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CRP Binding Kinetics Enhancement Using Local Narrowing into a bent Channel: Finite Element Analysis

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Engineering（科研） 2022年第1期14卷 62-75页

作者： Mohamed Bahri Imen Dermoul Marshet Getaye Mohamed Amin Elaguech Khouloud Djebbi mounir ben ali Chaker Tlili Deqiang Wang Chongqing School University of Chinese Academy of Sciences Chongqing China Chongqing Institute of Green and Intelligent Technology Chinese Academy of Sciences Chongqing China University of Chinese Academy of Sciences Beijing China Preparatory Institute for Engineering of Nabeul IPEIN University of Carthage Campus Universitaire Merazka Tunisia NANOMISENE Lab LR16CRMN01 Centre for Research on Microelectronics and Nanotechnology of Sousse Technopole of Sousse Sousse Tunisia University of Sousse Higher Institute of Applied Sciences and Technology of Sousse GREENS-ISSAT Cité Ettafala Sousse Tunisia

Binding kinetics enhancement of a microfluidic biosensor into a micro-channel through the application of a supplementary mechanism has received tremendous attention because of the obtained significant enhancement factor. However, biosensor’s performance enhancement using only simple channel engineering is still rarely realized. Herein, we present a novel design of a complex reactive protein (CRP) biosensor into a U-shaped channel with a sensitive membrane located in the middle of the bent zone. Various critical factors affecting the equilibrium binding time are numerically investigated. The turn geometry is then optimized when the arc length along the inner and outer radii is almost the same, which leads to locally minimizing the channel height overhead the reaction surface and improves the analyte transport towards the sensing area. The numerical studies reveal that applying a local narrowing above the reaction surface can notably enhance the trapping and the surface formation of complex antibody-antigen, thus upgrading the biosensor performance. This work puts a significant advance towards microfluidic channel engineering and the exploration of micro-flow injection experimental studies.

关键词： Finite Elements Method Binding Reaction CRP Biosensor Microfluidic System Microchannel Turn Numerical Simulation

MACS-annexin V cell sorting of semen samples with high TUNEL values decreases the concentration of cells with abnormal chromosomal content:a pilot study

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Asian Journal of Andrology 2022年第5期24卷 445-450页

作者： Sahar El Fekih Nadia Gueganic Corinne Tous Habib ben ali mounir Ajina Nathalie Douet-Guilbert Hortense Drapier Damien Beauvillard Frédéric Morel Aurore Perrin University of Brest InsermUMR U1078Faculty of Medicine and Health SciencesBrest 29238France Laboratory of Human Cytogenetics Molecular Genetics and Reproductive BiologyFarhat Hached University HospitalSousse 4031Tunisia Departement of Medical Genetics and Reproductive Biology Brest University Regional HospitalBrest 29609France Department of Reproductive Medicine Farhat Hached Teaching HospitalSousse 4031Tunisia

We question whether,in men with an abnormal rate of sperm DNA fragmentation,the magnetic-activated cell sorting(MACS)could select spermatozoa with lower rates of DNA fragmentation as well as spermatozoa with unbalanced chromosome *** spermatozoa from six males were separated into nonapoptotic and apoptotic *** determined the percentages of spermatozoa with(i)externalization of phosphatidylserine(EPS)by annexin V-Fluorescein isothiocyanate(FITC)labeling,(ii)DNA fragmentation by TdT-mediated-dUTP nick-end labeling(TUNEL),and(iii)numerical abnormalities for chromosomes X,Y,13,18,and 21 by fluorescence in situ hybridization(FISH),on the whole ejaculate and selected spermatozoa in the same *** to the nonapoptotic fraction,the apoptotic fraction statistically showed a higher number of spermatozoa with EPS,with DNA fragmentation,and with numerical chromosomal *** to the whole ejaculate,we found a significant decrease in the percentage of spermatozoa with EPS and decrease tendencies of the DNA fragmentation rate and the sum of disomy levels in the nonapoptotic ***,we observed statistically significant higher rates of these three parameters in the apoptotic *** may help to select spermatozoa with lower rates of DNA fragmentation and unbalanced chromosome content in men with abnormal rates of sperm DNA fragmentation.

