检索结果-南通市图书馆

Spectroscopic evidence for triplet excitation energy transfer among carotenoids in the LH2.complex from photosynthetic bacterium Rhodopseudomonas palustris

引用

Science china Chemistry 2004年第1期47卷 80-90页

作者： FENG Juan, WANG Qian, ZHANG Xujia, HUANG Youguo, Al Xicheng, ZHANG Xingkang & ZHANG JianpingState Key laboratory for Structural Chemistry of Unstable and Stable Species, institute of Chemistry, Chinese Academy of Sciences,{2. 100080, china national laboratory of biomacromolecules, institute of biophysics, Chinese Academy of Sciences, beijing 100101, china State Key laboratory for Structural Chemistry of Unstable and Stable Species institute of Chemistry Chinese Academy of Sciences Beijing China national laboratory of biomacromolecules institute of biophysics Chinese Academy of Sciences Beijing China

The LH2.complex from Rhodopsudomonas (Rps.) palustris is unique in the heterogeneous carotenoid compositions. The dynamics of triplet excited state Carotenoids (3Car* has been investigated by means of sub-microsecond time-resolved absorption spectroscopy both at physiological temperature (2.5 K) and at cryogenic temperature (77K). Broad and asymmetric T n ←T 1 transient absorption was observed at room temperature following the photo-excitation of Car at 532.nm, which suggests the contribution from various carotenoid compositions having different numbers of conjugated C=C double bonds (Nc=c). The triplet absorption bands of different carotenoids, which superimposed at room temperature, could be clearly distinguished upon decreasing the temperature down to 77 K. At room temperature the shorter-wavelength side of the main Tn04T1 absorption band decayed rapidly to reach a spectral equilibration with a characteristic time constant of ∽1 μs, the same spectral dynamics, however, was not observed at 77 K. The aforementioned spectral dynamics can be explained in terms of the triplet-excitation transfer among heterogeneous carotenoid compositions. Global spectral analysis was applied to the time-resolved spectra at room temperature, which revealed two spectral components peaked at 545 and 565 nm, and assignable to the Tn04 T1 absorption of Cars with Nc=c=11 and Nc=c=13, respectively. Surprisingly, the decay time constant of a shorter-conjugated Car, i.e. 0.72.?s (aerobic) and 1.36 ?s (anaerobic), is smaller than that of a longer-conjugated Car, i.e. 2.12.us (aerobic) and 3.75 ?s (anaerobic), which is contradictory to the general rule of carotenoids and relative polyenes. The results are explained in terms of triplet-excitation transfer among different types of Cars. It is postulated that two Cars with different conjugation lengths coexist in an α, β-subunit in the LH2.complex.

关键词： purple photosynthetic bacteria, carotenoid, excited-state, time-resolved spectroscopy.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Crystallization and preliminary X-ray studies of porcine kidney aminoacylase Ⅰ

引用

Chinese Science Bulletin 1997年第20期42卷 1750-1752页

作者： ZHAOHaiyan, MENGWuyi, LIN Zhengjiong and ZHOU Haimeng1. national laboratory of biomacromolecules, institute of biophysics, Chinese Academy of Sciences,{2., china 2. Department of Biological Science and Biotechnology, Tsinghua University, beijing 100084, china national laboratory of biomacromolecules Institute of Biophysics Chinese Academy of Sciences Beijing China Department of Biological Science and Biotechnology Tsinghua University Beijing China

AMINOACYLASE Ⅰ（ACY-1）（E. C. 3. 5. 1. 14 ） participated in amino acid metabolism existingin mammalian kidney and microorganisms, and catalyzes reversible hydrolysis of acylaminoacids. ACY-1 consists of 772.amino acids, and the molecular weight is 85 500 daltons. It is adimeric metalloprotein having two Zn2. in the molecule. The nucleotide and the amino acid se-quence of porcine and human ACY-1 have been determined. The nucleotide sequence and

关键词： aminoacylase I crystallization preliminary crystallography.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Further study on Ca_(2.) -mediated inhibition of human erythrocyte D-glucose transporter

引用

Chinese Science Bulletin 1997年第10期42卷 871-875页

作者： XIE Wensheng, TU Yaping and YANG FuyuState Key laboratory of biomacromolecules,{2., Chinese Academy of Sciences, beijing 100101, china State Key laboratory of biomacromolecules Institute of Biophysics Chinese Academy of Sciences Beijing China

THE transport of D-glucose to erythrocytes is catalyzed by an integral membrane protein,*** D-glucose *** specifically carries D-glucose,driven by glucose’s *** of its relative abundance and higher transport activity... 详细信息

