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Multiscale engineering of microbial cell factories: A step forward towards sustainable natural products industry

Synthetic and Systems Biotechnology 2022年第1期7卷 586-601页

作者： muhammad Hammad Hussain muhammad Zubair mohsin Waqas Qamar Zaman Junxiong Yu Xueli Zhao Yanlong Wei Yingping Zhuang Ali mohsin Meijin Guo State Key Laboratory of Bioreactor Engineering East China University of Science and TechnologyShanghai200237PR China Institute of Environmental Sciences and Engineering School of Civil and Environmental EngineeringNational University of Sciences and Technology(NUST)Sector H-12Islamabad44000Pakistan

Microbial cell factories(bacteria and fungi)are the leading producers of beneficial natural products such as lycopene,carotene,herbal medicine,and biodiesel *** microorganisms are considered efficient due to their effective bioprocessing strategy(monoculture-and consortial-based approach)under distinct processing ***,the advancement in genetic and process optimization techniques leads to enhanced biosynthesis of natural products that are known functional ingredients with numerous applications in the food,cosmetic and medical *** consortia and monoculture thrive in nature in a small proportion,such as wastewater,food products,and *** similitude to natural consortia,it is possible to engineer artificial microbial consortia and program their behaviours via synthetic biology ***,this review summarizes the optimization of genetic and physicochemical parameters of the microbial system for improved production of natural ***,this review presents a brief history of natural consortium and describes the functional properties of *** review focuses on synthetic biology tools that enable new approaches to design synthetic consortia;and highlights the syntropic interactions that determine the performance and stability of synthetic *** particular,the effect of processing conditions and advanced genetic techniques to improve the productibility of both monoculture and consortial based systems have been greatly *** this context,possible strategies are also discussed to give an insight into microbial engineering for improved pro-duction of natural products in the *** summary,it is concluded that the coupling of genomic modifications with optimum physicochemical factors would be promising for producing a robust microbial cell factory that shall contribute to the increased production of natural products.

关键词： Genetic and process optimization techniques Monoculture Natural products Synthetic consortia Synthetic biology tools

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Advances in engineered Bacillus subtilis biofilms and spores, and their applications in bioremediation, biocatalysis, and biomaterials

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Synthetic and Systems Biotechnology 2021年第3期6卷 180-191页

作者： muhammad Zubair mohsin Rabia Omer Jiaofang Huang Ali mohsin Meijin Guo Jiangchao Qian Yingping Zhuang State Key Laboratory of Bioreactor Engineering East China University of Science and TechnologyShanghai 200237PR China

Bacillus subtilis is a commonly used commercial specie with broad applications in the fields of bioengineering and *** is capable of producing both biofilms and *** are matrix-encased multicellular communities that comprise various components including exopolysaccharides,proteins,extracellular DNA,and poly-γ-glutamic *** biofilms resist environmental conditions such as oxidative stress and hence have applications in bioremediation ***,biofilms and spores can be engineered through biotechnological techniques for environmentally-friendly and safe production of bio-products such as *** ability to withstand with harsh conditions and producing spores makes Bacillus a suitable candidate for surface display *** recent years,the spores of such specie are widely used as it is generally regarded as safe to *** in synthetic biology have enabled the reprogramming of biofilms to improve their functions and enhance the production of value-added ***,there is increased interest in the production of engineered biosensors,biocatalysts,and *** elastic modulus and gel properties of *** biofilms have been utilized to develop living *** review outlines the formation of *** biofilms and *** engineering processes and their increasing application in bioremediation and biocatalysis,as well as the future directions of *** biofilm engineering,are ***,the ability of *** biofilms and spores to fabricate functional living materials with self-regenerating,self-regulating and environmentally responsive characteristics has been *** review aims to resume advances in biological engineering of *** biofilms and spores and their applications.

