the extracellular matrix, which might contribute to the stabilit

关键词： Adipose Mesenchymal Stem Cells Basic Fibroblast Growth Factor Epidermal Growth Factor Fibroblasts Tissue Engineering

HPLC-MS/MS法同时测定大鼠回肠中5-羟色胺和5-羟吲哚乙酸含量

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中国医院药学杂志 2024年第14期44卷 1651-1656页

作者：刘玥韩竺杭袁叶王丽楠张钰恬杨秀岭河北医科大学第二医院药学部河北石家庄050000

目的:建立同时测定大鼠回肠中5-羟色胺和5-羟吲哚乙酸含量的方法。方法:采用高效液相色谱-串联质谱(HPLC-MS/MS)法进行检测。色谱柱为X Bridge BEH C_(18)柱(100 mm×2.1 mm,2.5μm);流动相为0.1%甲酸水(A)-0.1%甲酸乙腈(B),梯度洗脱;... 详细信息

目的:建立同时测定大鼠回肠中5-羟色胺和5-羟吲哚乙酸含量的方法。方法:采用高效液相色谱-串联质谱(HPLC-MS/MS)法进行检测。色谱柱为X Bridge BEH C_(18)柱(100 mm×2.1 mm,2.5μm);流动相为0.1%甲酸水(A)-0.1%甲酸乙腈(B),梯度洗脱;流速为0.3 m L·min^(-1);柱温为40℃;进样量为5μL;电喷雾电离源(ESI),正离子模式下多反应监测。结果:5-羟色胺在25~1 000 ng·m L^(-1)范围内线性关系良好(r^(2)=0.999 1),定量下限为25 ng·m L^(-1);5-羟吲哚乙酸在50~2 000 ng·m L^(-1)范围内线性关系良好(r^(2)=0.999 5),定量下限为50 ng·m L^(-1)。5-羟色胺和5-羟吲哚乙酸的日内、日间精密度均小于10%,准确度在96.90%~105.22%范围内,提取回收率均大于90%,稳定性良好,基质影响较小。测得大鼠回肠中5-羟色胺的含量为(2 533.62±225.08) ng·g^(-1),5-羟吲哚乙酸的含量为(1 365.19±69.18) ng·g^(-1),5-羟色胺的转化率为0.54±0.03。结论:该实验建立的方法准确灵敏,快捷高效,能够准确测定大鼠回肠中5-羟色胺和5-羟吲哚乙酸的含量,为探究大鼠回肠中5-羟色胺的代谢情况提供可靠的方法支持。

关键词： HPLC-MS/MS 5-羟色胺 5-羟吲哚乙酸回肠神经递质

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蒙药材蜀葵花质量标准研究

北方药学 2024年第2期21卷 1-3,11页

作者：岳海塔娜高寒郭宝凤内蒙古自治区药品检验研究院内蒙古呼和浩特010020 内蒙古自治区中蒙药标准研究重点实验室内蒙古呼和浩特010020

目的:建立蒙药材蜀葵花的定性定量评价方法。方法:采用显微法、薄层色谱法建立蜀葵花的定性鉴别方法,2020年版《中国药典》方法测定水分、总灰分、酸不溶性灰分、浸出物、含量测定,采用高效液相色谱法建立蜀葵花中山柰素的定量分析方法... 详细信息

目的:建立蒙药材蜀葵花的定性定量评价方法。方法:采用显微法、薄层色谱法建立蜀葵花的定性鉴别方法,2020年版《中国药典》方法测定水分、总灰分、酸不溶性灰分、浸出物、含量测定,采用高效液相色谱法建立蜀葵花中山柰素的定量分析方法。结果:建立的薄层色谱法斑点清晰,重复性好,可用于对7批蜀葵花药材的定性鉴别。测得7批样品水分、总灰分、酸不溶性灰分、浸出物含量。蜀葵花中山柰素含量测定的标准曲线为Y=0.0471X-0.0288,线性范围为10.81ng~135.13 ng,相关系数为0.9999,精密度RSD=0.87%,加样回收率为102.03%,RSD=1.82%。7批蜀葵花中山柰素含量为0.12%~0.49%。结论:建立的显微法、薄层色谱法可用于对蜀葵花药材的定性鉴别,高效液相色谱法简便、可行,可用于蜀葵花中山柰素的含量测定。

