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Study of coronary sinus anatomy during levophase of coronary angiography

World Journal of Cardiology 2022年第6期14卷 372-381页

作者： Akshyaya Pradhan Vrishank Bajaj Pravesh Vishwakarma Monika Bhandari Akhil Sharma Gaurav Chaudhary Sharad Chandra Rishi sethi Varun Shankar Narain Sudhanshu Dwivedi Department of Cardiology King George Medical UniversityLucknow 226003Uttar PradeshIndia

BACKGROUND Coronary sinus(CS)imaging has recently gained importance due to increasing need for mapping and ablation of electrophysiological arrhythmias and left ventricular(LV)pacing during cardiac resynchronization therapy(CRT).Retrograde venogram is the current standard for imaging CS and its *** To evaluate CS anatomy during levophase of routine coronary angiography to aid LV lead implantation during *** In this prospective observational study,164 patients undergoing routine coronary angiography for various indications(Chronic stable angina-44.5%,acute coronary syndrome-39.5%,Dilated cardiomyopathy-11%,atypical chest pain-5%)were *** phase(levophase)of left coronary injection was recorded in left anterior oblique-cranial and right anterior oblique-cranial *** of coronary veins,width and shape of CS ostium,angulations of proximal CS with body of CS were ***,size,take-off angle and tortuosity of posterolateral vein(PLV),anterior interventricular veins(AIV)and middle cardiac vein(MCV)were also *** During levophase,visibility grade(Muhlenbruch grade)for coronary veins was 3 in 74%and 2 in 26%of *** of CS did not correlate with body mass *** diameter of CS ostium was15 mm in 48%,42%and 10%of patients *** CS was tubular in 136(83%)patients and funnel-shaped in 28(17%)*** take-off angulation between ostium and body of CS was seen in 16(10%)*** or more PLV were present in 8 patients while PLV was absent in 52(32%)*** of take-off of PLV with body of CS was favourable(0°-45°)in 65(40%)*** angle was 45°-90°in 36 patients and difficult take-off angle(>90°)was seen in 8 *** of PLV reached distal third of myocardium in 84 cases and middle third in 11 *** was no tortuosity in 79 cases,a single bend in 29 cases and more than 2 bends in 4 *** nine(24%)patients had other veins supplying poste

关键词： Posterolateral vein Ostium Angiography Sharp take off Favourable angulation

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An Improved High Performance Liquid Chromatographic Method for Identification and Quantization of Polyamines as Benzoylated Derivatives

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American Journal of Analytical Chemistry 2011年第4期2卷 456-469页

作者： Rajat sethi Sai Raghuveer Chava Sajid Bashir Mauro E. Castro 不详

A simple reversed phase HPLC method was developed for the determination of Putrescine, Cadaverine and Spermidine (a class of polyamines) in their benzoylated form from external known standards. In the optimization procedure, a number of parameters were examined: 1) Solvent used in the extraction of standard polyamines (diethyl ether versus chloroform);2) Solvent used in the elution of the polyamine (methanol versus acetonitrile);3) Mode of derivatization and extraction step(s) (derivatization and extraction performed together versus derivatization and extraction performed separately);and 4) Other instrumental parameters (such as UV detection wavelength, gradient profiles). The advantages of our method, relative to the standard Morgan method are: a) decreased chromatographic runtime, b) ease of preparation with good resolution, sensitivity, and reproducibility using a standard RP-HPLC method.

