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HD-Zip Transcription Factor is Responsible for No-Lobed Leaf in Watermelon(Citrullus lanatus L.)

Phyton-International Journal of Experimental Botany 2023年第5期92卷 1311-1328页

作者： Shixiang Duan Yaomiao Guo Yinping Wang muhammad Jawad umer Dongming Liu Sen Yang Huanhuan Niu Shouru Sun Luming Yang Junling Dou Huayu Zhu College of Horticulture Henan Agricultural UniversityZhengzhou450002China Cotton Institute Chinese Academy of Agricultural SciencesAnyang455000China

Leaf is a vital organ of plants that plays an essential role in photosynthesis and *** an important agronomic trait in leaf development,leaf shape is classified into lobed,entire(no-lobed),and serrated in most *** this study,two-lobed leaf watermelon inbred lines WT2 and WCZ,and a no-lobed leaf watermelon inbred line WT20 were used to create two F_(2)*** analysis suggested that lobed leaves were dominant over the no-lobed leaves,and it was controlled by a signal gene.A locus on watermelon chromosome 4 controlling watermelon lobed/no-lobed leaves was identified through BSA-seq strategy combined with linkage *** candidate gene was fine-mapped to a 61.5 kb region between 21,224,481 and 21,285,957 bp on watermelon chromosome 4 using two F_(2)*** functional genes were annotated in the candidate region,while sequences blast showed that there was a single-base deletion(A/-)only in the exon of Cla018360,which resulted in premature termination of translation in the no-lobed leaf *** prediction showed that Cla018360 encodes an HD-Zip protein that has been reported to regulate the development of leaf *** single-base deletion also occurred in the HD-Zip *** inferred that the Cla018360 gene is the candidate gene for regulating the development of lobed/no-lobed leaves in *** expression analysis showed that Cla018360 was highly expressed in young *** analysis showed that Cla018360 had a close genetic relationship with AtHB51,which had been reported to regulate the formation of leaf shape in ***,transcriptome analysis showed that a total of 333 differentially expressed genes were identified between WT2 and WT20,of which 115 and 218 genes were upregulated and downregulated in no-lobed leaved watermelon *** study not only provides a good entry point for studying leaf development but also provides foundational insights into breeding for special plant a

关键词： Watermelon lobed/no-lobed leaf mapping RNA-seq

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Electrocardiogram Feature Extraction and Pattern Recognition Using a Novel Windowing Algorithm

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Advances in Bioscience and Biotechnology 2014年第11期5卷 886-894页

作者： muhammad umer Bilal Ahmed Bhatti muhammad Hammad Tariq muhammad Zia-ul-Hassan muhammad Yaqub Khan Tahir Zaidi Center for Advanced Research in Engineering (CARE) Islamabad Pakistan

This paper presents a Novel Windowing Algorithm for Electrocardiogram Feature Extraction and Pattern Recognition. The work presented here deals with a simple and efficient way of detecting ECG features that are P, Q, R, S and T waves. Windowing method is used to select these waves. Windows are based on varying R-R intervals. It has been tested on ECG simulator data and also on different records of the MIT-BIH arrhythmia database, producing satisfactory results. ECG timing intervals are also required for monitoring the cardiac condition of patients. Hence after feature detections ECG timing intervals like the PR interval, QRS duration, the QT interval, the QT corrected interval and Vent Rate are efficiently calculated using proposed Formulae.

关键词： Electrocardiogram PLI Windowing Arrhythmia Intervals Cardiac PQRST

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天然草原综合风险评估与区划研究——基于多重风险相互作用的视角

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中国农业资源与区划 2023年第7期44卷 139-149页

作者：郭新雅赵元凤弓宇飞 muhammad umer Arshad 内蒙古农业大学经济管理学院内蒙古大学鸿德文理学院

[目的]在建立健全草原风险管理体系，保障草原生态安全和稳定草原畜牧业生产的背景下，草原保险的作用更加凸显。草原风险评估和区划作为草原保险设计的前提和基础，其准确性直接决定着草原保险条款的合理性及科学性。[方法]文章利用半... 详细信息

