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Effect of Nonenzymatic Antioxidants on Sperm Motility and Survival Relative to Free Radicals and Antioxidant Enzymes of Chilled-Stored Ram Semen

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Open Journal of Animal Sciences 2015年第1期5卷 50-58页

作者： moustafa M. zeitoun Mona A. Al-Damegh Department of Animal Production and Breeding College of Agriculture and Veterinary Medicine Qassim University Buriedah KSA Department of Biology College of Sciences and Arts Qassim University Oniza KSA

The effect of inclusion of three antioxidants (Vitamin E, cysteine and glutathione) in ram semen extender on the release of antioxidant enzymes and free radicals was studied. A 3 × 3 factorial experiment was conducted to test effects of supplementing ram semen extender with Vitamin E (1, 5 and 10 IU), cysteine (1, 5 and 10 mM) or glutathione (0.5, 1 and 2 mM) on the sperm survival and release of superoxide dismutase (SOD), glutathione peroxidase (GPX) and thiobarbituric acid reactive substances (TBARS). Eighty ejaculates of eight fertile Najdi rams were collected, assessed for the gross examination and the good ejaculates (≥90% motility) were pooled and sperm count was assessed. Therefore ten extenders;control (C) and 9 treated (C plus antioxidant) were tested. Extended semen was stored at 5°C for 96 h, examined for motility and survival and sperm cells were separated from plasma, sonicated, homogenized and exposed to the determinations of SOD, GPX and TBARS. The highest sperm survival was found in diluents containing 5 IU Vitamin E/ml, 1 and 2 mM glutathione (55.5% survival), while the lowest survival was found in 10 mM cysteine (11.1%). TBARS concentration was highest (P < 0.05) in control than other treatments, however, Vitamin E and glutathione exhibited low values. Contrariwise, activities of SOD and GPX increased (P < 0.05) within sperm cells and seminal plasma in diluents containing 5 IU E, 1 mM and 2 mM glutathione. The enzymatic activities were generally higher in seminal plasma than in sperm cells. It was concluded that supplementing ram semen extender during chilled storage with 5 IU Vitamin E per ml or 1 - 2 mM glutathione enhanced sperm survival and reduced free radicals.

关键词： Ram Semen Storage Free Radicals Antioxidant Enzymes

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Impact of the aqueous extract of dandelion, probiotic and their synbiotic on male lamb’s testicular histopathology relative to semen characteristics

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Open Journal of Animal Sciences 2014年第1期4卷 23-30页

作者： moustafa zeitoun Mohamed Farahna Khaled Al-Sobayil Ahmed Abdel-Salam College of Applied Medical Sciences Qassim University Qassim KSA Department of Animal Production and Breeding Qassim University Buriedah KSA Department of Food Sciences and Human Nutrition College of Agriculture and Veterinary Medicine Qassim University Buriedah KSA

This study aimed at investigating effects of dandelion/probiotic on the testis histology and semen characteristics of male lambs. Twelve prepuberal Noemi male lambs (2 ± 0.4-month-old and 20 ± 3.5 kg B.W) were randomly allocated into four groups. Animals in group 1 were controlled, orally given normal saline as the same schedule of treated animals. In group 2 animals were given daily oral 50 ml of an aqueous extract of dandelion leaves. In group 3 animals were given a daily 50 ml of cow’s milk probiotics, and in group 4 animals were given 50 ml of a 1:1 mixture (dandelion extract: probiotic). Animals were treated for 8 consecutive weeks. At puberty, semen ejaculates were collected weekly for further 10 weeks. Thereafter, animals were sacrificed and testicles were obtained, weighed and a histopathological study was performed on the testis. Results revealed that oral administration of the dandelion aqueous extract to prepuberal lambs exhibited disturbances in the histological architecture of the testicular semeniferous tubules. Additionally, dandelion resulted in edematic interstitial tissues with evident vaculations. These deteriorations were reflected in less spermatogenesis (i.e. less sperm concentration and ejaculate volume by 10% and 20%, respectively than controlled group). Also, the percentage of sperm progressive motility was lower (P < 0.05) and dead sperm was higher (P < 0.05) in rams given dandelion than control. Administration of probiotic hasn’t revealed a change in the seminiferous tubules architecture, however, very little vaculation with rare edema was observed. Administration of the synbiotic mixture revealed intermediate histopathological appearances. The highest sperm concentration, testosterone concentration and advanced motility were obtained when probiotic was administered. In conclusion, although dandelion cures some hepatic malfunctions in human, caution must be taken into consideration of the frequent ingestion of dandelion for its harmful eff