关键词： Bacillus subtilis Biofilms Spores Biocatalysis Bioremediation Biomaterials Synthetic biology

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Deep Learning-based Environmental Sound Classification Using Feature Fusion and Data Enhancement

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Computers, Materials & Continua 2023年第1期74卷 1069-1091页

作者： Rashid Jahangir muhammad Asif Nauman Roobaea Alroobaea Jasem Almotiri muhammad mohsin Malik Sabah M.Alzahrani Department of Computer Science COMSATS University IslamabadVehari CampusPakistan Department of Computer Science University of Engineering and Technology LahorePakistan Department of Computer Science College of Computers and Information TechnologyTaif UniversityTaif21974Saudi Arabia

Environmental sound classification(ESC)involves the process of distinguishing an audio stream associated with numerous environmental *** common aspects such as the framework difference,overlapping of different sound events,and the presence of various sound sources during recording make the ESC task much more complicated and *** research is to propose a deep learning model to improve the recognition rate of environmental sounds and reduce the model training time under limited computation *** this research,the performance of transformer and convolutional neural networks(CNN)are *** audio features,chromagram,Mel-spectrogram,tonnetz,Mel-Frequency Cepstral Coefficients(MFCCs),delta MFCCs,delta-delta MFCCs and spectral contrast,are extracted fromtheUrbanSound8K,ESC-50,and ESC-10,***,this research also employed three data enhancement methods,namely,white noise,pitch tuning,and time stretch to reduce the risk of overfitting issue due to the limited audio *** evaluation of various experiments demonstrates that the best performance was achieved by the proposed transformer model using seven audio features on enhanced *** UrbanSound8K,ESC-50,and ESC-10,the highest attained accuracies are 0.98,0.94,and 0.97 *** experimental results reveal that the proposed technique can achieve the best performance for ESC problems.

关键词： Environmental sound classification convolutional neural network deep learning transformer data augmentation

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Energy Theft Detection in Smart Grids:Taxonomy,Comparative Analysis,Challenges,and Future Research Directions

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IEEE/CAA Journal of Automatica Sinica 2022年第4期9卷 578-600页

作者： mohsin Ahmed Abid khan Mansoor Ahmed Mouzna Tahir Gwanggil Jeon Giancarlo Fortino Francesco Piccialli the Department of Computer Science COMSATS University Islamabad the Department of Computer Science School of ComputingEngineering and Digital TechnologiesTeesside University the Innovative Value Institute Maynooth University the Department of Computer Science Bahria University the School of Electronic Engineering Xidian University the Department of Embedded Systems Engineering Incheon National University IEEE the Department of Informatics ModelingElectronics and SystemsUniversity of Calabria the Department of Mathematics and Applications“R.Caccioppoli” University of Naples FedericoⅡ

Electricity theft is one of the major issues in developing countries which is affecting their economy *** with the introduction of emerging technologies,this issue became more *** many new energy theft detection(ETD)techniques have been proposed by utilising different data mining(DM)techniques,state&network(S&N)based techniques,and game theory(GT)***,a detailed survey is presented where many state-of-the-art ETD techniques are studied and analysed for their strengths and *** levels of taxonomy are presented to classify state-of-the-art ETD *** types and ways of energy theft and their consequences are studied and summarised and different parameters to benchmark the performance of proposed techniques are extracted from *** challenges of different ETD techniques and their mitigation are suggested for future *** is observed that the literature on ETD lacks knowledge management techniques that can be more effective,not only for ETD but also for theft *** can help in the prevention of energy theft,in the future,as well as for ETD.

关键词： Challenges comparative analysis energy theft detection future research directions smart grid taxonomy

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Advancing Al-doped ZnO thin films structural,optical and electrical properties of low temperature PET substrates via flash lamp annealing

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Journal of Semiconductors 2024年第12期45卷 47-60页

作者： Juwon Lee Chang-Hyeon Jo Gwangseop Lee Min-Sung Bae Slawomir Prucnal Shengqiang Zhou muhammad Zubair khan Osama Gohar mohsin Saleem Jung-Hyuk Koh Graduate School of Intelligent Energy and Industry Chung-Ang UniversityHeukseok-roSeoulRepublic of Korea School of Electrical and Electronic Engineering Chung-Ang UniversitySeoulRepublic of Korea Helmholtz-Zentrum Dresden-Rossendorf Institute of Ion Beam Physics and Materials ResearchBautzner Landstraße 40001328DresdenGermany Department of Materials Science and Engineering Pak-Austria Fachhochschule:Institute of Applied Sciences and TechnologyMangHaripur22621Khyber PakhtunkhwaPakistan Department of Mechanical and Electrical Systems Engineering Graduate School of EngineeringKyoto University of Advanced ScienceGotanda-choYamanouchiUkyo-kuKyoto 615-8577Japan School of Chemical and Materials Engineering(SCME) National University of Sciences&Technology(NUST)-H12Islamabad 44000Pakistan