[目的]在建立健全草原风险管理体系，保障草原生态安全和稳定草原畜牧业生产的背景下，草原保险的作用更加凸显。草原风险评估和区划作为草原保险设计的前提和基础，其准确性直接决定着草原保险条款的合理性及科学性。[方法]文章利用半定量化因果关系矩阵法、HP滤波模型、多种聚类分析法和单因素方差分析法。[结果]可将锡林郭勒草原分为低、中、高和极高4个风险区，各风险区间的差异显著，其中，低风险区呈现出致灾因子危险性低、承灾体脆弱性低和防灾减灾能力强的显著特点；中风险区呈现出承灾体暴露性水平高，但致灾因子危险性处于中等水平的显著特点；高风险区呈现出致灾因子危险性高，防灾减灾能力处于中等水平的典型特征；极高风险区呈现出承灾体脆弱性水平高，防灾减灾能力弱的典型特征。[结论]在厘定草原保险费率时，应考虑多种风险间的交互作用，构建合理的草原风险区划图。

关键词：天然草原草原风险风险评估风险区划锡林郭勒草原

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The morphological diversity of pollen in the genus Gossypium

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Journal of Cotton Research 2023年第1期6卷 48-59页

作者： CAI Xiaoyan HOU Yuqing muhammad umer Jawad WANG Heng XU Yanchao ZHENG Jie WANG Yuhong LIU Fang ZHOU Zhongli HUA Jinping WANG Kunbo State Key Laboratory of Cotton Biology Institute of Cotton ResearchChinese Academy of Agricultural SciencesAnyang 455000HenanChina National Nanfan Research Institute(Sanya) Chinese Academy of Agriculture SciencesSanya 572025HainanChina College of Agronomy and Biotechnology China Agricultural UniversityBeijing 100193China

Background Plant pollen has diverse morphological characteristics that can be consistently passed down from generation to *** on pollen morphology is thus immensely important for plant classification and *** the genus Gossypium,however,in-depth research on pollen morphology is lacking,with only few reports on limited cotton *** evaluate the diversity of pollen in Gossypium,we therefore conducted a comprehensive analysis of the pollen morphology of 33 cotton species and varieties using scanning electron *** The 33 analyzed cotton samples exhibited common pollen morphological features,including spherical shapes,radial symmetry,echination,panporation,and operculation,while the pollen size,spine shape,spine density and length showed distinctive *** size varied significantly among species,with diameters ranging from62.43 μm in *** to 103.41 μm in *** exine had an echinate sculptural texture,and spines were mostly conical or sharply conical but occasionally *** density varied from 173 in *** to 54 in ***,while spine length ranged from 3.53 μm in *** to 9.47 μm in *** addition,the 33cotton species and varieties were grouped at a genetic distance of 3.83 into three *** Ⅰ comprised five allotetraploid AD-genome cotton species,four D-genome species,and one K-genome *** Ⅱ included 13diploid species from A,B,D,E,and G genomes,whereas Cluster Ⅲ only consisted one E-genome species *** Although pollen characteristics alone are not enough to resolve taxonomic and systematic relationships within the genus Gossypium,our results add to knowledge on palynomorphology and contribute to phenological information on these *** findings should aid future systematic and phylogenetic studies of the Gossypium genus.

关键词： Cotton Scanning electron microscopy Pollen morphology Diversity

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Boosting Achene Yield and Yield Related Traits of Sunflower Hybrids through Boron Application Strategies

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American Journal of Plant Sciences 2015年第11期6卷 1752-1759页

作者： Imran Khan Shakeel Ahmad Anjum Rashad Waseem Khan Qardri Muqarrab Ali muhammad umer Chattha muhammad Asif Department of Agronomy University of Agriculture Faisalabad Pakistan Institute of Horticultural Sciences University of Agriculture Faisalabad Pakistan Department of Agronomy Muhammad Nawaz Shareef University of Agriculture Multan Pakistan

