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CRISPR/Cas9-mediated genome editing reveals 12 testis-enriched genes dispensable for male fertility in mice

Asian Journal of Andrology 2022年第3期24卷 266-272页

作者： Yuki Oyama Haruhiko Miyata Keisuke Shimada Yoshitaka Fujihara Keizo Tokuhiro Thomas X garcia Martin M Matzuk Masahito Ikawa Graduate School of Pharmaceutical Sciences Osaka UniversitySuitaOsaka 565-0871Japan Department of Experimental Genome Research Research Institute for Microbial DiseasesOsaka UniversitySuitaOsaka 565-0871Japan Department of Bioscience and Genetics National Cerebral and Cardiovascular CenterSuitaOsaka 564-8565Japan Department of Genome Editing Institute of Biomedical ScienceKansai Medical UniversityHirakataOsaka 573-1191Japan Center for Drug Discovery Baylor College of MedicineHoustonTX 77030USA Department of Pathology and Immunology Baylor College of MedicineHoustonTX 77030USA Department of Biology and Biotechnology University of Houston-Clear LakeHoustonTX 77058USA The Institute of Medical Science The University of TokyoMinato-kuTokyo 108-8639Japan

Gene expression analyses suggest that more than 1000–2000 genes are expressed predominantly in mouse and human *** functional analyses of hundreds of these genes have been performed,there are still many testis-enriched genes whose functions remain *** gene function using knockout(KO)mice is a powerful tool to discern if the gene of interest is essential for sperm formation,function,and male fertility in *** this study,we generated KO mice for 12 testis-enriched genes,1700057G04Rik,4921539E11Rik,4930558C23Rik,Cby2,Ldhal6b,Rasef,Slc25a2,Slc25a41,Smim8,Smim9,Tmem210,and Tomm20l,using the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9(CRISPR/Cas9)*** designed two gRNAs for each gene to excise almost all the protein-coding regions to ensure that the deletions in these genes result in a null *** tests of KO mice reveal that these 12 genes are not essential for male fertility,at least when individually ablated,and not together with other potentially compensatory paralogous *** results could prevent other laboratories from expending duplicative effort generating KO mice,for which no apparent phenotype exists.

关键词： CRISPR/Cas9 knockout mice male infertility spermatozoa testis

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Diagnosis, treatment, and current concepts in the endoscopic management of gastroenteropancreatic neuroendocrine neoplasms

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World Journal of Gastroenterology 2022年第34期28卷 4943-4958页

作者： Giuseppe Iabichino Milena Di Leo monica Arena Giovanni Giuseppe Rubis Passoni Elisabetta Morandi Francesca Turpini Paolo Viaggi Carmelo Luigiano Luca De Luca Digestive Endoscopy Unit ASST Santi Paolo e CarloMilano 20144Italy Gastroenterology Section Grande Ospedale Metropolitano“Bianchi-Melacrino-Morelli”Reggio Calabria 89124Italy

Gastroenteropancreatic neuroendocrine neoplasms(GEP-NENs)are rare tumors derived from the neuroendocrine cell system,which that have increased in incidence and prevalence in recent *** improvements in radiological and metabolic imaging,endoscopy still plays a pivotal role in the number of *** detection,characterization,and staging are essential in management and treatment *** and lower gastrointestinal(GI)endoscopy is essential for correct localization of the primary tumor site of GI *** ultrasonography(EUS)has an important role in the imaging and tissue acquisition of pancreatic NENs and locoregional staging of GI neuroendocrine *** staging and histological diagnosis have important prognostic *** operating techniques allow the removal of small GI NENs in the early stage of mucosal or submucosal invasion of the intestinal *** EUS-guided techniques may help the surgeon locate small and deep tumors,thus avoiding formal pancreatic resections in favor of parenchymal-sparing ***,locoregional ablative treatments have been proposed in recent studies with promising results in selected patients.

关键词： Neuroendocrine neoplasms Gastrointestinal endoscopy Endoscopic resection Endoscopic ultrasound Ablative technique Tissue acquisition

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A glance at three-dimensional Alexandrov spaces

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Frontiers of Mathematics in China 2016年第5期11卷 1189-1206页

作者： Fernando GALAZ-garcia Institut fur Algebra und Geometrie Karlsruher Institut fiir Technologie (KIT) Karlsruhe Germany

We discuss the topology and geometry of closed Alexandrov spaces of dimension three.

