Objective: The detailed knowledge about protective effects of capsaicin(cap) and involved mechanisms against testicular torsion(TT) is still not available ***: Male Wistar rats were assigned into four major cohorts:(i...
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Objective: The detailed knowledge about protective effects of capsaicin(cap) and involved mechanisms against testicular torsion(TT) is still not available ***: Male Wistar rats were assigned into four major cohorts:(i) sham,(ii) TT,(iii) three subgroups subjected to TT and different doses of cap(100, 500, and 1000 μg/mL), and(iv) three subgroups of healthy animals subjected to various concentrations of cap. The animals were decapitated at 24 h after reperfusion, and the evaluation of protein expression was performed by Western blotting assay. At 72 h after reperfusion, apoptotic cell death and tissue injury were evaluated by TUNEL nuclear and H&E staining,***: The results showed that cap administration following TT significantly increased the expression of tuberous sclerosis proteins 1 and 2(Tsc1/Tsc2) in a dose-dependent manner(P < 0.05). Cap decreased cell apoptosis at highest dose. Likewise, cap contributed to the preservation of tubular morphology and decreased tissue injury at the highest tested concentration(1000 μg/m L).Conclusion: Collectively, our findings demonstrate the validity of cap as a therapeutic agent against TT through targeting Tsc1/Tsc2 in a dose-dependent manner.
RAZA Irum1, HU Daowu1, AHMAD Adeel1, LI Hongge1, HE Shoupu1, NAZIR Mian Faisal1, WANG Xiaoyang1,JIA Yinhua1, PAN Zhaoe1, ZHANG Peng1, YASIR muhammad1, IQBAL muhammadshahid1, GENG Xiaoli1,WANG Liru1, PANG Baoyin1and D...
RAZA Irum1, HU Daowu1, AHMAD Adeel1, LI Hongge1, HE Shoupu1, NAZIR Mian Faisal1, WANG Xiaoyang1,JIA Yinhua1, PAN Zhaoe1, ZHANG Peng1, YASIR muhammad1, IQBAL muhammad shahid1, GENG Xiaoli1,WANG Liru1, PANG Baoyin1and DU Xiongming1,2*
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