检索结果-南通市图书馆

Prevalence of diabetic retinopathy in Iran： a systematic review and Meta-analysis

International Journal of Ophthalmology(English edition) 2017年第5期10卷 782-789页

作者： Saman Maroufizadeh Amir Almasi-Hashiani mostafa Hosseini Mahdi Sepidarkish Reza Omani Samani Department of Epidemiology and Reproductive Health Reproductive Epidemiology Research Center Royan Institute for Reproductive Biomedicine ACECR Tehran 16635-148Iran Department of Epidemiology and Biostatistics School of Public Health Tehran University of Medical Sciences Tehran 14155-6446 Iran

AIM：To estimate the overall prevalence of diabetic retinopathy（DR） in Iran by a systematic review and ***：We conducted a search of all published literature on diabetic patients for the prevalence of DR using Web of Sciences, Pub med, Scopus, Google Scholar, and national electronic databases SID, Magiran, and Ira.medex from their inception until September 2016 with standard keywords. Pooled estimates of the DR prevalence and the corresponding 95% confidence intervals（CI） were calculated using random effects ***：Thirty-one studies involving 23 729 patients with type Ⅰ and Ⅱ diabetes were included. The publication bias assumption for prevalence of DR was rejected by Begg and Egger tests（P=0.825, P=0.057, respectively）. The results of Cochran test and I^2 statistics showed considerable heterogeneity for prevalence of DR（Q=1278.21, d.f.=30, P〈0.001 and I^2=97.7%）. The prevalence of DR, non-proliferative diabetic retinopathy（NPDR） and proliferative diabetic retinopathy（PDR） in Iranian diabetic patients were 41.9%（95% CI：35.6-48.2）, 32.2%（95% CI：28.7-35.8）, and 13.2%（95% CI：8.3-18.1）, respectively. CONCLUSION：The prevalence of DR in Iran appears a little high. NPDR was more common. This study highlights the necessity for DR screening and management in diabetic patients in Iran.

关键词： diabetic retinopathy Iran prevalence Meta-analysis systematic review

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Antibody to El peptide of hepatitis C virus genotype 4 inhibits virus binding and entry to HepG2 cells in vitro

引用

World Journal of Gastroenterology 2006年第16期12卷 2530-2535页

作者： mostafa K EL-Awady Ashraf A Tabll Khaled Atef Samar S Yousef Moataza H Omran Yasmin EI-Abd Noha G Bader-Eldin Ahmad M Salem Samir F Zohny Wael T EI-Garf Department of Biomedical Technology National Research CenterCairoEgypt Department of Biochemistry Faculty of ScienceAin Shams UniversityCairoEgypt

AIM： To analyze the neutralizing activity of antibodies against E1 region of hepatitis C virus （HCV）. Specific polydonal antibody was raised via immunization of New Zealand rabbits with a synthetic peptide that had been derived from the E1 region of HCV and was shown to be highly conserved among HCV published genotypes. METHODS： Hyper-immune HCV E1 antibodies were incubated over night at 4 ℃ with serum samples positive for HCV RNA, with viral loads ranging from 615 to 3.2 million IU/mL. Treated sera were incubated with HepG2 cells for 90 min. Blocking of viral binding and entry into cells by anti E1 antibody were tested by means of RTPCR and flow cytometry. RESULTS： Direct immunostaining using FITC conjugated E1 antibody followed by Flow cytometric analysis showed reduced mean fluorescence intensity in samples pre-incubated with E1 antibody compared with untreated samples. Furthermore, 13 out of 18 positive sera （72%） showed complete inhibition of infectivity as detected by RT-PCR. CONCLUSION： In house produced E1 antibody, blocks binding and entry of HCV virion infection to target cells suggesting the involvement of this epitope in virus binding and entry. Isolation of these antibodies that block virus attachment to human cells are useful as therapeutic reagents.

关键词： Flow cytometry Hepatitis C virus E1 envelope Therapeutic antibodies Direct immuno-fluorescence HepG2 cells

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

HepG2 cells support viral replication and gene expression of hepatitis C virus genotype 4 in vitro

引用

World Journal of Gastroenterology 2006年第30期12卷 4836-4842页

作者： mostafa K El-Awady Ashraf A Tabll Yasmine S El-Abd Mahmoud M Bahgat Hussein A Shoeb Samar S Youssef Noha G Bader El Din El-Rashdy M Redwan Maha El-Demellawy Moataza H Omran Wael T El-Garf Said A Goueli Department of Biomedical Technology National Research Center Tahrir Street PO 12622 Dokki Cairo Egypt Department of medicinal Chemistry Na- tional Research Center Tahrir Street PO 12622 Dokki Cairo Egypt Department of Microbiology and Immunology Faculty of Pharmacy Cairo University Cairo Egypt Genetic Engineering and Biotechnology Research Institute New Borg El-Arab City Alexandria Egypt Department of Pathology School of medicine University of Wisconsin Madison WI United States

AIM： TO establish a cell culture system with longterm replication of hepatitis C virus （HCV） genome and expression of viral antigens in vitro. METHODS： HepG2 cell line was tested for its susceptibility to HCV by incubation with a serum from a patient with chronic hepatitis C. Cells and supernatant were harvested at various time points during the culture. Culture supernatant was tested for its ability to infect na＇ive cells. The presence of minus （antisense） RNA strand, and the detection of core and E1 antigens in cells were examined by RT-PCR and immunological techniques （flow cytometry and Western blot） respectively. RESULTS： The intracellular HCV RNA was first detected on d 3 after infection and then could be consistently detected in both cells and supernatant over a period of at least three months. The fresh cells could be infected with supernatant from cultured infected cells. Flow cytometric analysis showed surface and intracellular HCV antigen expression using in house made polyclonal antibodies （anti-core, and anti-E1）. Western blot analysis showed the expression of a cluster of immunogenic peptides at molecular weights extended between 31 and 45 kDa in an one month old culture of infected cells whereas this cluster was undetectable in uninfected HepG2 cells. CONCLUSION： HepG2 cell line is not only susceptible to HCV infection but also supports its replication in vitro. Expression of HCV structural proteins can be detected in infected HepG2 cells. These cells are also capable of shedding viral particles into culture media which in turn become infectious to uninfected cells.

关键词： Hepatitis C virus In vitro propagation Genomic replication Gene expression HepG2 cells

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：