关键词： Ca2. glucose transporter transporting activity inhibition.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Silence of Synaptotagmin VII inhibits release of dense core vesicles in PC12.cells

引用

Science china(Life Sciences) 2009年第12期52卷 1156-1163页

作者： LI JiangLi1, XIAO Yang1, ZHOU Wei1, WU ZhengXing1, ZHANG RongYing1· & XU Tao1,2. 1 Key laboratory of Molecular biophysics of the Ministry of Education,{2. and Biochemistry, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, china 2.national Key laboratory of biomacromolecules,{2., Chinese Academy of Sciences, beijing 100101, china. Key laboratory of Molecular biophysics of the Ministry of Education Institute of Biophysics and Biochemistry College of Life Science and Technology Huazhong University of Science and Technology Wuhan China national Key laboratory of{2. Institute of Biophysics Chinese Academy of Sciences Beijing China

Synaptotagmin VII (Syt VII), which has a higher Ca2. affinity and slower disassembly kinetics with lipid than Syt I and Syt IX, was regarded as being uninvolved in synaptic vesicle (SV) exocytosis but instead possibly as a calcium sensor for the slower kinetic phase of dense core vesicles (DCVs) release. By using high temporal resolution capacitance and amperometry measurements, it was demonstrated that the knockdown of endogenous Syt VII attenuated the fusion of DCV with the plasma membrane, reduced the amplitude of the exocytotic burst of the Ca2.-triggered DCV release without affecting the slope of the sustained component, and blocked the fusion pore expansion. This suggests that Syt VII is the Ca2. sensor of DCV fusion machinery and is an essential factor for the establishment and maintenance of the pool size of releasable DCVs in PC12.cells.

关键词： Synaptotagmin VII dense core vesicles exocytosis RNA-mediated interference (RNAi)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

TRAF-mediated regulation of immune and inflammatory responses

引用

Science china(Life Sciences) 2010年第2期53卷 159-168页

作者： WANG YaYa1,ZHANG Peng2.LIU YingFang2.& CHENG GenHong1 1 Department of Microbiology,Immunology & Molecular Genetics,University of California Los Angeles,609 Charles E.Young Drive East,Los Angeles,California 90095,USA 2.national laboratory of biomacromolecules,institute of{2.,Chinese Academy of Sciences,beijing 100101,china Department of Microbiology Immunology & Molecular Genetics University of California Los Angeles Los Angeles USA national laboratory of biomacromolecules Institute of Biophysics Chinese Academy of Sciences Beijing China

The tumor necrosis factor (TNF) receptor-associated factor (TRAF) family consists of six mammalian members,and is shown to participate in signal transduction of a large number of receptor families including TNF receptor family (TNFR) and Toll-like receptors-interleukin-1 receptors (TLR-IL-1R) *** receptor activation,TRAFs are directly or indirectly recruited to the intracellular domains of these *** subsequently engage other signaling proteins to activate inhibitor of κB kinase (IKK) complex,TRAF family member-associated NF-κB activator (TANK)-binding kinase 1 (TBK1) and inducible I κB kinase (IKK-i) (also known as IKKε),ultimately leading to activation of transcription factors such as NF-κB and interferon-regulatory factor (IRF) to induce immune and inflammatory responses.

关键词： TRAF NF-κB type 1 interferon innate immunity

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Alpha-latrotoxin Triggers Extracellular Ca2.-dependent Exocytosis and Sensitizes Fusion Machinery in Endocrine Cells

引用

Acta Biochimica et Biophysica Sinica 2006年第1期38卷 8-14页

作者： Zhi-Tao HU~(1#),Ping ZHAO~(1#),Jie LIU~(1#),Zheng-Xing WU~(1*),and Tao XU~(1,2.) ~1 School of Life Science and Technology,Huazhong University of Science and Technology,Wuhan 430074,china ~2.national laboratory of biomacromolecules,{2. of biophysics,Chinese Academy of Sciences,beijing 100101,china School of Life Science and Technology Huazhong University of Science and Technology Wuhan 430074 China national laboratory of biomacromolecules Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China

α-Latrotoxin from the venom of black widow spider induces and augments neurotransmitterand hormone release by way of extracellular Ca2.influx and cellular signal transduction *** usingwhole cell current and capacitance recording,the photolysis of card Ca2.,and Ca2.microfluorometry andamperometry,we investigated the stimulating effect and mechá（?）ism of α-latrotoxin on exocytosis in ratpancreatic β cells,LβT2.cells and latrophilin plasmid-transfected INS-1 *** data indicated that:（1）α-latrotoxin increased cytosolic Ca2.concentration through the formation of cation-permitting pores and sub-sequent Ca2.influx with the presence of extracellular Ca2.;（2.α-latrotoxin stimulated exocytosis in normalbath solution and its stimulating effect on secretion was eradicated in Ca2.-free bath solution; and （3）α-latrotoxin sensitized the molecular machinery of fusion through activation of protein kinase C and increasedthe response of cells to Ca2.photolysed by a flash of ultraviolet *** summary,α-latrotoxin inducedexocytosis by way of Ca2.influx and accelerated vesicle fusion by the sensitization of fusion machinery.

