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Circulating tumor and cancer stem cells in hepatitis C virusassociated liver disease

World Journal of Gastroenterology 2014年第48期20卷 18240-18248页

作者： Abeer A Bahnassy Abdel-Rahman N Zekri Ahmed El-Bastawisy Amal Fawzy Marwa Shetta nehal hussein Dalia Omran Abdallah A S Ahmed Samir S El-Labbody Molecular Pathology Unit Pathology Department National Cancer Institute Cairo University Cairo 11796 Egypt Virology and Immunology Unit Cancer Biology Department National Cancer Institute Cairo University Cairo 11796 Egypt Medical Oncology Department National Cancer Institute Cairo University Cairo 11796 Egypt Clinical Pathology Department National Cancer Institute Cairo University Cairo 11796 Egypt Clinical Pathology Kasr Al-Aini Cairo university Cairo 11782 Egypt Tropical Medicine Department Faculty of Medicine Cairo University Cairo 11796 Egypt Microbiology Department El-Azhar University Cairo 11964 Egypt

AIM:To assess the role of circulating tumor cells(CTCs)and cancer stem cells(CSCs)in hepatitis C virus(HCV)-associated liver ***:Blood and/or tissue samples were obtained from HCV(genotype 4)-associated hepatocellularcarcinoma patients(HCC;n=120),chronic hepatitis C patients(CH;n=30)and 33 normal control subjects(n=33).Serum levels of alpha-fetoprotein(AFP),alkaline phosphatase,and alanine and aspartate aminotransferases were *** 19(CK19)monoclonal antibody was used to enumerate CTCs,and CD133 and CD90 were used to enumerate CSCs by flow *** expression levels of the CSCs markers(CD133 and CD90)as well as telomerase,melanoma antigen encoding gene 1(MAGE1)and MAGE3 were assessed by RTPCR and quantitative real-time polymerase chain *** number of CTCs and/or the expression levels of CK19,CD133,telomerase,MAGE1 and MAGE3 were correlated to the standard clinicopathologic prognostic factors and disease ***:Levels of AFP,alkaline phosphatase and aspartate aminotransferase were significantly different among the HCC,CH and control groups(P<0.001),whereas alanine aminotransferase differed significantly between patient(HCC and CH)and control groups(P<0.001).At the specified cutoff values determine by flow cytometry,CK19(49.8),CD90(400)and CD133(73)were significantly higher in the blood of HCC patients compared to those in the CH and control groups(P<0.001).On the other hand,CD133 at a 69.5 cutoff was significantly higher in the CH compared to the control group(P≤0.001).Telomerase,MAGE1 and MAGE3RNA were expressed in 55.71%,60.00%and 62.86%of the HCC patients,respectively,but were not detected in patients in the CH or control groups,which were statistically significant(Ps<0.001).The expression levels of telomerase,CD90,MAGE3,CD133 and CK19 were all significantly associated with high tumor grade and advanced stage in HCC patients(all P s<0.05).CONCLUSION:CTC counts and AFP,CK19,telomerase,and MAGE1/MAGE3 expression

关键词： Cancer stem cells Circulating tumor cells Hepatiti

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