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New techniques of on-line biological sample processing and their application in the field of biopharmaceutical analysis

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Acta Pharmaceutica Sinica B 2016年第6期6卷 540-551页

作者： Jie Peng Fang Tang Rui Zhou Xiang Xie Sanwang Li Feifan Xie Peng Yu lingli mu School of Pharmaceutical Sciences Central South University Medical College Hunan Normal University

Biological sample pretreatment is an important step in biological sample analysis. Due to the diversity of biological matrices, the analysis of target substances in these samples presents significant challenges to sample processing. To meet these emerging demands on biopharmaceutical analysis, this paper summarizes several new techniques of on-line biological sample processing: solid phase extraction, solid phase micro-extraction, column switching, limited intake filler, molecularly imprinted solid phase extraction, tubular column, and micro-dialysis. We describe new developments, principles, and characteristics of these techniques, and the application of liquid chromatography mass spectrometry (LC MS) in biopharmaceutical analysis with these new techniques in on-line biological sample processing. (C) 2016 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND

关键词： Biological sample pretreatment Solid phase micro-extraction Column rbulent flow hromatography Restricted access material Molecular imprinting solid phase extraction

Intracellular pharmacokinetic study of zidovudine and its phosphorylated metabolites

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Acta Pharmaceutica Sinica B 2016年第2期6卷 158-162页

作者： lingli mu Rui Zhou Fang Tang Xingling Liu Sanwang Li Feifan Xie Xiang Xie Jie Peng Peng Yu Medical College Hunan Normal University School of Pharmaceutical Sciences Central South University

Zidovudine (AZT), the first drug approved by the US Food and Drug Administration for the treatment of human immunodeficiency virus (HIV) infection, is metabolized in the host cells to 5'-AZT triphosphate (AZT-TP) which inhibits HIV reverse transcriptase. As the pharmacokinetics of AZT and its phosphorylated metabolites in human peripheral blood mononuclear cells (hPBMCs) is limited, the aim of this study was to determine the pharmacokinetic parameters of AZT and its phosphorylated metabolites in hPBMCs from 12 healthy Chinese male subjects after a single oral dose of 600 mg of AZT. Blood samples were collected prior to drug administration, then at 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 5, 6, 8 and 10 h after drug administration. Mononuclear cells collected by Ficoll-Hypaque density gradient centrifugation were used for determination of AZT and metabolites [AZT monophosphate (AZT-MP), AZT diphosphate (AZT-DP) and AZT-TP] and the plasma was used to evaluate the pharmacokinetics of AZT. Plasma concentration of AZT peaked within 0.583 h and intracellular concentrations of AZT, AZT-MP, AZT-DP and AZT-TP peaked within 1.083, 1.500, 1.417 and 1.583 h, respectively. AZT in plasma was eliminated rapidly with t(1/2) of 2.022 h, and AZT-MP, AZT-DP and AZT-TP were eliminated with t(1/2) of 13.428, 8.285 and 4.240 h, respectively. The plasma concentration of the phosphorylated metabolites was not quantifiable. (C) 2016 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V.

关键词： LC-MS/MS Zidovudine Metabolites Phosphates hPBMCs hPBMCs Pharmacokinetics Intracellular killed

Implementation of a reference-scaled average bioequivalence approach for highly variable generic drug products of agomelatine in Chinese subjects

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Acta Pharmaceutica Sinica B 2016年第1期6卷 71-78页

作者： Fang Tang Rui Zhou Zeneng Cheng Guoping Yang Aiqiao Chen Zhi Liu Hongyi Tan Shuang Yang Sanwang Li lingli mu Peng Yu School of Pharmaceutical Sciences Central South University The Third Xiangya Hospital Central South University Hunan Tiger-Xiangya R&D Company Ltd. Medical College Hunan Normal University

The aim of this study was to apply the reference-scaled average bioequivalence (RSABE) approach to evaluate the bioequivalence of 2 formulations of agomelatine, and to investigate the pharmacokinetic properties of agomelatine in Chinese healthy male subjects. This was performed in a single-dose, randomized-sequence, open-label, four-way crossover study with a one-day washout period between doses. Healthy Chinese males were randomly assigned to receive 25 mg of either the test or reference formulation. The formulations were considered bioequivalent if 90% confidence intervals (CIs) for the log-transformed ratios and ratio of geometric means (GMR) of AUC and C m of agomelatine were within the predetermined bioequivalence range based on RSABE method. Results showed that both of the 90% CIs for the log-transformed ratios of AUC and C-max of 7-desmethyl-agomelatine and 3-hydroxyagomelatine were within the predetermined bioequivalence range. The 90% CIs for natural log transformed ratios of C-max, AUC(0-t), and AUC(0-infinity) of agomelatine (104.42-139.86, 101.33-123.83 and 97.90-117.94) were within the RSABE acceptance limits, and 3-hydroxy-agomelatine (105.55-123.03, 101.95-109.10 and 101.72-108.70) and 7-desmethyl-agomelatine (104.50-125.23, 102.36-111.50 and 101.62-110.64) were within the FDA bioequivalence definition intervals (0.80-1.25 for AUC and 0.75-1.33 for C-max). The RSABE approach was successful in evaluating the bioequivalence of these two formulations. (C) 2016 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V.

