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npj Computational Materials 2020年第1期6卷 1376-1380页

作者： Xue-Peng Wang Xian-Bin Li Nian-Ke Chen junhyeok Bang Ryky nelson Christina Ertural Richard Dronskowski Hong-Bo Sun Shengbai Zhang State Key Laboratory of Integrated Optoelectronics College of Electronic Science and EngineeringJilin University130012 ChangchunChina Department of Physics Chungbuk National University28644 ChungbukRepublic of Korea Institute of Inorganic Chemistry RWTH Aachen University52056 AachenGermany Hoffmann Institute of Advanced Materials Shenzhen Polytechnic518055 ShenzhenChina State Key Lab of Precision Measurement Technology and Instruments Department of Precision Instrument Tsinghua Univerwsity100084 BeijingChina Department of Physics Applied Physicsand AstronomyRensselaer Polytechnic InstituteTroyNY 12180USA

Recently,all-optical memory and optical-computation properties of phase-change materials are receiving intensive *** writing/erasing information in these devices is usually achieved by laser pulses,the interaction between the laser and the phase-change materials becomes a key issue for such new *** this work,by a time-dependent density-functional theory molecular-dynamics study,the physics underlying the optical excitation induced amorphization of Sc-Sb-Te is revealed,which goes back to superatom-like Sc-centered structural *** motifs are found to be still robust under the excitation.

关键词： optical excitation theory

Insulin-like growth factor 1-induced enolase 2 deacetylation by HDAC3 promotes metastasis of pancreatic cancer

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Signal Transduction and Targeted Therapy 2020年第1期5卷 1857-1870页

作者： Yan Zheng Chao Wu jimeng Yang Yue Zhao Huliang jia Min Xue Da Xu Feng Yang Deliang Fu Chaoqun Wang Beiyuan Hu Ze Zhang Tianen Li Shican Yan Xuan Wang Peter j.nelson Christiane Bruns Lunxiu Qin Qiongzhu Dong Department of General Surgery Huashan Hospital&Cancer Metastasis Institute&Institutes of Biomedical SciencesFudan UniversityShanghai 200040China General Visceral and Cancer SurgeryUniversity Hospital of CologneCologne 50937Germany Department of Pancreatic Surgery Pancreatic Disease InstituteHuashan HospitalShanghai Medical CollegeFudan UniversityShanghaiChina Medical Clinic and Policlinic IV Ludwig-Maximilian-University(LMU)MunichGermany

Enolase 2(ENO2)is a key glycolytic enzyme in the metabolic process of glycolysis,but its potential function in pancreatic ductal adenocarcinoma(PDAC)is *** this study,we observed a significant overexpression of ENO2 in PDAC tissues,and its expression was correlated with metastasis and poor prognosis in PDAC patients.K394 was identified as a major acetylation site in ENO2 that regulates its enzymatic activity,cell metabolism and PDAC *** of ENO2 suppressed tumor growth and liver metastasis in ***-expression of wild-type(WT)ENO2,but not the K394 acetylation mimetic mutant,could reverse the decreased tumor *** further characterized histone deacetylase 3(HDAC3)and P300/CBP-associated factor(PCAF)as the potential deacetylase and acetyltransferase for ENO2,***3-mediated deacetylation was shown to lead to ENO2 activation and enhancement of ***,insulin-like growth factor-1(IGF-1)was found to decrease K394 acetylation and stimulate ENO2 activity in a dose-and time-dependent *** PI3K/AKT/mTOR pathway facilitated the phosphorylation of HDAC3 on S424,which promoted K394 deacetylation and activation of ***,an oral small-molecule inhibitor of IGF-1R,could inhibit IGF-1-induced ENO2 deacetylation by HDAC3 and the PI3K/AKT/mTOR ***,linsitinib showed a different effect on the growth and metastasis of PDAC depending on the overexpression of WT versus K394-mutant *** results reveal a novel mechanism by which acetylation negatively regulates ENO2 activity in the metastasis of PDAC by modulating *** of IGF-1-induced ENO2 deacetylation represents a promising strategy to prevent the development of PDAC.

关键词： HDAC3 metastasis metabolism

Engineering osteoblastic metastases to delineate the adaptive response of androgen-deprived prostate cancer in the bone metastatic microenvironment

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Bone Research 2019年第2期7卷 157-170页

作者： Nathalie Bock Ali Shokoohmand Thomas Kryza joan Rohl jonelle Meijer Phong A. Tran Colleen C. nelson judith A. Clements Dietmar W. Hutmacher School of Biomedical Sciences Faculty of Health and Australian Prostate Cancer Research Centre (APCRC-Q)Institute of Health and Biomedical Innovation (IHBI)Queensland University of Technology (QUT)BrisbaneQLD 4000Australia Translational Research Institute (TRI) WoolloongabbaQLD 4102Australia Centre in Regenerative Medicine QUTKelvin GroveQLD 4059Australia Bone and joint Disorders Program School of ChemistryPhysics and Mechanical EngineeringScience and Engineering Faculty (SEF)QUTBrisbaneQLD 4000Australia Australian Research Council (ARC) Training Centre in Additive Biomanufacturing QUTKelvin GroveQLD 4059Australia