关键词： Polyamine RP-HPLC Standardization Benzoylation

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Injuries in survivors of elephant attack:Report of three cases

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Chinese Journal of Traumatology 2021年第6期24卷 394-396页

作者： Pradeep Kumar Singh S Manwar Ali Mahesh sethi Das Birendra Manohar Department of General Surgery AIIMSBhubaneswarOdishaIndia

Human-elephant conflict(HEC)in India is becoming a growing health problem causing many fatalities every *** produce injuries by trampling,stomping,squeezing,tossing in the air,or crushing/targeting the head and chest *** adult elephants are most aggressive in their mating season,leading to maximum incidences of HECs in this *** attacks are mostly unprovoked,though most HECs are *** this case series,the authors described the injuries sustained by three survivors in a short span of one month due to the sudden and unprovoked elephant *** the injuries were mild to moderate in severity and involved the chest in *** rescue and prompt initiation of treatment were pivotal in their *** authors also want to create awareness about the mating season of elephants to minimize these unfortunate events in the future.

关键词： Elephants Elephant attack Human-elephant conflict Musth

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Role of Polyamines in Ozone Exposed Ischemic-Reperfused Hearts

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American Journal of Analytical Chemistry 2011年第7期2卷 832-839页

作者： Rajat sethi Sai Raghuveer Chava Sajid Bashir Mauro E. Castro 不详

The effect of chronic ozone exposure to ischemia reperfusion (I/R) injury in isolated perfused rat hearts was previously demonstrated. The present study tested our hypothesis that chronic ozone exposure led to attenuation of polyamines in the heart, which may limit sensitivity to I/R. Sprague Dawley rats were continuously exposed for 8 hrs/day for 28 days to filtered air or 0.8 ppm ozone. Isolated hearts were previously subjected to 0.5 hour of global ischemia followed by 1 hour of reperfusion after which global polyamine content was examined between the two groups. Spermidine production was significantly increased in the experimental group compared to control group (of I/R hearts). These results suggest that ozone-induced sensitivity to chronic I/R injury activates myocardial polyamine stress response characterized by increased enzymatic activities and accumulation of spermidine. Collectively, these results suggest that I/R possibly disturbs polyamine metabolism, and increased oxidative stress and concomitant reduced myocardial cell viability.

关键词： Environmental Pollutants Cardiovascular Disease Polyamines Ischemia-Reperfusion (I/R) Injury Oxidative Stress

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Gut microbiome:the third musketeer in the cancer-immune system cross-talk

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Journal of Pancreatology 2020年第4期3卷 181-187页

作者： Prateek Sharma Tejeshwar Jain Vrishketan sethi Srikanth Iyer Vikas Dudeja DeWitt Daughtry Family Department of Surgery Jackson Memorial HospitalUniversity of MiamiMiamiFLUSA

The fascinating hypothesis that microbes lead to cancer has long been dwelled upon but has only been recently investigated in cancers other than those of stomach and *** can affect cancer cells directly through toxins or *** also play a significant role in the development and maturation of the immune system and can indirectly affect cancer cells through the immune *** mouse models and affordable sequencing technologies,have made it possible in current times,to delineate the role of microbes in undermining cancer immune surveillance and indirectly promoting *** this review,we explore the cancer-gut microbiome-immune trialogue,focusing on pancreatic *** review how gut-microbiome interacts with immune system to promote oncogenesis and how modulation of gut-microbiome can be used as an effective therapeutic strategy to reprogram the immune system for augmenting anti-tumor response and enhance immunotherapy efficacy in traditionally resistant pancreatic cancer.

关键词： Gut microbiome Immunotherapy Pancreatic cancer

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Cryptococcosis in kidney transplant recipients:Current understanding and practices

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World Journal of Nephrology 2023年第5期12卷 120-131页

作者： Priti Meena Vinant Bhargava Kulwant Singh Jasmine sethi Aniketh Prabhakar Sandip panda Department of Nephrology All India Institute of Medical SciencesBhubaneswar 751019OdhishaIndia Department of Nephrology Sir Ganga Ram Hospital New DelhiNew Delhi 110001New DelhiIndia Department of Nephrology Ivy HospitalMohali PunjabMohali 160071PunjabIndia Department of Nephrology Post Graduate Institute of Medical Education&ResearchChandigarh 160012PunjabIndia Department of Nephrology Consultant NephrologistSigma HospitalMysore 570009KarnatakaIndia