关键词： Reference-scaled average bioequivalence Agamelatine 3-Hydroxy-agomalatine 7-Desmethyl-agomelatine Chinese subjects High variability Generic drug

Preliminary results of FeO mapping using Imaging Interferometer data from Chang'E-1

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Chinese Science Bulletin 2011年第4期56卷 376-379页

作者： LING ZongCheng ZHANG Jiang LIU JianZhong ZHANG WenXi BIAN Wei REN Xin mu lingli LIU JianJun LI ChunLai National Astronomical Observatories Chinese Academy of Sciences Beijing 100012 China School of Space Science and Physics & Shandong Provincial Key Laboratory of Optical Astronomy and Solar-Terrestrial Environment Shandong University at Weihai Weihai 264209 China Academy of Opto -Electronics Chinese Academy of Sciences Beijing 100080 China

Information about the variability,and spatial distribution of iron abundance is important to understand lunar geological history and for future resource utilization. In this paper we present a preliminary model to produce an iron abundance map using images taken by an Imaging Interferometer on board the satellite Chang'E-1. Compared with the Clementine UVVIS images,the images from the Chang'E-1 satellite also allowed for the extraction of FeO abundance distributions on the Moon. However,the prelimi-nary model results suggest an underestimation of ～2 wt.% for the FeO content of the mare region and an overestimation of ～3 wt.% for the highland region.

关键词： FeO含量嫦娥一号干涉仪像数映射资源利用率空间分布地质历史

Preliminary results of TiO_2 mapping using Imaging Interferometer data from Chang'E-1

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Chinese Science Bulletin 2011年第20期56卷 2082-2087页

作者： LING ZongCheng ZHANG Jiang LIU JianZhong ZHANG WenXi ZHANG GuangLiang LIU Bin REN Xin mu lingli LIU JianJun LI ChunLai National Astronomical Observatories Chinese Academy of SciencesBeijing 100012China School of Space Science and Physics & Shandong Provincial Key Laboratory of Optical Astronomy & Solar-Terrestrial Environment Shandong University at WeihaiWeihai 264209China Academy of Opto-Electronics Chinese Academy of SciencesBeijing 100080China

The distribution of titanium abundance on the lunar surface is important knowledge for lunar geologic studies and future resource *** this paper,we develop a preliminary model based on"ground truths"from Apollo and Luna sample-return sites to produce a titanium abundance map from Chang’E-1 Imaging Interferometer(IIM) *** derived TiO2 abundances are validated with Clementine UVVIS results in several regions,including lunar highlands neighboring the Apollo 16 landing site,and high-Ti and low-Ti maria near the standard site of Mare Serenitatis(MS2) .The validation results show that TiO2 abundances modeled with Chang’E-1 IIM data are overestimated for highlands(～0.7 wt.%) and low-Ti maria(～1.5 wt.%) and underestimated for high-Ti maria(～0.8 wt.%).

关键词：二氧化钛干涉仪嫦娥一号成像映射月球表面资源利用率玛丽亚

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月表空间环境因子动态建模驱动的探测区选取

武汉大学学报（信息科学版） 2017年第1期42卷 91-96页

作者：曾兴国牟伶俐中国科学院国家天文台北京100012 中国科学院月球与深空探测重点实验室北京100012

针对在复杂的月表环境下及时高效地选择合适的探测区域这一问题,考察已有的月球探测研究资料,分析了可能影响到科学探测的月球空间环境因子,在此基础上开发了一个可对环境因子动态建模并应用模型自动选取探测区的GIS系统。基于该系统,... 详细信息

针对在复杂的月表环境下及时高效地选择合适的探测区域这一问题,考察已有的月球探测研究资料,分析了可能影响到科学探测的月球空间环境因子,在此基础上开发了一个可对环境因子动态建模并应用模型自动选取探测区的GIS系统。基于该系统,构建了一个针对嫦娥三号着陆区附近探测区域选取的评价模型,并将其成功应用于嫦娥三号巡视器科学探测目标的选取工作。