In this study,aluminum-doped zinc oxide(AZO)thin films were deposited onto a low-temperature polyethylene terephthalate(PET)substrate using DC magnetron *** parameters included power range of 100-300 W,a working pressure of 15 mTorr,and a substrate temperature of 50°***-deposition,flash lamp annealing(FLA)was employed as a rapid thermal processing method with a pulse duration of 1.7 ms and energy density of 7 J·cm-2,aimed at enhancing the film's quality while preserving the temperature-sensitive PET *** offers advantages over conventional annealing,including shorter processing times and improved material *** structural,optical,and electrical characteristics of the AZO films were assessed using X-ray diffraction,field emission scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy,ultraviolet-visible spectroscopy,and Hall effect *** results demonstrated that properties of AZO films varied with deposition and annealing *** deposited at 200 W and subjected to FLA exhibited superior crystallinity,with average visible light transmittance exceeding 80%and resistivity as low as 0.38Ω·cm representing 95%improvement in *** analysis revealed that carrier concentration,mobility,and resistivity were influenced by both sputtering and annealing *** findings underscore the effectiveness of FLA in optimizing AZO thin film properties,highlighting potential in optoelectronics applications.

关键词： flash lamp annealing AZO thin film PET sputtering

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Seed germination and seedling emergence of four tree species of southern China in response to acid rain

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Journal of Forestry Research 2021年第2期32卷 471-481页

作者： Matoor mohsin Gilani Mulualem Tigabu Bo Liu Taimoor Hassan Farooq muhammad Haroon URashid muhammad Ramzan Xiangqing Ma Forestry College Fujian Agriculture and Forestry UniversityFuzhou 350002People’s Republic of China Fujian Provincial Colleges and University Engineering Research Center of Plantation Sustainable Management Fuzhou 350002People’s Republic of China Faculty of Forest Science Southern Swedish Forest Research CenterSwedish University of Agricultural SciencesP.O.Box 4923053 AlnarpSweden National Engineering Laboratory for Applied Technology of Forestry and Ecology in South China Central South University of Forestry and TechnologyChangsha 410000People’s Republic of China School of Soil and Water Conservation and Desertification Combating Beijing Forestry UniversityBeijing 100083People’s Republic of China

Acid rain has become a major concern due to increasing atmospheric pollution,particularly in *** investigated whether acid rain inhibits the germination of seeds and subsequent emergence of seedling of four tree species from southern China:Cunninghamia lanceolata,Fokienia hodginisi,Pinus massoniana and Phoebe zhennan by simulating acid rain with pH of 2.5,3.5,4.5 and *** hypothesized that the inhibitory effect of acid rain on germination of seeds and emergence of seedling varies between species and the degree of acidity.A solution of 1 N H2SO4 and 1 N HNO 3 in the ratio of 10–1 was prepared and diluted to four pH levels,and seeds were supplied with solutions of these pH values and distilled water as control and tested for germination in a controlled growth *** results revealed that simulated acid rain of pH 2.5 adversely affected the germination capacity of *** and ***;while all acid solutions significantly increased germination of ***;but had no effect on germination of *** *** acid solution(pH of 2.5)adversely affected elongation of radicle and hypocotyl as well as fresh and dry weights of radicle and hypocotyl of tender *** result demonstrated that seedling emergence is more sensitive than seed germination to simulated acid rain,and germination of conifer species are less sensitive than broad leaved species to simulated acid *** a whole,acid rain of pH of 3.5 is the threshold level and acid rain below this value will have a detrimental effect on seed germination and seedling emergence.