While stromal interactions are essential in cancer adaptation to hormonal therapies,the effects of bone stroma and androgen deprivation on cancer progression in bone are poorly ***,we tissue-engineered and validated an in vitro microtissue model of osteoblastic bone metastases,and used it to study the effects of androgen deprivation in this *** model was established by culturing primary human osteoprogenitor cells on melt electrowritten polymer scaffolds,leading to a mineralized osteoblast-derived microtissue containing,in a 3D setting,viable osteoblastic cells,osteocytic cells,and appropriate expression of osteoblast/osteocyte-derived mRNA and proteins,and mineral *** co-culture of androgen receptordependent/ independent cell lines (LNCaP,C4-2B,and PC3) led cancer cells to display functional and molecular features as observed in ***-cultured cancer cells showed increased affinity to the microtissues,as a function of their bone metastatic *** led to alkaline phosphatase and collagen-I upregulation and sclerostin downregulation,consistent with the clinical marker profile of osteoblastic bone *** showed a significant adaptive response under androgen deprivation in the microtissues,with the notable appearance of neuroendocrine transdifferentiation features and increased expression of related markers (dopa decarboxylase,enolase 2).Androgen deprivation affected the biology of the metastatic microenvironment with stronger upregulation of androgen receptor,alkaline phosphatase,and dopa decarboxylase,as seen in the transition towards *** unique microtissues engineered here represent a substantial asset to determine the involvement of the human bone microenvironment in prostate cancer progression and response to a therapeutic context in this microenvironment.

关键词： Engineering osteoblastic metastases the adaptive response metastatic microenvironment

Expression of miR-15/107 Family MicroRNAs in Human Tissues and Cultured Rat Brain Cells

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Genomics, Proteomics & Bioinformatics 2014年第1期12卷 19-30页

作者： Wang-Xia Wang Robert j.Danaher Craig S.Miller joseph R.Berger Vega G.Nubia Bernard S.Wilfred janna H.Neltner Christopher M.Norris Peter T.nelson Sanders-Brown Center on Aging University of KentuckyLexingtonKY 40536USA College of Dentistry University of KentuckyLexingtonKY 40536USA Department of Neurology University of KentuckyLexingtonKY 40536USA Department of Pathology University of KentuckyLexingtonKY 40536USA Department of Molecular and Biomedical Pharmacology University of KentuckyLexingtonKY 40536USA

The miR-15/107 family comprises a group of 10 paralogous microRNAs （miRNAs）,sharing a 5＇ AGCAGC *** miRNAs have overlapping *** order to characterize the expression of miR-15/107 family miRNAs,we employed customized TaqMan Low-Density micro-fluid PCR-array to investigate the expression of miR-15/107 family members,and other selected miRNAs,in 11 human tissues obtained at autopsy including the cerebral cortex,frontal cortex,primary visual cortex,thalamus,heart,lung,liver,kidney,spleen,stomach and skeletal ***-103,miR-195 and miR-497 were expressed at similar levels across various tissues,whereas miR-107 is enriched in brain *** also examined the expression patterns of evolutionarily conserved miR-15/107 miRNAs in three distinct primary rat brain cell preparations （enriched for cortical neurons,astrocytes and microglia,respectively）.In primary cultures of rat brain cells,several members of the miR-15/107 family are enriched in neurons compared to other cell types in the central nervous system （CNS）.In addition to mature miRNAs,we also examined the expression of precursors （pri-miRNAs）.Our data suggested a generally poor correlation between the expression of mature miRNAs and their *** summary,we provide a detailed study of the tissue and cell type-specific expression profile of this highly expressed and phylogenetically conserved family of miRNA genes.

关键词： miR-16 miR-424 miR-503 miR-15 Normalization Target prediction

西藏高原上地幔的精细结构与构造——地震层析成像给出的启示

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地球物理学报 2004年第3期47卷 449-455页

作者：赵文津薛光琦吴珍汉赵逊刘葵史大年 j.Mechie D.nelson L.Brown T.Hearn 中国地质科学院中国地质科学院矿产资源研究所中国地质科学院地质力学研究所 GeoForchungs Zentrum(GFZ) Potsdam14473Germany Department of Earth Sciences Syracuse UniversitySyracuseNewYork13244USA Department of Geological Sciences Cornell UniversityIthacaNewYork14853USA Department of Physics New Mexico State UniversityLasCrucesNM88003USA

利用布置在亚东—格尔木的 1 6 4个流动地震台站记录的 92 6个远震事件的 2 4 2 4 1条射线 ,进行远震P波层析成像处理 ,高分辨率的西藏高原上地幔的速度结构图 ,显示了印度巨厚地幔岩石圈在向高原之下推进的过程中 ,在高喜马拉雅之下拆... 详细信息