关键词： Alexandrov space group action 3-manifold

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Preoperative and postoperative risk factors for periprosthetic joint infection in primary total hip arthroplasty:A 1-year experience

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World Journal of Orthopedics 2022年第10期13卷 903-910页

作者： Giuseppe Ferdinando Tella Cesare Donadono Francesco Castagnini Barbara Bordini monica Cosentino Michele Di Liddo Francesco Traina Ortopedia-Traumatologia e Chirurgia protesica e dei reimpianti d'anca e di ginocchio IRCCS Istituto Ortopedico RizzoliBologna 40125Italy Laboratorio di Tecnologia Medica IRCCS Istituto Ortopedico RizzoliBologna 40125Italy

BACKGROUND Periprosthetic joint infection(PJI)in primary total hip replacement(THR)is one of the most important threats in orthopedic surgery,so one important surgeon’s target is to avoid or early diagnose a *** the incidence of PJI is very low(0.69%)in our department,with an average follow-up of 595 d,this infection poses a serious threat due to the difficulties of treatment and the lower functional outcomes after *** To study the incidence of PJI in all operations occurring in the year 2016 in our department to look for predictive signs of potential *** We counted 583 THR for 578 patients and observed only 4 cases of infection(0.69%)with a mean follow-up of 596 d(min 30,max 1451).We reviewed all medical records to collect the data:duration and time of the surgery,presence,type and duration of the antibiotic therapy,preoperative diagnosis,blood values before and after surgery,transfusions,presence of preoperative drugs(in particularly anticoagulants and antiaggregant,corticosteroids and immunosuppressants),presence of some comorbidities(high body mass index,blood hypertension,chronic obstructive pulmonary disease,cardiac ischemia,diabetes,rheumatological conditions,previous local infections).RESULTS No preoperative,intraoperative,or postoperative analysis showed a higher incidence of *** did not find any class with evident major odds of *** our study,we did not find any border value to predict PJI and all patients had similar values in both groups(non-PJI and PJI).Only some categories,such as female patients,showed more frequency of PJI,but this difference related to sex was not statistically *** We did not find any category with a higher risk of PJI in THR,probably due to the lack of few cases of infection.

关键词： Primary total hip replacement Periprosthetic joint infection Preoperative risk factors Postoperative risk factors Preoperative and postoperative blood value Total hip arthroplasty

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Overview of the anterolateral complex of the knee

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World Journal of Clinical Cases 2022年第24期10卷 8474-8481页

作者： Ignacio garcia-Mansilla Juan Pablo Zicaro Ezequiel Fernando Martinez Juan Astoul Carlos Yacuzzi MatiasCosta-Paz Knee Surgery Hospital Italiano de Buenos AiresBuenos Aires 1199Argentina

In the last few years,much more information on the anterolateral complex of the knee has become *** has now been demonstrated how it works in conjunction with the anterior cruciate ligament(ACL)controlling anterolateral rotatory *** studies have shown that the anterolateral complex(ALC)has a role as a secondary stabilizer to the ACL in opposing anterior tibial translation and internal tibial *** is of utmost importance that surgeons comprehend the intricate anatomy of the entire anterolateral aspect of the *** most studies have only focused on the anterolateral ligament(ALL),the ALC of the knee consists of a functional unit formed by the layers of the iliotibial band combined with the anterolateral joint *** interest has also been given to imaging evaluation using magnetic resonance and several studies have targeted the evaluation of the ALC in the setting of ACL *** are inconsistent with a lack of association between magnetic resonance imaging evidence of injury and clinical *** ACL reconstruction may not always reestablish knee rotatory stability in patients with associated ALC *** such cases,additional procedures,such as anterolateral reconstruction or lateral tenodesis,may be *** are several techniques available for ALL *** options include the iliotibial band,gracilis or semitendinosus tendon autograft,or allograft.

