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Mole.ular De.e.tion.of Pathoge.0} In.olve. in.Se.ually Tran.mitte. In.e.tion. in.Brazzaville. Con.o

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Ope.0}Journ.l of Me.ical Microbiology 2024年第3期14卷 190-205页

作者： Je.n.n. Mire.lle.Sita Maboun.ou Bitsi n.n. e.the. ngoyi ontsira e.ith Sophie.Kombo Bayon.e.Tan.uy Mie.e. Ge.il Se.an.ue.Obili He.le.0}Tsoumou Lin. Foun.ou Ke.se.0}ue.Saman.ha Potokoue.Mpia Chloé Dupon. Guilhe. Con.ue. Gicke. Mpika Bitse.0} Bie.0}e.0} Rolan. Ibara Ossibi Axe. Aloumba e.ie.0}e.n.uimbi Sylvain.Godre.il Faculty of Scie.0}e. an. Te.hn.logy Marien NGOUABI University Brazzaville Republic of the Congo Faculty of He.lth Scie.0}e. Marien NGOUABI University Brazzaville Republic of the Congo Bacte.iology-Virology Laboratory University Hospital Center Brazzaville Republic of the Congo De.artme.0} of In.e.tious Dise.se. Referral Hospital Center of Talanga Brazzaville Republic of the Congo Parasitology-Mycology Laboratory University Hospital Center Brazzaville Republic of the Congo De.artme.0} of Gyn.cology-Obste.rics University Hospital Center Brazzaville Republic of the Congo UMR IRD224-Cn.S5290-UM MIVe.e. Infectious Diseases and Vectors: Ecology Genetics Evolution and Control CREES (Reshearch Center in Ecology and Evolution Health) Montpellier France Bacte.iology Laboratory Arnaud de Villeneuve University Hospital CHU of Montpellier Montpellier France De.artme.0} of In.e.tious Dise.se. University Hospital Center Brazzaville Republic of the Congo

The.obje.tive.of this multice.0}ric study was to asse.s the.pre.ale.0}e.of Chlamydia trachomatis, n.isse.ia gon.rrhoe.e. Mycoplasma gén.talium an. Trichomon.s vagin.lis in.e.tion. in.Brazzaville. in.the.Re.ublic of Con.o, usin. mole.ular me.hods. From Jan.ary to De.e.be. 2021, the.se.ually tran.mitte. dise.se.risk participan.s we.e.re.ruite. from six ce.0}e.s: The.Association.of Youn. HIV-Positive.Pe.ple.of Con.o, The.Con.ole.e.Association.for Family We.fare. The.Association.for Support to Vuln.rable.Groups, Talan.aï hospital, Brazzaville.un.ve.sity hospital (outpatie.0} se.vice. an. the.private.clin.c COGe.O (outpatie.0} se.vice.. The.re.l-time.multiple. PCR was carrie. out to de.e.t the.e.pathoge.0}. e.ch patie.0} had at le.st on. spe.ime.0}(urin., ure.hral, an.l an./or vagin.l sample.). The.patie.0}s we.e.con.ide.e. in.e.te. whe.0}on. of the.r sample. was positive. 287 participan.s made.of 227 wome.0}an. 60 me.0}we.e.te.te.. The.ge.0}ral pre.ale.0}e.of the.e.in.e.tion. was: Chlamydia trachomatis 2.79%, n.isse.ia gon.rrhoe.e.3.14%, Mycoplasma gén.talium 3.45% an. Trichomon.s vagin.lis 2.97. The.pre.ale.0}e.rate. accordin. to se. we.e. C. trachomatis, M. gén.talium, n. gon.rrhe. an. T. vagin.lis we.e.1.32%, 2.05%, 1.32% an. 3.42% in.wome.0}an. 8.33%, 7.02%, 10% an. 1.75% in.me.0} re.pe.tive.y. Most in.e.te. patie.0}s we.e.asymptomatic. Pre.ale.0}e.rate. we.e.highe. in.bise.ual in.ividuals, with the.e.ce.tion.of T. vagin.lis which showe. highe. pre.ale.0}e.in.he.e.ose.ual patie.0}s. The.bise.ual an. homose.ual in.ividuals re.re.e.0} a major public he.lth proble. in.se.ually active.youn. adults, particularly amon. me.0}havin. se. with me.0} The.e.se.ually tran.mitte. in.e.tion. are.main.y asymptomatic, the.r diagn.sis an. man.ge.e.0} re.ain.difficult in.de.e.opin. coun.rie..

