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Recombinant amelogenin regulates the bioactivity of mouse cementoblasts in vitro

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International Journal of Oral science 2018年第2期10卷 117-126页

作者： sema s.Hakki s.Buket Bozkurt Emre Türkay Michel Dard Nuhan Purali Werner Gotz Department of Periodontology Faculty of DentistrySelcuk University Research Center of Dental Faculty Selcuk University Department of Orthodontics Oral Biology LaboratoryFaculty of DentistryUniversity of Bonn Department of Periodontology and Implant Dentistry College of DentistryNew York University Department of Biophysics Faculty of MedicineHacettepe University

Amelogenin（AMG） is a cell adhesion molecule that has an important role in the mineralization of enamel and regulates events during dental development and root formation. The purpose of the present study was to investigate the effects of recombinant human AMG（rhAMG） on mineralized tissue-associated genes in cementoblasts. Immortalized mouse cementoblasts（OCCM-30）were treated with different concentrations（0.1, 1, 10, 100, 1 000, 10 000, 100 000 ng · mL^-1） of recombinant human AMG（rhAMG）and analyzed for proliferation, mineralization and mRNA expression of bone sialoprotein（BsP）, osteocalcin（OCN）, collagen type I（COL I）, osteopontin（OPN）, runt-related transcription factor 2（Runx2）, cementum attachment protein（CAP）, and alkaline phosphatase（ALP） genes using quantitative RT-PCR. The dose response of rhAMG was evaluated using a real-time cell *** RNA was isolated on day 3, and cell mineralization was assessed using von Kossa staining on day 8. COL I, OPN and lysosomalassociated membrane protein-1（LAMP-1）, which is a cell surface binding site for amelogenin, were evaluated using immunocytochemistry. F-actin bundles were imaged using confocal microscopy. rhAMG at a concentration of 100 000 ng · mL^-1 increased cell proliferation after 72 h compared to the other concentrations and the untreated control group. rhAMG（100 000 ng · mL^-1） upregulated BsP and OCN mRNA expression levels eightfold and fivefold, respectively. rhAMG at a concentration of 100 000 ng · mL^-1 remarkably enhanced LAMP-1 staining in cementoblasts. Increased numbers of mineralized nodules were observed at concentrations of 10 000 and 100 000 ng · mL^-1 rhAMG. The present data suggest that rhAMG is a potent regulator of gene expression in cementoblasts and support the potential application of rhAMG in therapies aimed at fast regeneration of damaged periodontal tissue.

关键词： AMG Recombinant amelogenin regulates the bioactivity of mouse cementoblasts in vitro OCN

Quasi-in-situ observing the rare earth texture evolution in an extruded Mg-Zn-Gd alloy with bimodal microstructure

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Journal of Magnesium and Alloys 2021年第5期9卷 1818-1827页

作者： M.G.Jiang C.Xu H.Yan T.Nakata Z.W.Chen C.s.Lao R.s.Chen s.Kamado E.H.Han College of Mechatronics and Control Engineering Shenzhen University3688 Nanhai AveShenzhen 518060China The Group of Magnesium Alloys and Their Applications Institute of Metal ResearchChinese Academy of Sciences62 Wencui RoadShenyang 110016China school of Materials science and Engineering Harbin Institute of TechnologyHarbin 150001China Department of Mechanical Engineering Nagaoka University of TechnologyNagaoka 940-2188Japan

The static recrystallization and associated texture evolution were investigated in an extruded Mg-Zn-Gd alloy with bimodal microstructure based on a quasi-in-situ electron back-scatter diffraction(EBsD)*** typical rare earth(RE)texture formed during annealing,evolving from the bimodal microstructure with[1010]basal fiber texture that consisted of fine recrystallized(RXed)grains and coarse unrecrystallized(un RXed)grains elongated along the extrusion *** both RXed and un RXed regions,the RXed nucleation produced randomized orientations without preferred selection and the RXed grains with RE texture orientation had more intensive growth ability than those with basal fiber orientation,thereby leading to the preferred selection of RE texture orientation during grain *** relationships between stored strain energy,solute drag,grain growth and texture evolution are discussed in *** study provided direct evidence of the RE texture evolution in an extruded Mg-RE alloy,which assists in understanding the formation mechanisms for RE texture during extrusion and better developing wrought Mg alloys with improved formability.

关键词： Magnesium alloy Rare earth texture Extrusion Annealing Recrystallization

Forced convection heat transfer due to different inclination angles of splitter behind square cylinder

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Applied Mathematics and Mechanics(English Edition) 2013年第5期34卷 541-558页

作者： s.M.sEYYEDI D.D.GANJI M.GORJI H.BARARNIA s.sOLEIMANI Department of Mechanical Engineering Babol University of Technology

A numerical investigation is carried out to study the effect of splitter＇s inclination angle behind an inclined square cylinder on the forced convection heat transfer in a plan channel using the lattice Boltzmann method （LBM）. The simulations are conducted for the pertinent parameters in the following ranges： the Reynolds number Re=50-300, the gap ratio G/d = 2, and the splitter＇s inclination angle 8 = 0°-90°. The results show that with the increase in the angle of the splitter, the drag coefficient initially decreases and then increases. Moreover, the time-averaged Nusselt number at a certain angle increases noticeably.

