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protective effects of Amaranthus hybridus against aflatoxin B1 and fumonisin B1-induced genotoxicity in H4IIE-luc cells

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Hepatoma research 2015年第1期1卷 136-146页

作者： Mohamed I.M.Ibrahim rialet pieters Sekena H.Abdel-Aziem Anna M.vander Walt Cornelius C.Bezuidenhout John p.Giesy Mosaad A.Abdel-Wahhab Food Toxicology and Contaminants Department National Research CenterDokkiCairo 12622Egypt Unit of Environmental Sciences and Management North-West UniversityPrivate Bag X6001Potchefstroom 2520South Africa Cell Biology Department National Research CenterDokkiCairo 12622Egypt Department of Veterinary Biomedical Sciences and Toxicology Centre University of SaskatchewanSaskatoonSaskatchewan S7N 5B4Canada Department of Zoology and Center for Integrative ToxicologyMichigan State UniversityEast LansingMI 48824USA Department of Biology and Chemistry and state key laboratory in Marine {3. City University of Hong KongKowloonHong KongChina School of Biological Sciences University of Hong KongHong KongChina state key laboratory of {3. control and resource reuse School of the EnvironmentNanjing UniversityNanjing 210093JiangsuChina Department of Biology Hong Kong Baptist UniversityHong KongChina

Aim:protective effects of aqueous extract of Amaranthus hybridus against afl atoxin B1(AFB_(1))and/or fumonisin B1(FB_(1))on the H4IIE-luc cell line were determined by use of the methyl thiazol tetrazolium viability assay and disruption of DNA ***:H4IIE-luc cells were incubated with different concentrations of AFB_(1) and/or FB_(1) for 24 and 48 h with or without aqueous extract of ***:AFB_(1) decreased the viability of cells after 24 and 48 h of ***_(50)values for AFB_(1) were 10.5 and 1.8μmol/L for the two periods,*** the 48 h exposure to mycotoxin repeated with a pre-treatment of 20 and 40μg/mL extract of ***,the EC_(50)changed to 3.88 and 7.67μmol/L,respectively.H4IIE-luc cells exposed to FB_(1) for 24 h responded more than those incubated for 48 *** treated with a combination of AFB_(1) and FB_(1) were less viable with a signifi cant decrease in the greater *** mixture of AFB_(1) and FB_(1) resulted in a signifi cant threat to H4IIE-luc as indicated by the absence or appearance of new bands in random amplifi ed polymorphic DNA analysis,which demonstrated damage to *** protective effects were probably due to greater content of total phenolics,carotenoids,β-carotene,folic-,linolenic-,linoleic and palmitic acids,as well as calcium,magnesium,iron,zinc,and selenium observed in the ***:Exposure to 40μg/mL of extract of *** protected cells from damage to DNA by stabilizing DNA.

关键词： Afl atoxin B1 Amaranthus hybridus cytotoxicity DNA fumonisin B1 hepatoma cells methyl thiazol tetrazolium assay

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Aqueous extract of Corchorus olitorius decreases cytotoxicityof aflatoxin B1 and fumonisin B1 in H4IIE-luc cells

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Hepatoma research 2015年第1期1卷 75-85页

作者： Mohamed I.M.Ibrahim rialet pieters Sekena H.Abdel-Aziem Anna M.van der Walt Cornelius C.Bezuidenhout John p.Giesy Mosaad A.Abdel-Wahhab Department of Food Toxicology and Contaminants National Research CentreDokkiCairo 12622Egypt Unit of Environmental Sciences and Management North-West UniversityPrivate Bag X6001Potchefstroom 2520South Africa Department of Cell Biology National Research CentreDokkiCairo 12622Egypt Department of Veterinary Biomedical Sciences and Toxicology Centre University of SaskatchewanSaskatoonSaskatchewan S7N 5B4Canada Department of Zoology and Center for Integrative ToxicologyMichigan State UniversityEast LansingMI 48824USA Department of Biology and Chemistry and state key laboratory in Marine {3. City University of Hong KongKowloonHong KongChina School of Biological Sciences University of Hong KongHong KongChina state key laboratory of {3. control and resource reuse School of the EnvironmentNanjing UniversityNanjing 210093JiangsuChina Department of Biology Hong Kong Baptist UniversityHong KongChina

Aim:Afl atoxin B1(AFB1)and fumonisin B1(FB1)are important food-borne ***-contamination of foodstuffs with these two mycotoxins is well-known and has been implicated in a possible development of hepatocellular carcinoma in humans living in regions of the world where exposures to these mycotoxins in grain are *** aim of the current study was to evaluate the potential protective effects of an aqueous extract of Cochorus olitorius(***,moroheiya)against cytotoxicity of AFB1 and/or FB1 in H4IIE-luc rat hepatoma cells,using assays to measure cell viability and disruption of DNA *** this transactivation assay was originally developed to specifi cally respond to aryl hydrocarbon agonists,this cell line was used because of its hepatic ***:H4IIE-luc cells were incubated with different concentrations of AFB1 and/or FB1 for 24 and 48 h with or without aqueous extract of ***:Both mycotoxins decreased cell viability and increased DNA *** was more pronounced when cells were exposed simultaneously to AFB1 and ***:Aqueous extract of *** protected cells against cytotoxicity of *** contains a water-soluble,natural chemo-preventative agent for cancer that should be isolated and identifi ed.

关键词： Anticancer cytotoxicity DNA liver moroheiya mycotoxins

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