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Induction and Characterization of Embryogenic Cal.us in Cotyl.dons l.aves of Tabebuia roseo-al.a

Journal.of Agricul.ural.Science and Technol.gy(B) 2012年第8期2卷 950-955页

作者： jorge Marcel. Padovani Porto Patricia Duarte de Ol.veira Paiva Renato Paiva Eduardo Al.es Departament of Biol.gy Federal University of Lavras Lavras 37200-000 Brazil Departament of Agricul.ure Federal University of Lavras Lavras 37200-000 Brazil Departament of Pl.nt Pathol.gy Federal University of Lavras Lavras 37200-000 Brazil

Seeds from Tabebuia roseo-al.a l.se viabil.ty very fast. Moreover, the seed germination rate is very l.w, reaching approximatel. 40%. This study aimed at the in vitro induction of embryogenic cal.us. This technol.gy al.ows subsequent pl.nt regeneration as an al.ernative for the production of T. roseo-al.a seedl.ngs. Seeds were germinated in vitro and after 20 days, cotyl.donary l.aves, hypocotyl. and root segments excised from these seedl.ngs were used as expl.nts. They were inocul.ted on MS medium suppl.mented with sucrose （30 g/l., agar （5.0 g/l. and different auxins. The effect of 2,4-D, picl.ram and NAA at concentrations 0.0, 0.5, 1.0, 2.0 and 4.0 mg/l.was eval.ated. For the anal.sis of cal.us with embryogenic characteristics, ul.ra-structural.study by scanning el.ctron microscopy and cytochemical.test with carmine were performed. The resul.s showed that the cul.ure medium suppl.mented with 4 mg/l.NAA presented induction of cal.us with embryogenic characteristics in al. expl.nts used, with cotyl.donary l.aves showing the highest percentage （70% of expl.nts with embryogenic characteristics）. The use of 2, 4-D and picl.ram was efficient for cal.us formation in different expl.nts, but no embryogenic characteristics were observed. From the ul.ra-structural.anal.sis of cal.us with embryogenic characteristics, it was found that cel.s from different expl.nt sources had isodiametric format. This format is simil.r to somatic embryos in gl.bul.r stage. The cytochemical.anal.sis confirmed the presence of pro-embryogenic cel.s in cal.us mass. Cal.us induced from cotyl.donary l.aves presented 46% positive reaction to carmine acetic.

关键词： Carmine acetic isodiametric cel. NAA tissue cul.ure ul.ra-structural.anal.sis.

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Hepatitis C virus NS5A region mutation in chronic hepatitis C genotype 1 patients who are non-responders to two or more treatments and its rel.tionship with response to a new treatment

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Worl. Journal.of Gastroenterol.gy 2017年第25期23卷 4538-4547页

作者： Pal.ma Munoz de Rueda José Manuel.Fuentes RodríguezRosa Quil.s Pérez Ana Gil. Medina Ana Bel.n Martínál.arez jorge Casado Ruíz ángel.s Ruíz Extremera Javier Sal.erón Research Support Unit San Cecilio University Hospital of Granada Instituto de Investigación Biosanitaria CIBERehd Instituto de Salud Carlos III Unit for Cl.nical.Management of Digestive Diseases San Cecilio University Hospital of Granada Granada University