关键词： Cunninghamia lanceolata Fokienia hodginisi Pinus massoniana Phoebe zhennan Seed viability Simulated acid rain

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Inducing opto-electronic and spintronic trends in bilayer h-BN through TMO3 clusters incorporation: Ab-initio study

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Chinese Physics B 2019年第11期28卷 284-294页

作者： Irfan Ahmed muhammad Rafique Mukhtiar Ahmed Mahar Abdul Sattar Larik mohsin Ali Tunio Yong Shuai Mehran University of Engineering and Technology SZABCampusKhairpur Mirs’Pakistan School of Energy Science and Engineering Harbin Institute of TechnologyHarbin 150001China Mehran University of Engineering and Technology JamshoroSindhPakistan

The band structure, magnetism, charge distribution, and optics parameters of TMO3–h-BN hybrid systems are investigated by adopting first-principles study(FPS) calculations. It is observed that the TMO3 clusters add finite magnetic moments to bilayer h-BN(BL/h-BN), thereby making it a magnetic two-dimensional(2D) material. Spin-polarized band structures for various TMO3–BL/h-BN hybrid models are calculated. After the incorporation of TMO3, BL/h-BN is converted into semimetal or conducting material in spin up/down bands, depending on the type of impurity cluster present in BL/h-BN lattice. Optics parameters are also investigated for the TMO3–BL/h-BN complex systems. The incorporation of TMO3 clusters modifies the absorption and extinction coefficient in visible range, while static reflectivity and refraction parameter increase. It can be surmised that the TMO3 substitution in BL/h-BN is a suitable technique to modify its physical parameters thus making it functional for nano/opto-electronic applications, and an experimental approach can be adapted to reinforce the outcomes of this study.

关键词： bilayer h-BN band structure density of states magnetic moments optics

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A study of nonlinear biochemical reaction model

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International Journal of Biomathematics 2016年第5期9卷 121-129页

作者： muhammad Asad Iqbal Syed Tauseef Mohyud-Din Bandar Bin-mohsin Department of Mathematics HITEC University Taxila Pakistan Department of Mathematics College of Science King Saud University Riyadh Saudi Arabia

The present study deals with the introduction of an alteration in Legendre wavelets method by availing of the Picard iteration method for system of differential equations and named it Legendre wavelet-Picard method （LWPM）. Convergence of the proposed method is also discussed. In order to check the competence of the proposed method, basic enzyme kinetics is considered. Systems of nonlinear ordinary differential equations are formed from the considered enzyme-substrate reaction. The results obtained by the proposed LWPM are compared with the numerical results obtained from Runge-Kutta method of order four （RK-4）. Numerical results and those obtained by LWPM are in excellent conformance, which would be explained by the help of table and figures. The proposed method is easy and simple to implement as compared to the other existing analytical methods used for solving systems of differential equations arising in biology, physics and engineering.

关键词： Legendre wavelets method Picard iteration method nonlinear biochemical reaction model Runge- Kutta method of order four.

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Artificial Intelligence and Internet of Things Enabled Intelligent Framework for Active and Healthy Living

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Computers, Materials & Continua 2023年第5期75卷 3833-3848页

作者： Saeed Ali Alsareii mohsin Raza Abdulrahman Manaa Alamri Mansour Yousef AlAsmari muhammad Irfan Hasan Raza muhammad Awais Department of Surgery College of MedicineNajran University Saudi ArabiaNajran61441Saudi Arabia Department of Computer Science Edge Hill UniversitySt Helens RdOrmskirkL394QPUnited Kingdom Electrical Engineering Department College of EngineeringNajran University Saudi ArabiaNajran61441Saudi Arabia Department of Electrical Engineering Hamdard UniversityIslamabadPakistan

Obesity poses several challenges to healthcare and the well-being of *** can be linked to several life-threatening *** is a viable option in some instances to reduce obesity-related risks and enable weight ***-of-the-art technologies have the potential for long-term benefits in post-surgery *** this work,an Internet of Things(IoT)framework is proposed to effectively communicate the daily living data and exercise routine of surgery patients and patients with excessive *** proposed IoT framework aims to enable seamless communications from wearable sensors and body networks to the cloud to create an accurate profile of the *** also attempts to automate the data analysis and represent the facts about a *** IoT framework proposes a co-channel interference avoidance mechanism and the ability to communicate higher activity data with minimal impact on the bandwidth requirements of the *** proposed IoT framework also benefits from machine learning based activity classification systems,with relatively high accuracy,which allow the communicated data to be translated into meaningful information.

