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Prevalence and Spatial Distribution of Badnavirus in the Banana (Musa spp) Major Growing Areas in Burkina Faso

American Journal of Plant Sciences 2023年第4期14卷 427-447页

作者： Bakary Ouattara Drissa Sérémé moustapha koala Léon Wêndé-m’minèré Nitiéma Kadidia Koïta Emmanuel Kaboré Issa Wonni Laboratoire de Protection et Défense des Cultures Institut de l’Environnement et de Recherches Agricoles Centre National de Recherche Scientifique et Technologique Ouagadougou Burkina Faso Laboratoire de Biosciences Département de Biologie et Physiologie Végétale Université Joseph KI-ZERBO Ouagadougou Burkina Faso Laboratoire Central d’horticulture Institut de l’Environnement et de Recherches Agricoles Centre National de Recherche Scientifique et Technologique Bobo Dioulasso Burkina Faso

Banana streak virus (BSV) and Sugarcane bacilliform virus (SCBV) are two badnaviruses commonly found in all banana growing areas of the world. It is a threat to the production and improvement of Musa germplasm. In Burkina Faso, the presence of badnaviruses was reported in banana producing regions. The objective of this study was to determine the prevalence of BSV and SCBV in banana production areas of Burkina Faso. A survey followed by a symptomatologic study was conducted in banana plantations in 27 localities of the nine main banana producing regions from July to October 2018 and September to December 2020. In all, 251 leaf samples were collected and analysed for BSV and SCBV infection by Indirect Antigen Coated Plate Assay-ELISA followed by amplification of the RT/RNase H region using Polymerase chain reaction with Badna FP/RP and SCBV F/R primers, respectively. A variety of symptoms were observed on almost all plant organs which were revealed due to BSV by symptomatologic study. The results of serological and molecular diagnosis revealed a high overall prevalence of BSV in 80.48% of the samples tested. BSV was distributed in seven survey regions out of nine with prevalence ranging from 10% to 100% in North, Centre, Centre West, Hauts Bassins, Cascades, Centre East and Boucle of Mouhoun regions. Very low prevalence was recorded for SCBV in Cascades and East Centre region with 4.35 and 12.5%, respectively. Species detection using specific primers to each species revealed three main species: Banana streak Obino l’ewaï virus (BSOLV), Goldfinger virus (BSGFV) and Imové virus (BSIMV) in the samples tested, respectively in the proportions of 23%, 8% and 0.8%. Co-infection between BSV species was also detected.

关键词： Banana Streak Virus Sugarcane Bacilliform Virus Indirect Antigen Coated Plate Assay Polymerase Chain Reaction Musa spp

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<i>Imperata yellow mottle virus</i>: An Emerging Threat to Maize, Sorghum and Pearl Millet in Burkina Faso

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Agricultural Sciences 2017年第5期8卷 397-408页

作者： moustapha koala Valentin Stanislas Edgar Traoré Drissa Sérémé Bouma James Neya Christophe Brugidou Nicolas Barro Oumar Traoré Laboratoire Mixte Internationale (LMI) Patho-Bios Institut de l’Environnement et de Recherches Agricoles (INERA) Ouagadougou Burkina Faso Universite Ouaga I Professeur Joseph Ki-Zerbo Ouagadougou Burkina Faso UMR IPME (UM IRD CIRAD) Institut de Recherche pour le Développement IRD Montpellier France

Imperata yellow mottle caused by Imperata yellow mottle virus (IYMV) of the genus Sobemovirus was first characterized on Imperata cylindrical and Zea mays in Burkina Faso. The disease has been reported in several localities of the country but its ecology and epidemiology are poorly known. In particular, only I. cylindrical and maize have been reported within IYMV host range. The aim of this study was to investigate the experimental host range of the virus. Mechanical inoculation of a mixture of four IYMV isolates to 18 plant species, including four crops (maize, rice, sorghum and pearl millet) and 14 wild grasses showed clear mottle symptoms in maize, sorghum and pearl millet and two wild grass species (Setaria verticillata and Rottboellia exaltata). Symptom development was confirmed by Enzyme-linked immunosorbent assay and reverse transcription–polymerase chain reaction (RT-PCR). Infection of crop species by IYMV depended on cultivars tested. Therefore, proportions of positive cultivars were 16/36 in maize, 4/10 in sorghum and 4/9 in pearl millet, respectively. Studies on virus-host interactions using individual virus isolates showed two pathogenic patterns. Three out of the four isolates tested infected all plant species and cultivars. In contrast, the fourth IYMV isolate could infect only one maize cultivar. These results expand the previously known host range of IYMV from two to five species, indicating a narrow host range. Among the new characterized host species, sorghum and pearl millet are important cereal crops. Therefore, Imperata yellow motte disease is a potential threat for the cereal crop production and its ecology and epidemiology should be thoroughly investigated.