关键词： Anterolateral complex Knee instability Anterolateral ligament Anterior cruciate ligament reconstruction

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Fibonacci Sequences and the Multiperiodicity of the Variable Star UW Herculis

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Chinese Journal of Astronomy and Astrophysics 2003年第3期3卷 235-240页

作者： Juan garcia Escudero Departamento de Fisica Universidad de Oviedo 33007 Oviedo Spain

We present an application of the methods recently developed for the study of quasicrystal structures to the analysis of multiperiodicity of semiregular variables. A light curve analysis of UW Her shows frequencies that can be included within the general scheme characterizing the Fourier spectra of Fibonacci quasiperiodic sequences. The analysed data come from the BAA Variable Star Section computerised archive.

关键词： stars: variables

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赞比亚与中国遗传资源获取与惠益分享制度比较研究

赞比亚与中国遗传资源获取与惠益分享制度比较研究

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作者： monica Kayafa（苏晓）浙江农林大学

学位级别：硕士

根据《生物多样性公约》的规定,各国对其生物资源(包括遗传资源)享有所有权,基于遗传资源获取的便利性与利用的可持续性原则,制定相应法律以规范遗传资源的取得与利用。为此,各国在立足于本国国情基础上,纷纷展开了有关遗传资源享有与... 详细信息

根据《生物多样性公约》的规定,各国对其生物资源(包括遗传资源)享有所有权,基于遗传资源获取的便利性与利用的可持续性原则,制定相应法律以规范遗传资源的取得与利用。为此,各国在立足于本国国情基础上,纷纷展开了有关遗传资源享有与使用的立法与理论研究。中国和赞比亚作为遗传资源较为丰富的两个发展中国家也不例外。基于国情、立法体例的不同,两国的有关遗传和获益分享制度(简称ABS制度)从形式上虽有共同之处,但实质内容却呈现截然不同的特点。鉴于此,本文将以中国和赞比亚两国的遗传资源惠益与分享法律制度及司法实践为研究对象,采用比较研究方法,通过分析比较中国与赞比亚两国在ABS理论及实践中的差异性,具体包括两国关于遗传资源使用管理制度、遗传资源享有与使用主体制度、农民和植物育种者的权益保护制度,以及当地人和土著社区的惠益分享制度,总结得出,ABS制度在两国实施过程中因“生物剽窃”、误用知识产权等因素所造成的困境和挑战,并在此基础上,提出两国可以采用取长补短之法,通过相互借鉴各自立法经验,以完善两国遗传资源使用制度的不足,希望不仅为能我自己祖国的学者们,也为我的第二故乡-中国的学者们提供一些可以借鉴的经验。

关键词：获取和惠益分享遗传资源传统知识生物剽窃知识产权

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The RNA binding proteins TIA1 and TIAL1 promote Mcl1 mRNA translation to protect germinal center responses from apoptosis

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Cellular & Molecular Immunology 2023年第9期20卷 1063-1076页

作者： Ines COsma-garcia Mailys Mouysset Dunja Capitan-Sobrino Yann Aubert Martin Turner Manuel D.Diaz-Muñoz Institute for Infectious and Inflammatory Diseases(INFINITy) Inserm UMR1291CNRS UMR5051University Paul SabatierCHU PurpanToulouse 31024France Immunology Program The Babraham InstituteCambridgeUK

Germinal centers(GCs)are essential for the establishment of long-lasting antibody *** B cells rely on post-transcriptional RNA mechanisms to translate activation-associated transcriptional programs into functional changes in the cell ***,the critical proteins driving these key mechanisms are still ***,we show that the RNA binding proteins TIA1 and TIAL1 are required for the generation of long-lasting GC ***1-and TIAL1-deficient GC B cells fail to undergo antigen-mediated positive selection,expansion and differentiation into B-cell clones producing high-affinity ***,TIA1 and TIAL1 control the transcriptional identity of dark-and light-zone GC B cells and enable timely expression of the prosurvival molecule ***,we demonstrate here that TIA1 and TIAL1 are key players in the post-transcriptional program that selects high-affinity antigen-specific GC B cells.