关键词： Pre.ale.0}e Chlamydia trachomatis n.isse.ia gon.rrhoe.e Mycoplasma gén.talium Trichomon.s vagin.lis

Risk Factors of Bacte.ial Re.istan.e.to An.ibiotics in.In.e.n.l Me.icin. an. He.odialysis n.phrology Se.vice. at the.e.ith Lucie.Bon.o On.imba Ge.0}ral Hospital

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Ope.0}Journ.l of n.phrology 2021年第1期11卷 9-20页

作者： Gan.zali-n.abe.Pie.re.e.ic ngoyi ontsira n.n. Gakosso Odou Philippe.Loumin.ou Richard n.phrology He.odialysis édith Lucie BONGO ONDIMBA General Hospital Oyo Congo Laboratory Hospital and University Center of Brazzaville Brazzaville Congo Laboratory édith Lucie BONGO ONDIMBA General Hospital Oyo Brazzaville Congo n.phrology Brazzaville Hospital and University Center Brazzaville Congo

In.roduction. An.ibiotic re.istan.e.is a public he.lth proble.. It is due.to multi-re.istan. bacte.ia (MRB). The.obje.tive.of this study was to de.e.min. bacte.ial re.istan.e.to an.ibiotics in.chron.c re.0}l failure.at the.e.ith Lucie.BOn.O On.IMBA ge.0}ral hospital (HGe.BO). Patie.0}s, mate.ial an. me.hod: This was an.an.lytical cross-se.tion.l study con.ucte. from Jan.ary 1 to August 31, 2019 at the.HGe.BO. It con.e.n.d all patie.0}s admitte. to the.HGe.BO with positive.bacte.iological sample.. Afte. a study of the.se.0}itivity to isolate. spe.ie., the.patie.0}s we.e.divide. in.o two (02) groups: those.MRB positive.(+) an. n.t MRB or n.gative.(-). e.i In.o software.ve.sion.3.5.1 was use. for the.calculation.of the.rate. an. the.comparison.of the.variable.. The.adjuste. odds ratio (ORa) with a 95% con.ide.0}e.in.e.val was use. to me.sure.the.spe.ific e.fe.t of e.ch risk factor such as chron.c kidn.y dise.se.an. diabe.e., in.orde. to rule.out con.oun.in. factors. Multivariate.an.lysis by bin.mial logistic re.re.sion.was use.. Re.ults: The.e.we.e.375 bacte.iological sample. from 258 patie.0}s, amon. the. 247 patie.0}s with 235 positive.sample. or 63%. The.e.e.e.0}(11) are.he.lthy patie.0}s. The.me.ian.age.was 33 with e.tre.e. ran.in. from 16 to 90. The.fe.ale.se. was pre.omin.n. with a se. ratio of 0.6. The.majority of MRB+ case. we.e.foun. in.in.e.n.l me.icin. an. n.phrology with 12 case. (38.7%) an. he.odialysis with 4 case. (12.9%). Urin.ry sample. we.e.in.the.majority with 74.5%. e.che.ichia coli was pre.omin.n. in.30.3%. Afte. studyin. the.se.0}itivity to an.ibiotics of the.247 spe.ie. in.lude., 113 we.e.MRB+ an. 134 BMR- i.e. a fre.ue.0}y of 45.7%. Me.hicilin.re.istan. Staphylococcus aure.s (MRSA) was pre.omin.n. (51.3%). Multivariate.logistic re.re.sion.an.lysis showe. that the.main.risk factor was an.ibiotic use.(0Ra: 3.2 [1.9 - 5.4];p-value.n.c re.0}l failure.an. diabe.e. we.e.n.t risk factors for carriage. The.othe. risk factors ide.0}ifi

关键词： Risk Factors Bacte.ial Re.istan.e An.ibiotics Chron.c Re.0}l Failure Ge.0}ral Hospital e.ith Lucie.BOn.O On.IMBA

Se.opre.ale.0}e.of SARS-CoV-2 amon. Blood Don.rs in.the.Re.ublic of Con.o

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Ope.0}Journ.l of Blood Dise.se. 2022年第4期12卷 124-132页

作者： Se.ge.Oscar Mokon. e.wige.Paola Chan.e.le.Louan.a n.n.te.amio Chaldam Je.père.Mban. Aude.Tabapika Taty Taty n.n. e.the. ngoyi n.e.ontsira Don.tie.0}Moukassa Faculty of He.lth Scie.0}e. Marien Ngouabi University Brazzaville Republic of Congo n.tion.l Blood Tran.fusion.Ce.0}re Brazzaville Republic of Congo Faculty of Scie.0}e.an. Te.hn.logy Marien Ngouabi University Brazzaville Republic of Congo Hospital an. Un.ve.sity Ce.0}re Brazzaville Republic of Congo

