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Dental pulp stem cells stimulate neuronal differentiation of PC12 cells

Neural Regeneration Research 2021年第9期16卷 1821-1828页

作者： Nessma Sultan Laila e.Amin Ahmed R.Zaher mohammed e.grawish Ben A.Scheven School of Dentistry Oral BiologyCollege of Medical and Dental SciencesUniversity of BirminghamBirminghamUK Department of Oral Biology Faculty of DentistryMansoura UniversityEgypt Faculty of Dentistry Horus UniversityNew DamiettaEgypt Department of Oral Biology Faculty of Oral and Dental MedicineDelta University for Science and TechnologyMansouraEgypt

Dental pulp stem cells(DPSCs) secrete neurotrophic factors which may play an important therapeutic role in neural development, maintenance and repair. To test this hypothesis, DPSCs-conditioned medium(DPSCs-CM) was collected from 72 hours serum-free DPSCs cultures. The impact of DPSCs-derived factors on PC12 survival, growth, migration and differentiation was investigated. PC12 cells were treated with nerve growth factor(NGF), DPSCs-CM or co-cultured with DPSCs using Transwell inserts for 8 days. The number of surviving cells with neurite outgrowths and the length of neurites were measured by image analysis. Immunocytochemical staining was used to evaluate the expression of neuronal markers NeuN, microtubule associated protein 2(MAP-2) and cytoskeletal marker βIII-tubulin. Gene expression levels of axonal growth-associated protein 43 and synaptic protein Synapsin-I, NeuN, MAP-2 and βIII-tubulin were analysed by quantitative polymerase chain reaction(qRT-PCR). DPSCs-CM was analysed for the neurotrophic factors(NGF, brain-derived neurotrophic factor [BDNF], neurotrophin-3, and glial cell-derived neurotrophic factor [GDNF]) by specific eLISAs. Specific neutralizing antibodies against the detected neurotrophic factors were used to study their exact role on PC12 neuronal survival and neurite outgrowth extension. DPSCs-CM significantly promoted cell survival and induced the neurite outgrowth confirmed by NeuN, MAP-2 and βIII-tubulin immunostaining. Furthermore, DPSCsCM was significantly more effective in stimulating PC12 neurite outgrowths than live DPSCs/PC12 co-cultures over the time studied. The morphology of induced PC12 cells in DPSCs-CM was similar to NGF positive controls;however, DPSCs-CM stimulation of cell survival was significantly higher than what was seen in NGF-treated cultures. The number of surviving PC12 cells treated with DPSCs-CM was markedly reduced by the addition of anti-GDNF, whilst PC12 neurite outgrowth was significantly attenuated by anti-NGF, an

关键词： brain-derived neurotrophic factor conditioned medium dental pulp stem cell glial cell line-derived nerve growth factor neurite outgrowth neurotrophic factor neurotrophin-3 phaeochromocytoma PC12 cell

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Gingival-derived mesenchymal stem cells:An endless resource for regenerative dentistry

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World Journal of Stem Cells 2018年第9期10卷 116-118页

作者： mohammed e.grawish Department of Oral Biology Faculty of DentistryMansoura University

The gingiva, the masticatory portion of the oral mucosa, is excised and discarded frequently during routine dental treatments and following tooth extraction, dental crown lengthening, gingivectomy and periodontal surgeries. Subsequent to excision, healing eventually happens in a short time period after gingival surgery. Clinically, the gingival tissue can be collected very easily and, in the laboratory, it is also very easy to isolate gingival-derived mesenchymal stem cells (GMSCs) from this discarded gingival tissue. GMSCs, a stem cell population within the lamina propria of the gingival tissue, can be isolated from attached and free gingiva, inflamed gingival tissu-es, and from hyperplastic gingiva. Comparatively, they constitute more attractive alternatives to other dental-derived mesenchymal stem cells due to the availability and accessibility of gingival tissues. They have unique immunomodulatory functions and well-documented self-renewal and multipotent differentiation properties. They display positive signals for Stro-1, Oct-4 and SSeA-4 pluripotency-associated markers, with some co-expre-ssing Oct4/Stro-1 or Oct-4/SSeA-4. They should be considered as the best stem cell source for cell-based therapies and regenerative dentistry. The clinical use of GMSCs for regenerative dentistry represents an attrac-tive therapeutic modality. However, numerous biological and technical challenges need to be addressed prior to considering transplantation approaches of GMSCs as clinically realistic therapies for humans.