关键词：蜀葵花山柰素显微鉴别法薄层色谱鉴别法高效液相色谱法质量标准

Structure modification,antialgal,antiplasmodial,and toxic evaluations of a series of new marine-derived 14-membered resorcylic acid lactone derivatives

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Marine Life Science & Technology 2022年第1期4卷 88-97页

作者： Wei-Feng Xu Na-Na Wu Yan-Wei Wu yue-Xuan Qi Mei-Yan Wei Laura M.Pineda Michelle G.ng Carmenza Spadafora Ji-Yong Zheng Ling Lu Chang-Yun Wang Yu-Cheng Gu Chang-Lun Shao Key Laboratory of Marine Drugs the Ministry of Education of ChinaSchool of Medicine and PharmacyOcean University of ChinaQingdao266003China Laboratory for Marine Drugs and Bioproducts Qingdao National Laboratory for Marine Science and TechnologyQingdao266237China State Key Laboratory for Marine Corrosion and Protection Luoyang Ship Material Research Institute(LSMRI)Qingdao266237China Center of Cellular and Molecular Biology of Diseases Instituto de Investigaciones Científcas y Servicios de Alta TecnologíaCity of KnowledgeClaytonApartado0816-02852Panama Syngenta Jealott’s Hill International Research Centre BracknellBerkshireRG426EYUK

Marine natural products play critical roles in the chemical defense of many marine organisms and are essential,reputable sources of successful drug ***-seven 14-membered resorcylic acid lactone derivatives 3–27 and 30–71 of the natural product zeaenol(1)isolated from the marine-derived fungus Cochliobolus lunatus were semisynthesized by chlorination,acylation,esterification,and acetalization in one to three *** structures of these new derivatives were established by HRESIMS and NMR *** the compounds(1–71)were evaluated for their antialgal and antiplasmodial *** them,14 compounds displayed antifouling activities against adhesion of the fouling *** particular,9 and 34 exhibited strong and selective inhibitory effects against the diatoms Navicula laevissima and Navicula exigua(EC50=6.67 and 8.55μmol/L),respectively,which were similar in efficacy to those of the positive control SeaNine 211(EC50=2.90 and 9.74μmol/L).More importantly,38,39,and 69–71 showed potent antiplasmodial activities against Plasmodium falciparum with IC50 values ranging from 3.54 to 9.72μmol/*** interestingly,the five antiplasmodial derivatives displayed non-toxicity in the cytotoxicity assays and the zebrafish embryos model,thus,representing potential promising antiplasmodial drug *** preliminary structure–activity relationships indicated that biphenyl substituent at C-2,acetonide at positions C-5′and C-6′,and tri-or tetra-substituted of acyl groups increased the antiplasmodial ***,combining evaluation of chemical ecology with pharmacological models will be implemented as a systematic strategy,not only for environmentally friendly antifoulants but also for structurally novel drugs.

关键词： Marine natural product 14-Membered resorcylic acid lactone Zeaenol Semisynthesis Antialgal activity Antiplasmodial activity

快速免疫组化在前列腺穿刺活检组织冰冻病理诊断中应用的初步经验

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中华泌尿外科杂志 2024年第5期45卷 379-385页

作者：熊帅涛王淼刘龙腾吕政通侯惠民陈佳丁鑫王悦张伟刘明北京医院泌尿外科、国家老年医学中心、中国医学科学院老年医学研究院北京100730 北京医院病理科、国家老年医学中心、中国医学科学院老年医学研究院北京100730 北京大学第五临床医学院北京100730

目的探讨在前列腺穿刺活检组织中应用CK5/6抗体快速免疫组化辅助冰冻病理诊断的准确性与可行性。方法回顾性分析2022年10月至2023年4月北京医院41例前列腺穿刺冰冻组织行CK5/6抗体快速免疫组化患者的资料。患者中位年龄76(69,79)岁,前... 详细信息