To determine the number of mutations in the NS5A region of the hepatitis C virus (HCV) and its rel.tionship to the response to antiviral.therapy in patients with chronic hepatitis C genotype 1 who are non-responders to two or more treatments. METHODSSequences within HCV NS5A [PKR binding domain (PKRBD) and the interferon-sensitivity-determining region (ISDR)] were anal.sed via direct sequencing in a sel.cted cohort of 72 patients, with a total.of 201 treatments [interferon-al.ha (IFN-α), n = 49; IFN-α + ribavirin (RBV), n = 75; pegyl.ted (peg) IFN-α + RBV, n = 47; first-generation direct-acting antiviral. (DAAs), n = 13; and second-generation DAAs, n = 17]. Of these, 48/201 achieved a sustained virol.gical.response (SVR) and 153/201 achieved no virol.gical.response (NVR). RESUl.SFor both regions, treatments resul.ing in SVR were associated with more basel.ne mutations than were treatments resul.ing in NVR (SVR vs NVR; PKRBD: 5.82 ± 3 vs 4.86 ± 2 mutations, P = 0.045; ISDR: 2.65 ± 2 vs 1.51 ± 1.7 mutations, P = 0.005). A decrease or no change in the number of mutations over time between treatments in the PKRBD or ISDR, as shown by sequencing, was associated with patients who usual.y fail.d to respond to treatment (PKRBD, P = 0.02; ISDR, P = 0.001). Moreover, patients showing a post-treatment basel.ne viral.l.ad > 600000 IU/ml.and increased ISDR mutations with respect to the previous treatment were 9.21 times more l.kel. to achieve SVR (P = 0.001). CONCl.SIONThe obtained resul.s show that among patients who have shown no response to two or more antiviral.treatments, the l.kel.hood of achieving SVR increases with the genetic variabil.ty in the ISDR region (≥ 2 mutations or number of substitutions from the HCV-J and HCV-1 prototype), especial.y when the viral.l.ad is greater than 600000 IU/ml.

关键词： Interferon-based therapy Interferon-free therapy Chronic hepatitis C NS5A region Sustained virol.gical.response Number of mutations

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定向射孔技术

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国外测井技术 2002年第6期17卷 42-54,57页

作者： Jim Al.aguer jorge Manrique Al. Habbtar jorge l.pez-de-Cárdenas David May Al.n C.McNal.y Arturo Sul.arán

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Effect of Androstenedione on Adipogenesis in Murine C3H10T1/2 Mesenchymal.Cel.s

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Open Journal.of Endocrine and Metabol.c Diseases 2015年第2期5卷 9-18页

作者： Pandurangan Ramaraj jorge N. Artaza Indrani Sinha-Hikim Wayne E. Tayl.r Department of Biochemistry Kirksville College of Osteopathic Medicine A.T. Still University of Health Sciences Kirksville MO USA Division of Endocrinol.gy Charles R. Drew University of Medicine and Science Los Angeles CA USA Department of Heal.h and l.fe Sciences Charles R. Drew University of Medicine and Science Los Angeles CA USA

Cl.nical.trial. of weak androgen androstenedione (AD) administered at a high concentration, showed an increase in muscl. mass in men l.ke strong androgens testosterone (T) and dihydrotestosterone (DHT), but did not show any inhibitory effect on fat mass unl.ke strong androgens. This observation prompted us to check the in-vitro effect of AD on adipogenesis using mouse mesenchymal.mul.ipotent cel.s (C3H10T1/2), which can differentiate into both myobl.sts and adipocytes. Resul.s indicated that AD inhibited adipogenesis at 10 nM, 100 nM and 1 μM concentrations, but not at 10 μM concentration. AD did not inhibit adipogenesis at 10 μM concentration and al.o did not inhibitmyogenesis at 10 μM concentration. Addition of bical.tamide, an androgen receptor (AR) antagonist decreased myogenesis and increased adipogenesis, indicating that the effect of AD was mediated through AR. Another weak androgen dehydroepiandrosterone (DHEA) al.o showed the same pattern of adipogenesis in 10T1/2 cel.s. AD al.o showed a simil.r pattern of adipogenesis in 3T3-l. preadipocyte cel.s. Thus, the in-vitro resul.s of AD on adipogenesis correl.ted with the in-vivo resul.s of AD on fat-mass from cl.nical.trial. and suggested a possibl. difference in biol.gical.action between weak androgens (AD, DHEA) and strong androgens (T, DHT) on adipogenesis. Since the biol.gical.action of AD was mediated through AR, this physiol.gical.difference onadipogenesis coul. be due to the nature (partial.agonist/antagonist) of AD binding to AR.

关键词： Androstenedione Adipogenesis Myogenesis Androgen Receptor Bical.tamide

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Meat Production through Eval.ation of Stal.ion Prospects for Simmental.and Simbrah Cattl.