Effects of different boron (B) application methods on growth and yield of sunflower hybrids were checked out at Agronomic Research Farm, University of Agriculture, Faisalabad, Pakistan during spring, 2013. Field experiment was conducted by considering three sunflower hybrids viz., Patron 551, Patron 851 and S-278 along with different methods of B application i.e. no B application, seed treatment @ 0.05% B, soil B application @ 2 kg·ha-1 at sowing, soil B application @ 2 kg·ha-1 at ray floret stage and foliar application of B @ 200 mg·L-1 at ray floret stage. Uttermost plant height (150.78 cm), the number of leaves per plant (22.67) and stem diameter (1.62 cm) were accomplished when boron was soil applied @ 2 kg·ha-1 at sowing. Significantly higher head diameter (18.30 cm), number of achene per head (1266.44), 1000-achene weight (43.17 g) achene yield (2039.33 kg·ha-1), biological yield (9223.11 kg·ha-1) and harvest index (22.10%) were registered when boron was foliar applied @ 200 mg·L-1 at ray floret stage. Among sunflower hybrids, Patron 551 produced significantly higher growth and yield attributes as compared with Patron 851 and S-278 hybrids. This study suggested that the selection of Patron 551 hybrid with practicing boron foliar application @ 200 mg·L-1 at ray floret stage could be helpful in achieving the sunflower crop genetic potential.

关键词： Boron Application Methods Sunflower Hybrids Biological Yield Achene Yield Growth and Yield Attributes

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An attending physician float shift for the improvement of physician productivity in a crowded emergency department

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World Journal of Emergency Medicine 2013年第1期4卷 10-14页

作者： muhammad umer Nasim Chintan Mistry Robert Harwood Erik Kulstad Laura Tommaso Department of Emergency Medicine Advocate Christ Medical CenterOak LawnILUSA Advocate Lutheran General Hospital Park RidgeIllinois 60068USA

BACKGROUND:Patients backlogged in the emergency department(ED) waiting for an inpatient bed(boarders) continue to require the attention of ED physicians,exacerbating crowding in the *** address this problem,we added a "float shift" to our winter schedule solely to care for *** sought to quantify the effect of this float shift,hypothesizing greater physician ***:We performed a retrospective observational study in our community hospital ED,measuring the number of new patients seen in each 10-hour shift in the presence or absence of a float shift *** calculated the number of new patients seen per shift for each of the 7 daily shifts,during February(float shift scheduled) and May(float shift unscheduled) of *** then compared the mean number of patients seen per shift in February with ***:Total monthly patient volume was 6 656 for February and 6 775 for May,with the mean daily census being 230 and 219 patients,*** number of new patients seen during each shift was greater in February than in May,with a mean increase of 1.1 patients per shift(with the float shift).Surveying participants about intervention effectiveness showed 92%of residents,but only 65%of attending physicians,in favor of maintaining the float ***:The presence of a "float shift" physician caring only for boarding patients allows other physicians to maintain and even increase their productivity in our ED,despite the presence of longer throughput times and increased time on diversion.

关键词： Crowding Physician staffing Boarding patients Float shift

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Risk factor profiles for gastric cancer prediction with respect to Helicobacter pylori:A study of a tertiary care hospital in Pakistan

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Artificial Intelligence in Gastroenterology 2023年第1期4卷 10-27页

作者： Shahid Aziz Simone König muhammad umer Tayyab Saeed Akhter Shafqat Iqbal Maryum Ibrar Tofeeq Ur-Rehman Tanvir Ahmad Alfizah Hanafiah Rabaab Zahra Faisal Rasheed Patients Diagnostic Lab Isotope Application DivisionPakistan Institute of Nuclear Science and TechnologyIslamabad 44000Pakistan Department of Microbiology Quaid-i-Azam UniversityIslamabad 45320Pakistan Interdisciplinary Center for Clinical Research Core Unit ProteomicsUniversity of MünsterMünster 48149Germany Management Information System Division Pakistan Institute of Nuclear Science and TechnologyIslamabad 44000Pakistan Centre for Liver and Digestive Diseases Holy Family HospitalRawalpindi 46300Pakistan Pakistan Scientific and Technological Information Centre Quaid-i-Azam UniversityIslamabad 45320Pakistan Department of Pharmacy Quaid-i-Azam UniversityIslamabad 45320Pakistan Faculty of Medicine Department of Medical Microbiology and ImmunologyUniversiti Kebangsan MalaysiaCherasKuala Lumpur 56000Malaysia

