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Agricultural Sciences in China 2009年第11期8卷 1351-1359页

作者： ZHAO Ying LIU na NIU Jian-xin Horticultural department Agricultural College Shihezi University Shihezi 832003 P.R. China

To understand the distribution of Apple stem pitting virus （ASPV） in tissues of pear tree and provide directly theorical and technical support for shoot tips detoxication, leaves and shoot tips of Korla pear were used as the materials, cdna probe for ASPV was synthesized through RT-PCR reaction system using the unradioactive digoxigenin-labeled probe, and the specificity and sensitivity of probe were verified by blot hybridization method. Paraffin slice for in situ PCR and in situ hybridization was made and the location and distribution of ASPV Rna were detected in paraffin slices using in situ reverse transcription polymerase chain reaction, and the important factors which influenced the experiment results were optimized. ASPV mainly distributed in palisade tissue of mesophyll cells, external cortex of the tip, and the corresponding newborn vascular bundles. 20 min was the suitable digestive time for proteinase K. For the better amplication, RT reaction system should be above 0.2 U μL^-1 and 0.4 mmol L^-1 for Rnasin and dNTPs respectively, 0.1-1.3 U μ L^-1 SuperScript Ⅱ, 0.6- 0.8 μmol L^-1 primer concentration, and above 0.5 U 100 μL^-1 LA Taq dna polymerase. The suitable annealing temperature in PCR reaction was 60℃ with 35 cycles. The apical meristem of 0.25 mm was the region of virus-free.

关键词： Korla pear Apple stem pitting virus cdna probe digoxigenin in situ PCR

Morbihan disease misdiagnosed as senile blepharoptosis and successfully treated with short-term minocycline and ketotifen: A case report

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World Journal of Clinical Cases 2023年第19期11卷 4692-4697页

作者： Jun na Yan Wu department of dermatology Peking University First HospitalBeijing 100034China

BACKGROUNd Morbihan disease is a rare skin condition with diagnostic and therapeutic *** nonpitting erythematous edema is usually considered to be a characteristic manifestation and diagnostic clue for the Morbihan *** of Morbihan disease remains a dilemma due to its long course,poor response,and high recurrence *** SUMMARY We report the case of a 69-year-old man with Morbihan *** patient presented ptosis as the first and main *** was no obvious edema or other skin *** patient was misdiagnosed with senile blepharoptosis based on eyelid performance and no treatment was administered to *** continuous progressive asthenia of eye-opening and ptosis for more than one year,a skin biopsy was *** analysis showed edema in the dermis,lymphatic hyperplasia and dilatation,and perivascular lymphocytic *** obvious increase in toluidine blue-stained mast cells was *** patient was finally diagnosed with Morbihan *** and ketotifen were prescribed based on the increase of mast cells in skin tissue *** patient experienced rapid relief seven days later and complete remission after 40 d from the commencement of the *** Ptosis without obvious swelling could be the only or main clinical manifestation of Morbihan disease in rare *** increase of mast cells was an important therapeutic clue to the rapid and remarkable efficiency of the combination therapy of minocycline and antihistamine.

关键词： Morbihan disease Ptosis Minocycline Mast cells Treatment Case report

Molecular mechanism of flowering time regulation in Brassica rapa:similarities and differences with Arabidopsis

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Horticultural Plant Journal 2024年第3期10卷 615-628页

作者： na Li Rui Yang Shuxing Shen Jianjun Zhao State Key Laboratory of North China Crop Improvement and Regulation Key Laboratory of Vegetable Germplasm Innovation and Utilization of HebeiCollaborative Innovation Center of Vegetable Industry in HebeiCollege of HorticultureHebei Agricultural UniversityBaodingHebei 071000China

Properly regulated flowering time is pivotal for successful plant *** floral transition from vegetative growth to reproductive growth is regulated by a complex gene regulatory network that integrates environmental signals and internal conditions to ensure that flowering takes place under favorable *** rapa is a diploid Cruciferae species that includes several varieties that are cultivated as vegetable or oil *** time is one of the most important agricultural traits of *** crops because of its influence on yield and *** transition to flowering in *** is regulated by several environmental and developmental cues,which are perceived by several signaling pathways,including the vernalization pathway,the autonomous pathway,the circadian clock,the thermosensory pathway,and gibberellin(GA)*** signals are integrated to control the expression of floral integrators BrFTs and BrSOC1s to regulate *** this review,we summarized current research advances on the molecular mechanisms that govern flowering time regulation in *** and compare this to what is known in Arabidopsis.