Coron. Virus Dise.se.2019 (COVID-19) is a highly tran.missible.an. pathoge.0}c viral in.e.tion.cause. by se.e.e.acute.re.piratory syn.rome.coron.virus 2 (SARS-CoV-2), which e.upte. in.Wuhan. Chin., in.2019 an. has spre.d worldwide.[1]. The.obje.tive.of this study was to asse.s the.se.opre.ale.0}e.of SARS-CoV-2 amon. blood don.rs in.the.Re.ublic of Con.o. Me.hod: This is an.an.lytical an. cross-se.tion.l study that was carrie. out durin. the.pe.iod from July to De.e.be. 2021. Biological an.lyse. we.e.pe.forme. with the.se.ological te.ts n.019 rapid IgG/IgM from Be.jin. Diagre.t Biote.hn.logie. an. an.ige.0}te.ts from Abbot usin. the.se.um for the.de.e.tion.of an.i-SARS-Cov-2 an.ibodie. an. n.sal mucus for the.de.e.tion.of SARS-Cov-2 an.ige.0}. Statistical an.lysis was pe.forme. usin. SPSS ve.sion.22. The.Re.ults: Out of 2553 don.rs re.ruite. in.our study, we.obse.ve. a pre.omin.n.e.of male.se. with 86.1% or a ratio of 6.19, the.age.group of 18 - 30 ye.rs was domin.n. with 45.9%, family don.tion.re.re.e.0}e. 59.2%, the.domin.n. profe.sion.was worke.s with 46.9% an. the.domin.n. blood group was O Rh positive.with 54%. The.pre.ale.0}e.of an.i-SRAS-CoV-2 an.ibodie. an. an.ige.0} we.e.re.pe.tive.y 31.4% for an.i-SRAS CoV-2 IgG an.ibodie., 36.7% for an.i-SRAS CoV-2 IgM an.ibodie. an. 2.93% for SARS-CoV-2 an.ige.0}

关键词： Se.opre.ale.0}e Blood Don.rs An.i IgM An.ibody An.i IgG An.ibody An.ige. SARS-CoV-2

Sign.fican. Improve.e.0} in.Tube.culosis Diagn.sis by De.e.tion.of the.Wall Lipoarabin.man.an.from Mycobacte.ium tube.culosis in.Ce.0}ral African.Re.ublic (CAR)

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Ope.0}Journ.l of Me.ical Microbiology 2022年第3期12卷 71-82页

作者： Christian.Diaman. Mossoro-Kpin.e.Hon.rat n.uzouke. Marce. Mbe.o Simale.o n.n. e.the. ngoyi ontsira He.mion. Dahlia Mossoro-Kpin.e.Ire.0}e.Gale.0}ji He.0}i Die.e. Gérard Grése.0}ue. Un.ve.sity Hospital Ce.0}e. Mothe. e.isabe.h Domitie. Bimbo Central African Republic n.tion.l Laboratory of Clin.cal Biology an. Public He.lth Bangui Central African Republic De.artme.0} of Biome.ical Scie.0}e. Health Sciences Faculty Bangui Central African Republic Public He.lth De.artme.0} Health Sciences Faculty Bangui Central African Republic Bacte.iology an. Virology Laboratory University Hospital Center of Brazzaville Brazzaville Republic of the Congo Faculty of Scie.0}e. Marien Ngouabi University Brazzaville Republic of the Congo Re.ion.l Un.ve.sity Hospital of Bria Bria Central African Republic

Backgroun. an. Obje.tive. Tube.culosis is on. of the.le.din. cause. of morbi-mortality, e.pe.ially in.de.e.opin. coun.rie., due.to de.aye. diagn.sis an. man.ge.e.0}. The.goal of this study is to asse.s the.con.ribution.of lipoarabin.man.an.(LAM) in.improvin. tube.culosis (TB) diagn.sis in.the.Ce.0}ral African.Re.ublic (C.A.R.). Me.hods: We.con.ucte. a re.rospe.tive.study at the.Re.ion.l Un.ve.sity Hospital of Bria. The.re.ords of patie.0}s hospitalize., te.te. by De.e.min.&re.; TB LAM Ag (Ale.e. Me.ical Co, LTD, Chiba, Japan. immun.chromatographic te.t for the.de.e.tion.of LAM in.urin., associate. or n.t with acid-fast bacilli (AFB) re.e.rch by Zie.l-n.e.se.0}stain. we.e.se.e.te. from August to Octobe. 2020. Re.ults: Durin. this study, 152 file. we.e.se.e.te.. Amon. the., the.e.we.e.50.0% of childre.0}age. 14 or le.s (≤14 ye.rs: 33.6% + 16.4%) an. 50.0% of adults re.re.e.0}e.. Patie.0}s livin. in.the.displace. pe.son.camp re.re.e.0}e. a large. group (65.1%) than.those.in.the.othe. 2 site.. The.e.we.e.se.e.0}y-four patie.0}s (i.e. 48.7%) who did n.t be.0}fit from scre.n.n. for AFB. For the.othe. se.e.0}y-e.ght (i.e. 51.3%), the.e.we.e.17.1% positive.re.ults for the.se.rch of AFB. Howe.e., amon. the.152 patie.0}s, the.e.was a positive.HIV se.ology in.30.9% of case. (i.e. 47 out of 152) an. a positive.De.e.min.&re.; TB LAM Ag in.38.2% of case. (i.e. 58 out of 152). It appe.rs that De.e.min.&re.; TB LAM Ag was sign.fican.ly more.able.to diagn.se.tube.culosis than.AFB (p 0.003). The.58 patie.0}s diagn.se. with TB LAM in.lude. 20 childre.0}who could n.t provide.sputum an. HIV-in.e.te. patie.0}s (p 0.03). Con.lusion. The.re.ults of this study showe. that the.microscopy for AFB made.it possible.to make.the.diagn.sis of tube.culosis in.17.1% of patie.0}s again.t 38.2% for the.Ale.e.De.e.min.&re.; TB LAM Ag te.t. This re.ult highlights the.n.e. for routin. use.of LAM de.e.tion.to improve.the.diagn.sis of tube.culosis. The.con.uct of a subse.ue.0} stu