关键词： chromosomal abnormalities DNA fragmentation externalization of phosphatidylserine magnetic-activated cell separation spermatozoa

Oxidative stress in male infertility and therapeutic approach: A mini-review

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Asian pacific Journal of Reproduction 2023年第6期12卷 249-255页

作者： Marwa Lahimer Henda Mustapha Véronique Bach Hafida Khorsi-Cauet Moncef benkhalifa mounir Ajina Habib ben ali Service of Reproductive Medicine University Hospital Farhat HachedSousseTunisia Exercise Physiology and Physiopathology:from Integrated to Molecular“Biology Medicine and Health”(Code:LR19ES09)SousseTunisia Péritox(UMR_I 01) UPJV/INERISUPJVCURSChemin de Thil 80025 AmiensFrance ART and Reproductive Biology Laboratory University Hospital and School of MedicinePicardie University Jules VerneCHU Sud80054AmiensFrance Laboratory Histology Embryology Faculty of Medicine SousseUniversity of SousseSousseTunisia

Growing evidence suggests that oxidative stress is a major cause of male *** are highly sensitive cells due to the vulnerability of their membrane fatty acids and the diminished antioxidant *** oxygen species(ROS)impact multiple signaling pathways involved in mitochondrial dysfunction,hormonal unbalance and semen quality *** origin of ROS is diverse,including generated normal cellular metabolism,environmental exposure,advanced paternal age and *** has indicated that antioxidant supplementation can improve oxidative stress and reduce the risk of chronic ***,it is widely known that antioxidant supplementation can enhance sperm quality and increase the pregnancy rate in couples undergoing fertility *** current study highlights the deleterious effect of ROS and its impact on semen *** addition,it contributes to the development of a clinical approach for the use of antioxidants in the management of male infertility.

关键词： Oxidative stress Reactive oxygen species Mitochondria Antioxidant supplementation Spermatozoa

Study of gonadic growth factors: Seminal transforming growth factor-β1, epidermal growth factor and insulin-like growth factor-I and their relationship with male infertility

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Advances in Reproductive Sciences 2014年第1期2卷 16-23页

作者： Houda Amor mounir Ajina ali Saad Habib ben ali Laboratoire de Cytogénétique Génétique Moléculaire et Biologie de la Reproduction Humaines CHU Farhat Hached Sousse Tunisie Unité de Médecine de la Reproduction CHU Farhat Hached Sousse Tunisie

Background: The aim of this study was to establish normal reference values for several growth factors in semen and provide a basis for understanding their physiological significance in fertile and infertile subjects. Methods: 110 infertile men semen was evaluated according to WHO standards and by enzyme immunoassay (ELISA kit protocol) to determine the rate of growth factors (TGFβ1, IGFI, EGF). We have also listed neutrophils by staining with peroxidase to examine the association of inflammation with the levels of these factors. The determination of the epididymal (alpha-1,4-glucosidase) marker was directed according to the technical recommended by the WHO in 2010. Each patient received a hormonal balance including the determination of plasma FSH, LH and Testosterone. Results: Concentrations of TGFβ1 were significantly higher in (severe and extreme azoospermic, oligospermic) pathological groups compared to the control group (P 0.05) between control and pathological groups. The IGFI is partly testicular and/or epididymal as it is lowered in some patients with obstructive azoospermia. Positive correlations were observed between the seminal IGFI, mobility (r = 0.487) and sperm morphology (r = +0.187). Immunological sperm abnormalities and/or idiopathic do not alter the rate of EGF seminal. There is no significant correlation between seminal EGF or with sperm parameters or with plasma levels of FSH and LH. Conclusion: The growth factors are primarily peptides, handling and identification in a complex environment as the seminal plasma, but it requires a lot of precautions if we do not want to expose to the risk of bad farms/interpretations of data obtained.

关键词： Infertility Semen EGF IGF-I TGF-β1 FSH LH

Dimethoate Induced Oxidative Damage and Histopathological Changes in lung of Adult rats:Modulatory Effects of Selenium and/or Vitamin E

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Biomedical and Environmental Sciences 2012年第3期25卷 340-351页

作者： Ibtissem ben AMARA Nejla SOUDANI Afef TROUDI Ahmed HAKIM Khaled mounir ZEGHAL Tahia BOUDAWARA Najiba ZEGHAL Animal Physiology Laboratory Faculty of ScienceBP11713000 SfaxUniversity of SfaxTunisia Laboratory of Pharmacology Faculty of Medicine3029 SfaxUniversity of Sfax Tunisi Anatomopathology Laboratory CHU Habib Bourguiba 3029 SfaxUniversity of SfaxTunisia