BACKGROUND Gastric cancer(GC)is the fourth leading cause of cancer-related deaths *** relies on histopathology and the number of endoscopies is *** pylori(***)infection is a major risk *** To develop an in-silico GC prediction model to reduce the number of diagnostic surgical *** meta-data of patients with gastroduodenal symptoms,risk factors associated with GC,and *** infection status from Holy Family Hospital Rawalpindi,Pakistan,were used with machine *** A cohort of 341 patients was divided into three groups[normal gastric mucosa(NGM),gastroduodenal diseases(GDD),and GC].Information associated with socioeconomic and demographic conditions and GC risk factors was collected using a *** infection status was determined based on urea breath *** association of these factors and histopathological grades was assessed statistically.K-Nearest Neighbors and Random Forest(RF)machine learning models were *** This study reported an overall frequency of 64.2%(219/341)of *** infection among enrolled *** was higher in GC(74.2%,23/31)as compared to NGM and GDD and higher in males(54.3%,119/219)as compared to *** abdominal pain(72.4%,247/341)was observed than other clinical symptoms including vomiting,bloating,acid reflux and *** majority of the GC patients experienced symptoms of vomiting(91%,20/22)with abdominal pain(100%,22/22).The multinomial logistic regression model was statistically significant and correctly classified 80%of the GDD/GC ***,income level,vomiting,bloating and medication had significant association with GDD and GC.A dynamic RF GC-predictive model was developed,which achieved>80%test *** GC risk factors were incorporated into a computer model to predict the likelihood of developing GC with high sensitivity and *** model is dynamic and will be further improved and validated by including n

关键词： Gastric cancer Gastritis Machine learning Prediction model Helicobacter pylori

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Widespread incomplete lineage sorting and introgression shaped adaptive radiation in the Gossypium genus

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Plant Communications 2024年第2期5卷 185-197页

作者： Yanchao Xu Yangyang Wei Zhongli Zhou Xiaoyan Cai Scott ABoden muhammad Jawad umer Luqman BSafdar Yuling Liu Dingsha Jin Yuqing Hou Yuhong Wang Sarah Brooke Wall Kunbo Wang Shuxun Yu Baohong Zhang Renhai Peng Fang Liu National Key Laboratory of Cotton Bio-breeding and Integrated Utilization Institute of Cotton ResearchChinese Academy of Agricultural SciencesAnyang 455000China College of Plant Science and Technology Huazhong Agricultural UniversityWuhan 40070China College of Biology and Food Engineering Anyang Institute of TechnologyAnyang 455000China National Nanfan Research Institute(Sanya) Chinese Academy of Agricultural SciencesSanya 572024China School of Agriculture Food and WineUniversity of AdelaideAdelaideSA 5005Australia Sanya Institute Hainan Academy of Agricultural SciencesSanya 572000China Department of Biology East Carolina UniversityGreenvilleNC 27858USA School of Agricultural Sciences Zhengzhou UniversityZhengzhou 450001China

Cotton(Gossypium)stands as a crucial economic crop,serving as the primary source of naturalfiber for the textile ***,the evolutionary mechanisms driving speciation within the Gossypium genus remain *** this investigation,we leveraged 25 Gossypium genomes and introduced four novel assem-blies—***,***,***,and ***(Gklo)—to delve into the speciation history of this ***,we encountered intricate phylogenies potentially stemming from *** complexities are further compounded by incomplete lineage sorting(ILS),a factor likely to have been instrumental in shaping the swift diversification of *** focus subsequently shifted to the rapid radiation episode during a concise period in Gossypium *** a recently diverged lineage comprising ***,Gklo,and ***,we constructed afinely detailed ILS ***,this analysis revealed the non-random distribution of ILS regions across the reference Gklo ***,we identified signs of robust natural selection influencing specific ILS *** variations per-taining to speciation emerged between the closely related sister species Gklo and ***-mately 15.74%of speciation structural variation genes and 12.04%of speciation-associated genes were esti-mated to intersect with ILS *** enrich our understanding of the role of ILS in adaptive radiation,shedding fresh light on the intricate speciation history of the Gossypium genus.

