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Acta Oceanologica Sinica 2023年第4期42卷 72-80页

作者： Chenyu song Zhong Tu na song Key Laboratory of Mariculture(Ocean University of China) Ministry of EducationQingdao 266003China Shandong Fisheries Development and Resources Conservation Center Yantai 264003China

Acanthogobius ommaturus, which belongs to the family Gobiidae, is a euryhaline and demersal fish that is widely distributed in the coastal areas, harbors, and estuaries of China, D. P. R. Korea and Japan. In this study, the genetic diversity and genetic structure of five geographical populations of A. ommaturus was assessed using the mitochondrial hypervariable region gene and microsatellite markers. The results of the two genetic markers indicated that the A. ommaturus populations had a high level of genetic diversity. The mitochondrial marker detected weak genetic differentiation among populations, and the Neighbor-Joining tree showed that there was no obvious pedigree branches and geographic structure as well. However, population of Zhoushan showed significant genetic differentiation with other populations by microsatellite markers. The population of *** has not experienced bottleneck effect recently. We speculated that the Pleistocene climate change and juvenile fish dispersal played an important role in the population differentiation of A. ommaturus.

关键词： mitochondrial markers microsatellite markers genetic diversity genetic structure Acanthogobius ommaturus

A patatin-like protein synergistically regulated by jasmonate and ethylene signaling pathways plays a negative role in Nicotiana attenuata resistance to Alternaria alternata

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Plant Diversity 2019年第1期41卷 7-12页

作者： Junbin Cheng na song Jinsong Wu Yunnan Key Laboratory for Wild Plant Resources Kunming Institute of Botany Chinese Academy of Sciences University of Chinese Academy of Sciences

Although patatin was initially identified as a major storage protein in potato tubers, patatin-like proteins(PLPs) have been recently reported to be widely present in many plant species and shown to be involved in plant-pathogen interactions. However, it is not clear whether PLPs are involved in Nicotiana attenuata resistance against the necrotrophic fungal pathogen, Alternaria alternata. In this study we identified a naPLP gene, whose expression was highly elicited by A. alternata inoculation. Silencing naPLP enhanced N. attenuata resistance to A. alternata, which was associated with higher induction levels of JA and ethylene biosynthetic genes, naACS1, naACO1 and naLOX3. The induction of naPLP expression by the fungus was abolished in JA-deficient plants and significantly reduced in ethylene-insensitive plants. In addition, naPLP transcripts were highly induced by exogenous treatment with either methyl jasmonate(MeJA) or ethephon. Co-treatment with MeJA and ethephon led to a much higher induction level of naPLP transcripts, and this synergistic induction was largely dependent on endogenous JA and ethylene signaling pathways. Thus, we conclude that the naPLP gene is elicited by A. alternata via JA and ethylene signaling pathways in a synergistic way; however, unlike other JA-and ethylene-induced defense genes,naPLP negatively affects plant resistance to the fungus likely by suppressing JA and ethylene biosynthetic gene expression.

关键词： Alternaria Ethylene Jasmonate Necrotrophic fungal pathogen Nicotiana Plant resistance

Profile of candidate microsatellite markers in Sebastiscus marmoratus using 454 pyrosequencing

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Chinese Journal of Oceanology and Limnology 2017年第1期35卷 198-202页

作者： song na Fisheries College Ocean University of China Qingdao 266000 China Fisheries College Zhejiang Ocean University Zhoushan 316000 China national Research Institute of Fisheries Science Fisheries Research Agency Yokohama Japan

Sebastiscus marmoratus is an important sedentary ovoviparous fish distributed in near-shore coastal waters from the coast of China to Japan. Candidate S. marmoratus microsatellite markers were developed in the present study using 454 pyrosequencing, and the marker profile was analyzed. A total of 2 000 000 raw sequence reads were assembled to reduce redundancy. Among them, 1 043 dinucleotide, 925 trinucleotide, 692 tetranucleotide, and 315 pentanucleotide repeats were detected. AC repeats were the most frequent motifs among the dinucleotide repeats, and AAT was the most abundant among the trinucleotide repeats. AAAT, ATAG, and ATCC were the three most common tetranucleotide motifs, and AAGAT and AATAT were the most dominant pentanucleotide motifs. The greatest numbers of loci and potentially amplifiable loci were found in dinucleotide repeats, whereas trinucleotide repeats had the fewest. In summary, a wide range of candidate microsatellite markers were identified in the present study using a rapid and efficient 454 pyrosequencing approach.