关键词： Dandelion Histopathology Probiotic Ram Semen Testis

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L-Arginine Modulates Maternal Hormonal Profiles and Neonatal Traits during Two Stages of Pregnancy in Sheep

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Open Journal of Animal Sciences 2016年第2期6卷 95-104页

作者： moustafa zeitoun Ahmed Al-Ghoneim Khaled Al-Sobayil Suliman Al-Dobaib Department of Animal Production and Breeding College of Agriculture and Veterinary Medicine Qassim University Al-Qassim KSA Ministry of Agriculture Riyadh KSA

A 2 × 2 factorial arrangement was designed to test effects of supplementation of a low (L, 75 mg/kg BW) vs. high (H, 150 mg/kg BW) L-arginine given at early (first 56 days) vs. late (last 56 days) pregnancy on maternal hormones and neonatal traits. Thirty Najdi pregnant ewes were randomly allocated into 6 groups. Ewes in G1 and G2 served as controls (C), given 50 ml saline at either early (CE) or late (CL) pregnancy, respectively. G3 and G4 ewes in early pregnancy received low (LE) and high L-arginine (HE), respectively. G5 and G6 ewes in late pregnancy received low (LL) and high (HL) L-arginine, respectively. A weekly blood sample was collected from initiation of the treatment till parturition. Serum growth hormone (GH), insulin-like growth factor-I (IGF-I), insulin, progesterone (P4) and estradiol 17 β (E2) profiles were determined. Neonatal traits were also determined. Insulin was higher (P 0.05). Lamb survival rates at birth in LE ewes were highest (100%) compared to other treatments. In conclusion, supplementing pregnant ewes with low dosage of L-arginine at early stage of gestation increased lamb birth weight and survival, and improved maternal health.

关键词： L-Arginine Najdi Ewes Gestation Hormones Neonate

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Involvement of Quebracho tannins in diet alters productive and reproductive efficiency of postpartum buffalo cows

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Animal Nutrition 2019年第1期5卷 80-86页

作者： Sobhy M.A.Sallam Marwa F.A.Attia Adel N.M.Nour El-Din Samir Z.El-Zarkouny Adel M.Saber Hani M.El-Zaiat moustafa M.zeitoun Department of Animal and Fish Production Faculty of AgricultureAlexandria University Animal Production Research Institute Agricultural Research CenterDokki

This study was conducted to investigate the effects of 10 weeks supplementation of Quebracho tannins(QT; 0 [control], 100 [QT100] or 200 g/[cow$d] [QT_(200)]) to 30 multiparous postpartum buffalo cows(10 cows per group) on milk yield and composition, blood metabolites and reproductive *** of QT100 had no significant effect on milk yield, whereas QT_(200) decreased(P < 0.05) this trait. Compared with the control group, both QT levels decreased(P < 0.05) fat-corrected milk(FCM)yield, but no significant effects were found on percentages of milk fat and protein. Contrariwise, yields of milk fat, lactose and milk protein were decreased(P < 0.05) when QT_(200) was supplemented. The solids nonfat(SNF) percentage and yield were decreased(P < 0.05) with QT100 supplementation. Moreover, QT tended to numerically reduce total number of ovarian follicles, number of small follicles, peripheral progesterone concentration and conception rate. Supplementation of QT_(200) numerically increased number of large follicles, mean diameter of large follicle, number and diameters of corpora lutea. The inclusion of QT_(200) shortened days open(DO) and decreased number of services per ***, QT did not show significant effects on serum total protein, albumin, globulin, glucose,cholesterol and triglycerides concentrations. Supplementation of QT100 caused an increase(P < 0.05) of serum urea compared with that in control and QT_(200) groups. Generally, QT decreased(P < 0.05) serum creatinine concentration. Therefore, the supplementation of a commercial QT to early lactating Egyptian buffalo cows displayed negative consequences on their productive and reproductive performances.