关键词： Flowering time Brassica rapa Vernalization Photoperiod

Reactive Transport Process of Earthquake-induced Hydrochemical Changes in Guanding Thermal Spring,Western Sichuan,China

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Acta Geologica Sinica(English Edition) 2024年第1期98卷 241-249页

作者： na Jin JIANG Xue SHI Zheming CHEN Yanmei Hubei Key Laboratory of Petroleum Geochemistry and Environment(Yangtze University) Wuhan 430100China School of Environmental Studies China University of GeosciencesWuhan 430074China School of Water Resources and Environment China University of GeosciencesBeijing 100083China State Key Laboratory of Water Resource Protection and Utilization in Coal Mining Beijing 100000China

Earthquake-related hydrochemical changes in thermal springs have been widely observed;however,quantitative modeling of the reactive transport process is *** the present study,we apply reactive transport simulation to capture the hydrochemical responses in a thermal spring following the Wenchuan Ms 8.0 and Lushan Ms 7.0 *** first constrain deep reservoir geothermal fluid compositions and temperature by multicomponent geothermometry,and then a reactive geochemical transport model is constructed to reproduce the hydrochemical evolution *** results show that the recharge from the shallow aquifer increases gradually until it reaches a peak because of the permeability enhancement caused by the Lushan earthquake,which may be the mechanism to explain the earthquake-related hydrochemical *** contrast to the postseismic effect of the Wenchuan earthquake,the chemical evolution can be considered as hydrochemical anomalies related to the Lushan *** study proves that the efficient simulation of reactive transport processes is useful for investigating earthquake-related signals in hydrochemical time series.

关键词： earthquake hydrochemical changes reactive geochemical transport model Kangding area

Optimal fusion rule for cooperative spectrum sensing in cognitive radio networks

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The Journal of China Universities of Posts and Telecommunications 2012年第5期19卷 58-65页

作者： WANG Qian YUE dian-wu YAN Qiu-na College of Information Science and Technology Dalian Maritime University Dalian 116026 China national Mobile Communications Research Laboratory of Southeast University Nanjing 210096 China

In this paper, we analyze performance of cooperative spectrum sensing under counting rules when exponential model is utilized to characterize the burst nature of primary user （PU） link. Our objective is to minimize the average error probability （AEP） so that the link utilization in the considered link achieves its maximum. We derive a closed-form expression of AEP as well as the probability of interference （PoI） by classifying cognitive transmission into six events. Then, we consider the minimization of AEP over counting rules under the constraint of interference. As the solution, we develop an efficient algorithm to evaluate the optimal fusion rule. Finally, we verify our analysis in numerical results.

关键词： cooperative spectrum sensing cognitive radio network average error probability countering rule probability of interference,spectrum usage

Water Vapor Retrievals from Near-infrared Channels of the Advanced Medium Resolution Spectral Imager Instrument onboard the Fengyun-3d Satellite

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Advances in Atmospheric Sciences 2021年第8期38卷 1351-1366页

作者： Ling WANG Xiuqing HU na XU Lin CHEN Key Laboratory of Radiometric Calibration and Validation for Environmental Satellites China Meteorological AdministrationBeijing 100081China national Satellite Meteorological Center China Meteorological AdministrationBeijing 100081China