关键词： Gingival-derived mesenchymal stem cells Regenerative dentistry Lamina propria of the gingiva Gingiva Stem cell therapy

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Human dental pulp stem/stromal cells in clinical practice

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World Journal of Stem Cells 2024年第2期16卷 54-57页

作者： mohammed e.grawish Department of Oral Biology Faculty of DentistryMansoura UniversityMansoura 35561Egypt Department of Oral Biology Faculty of Oral and Dental MedicineDelta University for Science and TechnologyMansoura 11152Egypt

Dental pulp stem/stromal cells(DPSCs)are fibroblast-like,neural crest-derived,and multipotent cells that can differentiate into several *** are relatively easy to isolate from healthy and inflamed pulps,with little ethical concerns and can be successfully cryopreserved and *** therapeutic effects of DPSCs derived from animal or human sources have been extensively studied through in-vitro and in-vivo animal experiments and the findings indicated that DPSCs are effective not only for dental diseases but also for systemic *** that translational research is a critical step through which the fundamental scientific discoveries could be translated into applicable diagnostics and therapeutics that directly benefit humans,several clinical studies were carried out to generate evidence for the efficacy and safety of autogenous or allogeneic human DPSCs(hDPSCs)as a treatment modality for use in cell-based therapy,regenerative medicine/dentistry and tissue *** clinical medicine,hDPSCs were effective for treating acute ischemic stroke and human exfoliated deciduous teeth-conditioned medium(SHeD-CM)repaired vascular damage of the corpus cavernous,which is the main cause of erectile *** in clinical dentistry,autologous SHeD was able to rege-nerate necrotic dental pulp after implantation into injured teeth,and micrografts enriched with autologous hDPSCs and collagen sponge were considered a treatment option for human intrabony *** contrast,hDPSCs did not add a significant regenerative effect when they were used for the treatment of post-extraction ***-scale clinical studies across diverse populations are still lacking to provide robust evidence on the safety and efficacy of hDPSCs as a new treatment option for various human diseases including dental-related problems.

关键词： Dental pulp stem/stromal cells Human clinical studies Regenerative medicine Regenerative dentistry Cell-based therapy

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early therapeutic effect of platelet-rich fibrin combined with allogeneic bone marrow-derived stem cells on rats’ critical-sized mandibular defects

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World Journal of Stem Cells 2020年第1期12卷 55-69页

作者： Muhammad A Awadeen Fouad A Al-Belasy Laila e Ameen Mohamad e Helal mohammed e.grawish Department of Oral Biology Faculty of Oral and Dental MedicineDelta University for Science and TechnologyMansoura 11152Egypt Department of Oral Surgery and Anesthesia Faculty of Oral and Dental MedicineDelta University for Science and TechnologyMansoura 11152Egypt Department of Oral Biology Faculty of DentistryMansoura UniversityMansoura 35516Egypt

