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SATB2:A versatile transcriptional regulator of craniofacial and skeleton development,neurogenesis and tumorigenesis,and its applications in regenerative medicine

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Genes & Diseases 2022年第1期9卷 95-107,I0005页

作者： Xia Huang Qiuman Chen Wenping Luo Mikhail Pakvasa Yuxin Zhang Liwen Zheng Shuang Li Zhuohui Yang Huan Zeng Fang Liang Fugui Zhang Daniel A.Hu Kevin H.Qin Eric J.Wang David S.Qin Russell R.Reid Tong-Chuan He Aravind Athiviraham mostafa el dafrawy Hongmei Zhang Stomatological Hospital of Chongqing Medical University Chongqing 401147PR China Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences Chongqing 401147PR China Molecular Oncology Laboratory Department of Orthopaedic Surgery and Rehabilitation MedicineThe University of Chicago Medical CenterChicagoIL 60637USA The Pritzker School of Medicine The University of Chicago Medical CenterChicagoIL 60637USA Department of Surgery The University of Chicago Medical CenterChicagoIL 60637USA Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education Chongqing 401147PR China

SATB2(special AT-rich sequence-binding protein 2)is a member of the special AT-rich binding protein *** a transcription regulator,SATB2 mainly integrates higher-order chromatin ***2 expression appears to be tissue-and stage-specific,and is governed by several cellular signaling molecules and *** in branchial arches and osteoblast-lineage cells,SATB2 plays a significant role in craniofacial pattern and skeleton *** addition to regulating osteogenic differentiation,SATB2 also displays versatile functions in neural development and cancer *** an osteoinductive factor,SATB2 holds great promise in improving bone regeneration toward bone defect *** this review,we have summarized our current understanding of the physiological and pathological functions of SATB2 in craniofacial and skeleton development,neurogenesis,tumorigenesis and regenerative medicine.

关键词： Bone regeneration Development Neurogenesis SATB2 Tumorigenesis

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Development of a simplified and inexpensive RNA depletion method for plasmid DNA purification using size selection magnetic beads(SSMBs)

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Genes & Diseases 2021年第3期8卷 298-306页

作者： Xi Wang Ling Zhao Xiaoxing Wu Huaxiu Luo Di Wu Meng Zhan Jing Zhang Mikhail Pakvasa William Wagstaff Fang He Yukun Mao Yongtao Zhang Changchun Niu Meng Wu Xia Zhao Hao Wang Linjuan Huang Deyao Shi Qing Liu Na Ni Kai Fu Kelly Hynes Jason Strelzow mostafa el dafrawy Tong-Chuan He Hongbo Qi Zongyue Zeng Ministry of Education Key Laboratory of Diagnostic Medicine School of Laboratory and Diagnostic MedicineChongqing Medical UniversityChongqing400016PR China Molecular Oncology Laboratory Department of Orthopaedic Surgery and Rehabilitation MedicineThe University of Chicago Medical CenterChicagoIL60637USA Departments of Gastrointestinal Surgery Obstetrics and Gynecologyand NephrologyThe First Affiliated Hospital of Chongqing Medical UniversityChongqing400016PR China Department of Burn and Plastic Surgery West China Hospital of Sichuan UniversityChengduSichuan Province610041PR China Department of Orthopaedic Surgery The First Affiliated HospitalGuangzhou University of Chinese MedicineGuangzhouGuangdong Province510405PR China Departments of Orthopaedic Surgery and Neurosurgery The Affiliated Zhongnan Hospital of Wuhan UniversityWuhanHubei Province430072PR China Department of Orthopaedic Surgery The Affiliated Hospital of Qingdao UniversityQingdaoShandong Province266061PR China Department of Laboratory Diagnostic Medicine Chongqing General HospitalChongqing400021PR China Institute of Boneand Joint Research The departments of Orthopaedic Surgery and Obstetrics and GynecologyThe First and Second Hospitals of Lanzhou UniversityLanzhouGansuProvince730030PR China Department of Orthopaedics Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei Province430022PR China Department of Spine Surgery Second Xiangya HospitalCentral South UniversityChangshaHunan Province410011PR China