关键词： Buffalo Tannins Milk traits Blood metabolites Ovarian follicles Conception rate

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Calculation of ship motions in steep waves with restoring and Froude-Krylov forces on an adaptive panel mesh with Gauss and analytic integration methods

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Journal of Hydrodynamics 2024年第2期36卷 275-289页

作者： Malwin Wermbter moustafa Abdel-Maksoud Institute for Fluid Dynamics and Ship Theory Hamburg University of TechnologyHamburgGermany

The impulse response method is a frequently used method to calculate ship seakeeping *** this paper,the restoring and Froude-Krylov calculation is conducted with constant evaluation of panel pressures as well as Gauss quadrature and an analytical *** applied panel grid is coarsened by an adaptive algorithm which is based on a normal vector *** comparison of methods is based on grid convergence studies which are followed by a verification of forces with computational fluid dynamics(CFD)results on the fixed duisburg test case in *** with experimental results in head,oblique and following waves show that all integration methods are *** exact integration is numerically sensitive in some *** quadrature is highly accurate;however,the additional effort is not beneficial since the geometrical accuracy has-stronger influence on the force amplitudes than the integration *** grid coarsening reduces the simulation time and is accurate up to a level,where the panel length comes close the *** added resistance at the investigated Froude number of 0.05 shows higher uncertainty levels,this applies to the results of both the numerical methods and model tests.

关键词： Ship hydrodynamics seakeeping impulse response method added resistance wave loads

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Feature extraction for machine learning-based intrusion detection in IoT networks

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Digital Communications and Networks 2024年第1期10卷 205-216页

作者： Mohanad Sarhan Siamak Layeghy Nour moustafa Marcus Gallagher Marius Portmann University of Queensland BrisbaneQLD4072Australia University of New South Wales CanberraACT2612Australia

A large number of network security breaches in IoT networks have demonstrated the unreliability of current Network Intrusion Detection Systems(NIDSs).Consequently,network interruptions and loss of sensitive data have occurred,which led to an active research area for improving NIDS *** an analysis of related works,it was observed that most researchers aim to obtain better classification results by using a set of untried combinations of Feature Reduction(FR)and Machine Learning(ML)techniques on NIDS ***,these datasets are different in feature sets,attack types,and network ***,this paper aims to discover whether these techniques can be generalised across various *** ML models are utilised:a Deep Feed Forward(DFF),Convolutional Neural Network(CNN),Recurrent Neural Network(RNN),Decision Tree(DT),Logistic Regression(LR),and Naive Bayes(NB).The accuracy of three Feature Extraction(FE)algorithms is detected;Principal Component Analysis(PCA),Auto-encoder(AE),and Linear Discriminant Analysis(LDA),are evaluated using three benchmark datasets:UNSW-NB15,ToN-IoT and *** PCA and AE algorithms have been widely used,the determination of their optimal number of extracted dimensions has been *** results indicate that no clear FE method or ML model can achieve the best scores for all *** optimal number of extracted dimensions has been identified for each dataset,and LDA degrades the performance of the ML models on two *** variance is used to analyse the extracted dimensions of LDA and ***,this paper concludes that the choice of datasets significantly alters the performance of the applied *** believe that a universal(benchmark)feature set is needed to facilitate further advancement and progress of research in this field.