关键词： marbled rockfish microsatellite 454 FLX pyrosequencing genomic Dna

The mitochondrial genome of Chaeturichthys stigmatias provides novel insight into the interspecific difference with Amblychaeturichthys hexanema

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Acta Oceanologica Sinica 2021年第9期40卷 74-81页

作者： Jian Zheng Bingjie Chen Tianxiang Gao na song Key Laboratory of Mariculture of Ministry of Education Ocean University of ChinaQingdao 266003China Fishery College Zhejiang Ocean UniversityZhoushan 316022China

Chaeturichthys stigmatias and Amblychaeturichthys hexanema belong to the family Gobiidae,which are offshore warm fish species and widely distribute in the western Pacific *** this study,the mitochondrial cytochrome c oxidase subunit I(COI)sequences and 12 S ribosomal Rna(12 S rRna)sequences were used to analyze the interspecific differences between the two *** phylogenetic analysis showed that the interspecific distance was significantly higher than the intraspecific genetic *** Neighbor-Joining tree showed two separate clusters,without sharing *** mitochondrial genome sequence of *** was also *** genome was 17134 bp in size,with a high A+T content of 55.9%.The phylogenetic analysis based on the tandem 13 coding protein genes nucleotide sequences indicated that *** showed a close relationship with *** study can provide the basic genetic data for two species and will help for constructing the phylogeny of the Gobiiade.

关键词： Chaeturichthys stigmatias Amblychaeturichthys hexanema mitochondrial genome phylogenetic analysis

Genetic variation of the small yellow croaker(Larimichthys polyactis)inferred from mitochondrial Dna provides novel insight into the fluctuation of resources

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Acta Oceanologica Sinica 2022年第11期41卷 88-95页

作者： Jian Zheng Tianxiang Gao Yunrong Yan na song Key Laboratory of Mariculture of Ministry of Education Ocean University of ChinaQingdao 266003China Fishery College Zhejiang Ocean UniversityZhoushan 316022China College of Fisheries Guangdong Ocean UniversityZhanjiang 524088China

The small yellow croaker(Larimichthys polyactis)belongs to the family Sciaenidae,which is an offshore warm fish species and widely distributed in the western *** this study,the variation of genetic diversity and genetic differentiation among *** populations was analyzed by mitochondrial Dna control region.A total of 110 polymorphic sites were checked,which defined 134 *** level of haplotype diversity(h=0.993±0.002)was detected in the examined *** genetic structure analyse(analysis of molecular variance,Fst)showed there were high gene flow among *** *** result showed that there were relatively high genetic diversity and low genetic differentiation among the Yellow Sea and the East China Sea populations,which can be attributed to diverse habitats,wide distribution range and high mutation rate of control *** phylogenetic methods,coalescent analyses(neutrality tests,mismatch distribution analysis,Bayesian skyline analyses)and molecular dating interpreted in conjunction with paleoclimatic and physiographic evidence,we inferred that the genetic make-up of extant populations of *** was shaped by Pleistocene environmental impacts on the historical demography of this ***,relatively constant genetic diversity and larger effective population size were detected in recent *** *** result showed that the fishing policy certainly,such as the summer closed fishing,played a role in protecting resources of *** study can offer a wealth of biological novelties which indicates genetic structure of *** population and provides the foundation for resources protection and policy setting.

关键词： Larimichthys polyactis genetic diversity genetic differentiation mitochondrial Dna control region

Interconnected MXene/Graphene Network Constructed by Soft Template for Multi‑Performance Improvement of Polymer Composites

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nano-Micro Letters 2022年第8期14卷 164-180页

作者： Liyuan Jin Wenjing Cao Pei Wang na song Peng Ding Research Center of nanoscience and nanotechnology College of SciencesShanghai UniversityShanghai 200444

The multi-functionalization of polymer composites refers to the ability to connect multiple properties through simple structural design and simultaneously achieve multi-performance *** large-scale design and mass production to realize the reasonable structure design of multifunctional polymer composites are urgently remaining ***,the multifunctional MXene/graphene/polymer composites with three-dimensional thermally and electrically conductive network structures are fabricated via the utilization of the microstructure of the soft template,and a facile dispersion dip-coating *** a result,the polymer composites have a multiperformance *** the MXene and graphene content of 18.7 wt%,the superior throughplane thermal conductivity of polymer composite is 2.44 W m^(−1)K^(−1),which is 1118%higher than that of the polymer *** electromagnetic interference(EMI)shielding effectiveness of the sample reaches 43.3 dB in the range of *** the mechanical property of the sample has advanced 4 times compared with the polymer *** excellent EMI shielding and thermal management performance,along with the effortless and easy-to-scalable producing techniques,imply promising perspectives of the polymer composites in the next-generation smart electronic devices.