Cryptococcosis is the third most commonly occurring invasive fungal disease in solid organ transplant recipients(SOT).It is caused by encapsulated yeast,Cryptococcus species,predominantly Cryptococcus neoformans and Cryptococcus *** kidney transplant recipients are at the lowest risk of cryptococcosis when compared to other solid organ transplant recipients such as lung,liver or heart,still this opportunistic infection causes significant morbidity and mortality in this subset of *** rates with cryptococcosis range from 10%-25%,while it can be as high as 50%in SOT recipients with central nervous system *** main aim of diagnosis is to find out if there is any involvement of the central nervous system in disseminated disease or whether there is only localized pulmonary involvement as it has implications for both prognostication and *** of cryptococcal antigen(CrAg)in cerebrospinal fluid or plasma is a highly recommended test as it is more sensitive and specific than India ink and fungal *** CrAg lateral flow assay is the single point of care test that can rapidly detect cryptococcal polysaccharide *** of cryptococcosis is challenging in kidney transplant *** from the reduction or optimization of immunosuppression,lipid formulations of amphotericin B are preferred as induction antifungal *** and maintenance are done with fluconazole;carefully monitoring its interactions with calcineurin *** review further discusses in depth the evolving developments in the epidemiology,pathogenesis,diagnostic assays,and management approach of cryptococcosis in kidney transplant recipients.

关键词： Cryptococcosis Kidney transplant recipients Amphotericin B Immunosuppression Fluconazole

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Ameta-analysis on efficacy and safety:single-balloon ***-balloon enteroscopy

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Gastroenterology Report 2015年第2期3卷 148-155页

作者： Vaibhav Wadhwa Saurabh sethi Sumeet Tewani Sushil Kumar Garg Douglas K.Pleskow Ram Chuttani Tyler M.Berzin Nidhi sethi Mandeep S.Sawhney Department of Internal Medicine Fairview HospitalCleveland ClinicClevelandOHUSA Division of Gastroenterology Beth Israel Deaconess Medical Center and Harvard Medical SchoolBostonMAUSA Division of Basic and Translational Research Department of SurgeryUniversity of MinnesotaMinneapolisMNUSA

Background and aim:Double-balloon enteroscopy(DBE)and single-balloon enteroscopy(SBE)are new techniques capable of providing deep *** of individual studies comparing these techniques have not been able to identify a superior *** aim was to systematically pool all available studies to compare the efficacy and safety of DBE with SBE for evaluation of the small ***:Databases were searched,including PubMed,Embase,and the Cochrane Central Register of Controlled *** main outcome measures were complete small-bowel visualization,diagnostic yield,therapeutic yield,and complication *** analysis was performed using Review Manager(RevMan version 5.2).Meta-analysis was performed using fixed-effect or random-effect methods,depending on the absence or presence of significant *** used the v2 and I2 test to assess heterogeneity between *** were expressed as risk ratios(RR)or mean differences with 95%confidence intervals(CI).Results:Four prospective,randomized,controlled trials with a total of 375 patients were *** was superior to SBE for visualization of the entire small bowel[pooled RR=0.37(95%CI:0.19–0.73;P=0.004)].DBE and SBE were similar in ability to provide diagnosis[pooled RR=0.95(95%CI:0.77–1.17;P=0.62)].There was no significant difference between DBE and SBE in therapeutic yield[pooled RR=0.78(95%CI:0.59–1.04;P=0.09)]and complication rate[pooled RR=1.08(95%CI:0.28–4.22);P=0.91].Conclusions:DBE was superior to SBE with regard to complete small bowel *** was similar to SBE with regard to diagnostic yield,ability to provide treatment and complication rate,but these results should be interpreted with caution as they is based on very few studies and the overall quality of the evidence was rated as low tomoderate,due to the small sample size.