Water vapor plays a key role in weather, climate and environmental research on local and global scales. Knowledge about atmospheric water vapor and its spatiotemporal variability is essential for climate and weather research. Because of the advantage of a unique temporal and spatial resolution, satellite observations provide global or regional water vapor distributions. The advanced Medium Resolution Spectral Imager (MERSI) instrument-that is, MERSI-II-onboard the Fengyun-3d (FY-3d) meteorological satellite, has been one of the major satellite sensors routinely providing precipitable water vapor (PWV) products to the community using near-infrared (NIR) measurements since June 2018. In this paper, the major updates related to the production of the NIR PWV products of MERSI-II are discussed for the first time. In addition, the water vapor retrieval algorithm based on the MERSI-II NIR channels is introduced and derivations are made over clear land areas, clouds, and sun-glint areas over the ocean. Finally, the status and samples of the MERSI-II PWV products are presented. The accuracy of MERSI-II PWV products is validated using ground-based GPS measurements. The results show that the accuracies of the water vapor products based on the updated MERSI-II instrument are significantly improved compared with those of MERSI, because MERSI-II provides a better channel setting and new calibration method. The root- mean-square error and relative bias of MERSI-II PWV products are typically 1.8-5.5 mm and −3.0% to −14.3%, respectively, and thus comparable with those of other global remote sensing products of the same type.

关键词： precipitable water vapor FY-3d MERSI GPS

Update on Mayer—Rokitansky—Küster—Hauser syndrome

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Frontiers of Medicine 2022年第6期16卷 859-872页

作者： na Chen Shuang Song Xinmiao Bao Lan Zhu national Clinical Research Center for Obstetric and Gynecologic diseases Department of Obstetrics and GynecologyState Key Laboratory of Complex Severe and Rare DiseasesPeking Union Medical College HospitalPeking Union Medical College and Chinese Academy of Medical SciencesBeijing100730China Peking Union Medical College M.D.ProgramBeijing100730China

This review presents an update of Mayer—Rokitansky—Küster—Hauser(MRKH)syndrome on its etiologic,clinical,diagnostic,psychological,therapeutic,and reproductive *** etiology of MRKH syndrome remains unclear due to its intrinsic *** and genetic causes that may interact during the embryonic development have been proposed with no definitive etiopathogenesis *** proportion of concomitant extragenital malformations varies in different studies,and the discrepancies may be explained by ethnic *** addition to physical examination and pelvic ultrasound,the performance of pelvic magnetic resonance imaging is crucial in detecting the presence of rudimentary uterine *** syndrome has long-lasting psychological effects on patients,resulting in low esteem,poor coping strategies,depression,and anxiety *** psychological counseling and peer support to diagnosed patients is *** and timely psychological intervention could significantly improve a patient’s *** nonsurgical and surgical methods have been suggested for treatment of MRKH *** to the high success rate and minimal risk of complications,vaginal dilation has been proven to be the first-line *** is the second-line option for patients experiencing dilation *** transplantation and gestational surrogacy are options for women with MRKH syndrome to achieve biological motherhood.

关键词： MRKH(Mayer-Rokitansky-Küster-Hauser)syndrome etiology clinical characteristic diagnosis treatment psychological effect

Molecular mechanisms of stress resistance in sorghum: Implications for crop improvement strategies

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Journal of Integrative Agriculture 2024年第3期23卷 741-768页

作者： Hongxiang Zheng Yingying dang Xianmin diao na Sui Shandong Provincial Key Laboratory of Plant Stress/College of life Sciences Shandong Normal UniversityJinan 250014China Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China

Abiotic stresses, such as drought, salt, extreme temperatures, and heavy metal pollution, are the main environmental factors that limit crop growth and yield. Sorghum, a C4 grass plant with high photosynthetic efficiency, can grow in adverse environmental conditions due to its excellent stress resistance characteristics. Therefore, unraveling the stress-resistance mechanism of sorghum could provide a theoretical basis for developing and cultivating various stress-resistant crops. This understanding could also help to create a conducive environment for using marginal soil in agriculture and ensuring food security. In this review, we discuss the adaptation mechanisms of sorghum under drought, salinity, temperature, and soil heavy metal stresses, the specific response to stress, the screening of sorghum-resistant germplasm, and the identification and functional analysis of the relevant genes and quantitative trait loci(QTL). In addition, we discuss the application potential of different stress-tolerant sorghum germplasms reported to date and emphasize the feasibility and potential use in developing and promoting highly stress-tolerant sorghum in marginal soil.