关键词： Artificial intelligence healthcare obesity Internet of Things machine learning physical activity classification activity monitoring

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Risk of infection with arboviruses in a healthy population in Pakistan based on seroprevalence

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Virologica Sinica 2024年第3期39卷 369-377页

作者： Shengyao Chen muhammad Saqib Hafiz Sajid khan Yuan Bai Usman Ali Ashfaq muhammad Khalid Mansoor Abulimti Moming Jing Liu Min Zhou Saifullah khan Niazi Qiaoli Wu Awais-Ur-Rahman Sial Shuang Tang muhammad Hassan Sarfraz Aneela Javed Sumreen Hayat mohsin Khurshid Iahtasham khan muhammad Ammar Athar Zeeshan Taj Bo Zhang Fei Deng Ali Zohaib Shu Shen Key Laboratory of Virology and National Virus Resource Center Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China University of Chinese Academy of Sciences Beijing 100049China Faculty of Veterinary Science University of Agriculture FaisalabadFaisalabad 38040Pakistan The Children's Hospital Faisalabad 38000Pakistan Department of Bioinformatics and Biotechnology Government College University FaisalabadFaisalabad 38000Pakistan f Department of Microbiology Faculty of Veterinary and Animal SciencesThe Islamia University of BahawalpurBahawalpur 63100Pakistan Hubei Jiangxia Laboratory Wuhan 430200China Key Laboratory of Special Pathogens and Biosafety Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhan 430071China Armed Forces Institute of Pathology(AFIP) Rawalpindi 46000Pakistan Department of Clinical Studies Faculty of Veterinary&Animal SciencesPir Mehr Ali Shah Arid Agriculture UniversityRawalpindi 46300Pakistan Institute of Microbiology Government College University FaisalabadFaisalabad 38000Pakistan l Atta-ur-Rahman School of Applied Biosciences National University of Science and TechnologyIslamabad 44000Pakistan Department of Clinical Sciences University of Veterinary and Animal SciencesLahore Sub-campusJhang 35200Pakistan Department of Molecular Pathology National Medical Center DHA Phase 1Karachi 75500Pakistan

Infectious diseases caused by arboviruses are a public health concern in ***,studies on data prevalence and threats posed by arboviruses are *** study investigated the seroprevalence of arboviruses in a healthy population in Pakistan,including severe fever with thrombocytopenia syndrome virus(SFTSV),Crimean-Congo hemorrhagic fever virus(CCHFV),Tamdy virus(TAMV),and Karshi virus(KSIV)based on a newly established luciferase immunoprecipitation system(LIPS)assays,and Zika virus(ZIKV)by enzyme-linked immunosorbent assays(ELISA).Neutralizing activities against these arboviruses were further examined from the antibody positive *** results showed that the seroprevalence of SFTSV,CCHFV,TAMV,KSIV,and ZIKV was 17.37%,7.58%,4.41%,1.10%,and 6.48%,respectively,and neutralizing to SFTSV(1.79%),CCHFV(2.62%),and ZIKV(0.69%)were identified,as well as to the SFTSV-related Guertu virus(GTV,0.83%).Risk factors associated with the incidence of exposure and levels of antibody response were ***,co-exposure to different arboviruses was demonstrated,as thirty-seven individuals were having antibodies against multiple viruses and thirteen showed neutralizing ***,individuals aged40 years,and outdoor workers had a high risk of exposure to *** these results reveal the substantial risks of infection with arboviruses in Pakistan,and indicate the threat from co-exposure to multiple *** findings raise the need for further epidemiologic investigation in expanded regions and populations and the necessity to improve health surveillance in Pakistan.

关键词： Arbovirus Pakistan Seroprevalence Tick-borne virus Mosquito-borne virus

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