Objective To determine the efficiency of selenium and/or vitamin E to alleviate lung oxidative damage induced by dimethoate, an organophosphorus compound. Methods Adult Wistar rats were exposed during 30 days either to dimethoate （0.2 g/L of drinking water）, dimethoate＋selenium （0.5 mg/kg of diet）, dimethoate＋vitamin E （100 mg/kg of diet）, or dimethoate＋selenium＋vitamin E. Results Exposure to dimethoate caused oxidative stress in lung evidenced by an increase of malondialdehyde, protein carbonyl groups and advanced oxidation protein products. An increase in glutathione peroxidase, superoxide dismutase, catalase and a decrease in acetylcholinesterase and butyrylcholinesterase activities, glutathione, non-protein thiols and vitamins C levels were observed. Histopathological changes in lung tissue were noted as emphysema, hemorrhages and hemosiderin deposits. Co-administration of selenium or vitamin E to the diet of dimethoate treated rats ameliorated the biochemical parameters as well as histological impairments. The joint effect of these elements was more powerful in antagonizing dimethoate-induced lung oxidative damage. Conclusion We concluded that selenium and vitamin E ameliorated the toxic effects of this pesticide in lung tissue suggesting their role as potential antioxidants.

关键词： Dimethoate Selenium Vitamin E Rats Lung Antioxidant status Histopathological studies

Potassium Bromate-induced Changes in the Adult Mouse Cerebellum Are Ameliorated by Vanillin

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Biomedical and Environmental Sciences 2018年第2期31卷 115-125页

作者： Hajer ben Saad Dorra Driss Imen Jaballi Hanen Ghozzi Ons Boudawara Michael Droguet Christian Magne Monsef Nasri Khaled mounir Zeghal Ahmed Hakim Ibtissem ben Amara Laboratory of Pharmacology University of SfaxFaculty of Medicine3029 SfaxTunisia Laboratory of Enzyme engineering and Microbiology National Engineering School in SfaxUniversity of SfaxB.P.11733038 SfaxTunisia Centre of Biotechnology of Sfax P.O.Box 11773018 SfaxTunisia Anatomopathology Laboratory University of SfaxHabib Bourguiba Hospital3029 SfaxTunisia EA 4324 ORPHY Faculty of MedicineUniversity of Brest29238 Brest CedexFrance EA 2219 Geoarchitecture University of BrestUFR Sciences&Techniques29238 Brest Cedex 3France

Objective The current study aimed to elucidate the effect of vanillin on behavioral changes, oxidative stress, and histopathological changes induced by potassium bromate （KBrO3）, an environmental pollutant, in the cerebellum of adult *** The animals were divided into four groups： group 1 served as a control, group 2 received KBrO3, group 3 received KBrO3 and vanillin, and group 4 received only vanillin. We then measured behavioral changes, oxidative stress, and molecular and histological changes in the *** We observed significant behavioral changes in KBrO3-exposed mice. When investigating redox homeostasis in the cerebellum, we found that mice treated with KBrO3 had increased lipid peroxidation and protein oxidation in the cerebellum. These effects were accompanied by decreased Na＋-K＋ and Mg2＋ ATPase activity and antioxidant enzyme gene expression when compared to the control group. Additionally, there was a significant increase in cytokine gene expression in KBrO3-treated mice. Microscopy revealed that KBrO3 intoxication resulted in numerous degenerative changes in the cerebellum that were substantially ameliorated by vanillin supplementation. Co-administration of vanillin blocked the biochemical and molecular anomalies induced by *** Our results demonstrate that vanillin is a potential therapeutic agent for oxidative stress associated with neurodegenerative diseases.

关键词： Cerebellum Behavior Potassium bromate Vanillin ATPases Genes expression

Multiple pyogenic liver abscess

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World Journal of Gastroenterology 2006年第18期12卷 2962-2963页

作者： Mabrouk Bahloul Anis Chaari Nadia Bouaziz-Khlaf Hatem Kallel Leila Herguefi Hedi Chelly Chokri ben Hamida mounir Bouaziz Service de Réanimation médicale CHU Habib Bourguiba Route el Ain Km 1 3029 Sfax Tunisie

Multiple pyogenic liver abscesses have been rarely described. We report a fatal case of multiple pyogenic liver abscesses affecting a 38-year-old woman requiring surgical drainage. Evolution was marked by occurrence of a septic shock with multi-organ system failure. The patient died 48 h after surgery. Causes, therapeutics and outcome of the disease are discussed.