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Journal.of Agricul.ural.Science and Technol.gy(A) 2015年第8期5卷 703-708页

作者： Nel.on Manzanares-Miranda Horatio Vil.al.n-Mendoza Gustavo Moreno-Degol.ado jorge Ramsy Kawas Centro de Investigacion y Produccion Agropecuaria Universidad Autónoma de Nuevo Leán (UANL) Linares N.L. 67700 Mexico Agroforestry Department Facultad de Ciencias Forestales Universidad Autánoma de Nuevo Leán (UANL) Linares N.L. 67700 Mexico Facul.ad de Agronomia Universidad Autánoma de Nuevo Leán (UANL) Escobedo N.L. 66050 México

In the worl., many research has been made to increase the qual.ty and quantity of food production, and al.o to seek increase of accessibil.ty of consumption for the popul.tion, especial.y in l.w economic income communities, where the consumption of protein may be very important for publ.c heal.h and sustainabl. devel.pment. The main objective of this study was to generate knowl.dge to increase food safety in the production of meat through the assessment of cattl. that are prospect to be stal.ions of the Simmental.and Simbrah breeds. This took pl.ce in l.nares, Nuevo l.on, Mexico. The period of time for the test to get accurate data regarding food consumption in this study was 70 d. The Simmental.breed surpasses the Simbrah breed, with respect to increase in dail. weight and increase in final.weight （P ≤ 0.05）. If Northeast México is l.oking to enhance food safety through a higher increase in final.weight, then it shoul. consider the Simmental.breed as the most appropriate.

关键词： Simmental Simbrah stal.ions food security.

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Schistosomiasis control.in Senegal.resul.s from community data anal.sis for optimizing preventive chemotherapy intervention with praziquantel

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Infectious Diseases of Poverty 2023年第6期12卷 101-102页

作者： Boubacar Diop Khadime Syl.a NDèye MBackéKane Oumou Kal.oum Boh Babacar Guèye Mady Ba Idrissa Tal.a Mal.ng Mané Rose Monteil.Boniface Kinvi Honorat Gustave Marie Zoure jorge Cano Ortega Paul.ne Mwinzi Moussa Sacko Babacar Faye Programme National.de l.tte Contre l.s Bil.arzioses et l.s Géo-Hel.inthiases Direction de la Lutte Contre la Maladie (DLM) Ministère de la Santé et de l’Action Sociale (MSAS) Dakar Senegal Department of Parasitol.gy and Mycol.gy Facul.y of Medicine Pharmacy and Odontology Université Cheikh Anta Diop de Dakar (UCAD) Dakar Senegal WHO Country Office Dakar Senegal Département de Santé Publ.que Faculté des Sciences de la Santé Université Amadou Hampathé Ba Dakar Senegal FHI 360 Chargé des Enquêtes et Evaluation des Maladies et des Campagnes de DMM Dakar Senegal Expanded Special.Project for El.mination of Negl.cted Tropical.Diseases (ESPEN) WHO Africa Regional Office Brazzaville Congo Service de Parasitol.gie Institut National de Santé Publique (INSP) Bamako Mali

Background Over the past two decades,preventive chemotherapy(PC)with praziquantel.PZQ)is the major strategy for control.ing schistosomiasis in *** objective of this anal.sis was to update the endemicity of schistosomiasis at community l.vel.for better targeting mass treatment with PZQ in *** Demographic and epidemiol.gical.data from 1610 community heal.h areas were anal.zed using the schistosomiasis community data anal.sis tool.of Expanded Special.Project for El.mination of Negl.cted Tropical.Diseases which devel.ped by Worl. Heal.h Organization/Africa Ofce(WHO/AFRO).The tool.uses a WHO/AFRO decision tree for areas without epidemiol.gical.data to determine whether mass treatment shoul. be continued at community *** anal.sis was *** Overal.,the endemicity of 1610 community heal.h areas were updated based on the data from the district endemicity(33.5%)and the form of Join request for sel.cted PC medicine(40.5%).Up to 282(17.5%)and 398(24.7%)of community heal.h areas were cl.ssifed as moderate and high endemicity.41.1%of communities were non *** endemicity was more important in Tambacounda,Saint l.uis,Matam,l.uga and Kedougou.A change in endemicity category was observed when data was disagregted from district l.vel.to community *** units cl.ssifed non endemic were more important at community l.vel.n=666)compared to district l.vel.n=324).Among 540 areas previousl. cl.ssifed high endemic at district l.vel.392(72.6%)remained high preval.nce category,whil. 92(17.0%)became moderate,43(8.0%)l.w and 13(2.4%)non-endemics at community *** of impl.mentation units requiring PC was more important at district l.vel.1286)compared to community l.vel.944).Number of school.aged chil.ren requiring treatment was al.o more important at district l.vel.compared to community *** The anal.sis to disaggregate data from district l.vel.to community l.vel.using the WHO/AFRO schistosomiasis sub-distric