BACKGROUND Critically sized bone defects represent a significant challenge to orthopaedic surgeons *** defects generally result from severe trauma or resection of a whole large *** bone grafts are the current gold standard for the reconstruction of such ***,due to increased patient morbidity and the need for a second operative site,other lines of treatment should be *** find alternative unconventional therapies to manage such defects,bone tissue engineering using a combination of suitable bioactive factors,cells,and biocompatible scaffolds offers a promising new approach for bone *** To evaluate the healing capacity of platelet-rich fibrin(PRF)membranes seeded with allogeneic mesenchymal bone marrow-derived stem cells(BMSCs)on critically sized mandibular defects in a rat *** Sixty-three Sprague Dawley rats were subjected to bilateral bone defects of critical size in the mandibles created by a 5-mm diameter trephine *** were allocated to three equal groups of 21 rats *** I bone defects were irrigated with normal saline and designed as negative *** of group II were grafted with PRF membranes and served as positive controls,while defects of group III were grafted with PRF membranes seeded with allogeneic *** rats from each group were killed at 1,2 and 4 *** mandibles were dissected and prepared for routine haematoxylin and eosin(He)staining,Masson's trichrome staining and CD68 immunohistochemical *** Four weeks postoperatively,the percentage area of newly formed bone was significantly higher in group III(0.88±0.02)than in groups I(0.02±0.00)and II(0.60±0.02).The amount of granulation tissue formation was lower in group III(0.12±0.02)than in groups I(0.20±0.02)and II(0.40±0.02).The number of inflammatory cells was lower in group III(0.29±0.03)than in groups I(4.82±0.08)and II(3.09±0.07).CONCLUSION Bone regenerative quality of critically sized mandibula

关键词： Platelet-rich fibrin membrane Bone marrow-derived stem cells Critical-sized mandibular defects Rats Histological and immunohistochemical staining

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Correction:A Lightweight Approach for Skin Lesion Detection through Optimal Features Fusion

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Computers, Materials & Continua 2025年第1期82卷 1459-1459页

作者： Khadija Manzoor Fiaz Majeed Ansar Siddique Talha Meraj Hafiz Tayyab Rauf mohammed A.el-Meligy Mohamed Sharaf Abd elatty e.Abd elgawad Department of Information Technology University of GujratGujrat50700Pakistan Department of Software engineering University of GujratGujrat50700Pakistan Department of Computer Science COMSATS University Islamabad-Wah CampusWah Cantt47040Pakistan Independent Researcher BradfordBD80HSUK Advanced Manufacturing Institute King Saud UniversityRiyadh11421Saudi Arabia Department of Industrial engineering College of EngineeringKing Saud UniversityRiyadh11421Saudi Arabia

In the article“A Lightweight Approach for Skin Lesion Detection through Optimal Features Fusion”by Khadija Manzoor,Fiaz Majeed,Ansar Siddique,Talha Meraj,Hafiz Tayyab Rauf,mohammed ***-Meligy,Mohamed Sharaf,Abd elatty *** elgawad Computers,Materials&Continua,2022,Vol.70,No.1,pp.1617–***:10.32604/cmc.2022.018621,URL:https://***/cmc/v70n1/44361,there was an error regarding the affiliation for the author Hafiz Tayyab *** of“Centre for Smart Systems,AI and Cybersecurity,Staffordshire University,Stoke-on-Trent,UK”,the affiliation should be“Independent Researcher,Bradford,BD80HS,UK”.

关键词： Fusion Skin Feature

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Therapeutic applications of dental pulp stem cells in regenerating dental,periodontal and oral-related structures

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World Journal of Meta-Analysis 2021年第2期9卷 176-192页

作者： mohammed e.grawish Mahmoud A Saeed Nessma Sultan Ben A Scheven Department of Oral Biology Faculty of DentistryMansoura UniversityMansoura 35516Egypt Department of Oral Biology Faculty of Oral and Dental MedicineDelta University for Science and TechnologyMansoura 11152Egypt School of Dentistry Oral BiologyCollege of Medical and Dental SciencesUniversity of BirminghamBirmingham B57EGUnited Kingdom