关键词： small bowel enteroscopy single-balloon enteroscopy double-balloon enteroscopy meta-analysis outcomes

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Utility of flexible fiberoptic bronchoscopy for critically ill pediatric patients:A systematic review

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World Journal of Critical Care Medicine 2015年第1期4卷 77-88页

作者： Aida Field-Ridley Viyeka sethi Shweta Murthi Kiran Nandalike Su-Ting T Li Department of Pediatrics University of California DavisSacramentoCA 95618United States

AIM: To investigate the diagnostic yield, therapeutic efficacy, and rate of adverse events related to flexible fiberoptic bronchoscopy(FFB) in critically ill children. METHODS: We searched Pub Med, SCOPUS, OVID, and EMBASE databases through July 2014 for English language publications studying FFB performed in the intensive care unit in children < 18 years old. We identified 666 studies, of which 89 full-text studies were screened for further review. Two reviewers independently determined that 27 of these studies met inclusion criteria and extracted data. We examined the diagnostic yield of FFB among upper and lower airway evaluations, as well as the utility of bronchoalveolar lavage(BAL). RESULTS: We found that FFB led to a change in medical management in 28.9%(range 21.9%-69.2%) of critically ill children. The diagnostic yield of FFB was 82%(range 45.2%-100%). Infectious organisms were identified in 25.7%(17.6%-75%) of BALs performed, resulting in a change of antimicrobial management in 19.1%(range: 12.2%-75%). FFB successfully reexpanded atelectasis or removed mucus plugs in 60.3%(range: 23.8%-100%) of patients with atelectasis. Adverse events were reported in 12.9%(range: 0.5%-71.4%) of patients. The most common adverse effects of FFB were transient hypotension, hypoxia and/or bradycardia that resolved with minimal intervention, such as oxygen supplementation or removal of the bronchoscope. Serious adverse events were uncommon; 2.1% of adverse events required intervention such as bag-mask ventilation or intubation and atropine for hypoxia and bradycardia, normal saline boluses for hypotension, or lavage and suctioning for hemorrhage. CONCLUSION: FFB is safe and effective for diagnostic and therapeutic use in critically ill pediatric patients.

关键词： Bronchoscopy Critical illness Pediatrics Bronchoalveolar lavage Pulmonary disease

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将黑麦基因导入小麦的途径

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国外农学（麦类作物） 1991年第6期 3-5页

作者： G.S.sethi 徐旗田增荣

在小麦产量、品质和适应性改良方面,黑麦具有许多可供利用的优良性状基因.虽然黑麦与小麦染色体组之间有部分同源关系,但由于它们的染色体难以配对,使得利用黑麦与小麦杂交直接将黑麦基因导入小麦有一定的困难.有些染色体操作方法可将... 详细信息

在小麦产量、品质和适应性改良方面,黑麦具有许多可供利用的优良性状基因.虽然黑麦与小麦染色体组之间有部分同源关系,但由于它们的染色体难以配对,使得利用黑麦与小麦杂交直接将黑麦基因导入小麦有一定的困难.有些染色体操作方法可将黑麦基因导入小麦,例如部分同源染色体配对抑制基因(Ph)的利用.试验证明,六倍体小黑麦与普通小麦杂交是将黑麦基因或染色质片段导入小麦的一种很有潜力的方法.通过这条途径已经把黑麦的抗腥黑穗病、条锈及叶锈病,抗旱、长粒和致密腊质层基因或载有这些基因的小染色质片段导入了小麦,有些带有黑麦性状的品系还表现出高产潜力.

关键词：黑麦基因小麦染色体育种

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一种确保qPCR和RT-qPCR实验成功的方法

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生物产业技术 2011年第2期 36-39页

作者： Manju sethi Thermo Scientific NanoDrop Products

实时PCR方法功能强大，若能正确运用，可以获得极有价值的检测结果。按照MIQE指南规范实验．注重PCR检测前待测样品的制备步骤，都将有助于确保实验的成功。

关键词：实验 PCR方法 PCR检测检测结果制备步骤

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