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Seed Priming and Foliar Supplementation withβ-aminobutyric Acid Alleviates Drought Stress through Mitigation of Oxidative Stress and Enhancement of Antioxidant Defense in Linseed(Linum usitatissimum L.)

引用

Phyton-International Journal of Experimental Botany 2023年第11期92卷 3113-3131页

作者： Tauqeer Ahmad Yasir Muhammad Ateeq Allah Wasaya Mubshar Hussain naeem sarwar Khuram Mubeen Mudassir Aziz Muhammad Aamir Iqbal Chukwuma COgbaga Ibrahim Al-Ashkar Md Atikur Rahman Ayman El Sabagh Department of Agronomy University of LayyahLayyah31200Pakistan Department of Agronomy Bahauddin Zakariya UniversityMultan60000Pakistan State Key Lab of Crop Stress Biology for Arid Areas College of AgronomyNorthwest A&F UniversityYangling712100China Department of Agronomy MNS University of AgricultureMultan60000Pakistan Department of Agronomy Faculty of AgricultureUniversity of Poonch RawalakotRawalakot10250Pakistan Independent Researcher MiddlesbroughNorth YorkshireUK Plant Production Department College of Food and Agriculture SciencesKing Saud UniversityP.O.Box 2460Riyadh11451Saudi Arabia Grassland and Forage Division National Institute of Animal ScienceRural Development AdministrationCheonan31000Korea Department of Field Crops Faculty of AgricultureSiirt UniversitySiirt56100Turkey Department of Agronomy Faculty of AgricultureKafrelsheikh UniversityKafr El-Shaikh33516Egypt

Drought is one of the critical limitations to agricultural soils and crop *** of water is increasing due to climate change that lead to increasing threats to global food ***,ecofriendly and cost effective strategies are highly desirable for mitigating drought stress along with sustainable and smart agricultural *** aim of the study was to mitigate DS using seed priming and exogenous supplementation ofβ-aminobutyric acid(BABA)in linseed(Linum usitatissimum L.).Different doses(0,50,100 and 150μM)of BABA were used for seed priming agent and foliar spraying under three soil moisture levels viz.,25%(SM25),45%(SM45)and 65%(SM65).The response variables of both experiments included different agro-botanical traits and oxidative stress indicators such as melondialdehyde content,free proline accumulation,and antioxidant defense in *** linseed plants showed water stress at SM25 that reduced plant height,number of branches per plant,time taken to flower initiation and heading,and root and shoot dry ***,the number of capsules and seeds per capsule showed a significant decline at SM25,which led to a drastic reduction in 100-seed weight yield in linseed plants in both ***,seed priming and foliar supplementation with of BABA(50–100μM)significantly improved these morpho-agronomical attributes in linseed plants under *** results revealed that the BABA was fully active in linseed plants at ***,the combination of SM25 with BABA significantly improved the antioxidant enzymes superoxide dismutase(SOD),catalase(CAT),ascorbate peroxidase(APX),and peroxidase(POD)activity,which significantly enhanced DS tolerance in linseed *** findings might be useful to oil seed breeders and farmers linseed for breeding program in linseed plants as well as sustainable agricultural production of oil seed crop plants.

关键词： Linseed water stress signaling molecule climate change biostimulant ecofriendly approach sustainable agricultural

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Assessment of biosafety implementation in clinical diagnostic laboratories in Pakistan during the COVID-19 pandemic

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Journal of Biosafety and Biosecurity 2022年第1期4卷 43-49页

作者： Samreen sarwar Faheem Shahzad Ayesha Vajeeha Rimsha Munir Amina Yaqoob Aniqa naeem Mamoona Sattar Sheereen Gull Health Security Partners LahorePunjabPakistan Department of Immunology University of Health Sciences LahorePunjabPakistan Centre for Applied Molecular Biology University of the PunjabLahorePunjabPakistan Cancer Biology Lab Institute of Microbiology and Molecular GeneticsUniversity of the Punjaband Molecular GeneticistHormone LabLahorePunjabPakistan Center of Excellence in Molecular Biology University of the PunjabLahorePakistan Food and Meal Science Kristianstad UniversityKristianstadSweden Microbiological Engineering and Industrial Biotechnology Group College of ChemistryChemical Engineering and BiotechnologyDonghua UniversityShanghaiChina School of Biological Sciences University of the PunjabLahorePakistan

Laboratory diagnostic capacity is crucial for an optimal national response to a public health emergency such as the COVID-19 *** laboratory-acquired infections and the loss of critical human resources,especially during a public health emergency,requires laboratories to have a good biorisk management system in *** this study,we aimed to evaluate laboratory biosafety and biosecurity in Pakistan during the COVID-19 *** this cross-sectional study,a self-rated anonymous questionnaire was distributed to laboratory professionals(LPs)working in clinical diagnostic laboratories,including laboratories performing polymerase chain reaction(PCR)-based COVID-19 diagnostic testing in Punjab,Sindh,Khyber Pakhtunkhwa,and Gilgit-Baltistan provinces as well as Islamabad during March 2020 to April *** questionnaire assessed knowledge and perceptions of LPs,resource availability,and commitment by top management in these *** total,58.6%of LPs performing COVID-19 testing reported that their laboratory did not conduct a biorisk assessment before starting COVID-19 testing in their *** 31%of LPs were aware that COVID-19 testing could be performed at a biosafety level 2 laboratory,as per the World Health Organization interim biosafety guidelines.A sufficiently high percentage of LPs did not feel confident in their ability to handle COVID-19 samples(32.8%),spills(43.1%),or other accidents(32.8%).These findings demonstrate the need for effective biosafety program implementation,proper training,and establishing competency assessment *** findings also suggested that identifying and addressing gaps in existing biorisk management systems through sustainable interventions and preparing LPs for surge capacity is crucial to better address public health emergencies.

关键词： Biosafety Laboratory professionals COVID-19 pandemic

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