关键词： α-latrotoxin exocytosis calcium Ca2.-sensitivity of fusion protein kinase C(PKC) capacitance measurement amperometry

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Generation and Characterization of a Panel of Monoclonal Antibodies Against Distinct Epitopes of Human CD146

引用

生物物理学报 2009年第S1期25卷 231-232页

作者： Ping Wang1, Xiyun Yan.national laboratory of biomacromolecules, institute of biophysics, Chinese Academy of Sciences, 15 Datun Road,{2., china

CD146 (MUC18, Mel-CAM/MCAM) is a transmembrane protein, originally identified as a biomarker of melanoma, and plays an important role in cancer invasion and metastasis.

关键词： human CD146 antibody different epitopes

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Chronic morphine exposure induces degradation of receptive field properties of LGN cells in cats

引用

Acta Pharmacologica Sinica 2005年第9期26卷 1034-1038页

作者： Li-hua HE~2.Guang-xing LI~2.Xiang-mi LI~2.Yi-feng ZHOU~(2.3,4)2.Hefei national laboratory for Physical Sciences at Microscale and School of Life Science,University of Science and Technology of{2.,Hefei 2.002.,china 3 laboratory of Visual Information Processing,institute of biophysics,Chinese Academy of Sciences,beijing 100101,china Hefei national laboratory for Physical Sciences at Microscale and School of Life Science University of Science and Technology of China Hefei China laboratory of Visual Information Processing Institute of Biophysics Chinese Academy of Sciences Beijing China

Aim:To investigate the effect of chronic morphine exposure on the receptive fieldproperties of lateral geniculate nucleus(LGN)neurons in ***:Catswere injected with morphine(10 mg/kg)or saline twice daily,for 10 ***,extracellular single-unit recording techniques were used to examine the sensitivityof LGN neurons to visual stimuli in chronic morphine-treated and ***:Compared with saline-treated cats(as controls),LGN neurons inmorphine-treated cats had decreased signal-to-noise ratios(1.9 vs 3.1 ),and orien-tation and direction sensitivity(0.103 vs O.135 and 0.074 vs O.10,respectively),accompanied by significant increases in spontaneous(2..4 vs 17.5)and evokedactivity(preferred:42.2.vs 38.1;average:2..1 vs 2..3).Conclusion:Chronic mor-phine exposure can lead to the functional degradation of LGN neurons in cats,which might result from the effects of chronic morphine exposure on inhibitoryneurotransmission.

关键词： direction sensitivity orientation sensitivity signal-to-noise ratio spontaneous response evoked response

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Preliminary crystallographic studies on Des-(Phe-Val-Asn)^(B1-3)-insulin

引用

Chinese Science Bulletin 1997年第6期42卷 507-510页

作者： JIN Rongsheng, WAN Zhuli and LIANG DongcaiState Key laboratory of biomacromolecules,{2., Chinese Academy of Sciences, beijing 100101, Chin State Key laboratory of biomacromolecules Institute of Biophysics Chinese Academy of Sciences Beijing China

THE results of the X-ray analysis of the three-dimensional structure of insulin have shown that, in rhombohedral 2.Zn insulin, the N-terminal segment of the B-chain is on the surface of the monomer and dimmer of insulin molecules, assigning an extended conformation and high flexibility. With the difference of the surrounding solvent circumstances such as the crystallization condition of rhombohedral 4-Zn insulin, its configuration varies significantly: the

关键词： Des-(Phe-Val-Asn)B1-3 -insulin crystal growth X-ray diffraction.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Intrinsic peroxidase-like activity of ferromagnetic nanoparticles and its application in immunoassay and environment treatment

引用

生物物理学报 2009年第S1期25卷 304-305页

作者： Lizeng Gao, Jie Zhuang, Xiyun Yan national laboratory of biomacromolecules and institute of biophysics, Chinese Academy of Sciences, 15 Datun Road,{2., china.

Nanoparticles that contain magnetic materials, such as magnetite (Fe3O4), are particularly useful for imaging and separation techniques. Since these nanoparticles are

关键词： Nanoparticles Peroxidase immunoassay

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：