关键词： IYMV Sobemovirus Host Range Virus-Host Interactions

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Molecular Variability and Genetic Structure of IYMV in Burkina Faso

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American Journal of Plant Sciences 2018年第2期9卷 311-324页

作者： moustapha koala Drissa Sérémé Florence Vignols Eric Lacombe Martine Bantgratz Bouma James Neya Christophe Brugidou Nicolas Barro Oumar Traoré Laboratoire de Virologie et de Biotechnologie Végétale (LVBV) Institut de l’Environnement et de Recherches Agricoles (INERA) Ouagadougou Burkina Faso Laboratoire Mixte Internationale (LMI) Patho-Bios Institut de l’Environnement et de Recherches Agricoles (INERA) Ouagadougou Burkina Faso UMR IPME (UM IRD CIRAD) Institut de Recherche pour le Développement (IRD) Montpellier Cedex France Université Ouaga I Professeur Joseph Ki-Zerbo Ouagadougou Burkina Faso

Imperata yellow mottle virus (IYMV, Sobemovirus) was first described in 2008 in the south-western region of Burkina Faso (West Africa). The genetic diversity of IYMV was not documented up to day. In this study, the variability of CP of IYMV was evaluated through the molecular characterization of 38 isolates collected in the western part of Burkina Faso. Comparison of sequences of these new isolates and one IYMV sequence available in GenBank revealed that the average nucleotide diversity was low. The ratio of non-synonymous over synonymous nucleotide substitutions per site was low, indicating a CP diversification under strong purifying selection. Despite of the low nucleotide diversity, phylogenetic analyses revealed segregation of IYMV isolates into six major clades. There was no correlation of phylogenetic grouping of isolates based on geographical location. This is the first study of the genetic diversity of IYMV.

关键词： Imperata yellow mottle virus (IYMV) Coat Protein (CP) Genetic Var-iability Phylogenetic Analysis

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Molecular Characterization of a Large Diversity of Bambara Groundnut Potyviruses (<i>Vigna subterranea</i>(L.) Verdc.) in Burkina Faso

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Advances in Bioscience and Biotechnology 2019年第9期10卷 270-281页

作者： Aboubié Elisabeth Zongo Bouma James Néya Essowé Palanga Drissa Sérémé moustapha koala Régis Dimitri Sokpé Longué Jean Zabré Zakaria Bouda Nicolas Barro Oumar Traoré Laboratoire de Virologie et de Biotechnologie Végétale (LVBV)/Institut de l’Environnement et de Recherches Agricoles (INERA) & Laboratoire Mixte Internationale (LMI) Patho-Bios/INERA Ouagadougou Burkina Faso Université Ouaga I Professeur Joseph Ki-Zerbo Ouagadougou Burkina Faso Laboratoire National de Biosécurité Ouagadougou Burkina Faso

Potyviruses are major constraints to grain legume production by causing significant yield losses. Potyviruses infecting Bambara groundnut (Vigna subterranea) were investigated in Burkina Faso. Leaf samples collected from three agroclimatic zones were subjected to RT-PCR and sequence analyses. Of a total of 135 samples, 36 (26.67%) were detected positive in RT-PCR tests using potyvirus universal primers. Analysis of full coat protein (cp) sequences from 24 isolates revealed the occurrence of three groups of Bambara groundnut-infecting potyviruses. Virus isolates in group 1 shared 94.5% - 100% nucleotide (nt) identity with CABMV whereas those in group 2 and group 3 were distantly related Bean common necrosis virus (BCMNV) and Passiflora virus Ugandan which were their respective closest potyviruses. Group 2 shared 77.1% nt and 78.8% - 79.9% aa identity with BCMNV and group 3 shared 77.3% - 78.3% nt and 80.7% - 81.5% aa identity with Passiflora virus Ugandan. All three groups were confirmed by phylogenetic analyses. Taking into account potyvirus demarcation criteria, group 1 isolates belonged to CABMV species. Group 2 and group 3 were assigned to a potentially new potyviruses species and designated Bambara groundnut potyvirus 1 (BGPV1) and Bambara groundnut potyvirus 2 (BGPV2).

关键词： Bambara Groundnut Potyvirus Characterization Coat Protein Sequence Diversity

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