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screening agars for MRsA:evaluation of a stepwise diagnostic approach with two different selective agars for the screening for methicillin-resistant staphylococcus aureus(MRsA)

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Military Medical Research 2015年第4期3卷 213-219页

作者： Volker Micheel Benedikt hogan Thomas Koller Philipp Warnke sabine Crusius Rebecca Hinz Ralf Matthias Hagen Norbert Georg schwarz Hagen Frickmann Department of Tropical Medicine at the Bernhard Nocht Institute German Armed Forces Hospital of Hamburg Department of Infectious Disease Epidemiology Bernhard Nocht Institute for Tropical Medicine Hamburg Institute for Microbiology Virology and Hygiene University Medicine Rostock

Background: Colonization with methicillin-resistant staphylococcus aureus(MRsA) poses a hygiene risk that does not spare field hospitals or military medical field camps during military deployments. Diagnostic options for unambiguously identifying MRsA isolates are usually scarce in military environments. In this study, we assessed the stepwise application of two different selective agars for the specific identification of MRsA in screening ***: Nasal swabs from 1,541 volunteers were subjected to thioglycollate broth enrichment and subsequently screened on CHROMagar MRsA selective agar for the identification of MRsA. The MRsA identity of suspiciouslooking colonies was confirmed afterwards or excluded by another selective agar, chrom ID MRsA. All isolates from the selective agars with MRsA-specific colony morphology were identified by biochemical methods and mass ***: The initial CHROMagar MRsA screening identified suspicious colonies in 36 out of 1541 samples. A total of 25 of these 36 isolates showed MRsA-like growth on chrom ID agar. Out of these 25 isolates, 24 were confirmed as MRsA, while one isolate was identified as staphylococcus kloosii. From the 11 strains that did not show suspicious growth on chrom ID agar, 3 were methicillin-sensitive staphylococcus aureus(MssA, with one instance of cocolonization with Corynebacterium spp.), 2 were confirmed as MRsA(with 1 instance of co-colonization with MssA), 2 were lost during passaging and could not be re-cultured, one could not be identified by the applied approaches, and the remaining 3 strains were identified as staphylococcus saprophyticus, staphylococcus hominis(co-colonized with Macrococcus caseolyticus) and staphylococcus cohnii, ***: The application of the selective agar CHROMagar MRsA alone proved to be too non-specific to allow for a reliable diagnosis of the presence of MRsA. The combined use of two selective agars in a stepwise approach reduced this non-s

关键词： Methicillin-resistant staphylococcus aureus selective agar Chromogenic agar screening Hygiene stepwise diagnostics CHROMagar Chrom ID agar

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Traditional Healer’s Medicinal Practice survey and Clinical Evidence Assessment of “YIKI”: An Antimalarial Phytomedicine Recipe from Bobo-Dioulasso, Burkina Faso

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Pharmacology & Pharmacy（药理与制药） 2024年第7期 248页

作者： Zachari Kabre Rakiswende serge Yerbanga Aminata Fofana Roland Nâg-Tiero Meda Anyirekun Fabrice some Alassane Haro natacha A. J. A. Boro Fazhira B. Ye Benjamin Kouliga Koama sami Eric Kam Eliasse Zongo Hadidjatou Belèm Windmi Kagambèga Florence Fournet Georges A. Ouedraogo Jean-Bosco Ouedraogo

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Impact of Different Light Characteristics on the Growth and Lipid Content of Diatom Phaeodactylum tricornutum Transconjugant strains

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American Journal of Plant sciences 2023年第1期14卷 41-63页

作者： Nikunj sharma Elisa Ines Fantino Fatima Awwad natacha Mérindol Arun Augustine Fatma Meddeb Isabel Desgagné-Penix Department of Chemistry Biochemistry and Physics Université du Québec à Trois-Rivières Trois-Rivières Québec Canada