Dental pulp stem cells (DPSCs) have emerged as a promising tool with greatpotential for use in tissue regeneration and engineering. Some of the mainadvantages of these cells are their multifaceted differentiation capacity, along withtheir high proliferation rate, a relative simplicity of extraction and culture thatenables obtaining patient-specific cell lines for their use in autologous celltherapy. PubMed, Scopus and Google Scholar databases were searched forrelevant articles related to the use of DPSCs in regeneration of dentin-pulpcomplex (DPC), periodontal tissues, salivary gland and craniomaxillofacial bonedefects. Few studies were found regarding the use of DPSCs for regeneration ofDPC. Scaffold-based combined with DPSCs isolated from healthy pulps was thestrategy used for DPC regeneration. Studies involved subcutaneous implantationof scaffolds loaded with DPSCs pretreated with odontogenic media, or performedon human tooth root model as a root slice. Most of the studies were related toperiodontal tissue regeneration which mainly utilized DPSCs/secretome. Forperiodontal tissues, DPSCs or their secretome were isolated from healthy orinflamed pulps and they were used either for preclinical or clinical *** salivary gland regeneration, the submandibular gland was the onlymodel used for the preclinical studies and DPSCs or their secretome were isolatedonly from healthy pulps and they were used in preclinical studies. Likewise,DPSCs have been studied for craniomaxillofacial bone defects in the form ofmandibular, calvarial and craniofacial bone defects where DPSCs were isolatedonly from healthy pulps for preclinical and clinical studies. From the previousresults, we can conclude that DPSCs is promising candidate for dental and oraltissue regeneration.

关键词： Dental pulp stem cells Dentin-pulp complex Periodontal tissues Salivary glands Cell-based therapy Cell-free therapy

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Dental pulp stem cells: Novel cell-based and cell-free therapy for peripheral nerve repair

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World Journal of Stomatology 2019年第1期7卷 1-19页

作者： Nessma Sultan Laila e Amin Ahmed R Zaher Ben A Scheven mohammed e.grawish Department of Oral Biology Faculty of Dentistry Mansoura University School of Dentistry Oral Biology College of Medical and Dental SciencesUniversity of Birmingham

The regeneration of peripheral nerves comprises complicated steps involving a set of cellular and molecular events in distal nerve stumps with axonal sprouting and remyelination. Stem cell isolation and expansion for peripheral nerve repair(PNR) can be achieved using a wide diversity of prenatal and adult tissues, such as bone marrow or brain tissues. The ability to obtain stem cells for cell-based therapy(CBT) is limited due to donor site morbidity and the invasive nature of the harvesting process. Dental pulp stem cells(DPSCs) can be relatively and simply isolated from the dental pulps of permanent teeth, extracted for surgical or orthodontic reasons. DPSCs are of neural crest origin with an outstanding ability to differentiate into multiple cell lineages. They have better potential to differentiate into neural and glial cells than other stem cell sources through the expression and secretion of certain markers and a range of neurotropic factors;thus, they should be considered a good choice for PNR using CBT. In addition,these cells have paracrine effects through the secretion of neurotrophic growth factors and extracellular vesicles, which can enhance axonal growth and remyelination by decreasing the number of dying cells and activating local inhabitant stem cell populations, thereby revitalizing dormant or blocked cells,modulating the immune system and regulating inflammatory responses. The use of DPSC-derived secretomes holds great promise for controllable and manageable therapy for peripheral nerve injury. In this review, up-to-date information about the neurotrophic and neurogenic properties of DPSCs and their secretomes is provided.

关键词： Dental pulp stem cells Secretomes Cell-based therapy Cell-free therapy Peripheral nerve injury

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Novel Framework for an Intrusion Detection System Using Multiple Feature Selection Methods Based on Deep Learning

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Tsinghua Science and Technology 2024年第4期29卷 948-958页

作者： A.e.M.eljialy mohammed Yousuf Uddin Sultan Ahmad Department of Information Systems College of Computer Engineering and SciencesPrince Sattam Bin Abdulaziz UniversityAlkharjSaudi Arabia Department of Computer Science College of Computer Engineering and SciencesPrince Sattam Bin Abdulaziz UniversityAlkharjSaudi Arabiaand also with University Center for Research and Development(UCRD)Department of Computer Science and EngineeringChandigarh UniversityPunjabIndia

Intrusion detection systems (IDSs) are deployed to detect anomalies in real time. They classify a network’s incoming traffic as benign or anomalous (attack). An efficient and robust IDS in software-defined networks is an inevitable component of network security. The main challenges of such an IDS are achieving zero or extremely low false positive rates and high detection rates. Internet of Things (IoT) networks run by using devices with minimal resources. This situation makes deploying traditional IDSs in IoT networks unfeasible. Machine learning (ML) techniques are extensively applied to build robust IDSs. Many researchers have utilized different ML methods and techniques to address the above challenges. The development of an efficient IDS starts with a good feature selection process to avoid overfitting the ML model. This work proposes a multiple feature selection process followed by classification. In this study, the Software-defined networking (SDN) dataset is used to train and test the proposed model. This model applies multiple feature selection techniques to select high-scoring features from a set of features. Highly relevant features for anomaly detection are selected on the basis of their scores to generate the candidate dataset. Multiple classification algorithms are applied to the candidate dataset to build models. The proposed model exhibits considerable improvement in the detection of attacks with high accuracy and low false positive rates, even with a few features selected.