关键词：嫦娥三号月球探测月球环境因子月球影像地图

基于Java手机的野外农田数据采集与传输系统设计

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农业工程学报 2006年第11期22卷 165-169页

作者：牟伶俐刘钢黄健熙中国科学院国家天文台中国科学院遥感应用研究所北京100101 华中科技大学计算机科学学院中国科学院遥感应用研究所

为了解决“数字农业”中对野外农田数据采集传输的机动性,跨平台与经济性,在分析当前农田数据野外采集现状的基础上,提出基于Java手机平台野外农田数据采集与传输系统的设计方案。在B/S网络构架下,详细分析客户端与服务端系统设计客户... 详细信息

为了解决“数字农业”中对野外农田数据采集传输的机动性,跨平台与经济性,在分析当前农田数据野外采集现状的基础上,提出基于Java手机平台野外农田数据采集与传输系统的设计方案。在B/S网络构架下,详细分析客户端与服务端系统设计客户端设计主要包括农田数据采集、数据传输、信息查询、定位导航;服务端设计主要包括数据接收与传输,数据存取,数据检索以及地图服务等。通过试验,结果表明基于Java手机的野外农田数据采集与传输系统设计切实可行,在野外数据采集方面具有良好的移植性,数据传输较快,费用较低,具有较大的研究与实用价值。

关键词： Java手机,农田数据数据采集数据传输 J2ME

Data Analysis of Chang'E-1 Gamma-Ray Spectrometer and Global Distribution of U,K,and Th Elemental Abundances

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Acta Geologica Sinica(English Edition) 2011年第6期85卷 1299-1309页

作者： ZOU Yongliao ZHANG Liyan LIU Jianzhong mu lingli REN Xin ZHANG Guangliang CHANG Jin YAN Jun ZHANG Nan ZHANG Hongbo LU Chang LIU Jianjun ZUO Wei SU Yan WEN Weibin BIAN Wei WANG Min XU Chun LI Chunlai OUYANG Ziyuan National Astronomical Observatories the Chinese Academy of Sciences Beijing 100012 China Purple Mountain Observatory the Chinese Academy of Sciences Nanjing 210008 China

Gamma-ray spectrometer （GRS） is one of the main payloads on the Chang＇E-1 （CE-1） lunar probe, mainly aimed to detect the elemental abundances and distributions on the lunar surface. At 03：58 on 28 November 2007, it performed the first observation of the lunar gamma rays. As of 24 October 2008, 2105 h of effective gamma rays spectra had been acquired by CE-1 GRS, which covers the whole surface of the moon. This paper mainly describes the data processing procedures and methods of deriving the elemental abundances by using the CE-1 GRS time series corrected spectra： first, to bin data into pixels for mapping; then, to perform a background deduction of the cumulative spectra and obtain a peak area of the elements; and finally, to use the elemental abundances inversion model to produce the elemental abundances. Based on these processing methods, the global abundance maps of U, K, and Th at a 5°×5° equal-area pixel are acquired by CE-1 GRS data. The paper gives a preliminary analysis of the uncertainties of the elemental abundances.

关键词： Chang'E-1 payloads gamma rays elemental abundances

利用嫦娥三号全景相机数据构建沉浸式虚拟月表环境系统

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计算机辅助设计与图形学学报 2016年第3期28卷 526-532页

作者：高兴烨刘建军任鑫牟伶俐李春来中国科学院国家天文台月球与深空探测研究部北京100012 中国科学院大学北京100049 中国科学院月球与深空探测重点实验室北京100012

为辅助月球科学家在嫦娥三号巡视探测任务中全面了解巡视器周围的探测环境,利用嫦娥三号巡视器全景相机获取的双目立体图像以及天文星表数据,基于四通道立体投影设备,构建了沉浸式虚拟月表环境系统.介绍了所实现的虚拟月表环境系统的系... 详细信息

为辅助月球科学家在嫦娥三号巡视探测任务中全面了解巡视器周围的探测环境,利用嫦娥三号巡视器全景相机获取的双目立体图像以及天文星表数据,基于四通道立体投影设备,构建了沉浸式虚拟月表环境系统.介绍了所实现的虚拟月表环境系统的系统功能、系统结构及构建流程,并对虚拟月表环境系统的真实性建模、重投影算法、"沉浸式"实现等关键技术进行了详细的介绍.实际应用表明,该系统不但出色地在嫦娥三号任务中完成任务,而且还在后续科普活动中发挥了重要作用.

关键词：嫦娥三号全景相机沉浸式虚拟环境