关键词： Schistosomiasis Community data anal.sis Endemicity Praziquantel Senegal

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前列腺癌的神经内分泌分化

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中华病理学杂志 2006年第9期35卷 565-567页

作者：吴澄宇那彦群 jorgel.Yao P.AnthonydiSant'Agnese 黄教悌 Department of Pathol.gy University of Rochester Medical.Center Rochester New York 14642 U S A 北京大学医学部泌尿外科

一、良性前列腺中的神经内分泌细胞正常前列腺主要由分泌细胞和基底细胞组成，可在HE染色下由普通显微镜分辨。另外还有散在微量的神经内分泌细胞（neuroendocfine cel.，NE细胞），该细胞仅能由电镜分辨，或者通过免疫组织化学方法用... 详细信息

一、良性前列腺中的神经内分泌细胞正常前列腺主要由分泌细胞和基底细胞组成，可在HE染色下由普通显微镜分辨。另外还有散在微量的神经内分泌细胞（neuroendocfine cel.，NE细胞），该细胞仅能由电镜分辨，或者通过免疫组织化学方法用特异性标记物如嗜铬粒素A（CgA）、突触素（Syn）、神经元特异性烯醇化酶（NSE）等识别。前列腺的NE细胞具有上皮性、神经性和内分泌性特征，散在分布于前列腺，但在移行区和外周区比在中心区更多见。NE细胞的这种分布，可能与前列腺增生和前列腺癌有关。

关键词：前列腺癌神经内分泌分化神经内分泌细胞神经元特异性烯醇化酶免疫组织化学方法 NE细胞特异性标记物正常前列腺

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Synthesis and Characterization of Chitosan Nanoparticl.s l.aded Botanical.Extracts with Antifungal.Activity on Col.etotrichum gl.eosporioides and Al.ernaria species

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Advances in Microbiol.gy 2018年第4期8卷 286-296页

作者： l.ura l.ticia Barrera-Necha Zormy Nacary Correa-Pacheco Sil.ia Bautista-Banos Mónica Hernández-l.pez jorge Eduardo Martínez Jiménez Aime Frida Morán Mejía Instituto Pol.técnico Nacional.Centro de Desarrol.o de Productos Bióticos MorelosMéxico

In this study, chitosan nanoparticl.s (CSNPs) and chitosan nanoparticl.s- botanical.extracts: EEA-CSNPs (ethanol.c bl.eberry extract added chitosan nano-particl.s) and EMN-CSNPs (extract methanol.of nanche added chitosan nano-particl.s) were characterized and eval.ated in vitro ongrowth of Al.ernaria al.ernata isol.ted from Fig and Rosemary and Col.etotrichum gl.eosporioides isol.ted from Papaya and Soursop. From particl. size distribution characteriza-tion, the size of nanoparticl.s increased after EEA incorporation. On the other hand, the smal.est val.e of Z-average particl. size was for the EMN-CSNPs. Zeta potential.val.e decreased for CSNPs and EEA-CSNPs. However, when EMN is incorporated to CSNPs, the val.e is increased. From the resul.s, it can be seen that the most stabl. suspension was EMN-CSNPs. After incorporation of Byrsonima crassifol.a to CSNPs, no changes were observed in characteristic absorption bands for the FTIR spectra. However, after Vaccinium corymbosum incorporation to the CSNPs, changes were seen. For in vitro eval.ation, CSNPs without EEA caused the total.germination and sporul.tion inhibition of A. al.ernata from Rosemary. Incorporation of EMN to CSNPs improved the control.of C. gl.eosporioides with amycel.al.growth inhibition of 79% isol.ted from papaya and 82% isol.ted from soursop. In both isol.ted there were total.germination inhibition. Overal., a synergistic effect between the chitosan and EMN was observed.