关键词： Feature extraction Machine learning Network intrusion detection system IoT

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Privacy-preserved learning from non-i.i.d data in fog-assisted IoT:A federated learning approach

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Digital Communications and Networks 2024年第2期10卷 404-415页

作者： Mohamed Abdel-Basset Hossam Hawash Nour moustafa Imran Razzak Mohamed Abd Elfattah Faculty of Computers and Informatics Zagazig UniversityZagazigSharqiyah44519Egypt School of Engineering and Information Technology University of New South Wales@ADFACanberraACT2600Australia Deakin University Geelong Waurn Ponds CampusAustralia Computer Science Department Misr Higher Institute for Commerce and ComputersMansoura35511Egypt

With the prevalence of the Internet of Things(IoT)systems,smart cities comprise complex networks,including sensors,actuators,appliances,and cyber *** complexity and heterogeneity of smart cities have become vulnerable to sophisticated cyber-attacks,especially privacy-related attacks such as inference and data poisoning *** Learning(FL)has been regarded as a hopeful method to enable distributed learning with privacypreserved intelligence in IoT *** though the significance of developing privacy-preserving FL has drawn as a great research interest,the current research only concentrates on FL with independent identically distributed(i.i.d)data and few studies have addressed the non-i.i.d *** is known to be vulnerable to Generative Adversarial Network(GAN)attacks,where an adversary can presume to act as a contributor participating in the training process to acquire the private data of other *** paper proposes an innovative Privacy Protection-based Federated Deep Learning(PP-FDL)framework,which accomplishes data protection against privacy-related GAN attacks,along with high classification rates from non-i.i.d ***-FDL is designed to enable fog nodes to cooperate to train the FDL model in a way that ensures contributors have no access to the data of each other,where class probabilities are protected utilizing a private identifier generated for each *** PP-FDL framework is evaluated for image classification using simple convolutional networks which are trained using MNIST and CIFAR-10 *** empirical results have revealed that PF-DFL can achieve data protection and the framework outperforms the other three state-of-the-art models with 3%–8%as accuracy improvements.

关键词： Privacy preservation Federated learning Deep learning Fog computing Smart cities

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Impact of Quebracho Tannins Supplementation on Productive and Reproductive Efficiency of Dairy Cows

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Open Journal of Animal Sciences 2016年第4期6卷 269-288页

作者： Marwa F. A. Attia Adel N. M. Nour El-Din Samir Z. El-Zarkouny Hani M. El-Zaiat moustafa M. zeitoun Sobhy M. A. Sallam Department of Animal and Fish Production Faculty of Agriculture Alexandria University Alexandria Egypt Department of Arid Land Agriculture Faculty of Meteorology Environment and Arid Land Agriculture Jeddah Saudi Arabia Department of Animal Production and Breeding College of Agriculture and Veterinary Medicine Qassim University Qassim Saudi Arabia

This study was conducted to investigate effects of supplementing two doses of quebracho tannins (QT;100 or 200 g/cow/d;QT100 or QT200) pre and post parturition to thirty Holstein dairy cows on Dry Matter Intake (DMI), milk yield and composition, blood metabolites and reproductive performance for 12 weeks. There were no significant changes in DMI due to QT at transition period of dairy cows. QT supplementation at either level decreased (P 0.05) the total number of ovarian follicles, number of large follicles, diameter of largest follicle, number of small follicles, number and diameter of corpus luteum, and progesterone concentration. The inclusion of QT increased days open and number of services per conception, which consequently decreased conception rate, compared to the control cows. QT100 decreased (P

关键词： Quebracho Tannin Feed Intake Milk Yield Follicular Dynamics Conception Rate

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Solute transport and geochemical modeling of the coastal quaternary aquifer, Delta Dahab Basin, South Sinai, Egypt

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Acta Geochimica 2024年第1期43卷 16-39页

作者： Amira Samy Mustafa Eissa Seham Shahen moustafa M.Said Rabab M.Abou-shahaba Division of Water Resources and Arid Land Department of HydrogeochemistryDesert Research CenterCairoEgypt Egypt Desalination Research Center of Excellence Desert Research CenterCairoEgypt Center for Water Supply Studies S&E-Physical&Environmental SciencesTexas A&M University Corpus ChristiCorpus ChristiUSA Department of Chemistry Faculty of Science(Girls)Al-Azhar UniversityYousef Abbas StrNasr CityP.O.Box:11754CairoEgypt