目的探讨在前列腺穿刺活检组织中应用CK5/6抗体快速免疫组化辅助冰冻病理诊断的准确性与可行性。方法回顾性分析2022年10月至2023年4月北京医院41例前列腺穿刺冰冻组织行CK5/6抗体快速免疫组化患者的资料。患者中位年龄76(69,79)岁,前列腺特异性抗原12.37(7.07,26.17)ng/ml。靶病灶的前列腺影像报告与数据系统(PI-RADS)评分均≥3分;其中3分9处(21.95%),4分15处(36.59%),5分17处(41.46%)。MRI检查病灶中位长径1.40(1.09,2.20)cm。病灶位于移行带14处(34.14%),外周带23处(56.10%),同时累及外周带和移行带病灶4处(9.76%)。采用经会阴认知融合靶向联合系统穿刺活检,术中于靶区和非靶区各额外取1针组织进行冰冻病理切片,并行HE染色和CK5/6抗体快速免疫组化染色,冰冻剩余组织行常规切片HE染色和CK5/6抗体常规免疫组化染色。记录冰冻病理和冰冻剩余组织常规切片的HE染色和免疫组化染色结果、国际泌尿病理学会(ISUP)分级分组,以及靶向联合系统穿刺的实际诊断结果等数据。以同一针穿刺组织的常规免疫组化结果为金标准,分析快速免疫组化冰冻病理诊断前列腺癌的敏感性和阳性预测值,及其病理分级分组的准确性。结果在41例患者中,靶区组织冰冻切片HE染色诊断前列腺癌35例,阳性率85.37%(35/41),其中ISUP分级分组1组17例(48.57%),2组8例(22.86%),3组4例(11.43%),4~5组6例(17.14%);同一针靶区冰冻剩余组织切片HE染色诊断前列腺癌35例,阳性率85.37%(35/41),其中ISUP分级分组1组16例(45.71%),2组9例(25.72%),3组4例(11.43%),4~5组6例(17.14%)。靶区组织快速免疫组化结果CK5/6表达阴性35例,阳性6例;常规免疫组化结果CK5/6阴性35例,阳性6例。非靶区组织快速免疫组化结果CK5/6表达阴性12例,阳性29例;常规免疫组化结果CK5/6阴性12例,阳性29例。靶区快速免疫组化诊断前列腺癌的35例与常规免疫组化诊断结果完全吻合,假阳性率为0,敏感性为100.00%(35/35),阳性预测值为100.00%(35/35)。非靶区快速免疫组化诊断前列腺癌的12例与常规免疫组化诊断结果完全吻合,假阳性率为0,敏感性为100.00%(35/35),阳性预测值为100.00%(12/12)。结论本研究结果初步证实,在前列腺穿刺活检组织中应用CK5/6抗体快速免疫组化辅助冰冻病理诊断,具有较高的准确性,但快速免疫组化技术在穿刺术中辅助冰冻病理诊断的可靠性仍需要大样本前瞻性研究进一步验证。

关键词：前列腺肿瘤癌前列腺穿刺活检冰冻病理快速免疫组化

Current and future molecular diagnostics in colorectal cancer and colorectal adenoma

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World Journal of Gastroenterology 2014年第14期20卷 3847-3857页

作者： Andy Hin-Fung Tsang Ka-Ho Cheng Apple Siu-Ping Wong Simon Siu-Man ng Brigette Buig-yue Ma Charles Ming-Lok Chan Nancy Bo-Yin Tsui Lawrence Wing-Chi Chan Benjamin Yat-Ming Yung Sze-Chuen Cesar Wong Department of Health Technology and Informatics Faculty of Health and Social SciencesHong Kong Polytechnic University Department of Surgery Prince of Wales Hospital State Key Laboratory in Oncology in South China Sir Y K Pao Centre for CancerDepartment of Clinical OncologyHong Kong Cancer Institute and Prince of Wales HospitalThe Chinese University of Hong Kong

Colorectal cancer(CRC)is one of the most prevalent cancers in developed *** the other hand,CRC is also one of the most curable cancers if it is detected in early stages through regular colonoscopy or *** CRC develops slowly from precancerous lesions,early detection can reduce both the incidence and mortality of the *** occult blood test is a widely used non-invasive screening tool for *** fecal occult blood test is simple and cost-effective in screening CRC,there is room for improvement in terms of the accuracy of the *** dysregulations have been found to play an important role in CRC *** better understanding of the molecular basis of CRC,there is a growing expectation on the development of diagnostic tests based on more sensitive and specific molecular markers and those tests may provide a breakthrough to the limitations of current screening tests for *** this review,the molecular basis of CRC development,the characteristics and applications of different non-invasive molecular biomarkers,as well as the technologies available for the detection were *** review intended to provide a summary on the current and future molecular diagnostics in CRC and its pre-malignant state,colorectal adenoma.