Light regulates important metabolic processes in microalgal cells, which can further impact the metabolism and the accumulation of biomolecules such as lipids, carbohydrates, and proteins. Different characteristics of light have been studied on various strains of the model diatom Phaeodactylum tricornutum, but not on transconjugant cells and information on wild-type strains is still limited. Therefore, we studied the impact of different light characteristics such as spectral quality, light intensity and light shift on the growth, and the composition in lipids and fatty acids of P. tricornutum cells to provide a comprehensive context for future applications. Initially, we tested the impact of spectral quality and light intensity on P. tricornutum transformed with an episomal vector (Ptev), harboring the resistance gene sh ble. Results indicated that Ptev cells accumulated more biomass and overall lipids in spectral quality Red 1 (R1: 34% > 600 nm > 66%) more effectively as compared to Red 2 (R2: 8% > 600 nm > 92%). It was also detected that cell granularity was higher in R1 as compared to R2. Furthermore, by testing two light intensities 65 μmol·msup>-2sup>·ssup>-1sup> and 145 μmol·msup>-2sup>·ssup>-1sup> light, it was observed that 145 μmol·msup>-2sup>·ssup>-1sup> led to an increase in growth trend, total biomass and lipid content. Combining spectral qualities and light intensities, we show that the lipid accumulation raised by 2.8-fold. studying the light intensity and spectral quality allowed us to optimize the light conditions to R1 spectral quality and light intensity 145 μmol·msup>-2sup>·ssup>-1sup>. These initial results showed that red light R1 at 145 μmol·msup>-2sup>·ssup>-1sup> was the best condition for biomass and total lipids accumulation in Ptev cells. Next, we further combined these two-light optimizations with a third light characteristics, i.e. light s

关键词： Phaeodactylum tricornutum Light Condition Episomal Vector Dia-toms Fatty Acids Biomass

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Management of Malaria in Pregnant Women at the Maternity Unit of the University Teaching Hospital Yalgado Ouedraogo (UTH-YO) in Ouagadougou: About Report on the Quality of Care

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Open Journal of Obstetrics and Gynecology 2018年第13期8卷 1337-1344页

作者： Ouattara Adama Ouédraogo smaila Lankoandé Bako Coulibaly natacha Ouédraogo Marie Charlemagne Ouédraogo Ali Thieba Bonané Blandine Training and Research Unit in Health sciences (TRU/Hs) University Ouaga 1 Pr Joseph KI ZERBO Ouagadougou Burkina Faso Department of Gynecology and Obstetrics The University Teaching Hospital Yalgado Ouedraogo of Ouagadougou (UTH-YO) Ouagadougou Burkina Faso Department of Gynecology and Obstetrics The Bogodogo University Teaching Hospital of Ouagadougou (UTH-B) Ouagadougou Burkina Faso

Objective: To do a report on the quality of the treatment of malaria in pregnant women at University Teaching Hospital Yalgado Ouedraogo (UTH-YO) in Ouagadougou. Materials and Methods: This was a descriptive cross-sectional study conducted from 29 July to 02 October 2016. The targets were pregnant women and gynecology and obstetrics service care providers. The study used an individual questionnaire administered and clinical parasitological research by the Rapid Diagnostic Test (RDT). some information was verified in the patient’s prenatal consultation logbook. A total of 351 women and 44 providers participated in the study. The data was captured on a microcomputer and analyzed using Epi info version 7 software. Results: Regarding preventive measures, patients’ responses were correct for long-lasting impregnated mosquito net (LLIN) in 98.8% of cases and for intermittent preventive treatment (IPT) in 96.5% of cases. About providers, they had given a good answer in all cases for using LLIN and taking IPT. Regarding care providers’ knowledge of the clinical signs of malaria, fever was cited in 95.5% of cases for simple malaria and Frankish jaundice in 97.7% of cases for severe malaria. With regard to curative treatment, 77.3% of the care providers surveyed stated that quinine was the reference molecule for the treatment of simple malaria in pregnant women. In terms of the availability of inputs used for malaria management, our survey found no RDTs. Also, the only molecule available was quinine in its compressed and injectable forms. Conclusion: Care Providers’ and patients’ knowledge of malaria in the obstetrics and genecology department is quite satisfactory. However, some shortcomings were noted in the provision of screening and curative and preventive treatment. The elimination of malaria in our country will require joint efforts at all levels.