The wadi dahab delta is in a dry, arid coastal zone within Egypt’s south Sinai Peninsula’s eastern portion. The primary water source is the Quaternary coastal alluvial aquifer. The groundwater salinity varies from 890to 8213 mg/L, with a mean value of 3417 mg/L. The dissolved major ions have been used to calculate the seawater mixing index(SWMI) using a linear equation that discriminates the groundwater mostly affected by water–rock interaction(SWMI 1>) and other samples mixed with Seawater(SWMI < 1). The isotopic composition of groundwater for specifically chosen groundwater samples ranges from-0.645‰ to +5.212‰ for δ^(18)O and from-9.582‰ to + 22.778‰ for δ^(2)H, where the seawater represented by a Red Sea water sample(δ^(18)O + 1.64‰-δ^(2)H + 9.80‰) and reject brine water are considerably enriched the isotopic groundwater values. The geochemical NETPATH model constrained by the dissolved significant ions, isotopes, and the rock aquifer forming minerals as phases indicate the mixing percent with the seawater ranges from 9% to 97% of seawater from 91% to 3% of original recharge water. According to the SEAWAT 3-D flow models, seawater has penetrated the Northeastern Dahab delta aquifer, with the intrusion zone extending1500 m inland. The salt dissolution, upwelling of saline water, recharge from the upstream mountain block, and seawater encroachment are the primary aspects contributing to the deterioration of groundwater quality. These findings may have significance for effective groundwater withdrawal management in arid locations worldwide with similar hydrogeological systems.

关键词： Groundwater geochemistry Seawater intrusion Solute transport SEAWAT

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L-Arginine Supplementation Alters Maternal Blood Biochemical Attributes and Milk Composition Relative to Neonatal Traits of <i>Najdi</i>Ewes

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Open Journal of Animal Sciences 2019年第3期9卷 341-354页

作者： moustafa M. zeitoun Ahmed A. Al-Ghoneim Khalid A. Al-Sobayil Yasser R. Hassanein Mohamed M. Mansour Kheir El-Din M. El-Azrak Marwa F. A. Attia Tariq I. Almundarij Department of Animal Production and Breeding College of Agriculture and Veterinary Medicine Qassim University Buriedah KSA Department of Animal and Fish Production Faculty of Agriculture Alexandria University El-Shatby Egypt Ministry of Agriculture Riyadh KSA Department of Hematology College of Applied Medical Sciences Qassim University Buriedah KSA Department of Animal Production Faculty of Agriculture Damanhur University Damanhur Egypt Department of Veterinary Medicine College of Agriculture and Veterinary Medicine Qassim University Buriedah KSA

In a 2 × 3 factorial arrangement, 3 levels of L-arginine (0, 75 and 150 mg/kg BW per day) were tested at two stages of pregnancy (Early 60 days vs. Late 60 days) on the maternal blood metabolites and postnatal milk composition relative to the newborn traits of Najdi ewes. Thirty adult pregnant Najdi ewes were randomly assigned into 6 treatments (n = 5). Control early (CE) ewes were orally administered 50 mL normal saline (0.9% NaCl) daily during the first 60 days of pregnancy;control late (CL) ewes were similar to CE except saline was given at the last 60 days of pregnancy;low dose of L-arginine at early pregnancy (LE);low dose of L-arginine at late pregnancy (LL);high dose of L-arginine at early (HE) and high dose of L-arginine at late pregnancy (HL). Blood and milk samples were collected on a weekly basis. Data of lambing and newborn traits were considered. There were non-significant differences in litter size due to L-arginine;however lamb birth and weaning weights increased by 35% in LE-ewes. Arginine did not change blood protein and albumin. Low arginine decreased lipid fractions, creatinine, urea, and milk fat;however, it increased AST, ALT, milk lactose and milk ash. Administration of L-arginine at 75 mg/Kg BW per day for 60 days at an early gestation in ewes improved the maternal body health and milk composition for the welfare of the neonates.

关键词： L-Arginine Ewes Fetus Metabolites Lactation Offspring

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