关键词： Structural design MXene/graphene network Soft-template Thermal conductivity Electromagnetic interference shielding

Research and design of wap service system based on MISC platform

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The Journal of China Universities of Posts and Telecommunications 2006年第4期13卷 34-38页

作者： YU Oun song Mei-na song Jun-de ZHAN Xiao-su PCN and CAD Center Beijing University of Posts and Telecommunications Beijing 100876 China

Wireless application protocol （WAP） have become the available method for subscribers to access mobile Internet through mobile terminal, regardless of the location and time. In this article, a logical architecture of WAP service system based on mobile information service centre （MISC） [1, 2] platform is discussed. The whole system is designed and developed on Java 2 enterprise edition [3] （J2EE） architecture and deployed on BEA WebLogic Server [3, 4]. Furthermore, a series of various WAP services based on this system have been implemented, which validates the practice value of this system. This optimal scheme presented in this article will reduce the development complexity, deployment risk, and so on.

关键词： WAP MISC J2EE WAP Engine MBlog

Two new prenylflavonoids from Epimedium sutchuenense

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Chinese Chemical Letters 2009年第7期20卷 842-844页

作者： Chang Yuan Yu Li na song Guang Chen College of Life Science and Technology Beijing University of Chemical Technology Beijing 100029 China

Two new prenylflavonoids, named sutchuenmedin A （1） and sutchuenmedin B （2）, were isolated from the 70% EtOH extract of Epimedium sutchuenense. Their structures were determined from spectral analysis. C 2009 Guang... 详细信息

关键词： Epimedium sutchuenense Flavonoids Sutchuenmedin A Sutchuenmedin B

Profile and development of microsatellite primers for Acanthogobius ommaturus based on high-throughput sequencing technology

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Journal of Oceanology and Limnology 2020年第6期38卷 1880-1890页

作者： song Chenyu FENG Ziyi LI Chunhou SUN Zhicheng GAO Tianxiang song na LIU Lu Fisheries College Ocean University of ChinaQingdao 266003China South China Sea Fisheries Research Institute Chinese Academy of Fishery SciencesGuangzhou 510300China national Engineering Research Center for Marine Aquaculture Zhejiang Ocean UniversityZhoushan 316022China

Acanthogobius ommaturus,a fish species of the Family Gobiidae,is a marine commercial fish perched on the bottom of *** this study,Illumina high-throughput sequencing technology was applied to obtain the candidate microsatellite markers of ***.A total of 4746 microsatellite-rich fragments were found,of which 4542 microsatellites are with primer fragments,containing 971 dinucleotide sequences,2643 trinucleotide sequences,569 tetranucleotide sequences,406 pentanucleotide sequences,and 212 hexanucleotide *** on the results of high-throughput sequencing,a total of 141 pairs of the microsatellite primers were designed and *** then 24 polymorphic primers were finally obtained by polyacrylamide gel *** total,271 alleles were detected in the 24 pairs of *** number of alleles for different primers ranged from 5 to *** average number of effective alleles(na)was 11.292;the average observed heterozygosity(Ho)of the 24 pairs of primers was 0.665,the average expected heterozygosity(He)was 0.880,and the average polymorphic information content was *** sites were highly polymorphic(PIC>0.50).

关键词： microsatellite Acanthogobius ommaturus high-throughput sequencing polymorphic sites

Identification and Dna Barcoding of a New Sillago Species in Beihai and Zhanjiang,China,with a Key to Related Species

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Journal of Ocean University of China 2022年第5期21卷 1334-1342页

作者： YU Zhengsen GUO Ting XIAO Jiaguang song na GAO Tianxiang Fishery College Ocean University of ChinaQingdao 266003China Laboratory of Marine Biology and Ecology Third Institute of OceanographyMinistry of Natural ResourcesXiamen 361005China Fishery College Zhejiang Ocean UniversityZhoushan 316022China

A new Sillago species,Sillago parasihama sp.n.,is identified based on 127 specimens collected from the southern coast of *** compared the morphological characters between Sillago parasihama and all other 11 Sillago species with two posterior extensions on the swim *** new species is like *** in the countable characters and color pattern,but is different from the latter by the distinct swim *** swim bladder of *** is without lateral *** posterior sub-extensions of anterolateral extensions are unique with some dendritic or sometimes stunted blind tubule,which are unilateral and outward,ex-tending along the abdominal,and are about one-third to half of the body of swim bladder in *** the swim bladder of ***-hama with 8-10 lateral processes,the posterior sub-extensions of anterolateral extensions are kinky,long and complicated,extend-ing along the abdominal wall below the peritoneum to the base of posterior *** can be distinguished from other species in this group by color pattern,meristic,and morphometric ***,the results of genetic analysis using sequences of the mitochondrial cytochrome c oxidase subunit I(COI)fragment show significant interspecific-level genetic distances(0.159-0.231)between *** and 8 congeners in the group,which also support the validity of new *** also provide a distribution map and a key of the related species.

关键词： new species Sillago parasihama sp.n. morphological characteristics Dna barcoding taxonomy