关键词： Malaria Pregnancy Treatment UTH-YO

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Nano-additive manufacturing of multilevel strengthened aluminum matrix composites

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International Journal of Extreme Manufacturing 2023年第1期5卷 254-264页

作者： Chenwei shao Haoyang Li Yankun Zhu Peng Li Haoyang Yu Zhefeng Zhang Herbert Gleiter AndréMcDonald James.hogan Department of Mechanical Engineering University of AlbertaEdmonton T6G 1H9Canada shi-Changxu Innovation Center for Advanced Materials Institute of Metal ResearchChinese Academy of SciencesShenyang 110016People’s Republic of China school of Materials science and Engineering University of Science and Technology of ChinaHefei 230026People’s Republic of China nanoFAB Fabrication and Characterization Centre University of AlbertaEdmonton T6G 1H9Canada Institute of Nanotechnology Karlsruhe Institute of TechnologyHermann-von-Helmholtz-Platz 1Eggenstein-Leopoldshafen 76344Germany

Nanostructured materials are being actively developed,while it remains an open question how to rapidly scale them up to bulk engineering materials for broad industrial *** study propose an industrial approach to rapidly fabricate high-strength large-size nanostructured metal matrix composites and attempts to investigate and optimize the deposition process and strengthening ***,advanced nanocrystalline aluminum matrix composites(nanoAMCs)were assembled for the first time by a novel nano-additive manufacturing method that was guided by numerical simulations(*** in-flight particle model and the porefree deposition model).The present nanoAMC with a mean grain sizess eight times higher than the bulk aluminum and shows the highest hardness among all Al–Al2O3 composites reported to date in the literature,which are the outcome of controlling multiscale strengthening mechanisms from tailoring solution atoms,dislocations,grain boundaries,precipitates,and externally introduced reinforcing *** present high-throughput strategy and method can be extended to design and architect advanced coatings or bulk materials in a highly efficient(synthesizing a nanostructured bulk with dimensions of 50×20×4 mm^(3) in 9 min)and highly flexible(regulating the gradient microstructures in bulk)way,which is conducive to industrial production and application.

关键词： high-throughput fabrication bulk nanoAMC low-temperature additive manufacturing multi-level strengthening

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中国在全球治理中的支柱作用:气候变化与人权(英文)

中国在全球治理中的支柱作用:气候变化与人权(英文)

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北京论坛（2010）文明的和谐与共同繁荣——为了我们共同的家园：责任与行动

作者： Jaechun Kim natacha Moorooven sogang University

*** James Kynge,author of the book entitled China shakes The World.A Titan’s Rise and Troubled Future-and the Challenge for America,declares on his award winning night"In a world that is interconnected to an unprecedented degree,we are all living pieces of each other’s *** share not only an increasingly common future and present *** as much as history. configures our current realities,we are also sharing a common *** biggest new influenceby almost any measure-on this globalised world has been China’s *** are finding that the trends shaping our businesses,politics,environment and strategic outlook are increasingly‘Made in China’."Another China specialist Gill claims."China plays a critical role on global issues,from nonproliferation to climate change,and is at the heart of contentious national debates on such issues as job losses,trade deficits,and human rights".

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16岁以上肝硬化患者的评估和管理：NICE指南概要

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英国医学杂志中文版 2017年第7期20卷 402-404页

作者： P Harrison B J hogan L Floros health London,UK 马莉杨红 Kings College Hospital London SE5 9RS UK Guys's & st Thomas' Hospitals NHs Foundation Trust London UK National Guideline Centre Royal College of Physicians London NW14LE UK 北京协和医院消化科

肝硬化的临床识别困难，特别是代偿期肝硬化患者大多无症状，诊断难度更大。关于哪些患者需要进行肝硬化检查，选择何种检查方法，英格兰和威尔士地区的临床实践缺乏统一。因此我们推出本指南，旨在为临床提供帮助，包括肝硬化的诊断、... 详细信息

肝硬化的临床识别困难，特别是代偿期肝硬化患者大多无症状，诊断难度更大。关于哪些患者需要进行肝硬化检查，选择何种检查方法，英格兰和威尔士地区的临床实践缺乏统一。因此我们推出本指南，旨在为临床提供帮助，包括肝硬化的诊断、将有失代偿风险的患者及时转诊给专科医师，以及肝硬化患者的管理——包括监测和治疗并发症等方面。