关键词： s. M. sEYYEDI D.D. GANJI M. GORJI H. BARARNIA s. sOLEIMANI (forced convection inclined splitter square cylinder plan channel lattice Boltzmann method (LBM))

从历代文献探究蒙古书面语“元音+■”和“元音+■”的性质及其演变规律

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中国文字研究 2024年第1期 210-218页

作者：斯·劳格劳彻·萨如拉内蒙古大学蒙古学学院内蒙古呼和浩特010021

回鹘式蒙古文或现行蒙古文词中元音之后的■是音节、复合元音还是音节末辅音?学者们持有不同的观点。在蒙古文中,■是多音字形,因此单从蒙古文文献或上述字符自身所包含的信息很难判定其性质。本文以婆罗米文蒙古语文献、八思巴字蒙古... 详细信息

回鹘式蒙古文或现行蒙古文词中元音之后的■是音节、复合元音还是音节末辅音?学者们持有不同的观点。在蒙古文中,■是多音字形,因此单从蒙古文文献或上述字符自身所包含的信息很难判定其性质。本文以婆罗米文蒙古语文献、八思巴字蒙古语文献、托忒文文献等不同时期蒙古语文献为依据,从■所对应的字符序列阐释了其性质和演变规律。我们认为,在古代蒙古语词中元音之后的“■”表示一个单独的音节“yi”,但到中期蒙古语随着“y”的元音属性的逐渐突出,这一书写形式也发生了变化,词中依然是单独的音节,但词末变成了复合元音的组成部分。再到托忒文或现代蒙古文,词中和词末均变成了构成复合元音的元素。

关键词：蒙古书面语 “元音+■”形式 “元音+■”形式音节末辅音复合元音

Plethysmograph training:A refinement for collection of respiration data in mice

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动物模型与实验医学(英文) 2023年第4期6卷 369-374页

作者： Jorid B.sørli Karin s.Hougaard Niels Hadrup Chemical Work Environment The National Research Centre for the Working EnvironmentCopenhagenDenmark Department of Public Health University of CopenhagenCopenhagenDenmark Research Group for Risk-Benefit National Food InstituteTechnical University of DenmarkLyngbyDenmark

Inhaled chemicals can harm the *** effects can result in distinct changes in respiratory patterns;the type of change indicates where and how the respiratory system is ***,changes in respiratory patterns may be detected at much lower substance concentrations than those that cause more serious effects,such as histopathological *** in respiratory patterns can be studied experimentally by monitoring the breathing of mice placed in plethysmographs and exposing head-out to the test *** method is well established;however,it is not known if training mice in being restrained in the plethysmograph could increase the quality of data *** we report the results of training mice to be restrained in plethysmographs for 5 consecutive days,with respect to body weight,respiratory parameters,and time spent in the plethysmograph,before they are removed because of unstable breathing *** mice tolerated the procedure better(measured by time in the plethysmograph)on the second day of training than the first *** did not change the breathing parameters between *** parameters stabilized within 5 min after the mice were placed in the plethysmographs on all *** was an average of 3%weight loss between the first and last days of the training,indicating that the training procedure placed some strain on the *** reduces the number of mice attempting to escape from the plethysmograph.

关键词： Alarie assay refinement sensory irritation

MULTIPLE-sCATTERING DETERMINATION TO THE LOCAL ADsORPTION GEOMETRY OF sO_2/Pd(111)

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Acta Metallurgica sinica(English Letters) 2002年第1期15卷 81-86页

作者： s. Cao J.C. Tang P. Zhu L. Wang s.L shen Dept. of Phys. Zhejiang Univ. Hangzhou 310027 China

We have calculated the sulphur 1s near edge X-ray absorption fine structure (NEXAFs) spectra of sO2 adsorbed on Pd (111) in terms of the Multiple-scattering Cluster (MsC) method. The origin of the resonances in the NEXAFs spectra has been revealed. By the MsC calculation and a (DV)-Xα study we have for the first time identified two weak features existing in s K-edge NEXAFs of sO2/Pd(111) system, which are attributed to the interaction between the adsorbate and the substrate. Moreover, MsC calculation together with the reliability factor (R-factor) analysis are employed to determine the local adsorption structures. These results are broadly in agreement with those of Fourier-transform analysis of the surface extended X-ray absorption fine structure (sEXAFs).

