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Diagnosis of Clinical Cases of Infectious Bursal Disease Using a Modified Rapid Taq Man-MGB Real-Time RT-PCR Assay

Journal of Agricultural Science and Technology(A) 2018年第4期8卷 230-238页

作者： Maryame Cheggag Khalil Zro Ghizlane Sebbar Naoufal Rahmatallah Mohamed Mouahid mohammed EL Houadfi Faouzi Kichou Unit of Histology and Pathological Anatomy Department of Veterinary Pathology and Public HealthInstitute of Agronomy and Veterinary Medicine Hassan IIB.P.6202RabatMorocco Society of Veterinary Pharmaceutical and Biological Productions(Biopharma) B.P.4569RabatMorocco Veterinary Clinic Temara Morocco

Infectious bursal disease(IBD)is an important contagious viral infection of immune system of *** infection possesses a permanent threat to the profitability of poultry industry *** aim of this work was to modify the Taq Man-MGB real-time reverse transcription-polymerase chain reaction(rRT-PCR)in one step involving two fluorogenic Taq Man labeled probe and using this protocol for detection of infectious bursal disease virus(IBDV)collected from suspected cases distributed in different regions of the country during the period *** intralaboratory validation of modified method was realized for specificity,linearity,repeatability,sensitivity and *** allowed reducing the test running time by six *** method was applied on 102 pools of bursa of fabricius(BF)samples collected from affected broiler farms suspected to be infected by *** showing macroscopic lesions including muscle petechial hemorrhages,hypertrophy and hemorrhage of BF,were subjected to molecular analysis using modified protocol“Taq Man-MGB rRT-PCR”.The validation satisfied all criteria and the assay developed could be a useful tool for a very rapid diagnosis of IBDV and permit to detect and to discriminate in one-step very virulent(vv)from non-vv(classic and variant)IBDV *** of 84 IBDV positive samples,a prevalence of 39%for vv strains and 61%for classical strains was *** results indicate that despite the vaccination against IBDV,the vv form of this pathologie continues to cause serious problems for Moroccan broiler *** obtained results indicate the successfully detection of IBDV and differentiated all vvIBDV strains from non-vvIBDV strains;Avian infectious agent RNA viruses tested are negative,demonstrating great specificity of the *** results obtained indicate that this method is suitable as a routine laboratory test for the rapid detection and differentiation of IBDV strains in samples of avian origin.

关键词： IBDV one-step rRT-PCR Taq Man-MGB macroscopic lesions

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Epidemiological Characteristics of COVID-19 Confirmed Cases in Muscat Governorate, Sultanate of Oman

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Open Journal of Epidemiology 2021年第1期11卷 56-69页

作者： Lamya Al Balushi Fatma Al Fahdi Thamra Al Ghafri mohammed Amin Jeffrey Singh Balqees Al Siyabi Mariam Al Kalbani Maya Al Kindi Fatma Al Balushi Haleema Al Ghazaili Malak Al Alwai Salwa Al Mashari Hanan Al Kindi Ahlam Al Rumhi Ibtisam Al Shidhani Zainb Al Balushi Padmamohan J. Kurup Fatma Al Ajmi Disease Surveillance and Control Directorate General for Health Services Muscat Governorate Ministry of Health Muscat Oman Directorate General for Health Services Muscat Governorate Ministry of Health Muscat Oman Primary Health Care Directorate General for Health Services Muscat Governorate Ministry of Health Muscat Oman Directorate General of Khoula Hospital Muscat Oman Directorate General for Disease Surveillance and Control Ministry of Health Muscat Oman