Plasmid DNA(pDNA)isolation from bacterial cells is one of the most common and critical steps in molecular cloning and biomedical *** all pDNA purification in-volves disruption of bacteria,removal of membrane lipids,proteins and genomic DNA,purifi-cation of pDNA from bulk lysate,and concentration of pDNA for downstream *** many liquid-phase and solid-phase pDNA purification methods are used,the final pDNA preparations are usually contaminated with varied degrees of host RNA,which cannot be completely digested by RNase *** develop a simple,cost-effective,and yet effective method for RNA depletion,we investigated whether commercially available size selection magnetic beads(SSMBs),such as Mag-Bind®TotalPure NGS Kit(or Mag-Bind),can completely deplete bacterial RNA in pDNA *** this proof-of-principle study,we demonstrated that,compared with RNase A digestion and two commercial plasmid affinity purification kits,the SSMB method was highly efficient in depleting contaminating RNA from pDNA *** transfection and bacterial colony formation assays revealed that pDNA purified from SSMB method had superior quality and integrity to pDNA samples cleaned up by RNase A digestion and/or commercial plasmid purification *** further demonstrated that the SSMB method completely depleted contaminating RNA in large-scale pDNA ***,the Mag-bind-based SSMB method costs only 5-10%of most commercial plasmid purification kits on a per sample ***,the reported SSMB method can be a valuable and inexpensive tool for the removal of bacterial RNA for routine pDNA preparations.

关键词： DNA transfection DNA vaccination Gene delivery Plasmid DNA purification RNA depletion Size selection magnetic beads

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Notch signaling:Its essential roles in bone and craniofacial development

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Genes & Diseases 2021年第1期8卷 8-24页

作者： Mikhail Pakvasa Pranav Haravu Michael Boachie-Mensah Alonzo Jones elam Coalson Junyi Liao Zongyue Zeng Di Wu Kevin Qin Xiaoxing Wu Huaxiu Luo Jing Zhang Meng Zhang Fang He Yukun Mao Yongtao Zhang Changchun Niu Meng Wu Xia Zhao Hao Wang Linjuan Huang Deyao Shi Qing Liu Na Ni Kai Fu Michael J.Lee Jennifer Moriatis Wolf Aravind Athiviraham Sherwin S.Ho Tong-Chuan He Kelly Hynes Jason Strelzow mostafa el dafrawy Russell R.Reid Molecular Oncology Laboratory Department of Orthopaedic Surgery and Rehabilitation MedicineThe University of Chicago Medical CenterChicagoIL 60637USA Pritzker School of Medicine University of ChicagoChicagoIL 60637USA Section of Plastic and Reconstructive Surgery Department of SurgeryThe University of Chicago Medical CenterChicagoIL 60637USA Departments of Orthopaedic Surgery Gastrointestinal SurgeryObstetrics and Gynecologyand NephrologyThe First Affiliated Hospital of Chongqing Medical UniversityChongqing400016PR China Ministry of Education Key Laboratory of Diagnostic Medicine and School of Laboratory and Diagnostic MedicineChongqing Medical UniversityChongqing400016PR China Department of Burn and Plastic Surgery West China Hospital of Sichuan UniversityChengduSichuan610041PR China Department of Orthopaedic Surgery The First Affiliated HospitalGuangzhou University of Chinese MedicineGuangzhouGuangdong510405PR China Departments of Orthopaedic Surgery and Neurosurgery The Affiliated Zhongnan Hospital of Wuhan UniversityWuhanHubei430072PR China Department of Orthopaedic Surgery The Affiliated Hospital of Qingdao UniversityQingdaoShandong266061PR China Department of Laboratory Diagnostic Medicine Chongqing General HospitalChongqing400021PR China Institute of Bone and Joint Research and the Department of Orthopaedic SurgeryThe Second Hospitals of Lanzhou UniversityGansuLanzhou730030PR China Department of Orthopaedics Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei430072PR China Department of Spine Surgery Second Xiangya HospitalCentral South UniversityChangshaHunan410011PR China

Notch is a cellecell signaling pathway that is involved in a host of activities including development,oncogenesis,skeletal homeostasis,and much *** specifically,recent research has demonstrated the importance of Notch signaling in osteogenic differentiation,bone healing,and in the development of the *** craniofacial skeleton is complex and understanding its development has remained an important focus in *** this review we briefly summarize what recent research has revealed about Notch signaling and the current understanding of how the skeleton,skull,and face *** then discuss the crucial role that Notch plays in both craniofacial development and the skeletal system,and what importance it may play in the future.

关键词： Alagille syndrome Bone Craniofacial development Craniosynostosis Notch Oncogenesis Osteogenesis Spondylocostal dysosotosis

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