关键词： abiotic stress C4 plants QTL sorghum stress resistance yield stability

Lanthanide complexes-functionalized ordered mesoporous TiO_2:Multicolor emission(visible and near-infrared luminescence) based on visible-light sensitization

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Journal of Rare Earths 2018年第9期36卷 939-945页

作者： Zhijuan Wang Song dang Shu'na Zhao Lining Sun Research Center of nano Science and Technology and School of Material Science and EngineeringShanghai University State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of Saiences

Recently,much attention has been paid to the lanthanide luminescent materials based on the visiblelight sensitization for their potential applications in the fields of bio-imaging and optical *** this work,the lanthanide complexes have been covalently bonded to the ordered mesoporous titania（OMT） matrix,and the resulting titania-based hybrid ordered mesoporous materials（named as LndBOMT,Ln = Eu,Sm,Yb,Nd） were characterized by using Fourier-transform infrared（FT-IR） spectroscopy,small-angle X-ray powder diffraction（SAXd）,N2 adsorption-desorption isotherms,transmission electron microscopy（TEM）,fluorescence spectroscopy,and thermogravimetric ***,exciting with visible light is advantageous over UV *** importance here is that,under excitation with visible light,the LndB-OMT all show characteristic visible（Eu3＋,Sm3＋） as well as nearinfrared（Sm3＋,Yb3＋,Nd3＋） luminescence of the corresponding Ln3＋ ions（multicolor emission covered from 500 to 1400 nm spectral region）,which is attributed to the energy transfer from the ligands to the Ln3＋ ions via an antenna effect.

关键词： Visible-light excitation Visible luminescence Near-infrared luminescence Lanthanide complexes-functionalized Ordered mesoporous titania Rare earths

Biodegradation of Benzo[a]pyrene by Arthrobacter oxydans B4

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Pedosphere 2012年第4期22卷 554-561页

作者： PENG Hui YIN Hua dENG Jun YE Jin-Shao CHEN Shuo-na HE Bao-Yan ZHANG na department of Environmental Engineering Jinan UniversityGuangzhou 510632(China) Key Laboratory of Water//Soil Toxic Pollutants Control and Bioremediation of Guangdong Higher Education Institutes Jinan UniversityGuangzhou 510632(China)

A bacterial strain, Arthrobacter oxydans （B4）, capable of degrading benzo[a]pyrene （BaP） in water body, was isolated from a polycyclic aromatic hydrocarbons-contaminated site. Effects of different factors, such as reaction time, pH value, temperature and organic nutrients, on BaP biodegradation by the strain B4 were studied. After 5 d treatment, the concentration of BaP in mineral salts medium was reduced to 0.318 mg L-1, compared to the initial concentration of 1.000 mg L-1. There was a process of acid formation during the degradation with pH falling from initial 7.01 to 4.61 at 5 d, so keeping the water body under slightly alkaline condition was propitious to BaP degradation. Strain B4 efficiently degraded BaP at 20 to 37 ~C with addition of organic nutrients. The biodegradation and transformation of BaP mainly occurred on cell surfaces, and extracellular secretions played an important role in these processes. Fourier transform infrared spectroscopy and gas chromatograph-mass spectrometer analyses of metabolites showed that ring cleavage occurred in the BaP degradation process and the resulting metabolically utilizable substrates were generated as sole carbon sources for B4 growth. Furthermore, mineralization extent of metabolites was verified by determining the total organic carbon and inorganic carbon in the degradation system.

关键词： degradation mechanism metabolites microbial transformation polycyclic aromatic hydrocarbon