关键词：肝硬化患者评估方法临床识别临床分析

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气溶胶线性吸收系数及其吸收可见辐射的研究

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南京大学学报(自然科学) 1988年第1期 130-135页

作者：黄世鸿 A.W.hogan 南京大学大气科学系纽约州立大学奥尔巴尼分校大气科学研究中心

基于测量气溶胶吸收系数的粒子采样法,导出了表征气溶胶总体吸收性能的线性吸收系数E,从而简化了测量设备和手续,为研究气溶胶辐射吸收性能提供廉价而简便的测量工具。分析和实验表明,E实质上是Beer—Lambart定律中吸收系数Ka的合理近... 详细信息

基于测量气溶胶吸收系数的粒子采样法,导出了表征气溶胶总体吸收性能的线性吸收系数E,从而简化了测量设备和手续,为研究气溶胶辐射吸收性能提供廉价而简便的测量工具。分析和实验表明,E实质上是Beer—Lambart定律中吸收系数Ka的合理近似。在纽约州奥尔巴尼和南京近郊外场观测表明,E值分别是10-10M和10M。工业城市和排放源的下风方的E值普遍较大。E值与能见度呈反向趋势。

关键词：粒子采样法总体吸收性能线性吸收系数

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Handbook of stem Cells

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丛书名： v. 1

2004年

作者： Robert Lanza John Gearhart Brigid hogan Douglas Melton Roger Pedersen James Thomson Michael West

ISBN: (数字)9780080533735

ISBN: (纸本)9780124366435

New discoveries in the field of stem cell research have frequently appeared in the news and in scientific literature. Research in this area promises to lead to new therapies for cancer, heart disease, diabetes, and a wide variety of other diseases. This two-volume reference integrates this exciting area of biology, combining the prerequisites for a general understanding of adult and embryonic stem cells, the tools, methods, and experimental protocols needed to study and characterize stem cells and progenitor populations, as well as a presentation by the world's experts of what is currently known about each specific organ system. The editors of the Handbook of stem Cells include: Robert Lanza, Helen Blau, John Gearhart, Brigid hogan, Douglas Melton, Malcolm Moore, Roger Pedersen, E. Donnall Thomas, James Thomson, Catherine Verfaillie, Irving Weissman, and Michael West. The Editorial Board includes: W. French Anderson, Peter Andrews, Anthony Atala, Jose Cibelli, Giulio Cossu, Robert Edwards, Martin Evans, Elaine Fuchs, Margaret Fuller, Fred Gage, Richard Gardner, Margaret Goodell, Ronald Green, William Haseltine, Joseph Itskovitz-Eldor, Rudolf Jaenisch, Ihor Lemischka, Dame Anne McLaren, Richard Mulligan, stuart Orkin, Martin Pera, Benjamin Reubinoff, Janet Rossant, Hans scholer, Austin smith, Evan snyder, Davor solter, Alan Trounson, and Leonard Zon. This comprehensive set should be a much-needed addition to the library of students and researchers alike.

关键词： stem cells.

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Methicillin-Resistant <i>staphylococcus aureus</i>sCC<i>mec</i>Type and Its Association with Clinical Presentation, severity, and Length of stay among Patients with Complicated skin and skin structure Infections

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Advances in Infectious Diseases 2014年第2期4卷 111-115页

作者： Eric Gomez Tom Chiang Patricia A. hogan Daniela E. Myers David B. Huang Appalachian Regional Healthcare Beckley USA Pfizer Inc. Collegeville USA Rutgers Medical school Newark USA Veterans Affairs New Jersey Healthcare system (VA NJHCs) East Orange USA

Data from a prospective, randomized, open-label, active-controlled, multicenter, Phase 4 study comparing oral or intravenous linezolid with intravenous vancomycin for treatment of complicated skin and soft-tissue infections caused by methicillin resistant staphylococcus aureus was used to determine the association between staphylococcal cassette chromosome mec (sCCmec) type and patient’s clinical presentation, infection severity, intravenous therapy duration and length of stay (LOs). Compared to sCCmec types I, II, and III, sCCmec type IV, PVL+ was associated with more frequent presentation of abscesses, lower severity scores, and shorter intravenous therapy duration and LOs in both treatment groups.

关键词： MRsA sCCmec Type Infection severity Length of stay

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