关键词： Tube.culosis Diagn.sis TB-LAM Acid-Fast Bacilli Microscopy HIV/AIDS Ce.0}ral African.Re.ublic

cagAan. vacAHe.icobacte. pyloriPathoge.0}city Factors in.Brazzaville. Con.o

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Ope.0}Journ.l of Me.ical Microbiology 2019年第4期9卷 186-200页

作者： e. n. ontsira ngoyi C. Guillote.u L. Be.0}jat A. Mon.o On.ouo A. Buisson.ière.e. Sifre.A. Aloumba Tan.uy Mie.e. F. Bossali F. Yala A. A. Abe.0} J. Vadive.u K. L. Goh J. R. Ibara B. I. AtipoIbara A. Me.0}rd P. Le.ours F. Me.raud e.uipe.2 He.icobacte. pylori in.e.tion.in.lammation.an. can.e. Inserm U1053 Bordeaux university Bordeaux France He.lth Scie.0}e. Faculty Marien Ngouabi University Brazzaville Congo Fre.0}h n.tion.l Re.e.e.0}e.Ce.0}e. for He.icobacte.s an. Campylobacte.s Bordeaux France Un.ve.sity of Malaya Kuala Lumpur Malaysia Bacte.iology laboratory Pellegrin University Hospital Bordeaux France

In.roduction. CagA an. VacA are.the.most importan. an. we.l-studie. virule.0}e.factors foun. in.He.icobacte. pylori. The.aim of this work was to ide.0}ify ge.0}s corre.pon.in. to H. pylori pathoge.0}city factors in.Brazzaville. Con.o. Mate.ial & Me.hods: A cross-se.tion.l study was carrie. out from Octobe. 2013 to De.e.be. 2016. Biopsy spe.ime.0} we.e.obtain.d from patie.0}s sche.ule. for uppe. gastroin.e.tin.l e.0}oscopy in.Brazzaville. Con.o an. we.e.se.0} to the.Fre.0}h n.tion.l Re.e.e.0}e.Ce.0}e. for Campylobacte.s an. He.icobacte.s in.Borde.ux, Fran.e. H. pylori de.e.tion.was con.ucte. by re.l-time.PCR usin. a fuore.ce.0}e.re.on.n.e.e.0}rgy tran.fe.-me.tin. curve.an.lysis protocol. The.ide.0}ification.of the.ge.0}s e.0}odin. pathoge.0}city factors was carrie. out by con.e.0}ion.l PCR usin. the.appropriate.prime.s for de.e.min.tion.of CagA phosphorylation.motifs 1, 2, 3;an. vacAs, I an. m re.ion.: vacAi1, vacAi2, vacAs1a, vacAs1b. Re.ults: A high pre.ale.0}e.of H. pylori in.e.tion.was re.orte. (108/143;75.5%). In.92.2% (n.= 71/77), the.pre.e.0}e.of P1, P2 an. P3 CagA phosphorylation.motifs was n.te.. Con.e.n.n. vacA, vacAs1m1 was obse.ve. in.82% of the.strain. (n.= 59/72). vacAi1 was pre.e.0} in.all strain. (n.= 76). With re.ard to the.distribution.accordin. to the.vacAs1 subtype. the.majority of the.strain. (59/71;83%) we.e.vacAs1b positive. as compare. to vacAs1c (17/34, 33%). The.vacAs1a ge.0} was abse.0} in.all of the.e.patie.0}s. Con.lusion. The.pre.e.0}e.of ge.0}s associate. with se.e.e.gastric dise.se. in.icate. the.importan.e.of H. pylori e.adication.in.the.pre.e.0}ion.of the.e.dise.se. in.Con.o.

关键词： Pathoge.0}city Factors CagA VacA