关键词： Adaptive immunity Germinal centers Post-transcriptional gene regulation RNA binding proteins Cell identity Apoptosis/10.1038/s41423-023-01063-4

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Sperm subpopulations in avian species： a comparative study between the rooster （Gallus domesticus） and Guinea fowl （Numida meleagris）

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Asian Journal of Andrology 2016年第6期18卷 889-894页

作者： Manuel garcia-Herreros National Secretariat of Higher Education Science Technology and Innovation (SENESCYT) Quito Ecuador

The main aims of this research were to study possible differences in objective morphometric sperm characteristics, establish normative sperm morphometry standards, and evaluate the presumed different subpopulation distribution of avian spermatozoa from the rooster （Gallus domesticus） and Guinea fowl （Numida meleagris） as model avian species. Seventy-two ejaculates （36 per species studied） were obtained manually, following a training period involving gently combined dorso-abdominal and *** massage of the birds. Ejaculates were processed for volume, sperm concentration, viability, motility, and morphology. Moreover, samples were submitted for sperm morphometric assessment using objective Computer-Assisted Semen Analysis for Morphometry （CASA-Morph） methods, with sperm morphometric descriptors evaluated by Principal Component Analysis （PCA） and multivariate clustering analyses. There were several differences observed between the avian species in values obtained for ejaculate volume and sperm concentration （P 〈 0.001）. Irrespective of species, PCA revealed two Principal Components （PCs） explaining more than 80% of the variance. In addition, the number of subpopulations differed with species （three and five subpopulations for rooster and Guinea fowl, respectively）. Moreover, the distribution of the sperm subpopulations was found to be structurally different between species. In conclusion, our findings from using CASA-Morph methods indicate pronounced sperm morphometric variation between these two avian species. Because of the strong differences observed in morphometric parameter values and their subpopulation distribution, these results suggest that application of objective analytical methods such as CASA-Morph could substantially improve the reliability of comparative studies and help establish valid normative sperm morphological values for avian species.

关键词： Gallus domesticus Numida meleaEris principal component analysis sperm morphometry sperm subpopulations

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Nutritional and antinutritional compounds in leaves of quinoa

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Food Bioscience 2022年第1期45卷 166-173页

作者： Vasile Stoleru Sven-Erik Jacobsen Maricel Vitanescu Gerard Jitareanu monica Butnariu Neculai Munteanu Teodor Stan Gabriel Ciprian Teliban Alexandru Cojocaru Gabriela Mihalache Horticulture Department “Ion Ionescu de la Brad”Iasi University of Life Sciences3 M.Sadoveanu700440IasiRomania Quinoa Quality ApS CVR 40610588Teglværksvej 10DK-4420Denmark Research Institute for Agriculture and Environment 14 M.Sadoveanu700789IasiRomania Chemistry&Biochemistry Department “King Michael I of Romania”Banat’s University of Agricultural Sciences and Veterinary Medicine from TimisoaraCalea Aradului 119300645TimisRomania Integrated Center of Environmental Science Studies in the North Eastern Region(CERNESIM) “Alexandru Ioan Cuza”University of Iasi11 Carol I700506IasiRomania

In the context of climate change,especially for the temperate continental climate,new potential crop species are emerging,originating from the *** of them is quinoa(Chenopodium quinoa Willd.)with multiple benefits for seed and leaf *** is native to South America,grown mainly for seeds,with a high ecological *** is known about the potential of using quinoa plants as a leafy vegetable for food *** this study,the nutritional and antinutritional content of quinoa leaves was evaluated in three cultivars(Titicaca,Puno,Vikinga),considering different densities and times of *** cultivar had a higher total content of carbohydrates,lipids,proteins and dietary fibers,and lower mineral contents in *** levels of antinutrient compounds were found in Vikinga *** the time of the crop establishment,the highest content of primary metabolic compounds(carbohydrates,lipids,proteins,dietary fibers)was achieved by April 17,the sowing *** densities of 7.7,3.2,and 1.6 ***/ha did not significantly influence the content of compounds with antinutritive role,such as oxalates,saponins or trypsin *** content of mineral elements such as:Fe,Zn,Na and K were significantly influenced by the cultivar,compared to Mg and Ca whose values were insignificant regardless of the treatment.

关键词： Chenopodium quinoa Willd. Cultivars Sowing time Plant density Nutritive and antinutritive content

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