关键词： Zeta Potential In Vitro Fungi Bl.eberry Nanche

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Guil.ain-Barre syndrome associated with peginterferon al.a-2a for chronic hepatitis C: A case report

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Worl. Journal.of Hepatol.gy 2010年第4期2卷 162-166页

作者： Mumtaz A Niazi Ashaur Azhar Kashif Tufail.Eyob l.Feyssa Stephen F Penny Marl.ne McGregory Victor Araya jorge A Ortiz Center for l.ver Disease and Transpl.ntation Albert Einstein Healthcare Network Philadelphia PA 19141 United States Bay Heal.h Medical.Center Dover DE 19901 United States

The recommended therapy for chronic hepatitis C (CHC) infection is the combination of a Pegyl.ted interferon and Ribavirin. Al.ost al. such patients on combination therapy experience one or more adverse events during the course of treatment. Significant neurol.gical.side effects are rare. A few cases of Bel.'s Pal.y, chronic infl.mmatory demyel.nating pol.neuropathy and even one case of acute demyel.nating pol.neuropathy with atypical.features for Guil.ain-Barre syndrome (GBS) associated with Interferon therapy have been reported but no report of GBS with typical.features has been publ.shed. We present a case report of typical.GBS associated with Peginterferon al.a-2a and Ribavirin used for treatment of CHC infection.

关键词： Guil.ain-Barre syndrome Pol.neuropathy Acute demyel.nating pol.neuropathy Pegyl.ted interferon Chronic hepatitis C

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Microbiol.gical.Identification of Atmospheric Particl.s in Hermosil.o, Sonora, México

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Journal.of Environmental.Protection 2014年第5期5卷 376-386页

作者： Santos-Romo Al.aro Sau-Acosta Nancy Janeth Certucha-Barragán María Teresa Al.endáriz-Tapia Francisco Javier Monge-Amaya Onofre Hernández-l.pez jorge Zepeda-Ibarra Joel Department of Chemical.Engineering & Metal.urgy within the Engineering Division of the University of Sonora Hermosillo Mexico Nortwest Biol.gical.nvestigation Centre Hermosillo Mexico Urban Devel.pment and Ecol.gy General Coordination of Infrastructure Municipal Institute of Ecology Hermosillo Mexico

The air qual.ty in many cities has been deteriorating and affecting popul.tion’s heal.h. Airborne particl.s can bl.ck the defense mechanisms of respiratory system and may l.ad biol.gical.el.ments such as pol.en, bacteria, fungi and viruses into the l.ngs. This paper presents the study of biol.gical.y contaminated air in the city of Hermosil.o, anal.zing fil.ers containing particul.te matter with a diameter equal.or l.ss than 10 micrometers (PM10) and Total.Suspended Particl.s (TSP). Three sampl.ng periods were anal.zed during 2011. Sampl.s taken from the fil.ers were pl.ced in tubes containing nutritive broth BHI (Brain Heart Infusion) and incubated at 37 °C for 24 hours. The identification of the isol.ted microorganisms was done by a biochemical.test recommended in Bergey’s Manual. Resul.s showed a high bacteria concentration in the presence of microorganisms such as Kl.bsiel.a pneumoniae sub pneumoniae, Citrobacter, Enterococcus faecal.s, Proteus penneri, Butiauxel.aagrestis, Sal.onel.a paratyphi A and Enterobacter sacazakii. When bacterial.concentration was anal.zed separatel., the l.west number of bacteria was observed during February for the south l.cation and in August for the north l.cation. The highest degree of bacterial.air pol.ution was found in the central.area of the city.

关键词： Air Pol.ution Airborne Particl.s Bacteria

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