关键词： random forest feature selection decision tree software-defined network logistic regression intrusion detection system XGB classifier AdaBoost

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COVID-19 Automatic Detection Using Deep Learning

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Computer Systems Science & engineering 2021年第10期39卷 15-35页

作者： Yousef Sanajalwe mohammed Anbar Salam Al-e’mari National Advanced IPv6 Centre of excellence(Nav6) Universiti Sains Malaysia11800 USMPenangMalaysia Computer Science Department Northern Border University(NBU)9280 NBUAr’arthe Kingdom of Saudi Arabia

The novel coronavirus disease 2019(COVID-19)is a pandemic disease that is currently affecting over 200 countries around the world and impacting billions of *** first step to mitigate and control its spread is to identify and isolate the infected ***,because of the lack of reverse transcription polymerase chain reaction(RT-CPR)tests,it is important to discover suspected COVID-19 cases as early as possible,such as by scan analysis and chest X-ray by ***,chest X-ray analysis is relatively time-consuming since it requires more than 15 minutes per *** this paper,an automated novel detection model of COVID-19 cases is proposed to perform real-time detection of COVID-19 *** proposed model consists of three main stages:image segmentation using Harris Hawks optimizer,synthetic image augmentation using an enhanced Wasserstein And Auxiliary Classifier Generative Adversarial Network,and image classification using Conventional Neural *** chest X-ray images datasets are used to train and test the proposed *** demonstrate that the proposed model is very efficient in the automatic detection of COVID-19 positive *** achieved 99.4%accuracy,99.15%precision,99.35%recall,99.25%F-measure,and 98.5%specificity.

关键词： Conventional neural network COVID-19 deep learning enhanced Wasserstein and auxiliary classifier generative adversarial network image classification image segmentation chest x-rays

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Adaptive expanding Ring Search Based Per Hop Behavior Rendition of Routing in MANeTs

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Computers, Materials & Continua 2021年第4期67卷 1137-1152页

作者： Durr-e-Nayab Mohammad Haseeb Zafar mohammed Basheri Faculty of electrical and Computer engineering University of Engineering and TechnologyPeshawar25120Pakistan Faculty of Computing and Information Technology King Abdulaziz UniversityJeddah21589Saudi Arabia

Routing protocols in Mobile Ad Hoc Networks(MANeTs)operate with expanding Ring Search(eRS)mechanism to avoid ooding in the network while tracing *** mechanism searches the network with discerning Time to Live(TTL)values described by respective routing protocol that save both energy and *** work exploits the relation between the TTL value of a packet,trafc on a node and eRS mechanism for routing in MANeTs and achieves an Adaptive eRS based Per Hop Behavior(AeRSPHB)rendition of requests *** search request is classied based on eRS attributes and then processed for routing while monitoring the node *** algorithms are designed and examined for performance under exhaustive parametric setup and employed on adaptive premises to enhance the performance of the *** network is tested under congestion scenario that is based on buffer utilization at node level and link utilization via back-off stage of Carrier Sense Multiple Access with Collision Avoidance(CSMA/CA).Both the link and node level congestion is handled through retransmission and rerouting the packets based on eRS *** aim is to drop the packets that are exhausting the network energy whereas forward the packets nearer to the destination with *** simulations are carried out for network scalability,node speed and network terrain *** results show that the proposed models attain evident performance enhancement.

关键词： expanding ring search mobile ad hoc networks multi hop wireless networks on-demand ad hoc networks per hop behavior quality of servi

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