关键词： Absorption Geometry Palladium scattering sulfur dioxide X ray spectroscopy

Comparative study of different theories on active earth pressure

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Journal of Central south University 2012年第10期19卷 2933-2939页

作者： s.P.Yap F.A.salman s.M.shirazi Department of Civil Engineering Faculty of EngineeringUniversity of MalayaKuala Lumpur 50603Malaysia

Determination of distribution and magnitude of active earth pressure is crucial in retaining wall designs. A number of analytical theories on active earth pressure were presented. Yet, there are limited studies on comparison between the theories. In this work, comparison between the theories with finite element analysis is done using the PLAXIs software. The comparative results show that in terms of distribution and magnitude of active earth pressure, RANKINE＇s theory possesses the highest match to the PLAXIs analysis. Parametric studies were also done to study the responses of active earth pressure distribution to varying parameters Increasing soil friction angle and wall friction causes decrease in active earth pressure. In contrast, active earth pressure increases with increasing soil unit weight and height of wall. RANK/NE＇s theory has the highest compatibility to finite element analysis among all theories, and utilization of this theory leads to proficient retaining wall design.

关键词： active earth pressure retaining wall PLAXIs comparative study RANKINE's theory COULOMB's theory

Classification of Electroencephalogram signals Using LsTM and sVM Based on Fast Walsh-Hadamard Transform

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Computers, Materials & Continua 2023年第6期75卷 5271-5286页

作者： saeed Mohsen sherif s.M.Ghoneim Mohammed s.Alzaidi Abdullah Alzahrani Ashraf Mohamed Ali Hassan Electronics and Communications Engineering Department Al-Madinah Higher Institute for Engineering and TechnologyGiza12947Egypt Department of Artificial Intelligence Engineering Faculty of Computer Science and EngineeringKing Salman International University(KSIU)South Sinai46511Egypt Department of Electrical Engineering College of EngineeringTaif UniversityTaif21944Saudi Arabia Department of Electronics and Communications Engineering October High Institute for Engineering and Technology6th of October CityGiza12596Egypt

Classification of electroencephalogram(EEG)signals for humans can be achieved via artificial intelligence(AI)***,the EEG signals associated with seizure epilepsy can be detected to distinguish between epileptic and non-epileptic *** this perspective,an automated AI technique with a digital processing method can be used to improve these *** paper proposes two classifiers:long short-term memory(LsTM)and support vector machine(sVM)for the classification of seizure and non-seizure EEG *** classifiers are applied to a public dataset,namely the University of Bonn,which consists of 2 classes–seizure and *** addition,a fast Walsh-Hadamard Transform(FWHT)technique is implemented to analyze the EEG signals within the recurrence space of the ***,Hadamard coefficients of the EEG signals are obtained via the ***,the FWHT is contributed to generate an efficient derivation of seizure EEG recordings from non-seizure EEG ***,a k-fold cross-validation technique is applied to validate the performance of the proposed *** LsTM classifier provides the best performance,with a testing accuracy of 99.00%.The training and testing loss rates for the LsTM are 0.0029 and 0.0602,respectively,while the weighted average precision,recall,and F1-score for the LsTM are 99.00%.The results of the sVM classifier in terms of accuracy,sensitivity,and specificity reached 91%,93.52%,and 91.3%,*** computational time consumed for the training of the LsTM and sVM is 2000 and 2500 s,*** results show that the LsTM classifier provides better performance than sVM in the classification of EEG ***,the proposed classifiers provide high classification accuracy compared to previously published classifiers.

关键词： Electroencephalogram LsTM sVM fast Walsh-Hadamard transform seizure accuracy sensitivity specificity

secure Audio Transmission Over Wireless Uncorrelated Rayleigh Fading Channel

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Computers, Materials & Continua 2022年第1期70卷 1603-1615页

作者： Osama s.Faragallah M.Farouk Hala s.El-sayed Mohsen A.M.El-bendary Department of Information Technology College of Computers and Information TechnologyTaif UniversityP.O.Box 11099Taif21944Saudi Arabia Department of Computer science and Engineering Faculty of Electronic EngineeringMenoufia UniversityMenouf32952Egypt Department of Electrical Engineering Faculty of EngineeringMenoufia UniversityShebin El-Kom32511Egypt Department of Electronics Technology Faculty of Technology and EducationHelwan UniversityCairoEgypt

Audio communications and computer networking play essential roles in our daily lives,including many domains with different *** in these technologies are *** consequence,there is a dire need to secure these technologies up to *** paper presents an efficient model for secure audio signal transmission over the wireless noisy uncorrelated Rayleigh fading ***,the performance of the utilized multiple secret keys-based audio cryptosystem is analyzed in different transformation *** discrete cosine transform(DCT),the discrete sine transform(DsT),and the discrete wavelet transform(DWT)are investigated in the utilizedmultiple secret key-based audio *** results show consistent results with the wireless noisy *** performance of the proposed multiple secret keys-based audio cryptosystem can be ranked concerning the employed domain as DWT,DCT,and DsT transform *** simulation experiments proved that the presented multiple secret keysbased audio cryptosystemfor audio signals transmitted over the wireless noisy uncorrelatedRayleigh fading channel achieves reliable and secure wireless link utilizing combined multi security layers.

关键词： Audio communications encryption uncorrelated Rayleigh fading channel