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General Regulatory Patterns of Plant Mineral Nutrient Depletion as Revealed by serat Quadruple Mutants Disturbed in Cysteine Synthesis

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Molecular Plant 2010年第2期3卷 438-466页

作者： mutsumi watanabe Hans-Michael Hubberten Kazuki Saito Rainer Hoefgen Graduate School of Pharmaceutical Sciences Chiba University 1-33 Yayoi-cho Inage-ku Chiba 263-8522 Japan Max-Planck-lnstitut fuer Molekulare Pflanzenphysiologie Wissenschaftspark Golm Am Muehlenberg 1 14476 Potsdam-Golm RIKEN Plant Science Center Tsurumi-ku Yokohama 230-0045 Japan

Sulfate is an essential macronutrient for plants. Plants have developed strategies to cope with sulfate deficiency, and other nutrient ion limitations. However, the regulation of these adaptive responses and the coordinating signals that underlie them are still poorly characterized. O-acetylserine （OAS） is a marker metabolite of sulfate starvation and has been speculated to have a signaling function. OAS is synthesized by the enzyme serine acetyltransferase （SERAT）, which is encoded by five distinct genes in Arabidopsis. We investigated quadruple knockout mutants of SERAT that retained only one functional isoform. These mutants displayed symptoms of sulfate starvation. Furthermore, some of them displayed phenotypes typical of prolonged sulfate starvation, in particular, developmental programs associated with senescence or stress responses. Thus, we compared metabolite and transcriptome data from these mutants with N-, P-, K-, and S-depleted plants. This revealed many similarities with general nutrient-depletion-induced senescence （NuDIS）, in- dicating the recruitment of existing regulatory programs for nutrient-starvation responses. Several candidate genes that could be involved in these processes were identified, including transcription factors and other regulatory proteins, as well as the functional categories of their target genes. These results outline components of the regulatory network controlling plant development under sulfate stress, forming a basis for further investigations to elucidate the complete network. In turn, this will advance our broader understanding of plant responses to a range of other nutrient stresses.

关键词： Abiotic/environmental stress ion/metabolite sensing senescence functional genomics Arabidopsis sulfur.

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Exploiting Natural Variation in Tomato to Define Pathway Structure and Metabolic Regulation of Fruit Polyphenolics in the Lycopersicum Complex

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Molecular Plant 2020年第7期13卷 1027-1046页

作者： Takayuki Tohge Federico Scossa Regina Wendenburg Pierre Frasse llse Balbo mutsumi watanabe Saleh Alseekh Sagar Sudam Jadhav Jay C.Delfin Marc Lohse Patrick Giavalisco Bjoern Usadel Youjun Zhang Jie Luo Mondher Bouzayen Alisdair R.Fernie Max-Planck-lnstitut fiir Molekulare Pflanzenphysiologie Am Muehlenberg 114476 Potsdam-Golm.Germany Graduate School of Biological Science Nara Institute of Science and TechnologyIkoma 630-0192 Japan Council for Agricultural Research and Economics(CREA) Research Centre for Genomics and Bioinformaticsvia Ardeatina 54600178 RomeItaly Universite de Toulouse INP-ENSA ToulouseGenomique et Biotechnologie des FruitsCastanet-Tolosan 31326France Institute of Plant Systems Biology 4000 PlovdivBulgaria Max Planck Institute for Biology of Ageing Joseph Stelzmann Strasse 9b50931 CologneGermany Institute of Botany and Molecular Genetics BioSCRWTH Aachen University52056 AachenGermany National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(Wuhan) Huazhong Agricultural UniversityWuhan 430070China

While the structures of plant primary metabolic pathways are generally well defined and highly conserved across species,those defining specialized metabolism are less well characterized and more highly variable across *** this study,we investigated polyphenolic metabolism in the lycopersicum complex by characterizing the underlying biosynthetic and decorative reactions that constitute the metabolic network of polyphenols across eight different species of *** this purpose,GC-MS-and LC-MS-based metabolomics of different tissues of Solatium lycopersicum and wild tomato species were carried out,in concert with the evaluation of cross-hybridized microarray data for MapMan-based transcriptomic analysis,and publicly available RNA-sequencing data for annotation of biosynthetic *** combined data were used to compile species-specific metabolic networks of polyphenolic metabolism,allowing the establishment of an entire pan-species biosynthetic framework as well as annotation of the functions of decoration enzymes involved in the formation of metabolic diversity of the flavonoid *** combined results are discussed in the context of the current understanding of tomato flavonol biosynthesis as well as a global view of metabolic shifts during fruit *** results provide an example as to how large-scale biology approaches can be used for the definition and refinement of large specialized metabolism pathways.

关键词： Solanumly copersicum secondary metabolism natural diversity wild accessions pathway elucidation gene discovery

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自动心内膜轮廓跟踪技术

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当代医学 2002年第1期8卷 67-68页

作者： Mashide Nishiura mutsumi watanabe 东芝株式会社三厂医疗超声产品部

东芝公司最新开发了一种超声心动图心内膜描记技术自动心内膜轮廓跟踪(ACT).众所周知,缺血性心脏病人的心肌运动不协调,心脏泵功能减退.

关键词：自动心内膜轮廓跟踪原理应用心功能

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Non-aqueous fractionation revealed changing subcellular metabolite distribution during apple fruit development

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Horticulture Research 2019年第1期6卷 556-567页

作者： Wasiye F.Beshir Takayuki Tohge mutsumi watanabe Maarten L.A.TMHertog Rainer Hoefgen Alisdair R.Fernie Bart M.Nicolaï Division of Mechatronics Biostatistics and Sensors(MeBioS)Department of Biosystems(BIOSYST)KU LeuvenLeuvenBelgium Max Planck Institute of Molecular Plant Physiology(MPI-MP) Potsdam-GolmGermany Flanders Centre of Postharvest Technology(VCBT) LeuvenBelgium

In developing apple fruit,metabolic compartmentation is poorly understood due to the lack of experimental *** subcellular compartments in fruit using non-aqueous fractionation has been technically difficult due to the excess amount of sugars present in the different subcellular compartments limiting the resolution of the *** work described in this study represents the first attempt to apply non-aqueous fractionation to developing apple fruit,covering the major events occurring during fruit development(cell division,cell expansion,and maturation).Here we describe the non-aqueous fractionation method to study the subcellular compartmentation of metabolites during apple fruit development considering three main cellular compartments(cytosol,plastids,and vacuole).Evidence is presented that most of the sugars and organic acids were predominantly located in the vacuole,whereas some of the amino acids were distributed between the cytosol and the *** results showed a shift in the plastid marker from the lightest fractions in the early growth stage to the dense fractions in the later fruit growth *** implies that the accumulation of starch content with progressing fruit development substantially influenced the distribution of plastidial fragments within the non-aqueous density gradient *** from this study provide substantial baseline information on assessing the subcellular compartmentation of metabolites in apple fruit in general and during fruit growth in particular.

关键词： technique distribution sugar

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