关键词： Liver abscess Septic shock Outcome

Haemophagocytic lymphohistiocytosis secondary to Plasmodium falciparum malaria: Case report and review of the literature

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Asian Pacific Journal of Tropical Medicine 2020年第10期13卷 467-471页

作者： Fatma Hammami Makram Koubaa Mourad Chaari Rim Chaabouni Abrakhom Zeyni Khaoula Rekik Fatma Smaoui mounir ben Jemaa Infectious Diseases Department Hedi Chaker University HospitalUniversity of SfaxTunisia Hematology Laboratory Hedi Chaker University HospitalUniversity of SfaxTunisia

Rationale:Haemophagocytic lymphohistiocytosis is a rare complication of malaria,which is often *** concerns:A 30-year-old male was admitted to our department for persistent fever,which began after returning from a stay in *** laboratory investigations revealed a pancytopenia and an elevated C-reactive *** smear examination showed Plasmodium falciparum,therefore confirming the diagnosis of *** laboratory tests showed a worsening *** marrow aspiration and biopsy revealed images of ***:The diagnosis of haemophagocytic lymphohistiocytosis complicating malaria infection was ***:The patient was treated with *** immunosuppressant treatment was delivered to the *** received antipyretic and antimalarial treatment *** and lessons:We report a case of haemophagocytic lymphohistiocytosis trigged by malaria infection and we review all reported cases secondary to Plasmodium falciparum malaria by searching PubMed publications till October *** lymphohistiocytos secondary to malaria should be suspected even in non-severe cases of malaria.

关键词： Diagnosis Haemophagocytic lymphohistiocytosis Malaria Plasmodium falciparum

Impact of the professional exposure on the spermatic parameters and the results of ICSI at Tunisian unfertile couples

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Advances in Reproductive Sciences 2014年第1期2卷 24-31页

作者： Habib ben ali Monia Zaouali Fatma Atig Iheb Bougmiza Souhir Mehri Houda ben Frej Faten Tabka ali Saad mounir Ajina Laboratoire de Cytogénétique Génétique Moléculaire et Biologie de la Reproduction Humaines CHU Farhat Hached de Sousse Sousse Tunisie Laboratoire de Physiologie et des Explorations Fonctionnelles Faculté de Médecine de Sousse Sousse Tunisie Service de Médecine Communautaire Faculté de Médecine de Sousse Sousse Tunisie Service de Médecine de Travail CHU Farhat Hached de Sousse Sousse Tunisie Unité de Médecine de la Reproduction CHU Farhat Hached de Sousse Sousse Tunisie

Introduction: The association of the professional exposure to the decline of the male reproductive function had been the subject of several studies but without arriving to one envisagement undeniable of its existence. The ICSI, technique of reference in severe male infertilities, is visavis unexplained failures whose environmental and professional exposure could be about its cause partly. In this work, we tried to highlight an association between certain factors of professional exposure and the deterioration of the spermatic parameters, then to evaluate the effect of such factors on the result of the ICSI. Materials and Methods: It is about a study of the exposed type—no exposed, realized through collaboration between the Unit of Medicine of the Reproduction and the Service of Medicine of Work of the CHU Farhat Hached of Sousse. This study was based on a questionnaire of which part of the items was collected medical files of the patients raising the general characteristics, the profile of exposure as well as the spermatic parameters and the biological assessment of ICSI. Results: Significant associations between the deterioration of sperm and the professional risk factors such as the exposure to the formaldehydes (p = 0.01) and heat (p = 0.001) were noted. The exposure to formaldehydes was associated at the higher risk of oli gozoospermy, of asthenozoospermy with a significant reduction in mobility (p = 0.05), whereas the talks with cement had a risk higher of teratozoospermy. In addition an exposure to heat was associated at the high risk of oligoteratozoospermy. The total rate of pregnancies after ICSI among 220 patients of our series was of 14.47%. The ICSI balance showed a reduction in total fertility rate (76.6% exposed vs 75.3% no exposed). Conclusion: In spite of the limited number of the patients, this study had shown a correlation between certain professional exposures of origin and the deterioration of sperm. They are preliminary results which encourage co

关键词： Male Sterility Professional Exposure Spermogramm ICSI