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Antimicrobial Property of 2-Hydroxypropane-1,2,3-Tricarboxylic Acid Isolated from Citrus microcarpa Extract

Agricultural Sciences in China 2009年第7期8卷 880-886页

作者： Seong Wei Lee musa najiah Department of Fishery and Aquaculture Faculty of Agrotechnology and Food Science Universiti Malaysia Terengganu

This article described antimicrobial property and structure analysis of 2-hydroxypropane-1,2,3-tricarboxylic acid isolated from the crude extract of Citrus microcarpa. Presently, there was no report on compound from C. microcarpa that possessed antimicrobial property against fish pathogenic bacteria. Therefore, in this study, the bioactive principle in C. microcarpa extract was isolated using thin layer chromatography. It＇s structure was elucidated based on nuclear magnetic resonance （NMR） spectroscopic data, such as proton NMR （1HNMR）, correlation spectroscopy, carbon 13 NMR, and heteronuclear multiple bond correlation data. This study showed that the bioactive compound isolated from C. microcarpa was 2-hydroxypropane-1,2,3-tricarboxylic acid monohydrate. The minimum inhibitory concentration （MIC） values of crude C. microcarpa extract and its bioactive component, 2-hydroxypropane-1,2,3-tricarboxylic acid as well as commercially available synthetic 2-hydroxypropane-1,2,3-tricarboxylic acid, were determined against 18 isolates of Edwardsiella tarda and 7 bacterial reference strains, namely, Escherichia coli （ATCC 25922）, Citrobacter freundii （ATCC 8090）, Aeromonas hydrophila （ATCC 49140）, Pseudomonas aeruginosa （ATCC 35032）, Streptococcus agalatiae （ATCC 13813）, E. tarda （ATCC 15947）, and Yersinia enterocolitica （ATCC 23715）, using two-fold microdilution method. The MIC values for both the natural 2-hydroxypropane-1,2,3-tricarboxylic acid and the synthetic one were ranging from 15.6 to 62.5 mg mL-1, whereas that of the crude extract was ranging from 7.8 to 31.3 mg mL-1. These findings showed that both the crude extract and its bioactive component might have potential as antimicrobial agent for aquaculture use.

关键词： antimicrobial property 2-hydroxypropane-1,2,3-tricarboxylic acid Citrus microcarpa NMR MIC

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Bacterial diseases outbreak of African Catfish （Clarias gariepinus） from Manir River, Tcrengganu, Malaysia

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Journal of Life Sciences 2009年第5期3卷 10-13,20页

作者： najiah musa LEE Seong Wei WENDY Wee NADIRAH musa Department of Fisheries and Aquaculture Faculty of Agroteehnology and Food Science Universiti Malaysia Terengganu （UMT） Terengganu 21030 Malaysia

A total of 190 bacterial isolates have been successfully isolated from 50 diseased African Catfish （C/arias gariepinus） namely; 70 isolates of Flavobacterium sp., 50 isolates of Aeromonas hydrophila, 30 isolates of Edwardsiella tarda and 20 isolate of each Hafnia alvei and Pseudomonas aeruginosa. This study was conducted to investigate bacteria that infected the broodstocks of African catfish cultured at Manir River, Terengganu as well as their antibiogram and Multiple Antibiotic Resistance （MAR） index. In the present study, antibiotic sensitivity and intermediary case were reported as 52.7% and 7.8%, respectively. Meanwhile, antibiotic case was recorded as 34.5%. The MAR index values obtained by each bacterial species was ranging from 0.24 to 0.52 whereas the average MAR index value was 0.39 which indicated that the cultured fish has been highly exposed to tested antibiotics.

关键词： MAR index Clarias gariepinus African catfish Broodstock

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Identification of Chitosan-Degrading Microbes for the Production of Chitooligomer

Identification of Chitosan-Degrading Microbes for the Produc...

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2012 3rd International Conference on Chemistry and Chemical Engineering

作者： Mat B Zakaria Syaidatul najiah Zakaria Muslihah musa Hasrani Hamilin Nurul Asma Hasliza Zulkifly Department of Biotechnology Faculty of Agriculture and BiotechnologyUniversiti Sultan Zainal AbidinGong Badak Campus21300 Kuala TerengganuTerengganuMALAYSIA

A total of twenty bacteria were isolated from various kinds of seawater and soil samples collected along the beach of Kuala *** minimal media and solid minimal media were used for enrichment and direct plating isolation,*** strains were isolated based on their ability to grow in liquid minimal media supplemented with 1% chitosan powder as the sole carbon and energy *** 20 selected bacterial strains,only 10 cell cultures showed signs of inference of chitosan *** activity of which was measured quantitatively for the reducing sugar produced in the growth *** strain isolated from Batu Buruk beach （L1） and from Chendering beach （BB2） showed a promising extent of biodegradation with the released reducing sugar concentration of 0.727 and 0.572 μmol/mL,respectively.

关键词： Chitosan chitosanase Chitosanase-producing microorganisms D-glucosamine N-acetyl-D-glucosamine

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