关键词： cotton speciation Gossypium genus incomplete lineage sorting ILS phylogenetic analysis gene tree resolution

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Shade and microbes enhance drought stress tolerance in plants by inducing phytohormones at molecular levels: a review

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Journal of Plant Ecology 2022年第6期15卷 1107-1117页

作者： muhammad Ahsan Asghar Bushra Ahmad Ali Raza Bilal Adil Hafz Hassan Javed muhammad umer Farooq Abuzar Ghafoor muhammad Iftikhar Hussain Iram Shafq Hassan Karim Xin Sun Wenyu Yang Gábor Kocsy Junbo Du Department of Biological Resources Agricultural InstituteCentre for Agricultural ResearchELKHMartonvásárHungary College of Agronomy Sichuan Agricultural UniversityChengdu 611130China Department of Plant Breeding and Genetics University of AgricultureFaisalabad 38000PunjabPakistan College of Resource Sichuan Agricultural UniversityChengdu 611130China Department of Field Crops Faculty of AgricultureÇukurova UniversityAdana 01330Turkey Department of Plant Biology&Soil Science Faculty of BiologyUniversity of VigoCampus As Lagoas MarcosendeVigo 36310Spain State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural UniversityChengduSichuan 611130China

Plants are frequently exposed to adverse environments during their life *** them drought stress is one of the major threats to agricultural *** order to survive in such unstable environment,plants have developed mechanisms through which they recognize the severity of the stress based on the incoming environmental *** combat the detrimental effects of drought,the plants have evolved various strategies to modulate their physio-hormonal *** strategies that can be modulated by shade and microbes contribute to enhancing tolerance to drought and reducing yield *** hormones,such as abscisic acid,auxin and ethylene have a major role in the shade-and microbe-associated improvement of drought tolerance through their effects on various metabolic *** this process,the CLAVATA3/EMBRYOSURROUNDING REGION-RELATED 25 peptide has a major role due to its effect on ABA synthesis as shown in our regulatory model.

关键词： abscisic acid CLAVATA3/EMBRYOSURROUNDING REGION-RELATED 25 auxin plant growth promoting rhizobacteria ethylene

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Genome wide identification and characterization of light-harvesting Chloro a/b binding(LHC)genes reveals their potential role in enhancing drought tolerance in Gossypium hirsutum

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Journal of Cotton Research 2021年第2期4卷 147-159页

作者： MEHARI Teame Gereziher XU Yanchao MAGWANGA Richard Odongo umer muhammad Jawad KIRUNGU Joy Nyangasi CAI Xiaoyan HOU Yuqing WANG Yuhong YU Shuxun WANG Kunbo ZHOU Zhongli LIU Fang State Key Laboratory of Cotton Biology/Institute of Cotton Research Chinese Academy of Agricultural Sciences(ICRCAAS)Anyang455000HenanChina Ethiopian Institute of Agricultural Research Mekhoni Agricultural Research CenterP.O.Box 47MekhoniTigrayEthiopia School of Biological and Physical Sciences(SBPS) Main CampusJaramogi Oginga Odinga University of Science and Technology(JOOUST)Main CampusP.O.Box 210-40601BondoKenya School of Agricultural Sciences Zhengzhou UniversityZhengzhouHenan450001China

Background: Cotton is an important commercial crop for being a valuable source of natural *** production has undergone a sharp decline because of abiotic stresses,*** is one of the major abiotic stress causing significant yield losses in ***,plants have evolved self-defense mechanisms to cope abiotic factors like drought,salt,cold,*** evolution of stress responsive transcription factors such as the trihelix,a nodule-inception-like protein(NLP),and the late embryogenesis abundant proteins have shown positive response in the resistance improvement to several abiotic ***: Genome wide identification and characterization of the effects of Light-Harvesting Chloro a/b binding(LHC)genes were carried out in cotton under drought stress conditions.A hundred and nine proteins encoded by the LHC genes were found in the cotton genome,with 55,27,and 27 genes found to be distributed in Gossypium hirsutum,***,and ***,*** proteins encoded by the genes were unevenly distributed on various *** Ka/Ks(Non-synonymous substitution rate/Synonymous substitution rate)values were less than one,an indication of negative selection of the gene *** expressions of genes showed that majority of the genes are being highly upregulated in the roots as compared with leaves and stem *** genes were found to be highly expressed in MR-85,a relative drought tolerant ***: The results provide proofs of the possible role of the LHC genes in improving drought stress tolerance,and can be explored by cotton breeders in releasing a more drought tolerant cotton varieties.

关键词： Cotton G hirsutum LHC genes Gene expression Drought tolerance

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