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DNA Extraction from Insects by Using Different Techniques: A Review

Advances in Entomology 2015年第4期3卷 132-138页

作者： Usman Asghar muhammad faheem malik Fakhra Anwar Ayesha Javed Ali Raza Department of Zoology University of Gujrat Hafiz Hayat Campus Gujrat Pakistan

This review paper was prepared at Department of Zoology, University of Gujrat, Pakistan during 2014-2015. The data for the two decades, DNA Extraction from Insects by Using Different Techniques was compiled through a thorough review of many research articles published in various journals of international repute. Insects are very important economically and ecologically for human due to their various roles, as pests, decomposer in nutrition cycle, vector for the transmission of certain diseases and a source of medically and economically important products. To identify insect, and for research on their role, different molecular techniques are used. For each molecular technique, high quality DNA is required, which is extracted from the insects by different techniques. The selection of DNA extraction technique is depended upon specimen under study, time required for extraction, economical stander of technique due to reagents and equipment used for extraction and most importantly extracted DNA quality. In this paper, most frequently used techniques for DNA extraction from insect with different size and condition are outlined. This paper is only a guide for these techniques and we describe them briefly.

关键词： Extraction Techniques Reagents

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Introduction, Sign, Symptoms, Prevention and Management of Lyme Disease Caused by Borrelia burgdorferi Channeled through Ixodes Ticks as Vector. A Review

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Advances in Entomology 2016年第5期4卷 249-259页

作者： Fakhra Tabbasam muhammad faheem malik Usman Asghar Kamaran Saeed Paracha Takhmina Nazir University of Gujrat Ghakkhar Pakistan

God has created many miraculous creature;ticks are one of them. Borrelia burgdorferi is a bacterium that lives into the gut of ticks. When such ticks feed upon the blood of Ticks and other livestock, bacteria are transferred into their blood stream of these hosts. A disease is caused into human beings if such infected tick bites humans that are called as Lyme disease. This review will help to have a keen study on the cause, sign, symptoms, prevention and management of this disease.

关键词： Borrelia burgdorferi Livestocks

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Homeobox leucine zipper proteins and cotton improvement

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Advances in Bioscience and Biotechnology 2013年第10期4卷 15-20页

作者： Muzna Zahur muhammad Ahsan Asif Nadia Zeeshan Sajid Mehmood muhammad faheem malik Abdul R. Asif Department of Biochemistry and Molecular Biology University of Gujrat Gujrat Pakistan Department of Clinical Chemistry University Medical Center Goettingen Goettingen Germany

Transcription factors play key roles in plant development and stress responses through their interaction with cis-elements and/or other transcription factors. Homeodomain associated leucine zipper proteins (HD-Zip) constitute a family of transcription factors that are characterized by the presence of a DNA-binding domain closely linked with leucine zipper motif functioning in dimer formation. This type of association is unique to plants and considered as an excellent candidate to activate developmental responses to altering environmental conditions. Cotton is the most important fiber plant with a lot of local and commercial uses in the world. HD-Zip proteins not only have key roles in different stages of vascular and inter-fascicular fiber differentiation of cotton but also are suggested to have an important role against abiotic stress that is one of the key factors limiting cotton productivity. Plants have developed various strategies to manage stress conditions through a combination of metabolic, physiological and morphological adaptations. These adaptive changes rely largely on alterations in gene expression. Therefore, transcriptional regulators play a crucial role in stress tolerance. Being a transcription factor HD-Zip might be a useful target for genetic engineering to generate multiple stress tolerance in susceptible plants. In the following chapter, we discussed how the HD-Zip proteins would play a useful role for cotton development both in fiber production and stress adaptation.

关键词： Cotton Stress Transcription Factor HD-Zip Proteins Homeobox Leucine Zipper

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