利用布置在亚东—格尔木的 1 6 4个流动地震台站记录的 92 6个远震事件的 2 4 2 4 1条射线 ,进行远震P波层析成像处理 ,高分辨率的西藏高原上地幔的速度结构图 ,显示了印度巨厚地幔岩石圈在向高原之下推进的过程中 ,在高喜马拉雅之下拆分成上、下两层 ,这是发生的第一次拆沉 .下层从高喜马拉雅以下约以 2 2°的角度向高原北部插入到 35 0km深 ;而其上层则向北伸展直到雁石坪 ,并构成了高原薄的地幔岩石圈 .在雁石坪北 (33.7°N) ,当其与亚洲大陆岩石圈地幔相遇后发生断离并下沉 .再次证实了五道梁 (35 .2 7°N)深部低速体的存在 ,本区内地壳内低速物质可能与上述运动有联系。

关键词：西藏高原上地幔精细结构构造地震层析成像

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低噪声行波管放大器向固态器件挑战

真空电子技术 1972年第1期 30-37页

作者： j. N. nelson

目前有关低噪声微波放大器的文献,大多数是讨论固态器件的,而对低噪声行波管放大器的最近的革新发表的实际数据很少。这就会使人认为,全固态化办法是解决微波低噪声放大的最好途径。这种想法有一定道理,但是不能说低噪声行波管已经陈旧... 详细信息

目前有关低噪声微波放大器的文献,大多数是讨论固态器件的,而对低噪声行波管放大器的最近的革新发表的实际数据很少。这就会使人认为,全固态化办法是解决微波低噪声放大的最好途径。这种想法有一定道理,但是不能说低噪声行波管已经陈旧过时了。本文介绍低噪声行波管的现状,其主要优缺点,工作特性方面最近取得的一些进展以及行波管独特性能的典型应用。

关键词：低噪声行波管功率输出固态器件电子器件电子设备放大器噪声系数

兰迪·保施,身患绝症的教授用“最后一课”启迪千万人

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英语文摘 2008年第10期 17-21页

作者： Valerie j. nelson 王雷

被确诊患有胰腺癌后,他在卡内基·梅隆大学发表的演讲成了互联网上的奇观和畅销书。兰迪·保施于周五去世,终年47岁。这位身患绝症的教授在卡内基.梅隆大学发表的诚挚告别演说成了互联网上的奇观和畅销书,也使他成为善待生活与死亡的标... 详细信息

被确诊患有胰腺癌后,他在卡内基·梅隆大学发表的演讲成了互联网上的奇观和畅销书。兰迪·保施于周五去世,终年47岁。这位身患绝症的教授在卡内基.梅隆大学发表的诚挚告别演说成了互联网上的奇观和畅销书,也使他成为善待生活与死亡的标志性人物。

关键词：卡内基梅隆大学最后一课世界百科全书

If this is true, what does it imply? How end-user antibody validation facilitates insights into biology and disease

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Asian journal of Urology 2019年第1期6卷 10-25页

作者： Karen S.Sfanos Srinivasan Yegnasubramanian William G.nelson Tamara L.Lotan Ibrahim Kulac jessica L.Hicks Qizhi Zheng Charles j.Bieberich Michael C.Haffner Angelo M.De Marzo Department of Pathology Johns Hopkins University School of MedicineBaltimoreMDUSA Department of Oncology The Johns Hopkins University School of MedicineBaltimoreMDUSA Department of Pathology Koc Universitesi Tip FakultesiIstanbulTurkey Department of Biological Sciences University of Maryland Baltimore CountyBaltimoreMDUSA

Antibodies are employed ubiquitously in biomedical sciences,including for diagnostics and *** of the most important uses is for immunohistochemical(IHC)staining,a process that has been improving and evolving over *** is useful when properly employed,yet misuse of the method is widespread and contributes to the“reproducibility crisis”in *** report some of the common problems encountered with IHC assays,and direct readers to a wealth of literature documenting and providing some solutions to this *** also describe a series of vignettes that include our approach to analytical validation of antibodies and IHC assays that have facilitated a number of biological insights into prostate cancer and the refutation of a controversial association of a viral etiology in *** postulate that a great deal of the problem with lack of accuracy in IHC assays stems from the lack of awareness by researchers for the critical necessity for end-users to validate IHC antibodies and assays in their laboratories,regardless of manufacturer claims or past *** suggest that one reason for the pervasive lack of end-user validation for research antibodies is that researchers fail to realize that there are two general classes of antibodies employed in ***,there are antibodies that are“clinical grade”reagents used by pathologists to help render diagnoses that influence patient *** diagnostic antibodies,which tend to be highly validated prior to clinical implementation,are in the vast minority(e.g.3800000),which are often not extensively validated prior to *** increased awareness of the problem,both the United States,National Institutes of Health and some journals are requiring investigators to provide evidence of specificity of their antibody-based assays.

关键词： Prostate cancer Antibodies Immunohistochemistry