关键词： Colorectal cancer Colorectal adenoma Molecular diagnostics Fecal occult blood test Non-invasive

补肾养血膏方对女性卵巢功能减退血清FSH、LH、E_(2)、AMH及窦卵泡影响的研究

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中国现代药物应用 2024年第7期18卷 144-147页

作者：施燕飞施月春姚凤伟象山县中医医院医疗健康集团总院妇科 315700

目的探究与分析补肾养血膏方对女性卵巢功能减退的治疗效果。方法120例卵巢功能减退患者作为研究对象,根据治疗方式的不同分为对照组与治疗组,每组60例。对照组以胚宝胶囊治疗,治疗组提供补肾养血膏方治疗。对比两组临床疗效、血清性激... 详细信息

目的探究与分析补肾养血膏方对女性卵巢功能减退的治疗效果。方法120例卵巢功能减退患者作为研究对象,根据治疗方式的不同分为对照组与治疗组,每组60例。对照组以胚宝胶囊治疗,治疗组提供补肾养血膏方治疗。对比两组临床疗效、血清性激素[雌二醇(E_(2))、促卵泡生成素(FSH)、促黄体生成素(LH)]与抗苗勒管激素(AMH)水平、窦卵泡数、子宫内膜厚度、中医证候积分。结果治疗组临床治疗总有效率95.00%高于对照组的78.33%,具有明显差异(Png/ml高于对照组的(1.69±0.48)ng/ml,具有显著差异(P<0.05)。治疗后,治疗组子宫内膜厚度(8.74±1.04)mm与窦卵泡数(5.74±0.25)个均大于对照组的(6.58±1.29)mm、(4.50±0.31)个,具有显著差异(P<0.05)。治疗后,治疗组主要证候、次要证候、伴随证候及总证候积分分别为(7.29±2.14)、(2.87±1.27)、(1.09±0.14)、(11.25±3.55)分,低于对照组的(8.66±3.05)、(3.57±1.31)、(1.46±0.25)、(13.69±4.61)分,具有明显差异(P<0.05)。结论卵巢功能减退患者经补肾养血膏方治疗后,血清性激素、窦卵泡数均得到改善,且症状改善显著。

关键词：补肾养血膏方胚宝胶囊卵巢功能减退性激素窦卵泡

Increased serum levels of soluble CD146 and vascular endothelial growth factor receptor 2 in patients with exudative age-related macular degeneration

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International Journal of Ophthalmology(English edition) 2019年第3期12卷 457-463页

作者： Yan-Yao Liu yue Bin Xing Wang Hui Peng Department of Ophthalmology the First Affiliated Hospital of Chongqing Medical University Chongqing Key Laboratory of Ophthalmology Chongqing Eye Institute

AIM: To investigate serum levels of soluble CD146(s CD146) and vascular endothelial growth factor receptor 2(VEGFR2) in patients with age-related macular degeneration(AMD). METHODS: Eighty-eight patients with exudative AMD and 45 sex-and age-matched healthy controls were enrolled in this study conducted in China. Serum samples was obtained from the patients with exudative AMD and from the controls. Serum sCD146 and VEGFR2 protein levels were measured using an enzyme-linked immunosorbent ***: We found that serum sCD146 and VEGFR2 protein levels were significantly higher in the patients with exudative AMD group than in the controls(t=3.859, Png sCD146 levels(χ2=10.29, P=0.001). The receiver operating characteristic curve analysis showed that the area under the curve was 0.696 for s CD146(95%CI: 0.601-0.791) with an optimum diagnostic cut-off value of 157.16 ng/mL, a sensitivity of 55.7%, and a specificity of 82.2%.CONCLUSION: The serum sCD146 level increases and may be a biomarker for exudative AMD.

关键词： age-related macular degeneration soluble CD146 vascular endothelial growth factor receptor 2 serum