Introduction: The coronavirus disease 2019 (COVID-19) was declared as pandemic by WHO by March 11th. First case reported in Oman was on 24th February 2020 and later the country went through stages of epidemic progression. This study describes the sociodemographic and epidemiological characteristics of confirmed COVID-19 cases in Muscat governorate and related outcomes. Materials and Methods: This is a descriptive, exploratory analysis of all lab confirmed COVID 19 cases that were reported from 1st February to 31st May 2020. Data for the study was primarily extracted from notifications system established for surveillance (Tarassud). Secondary data sources were, contact listings and hospital medical records. Results: 11,648 initial cases of confirmed COVID-19 infections were included. The mean age was 35 years, 84.7% (N = 9862) were males, 25.9% (N = 3017) were Omanis, and 74.1% (N = 8631) were expatriates of which Indian origin were the majority (37%). Fever and cough were the most common presentations (46.3% and 29.5% respectively). Diabetes and hypertension were the most common comorbidities (4.9% and 4.6% respectively). Hospital admission was required for 7% (N = 811) of the total reported cases, out of them 171 cases (21%) were admitted to ICU, where 107 (13.2%) were ventilated. The case fatality rate (CFR) was 0.9%. 158 clusters containing 2949 contacts were identified from case records and categorised into 3 groups based on their exposure settings. The incubation period measured was 8 days (IQR 4.0 - 15.0) for workplace, 8 days (IQR 4 - 17) for dormitory and 4 days (IQR 2.0 - 7.0) for family groups. The secondary attack rate (SAR) estimated was 41.6% (95% CI: 0.34 - 0.48), 52% (95% CI: 0.40 - 0.63) and 33% (95% CI: 0.27 - 0.38) for workplace, dormitory and family groups, respectively. Conclusion: Results of this study, determine the transmission trend of COVID-19 in a country with high immigran

关键词： COVID-19 Epidemiological Characteristics Infectious Disease Cluster

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积雪草皂苷与曲安奈德单独及联合治疗兔耳模型瘢痕的疗效

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黑龙江医药科学 2014年第4期37卷 20-22页

作者：阿迪米孙明李善昌佳木斯大学附属第二医院口腔医院黑龙江佳木斯154002

目的:研究积雪草皂苷和曲安奈德单独及联合治疗兔耳模型瘢痕的疗效。方法:在20只新西兰兔的耳朵上造成增生性瘢痕,术后第28天开始治疗,新西兰兔分为4组,第1组使用病灶内曲安奈德(TAC)治疗,第2组使用积雪草皂苷治疗,第3组使用两者联合治... 详细信息

目的:研究积雪草皂苷和曲安奈德单独及联合治疗兔耳模型瘢痕的疗效。方法:在20只新西兰兔的耳朵上造成增生性瘢痕,术后第28天开始治疗,新西兰兔分为4组,第1组使用病灶内曲安奈德(TAC)治疗,第2组使用积雪草皂苷治疗,第3组使用两者联合治疗,第4组为控制组。在术后第56天,处死所有新西兰兔,收集伤疤,并使用苏木精-伊红(HE)染色、马松三色染色和CD34免疫组织化学进行处理。结果:各组的ETI如下:组1、2和3,相比对照组,分别下降61.2%、21%和43.3%。SEI下降如下:组1、2和3,相比对照组,分别为40.3%、18.7%和33.3%。马松染色表明,各研究组胶原纤维减少;更细,更有规律。此外,CD34染色表明,各研究组微脉管形成也均减少,检测各组CD34阳性细胞,检测值如下:组1(10.00±7.04),组2(32.5±22.15),组3(20.67±15.83)和组4(43.83±23.95)。结论:TAC比积雪草皂苷和两种药物的联合效果更好,但是两种药物联合比单独使用各个药物表现出更好的胶原排列。

关键词：增生性瘢痕曲安奈德积雪草皂苷

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Assessment of the Cost-returns and Profitability Patterns of Tomato Production in Yamaltu-Deba Local Government Area of Gombe State,Nigeria

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Journal of Economic Science Research 2021年第3期4卷 1-9页

作者： SALEH Abdullahi JIBO mohammed Ibrahim AHMAD Muhammad El-hafeez BAKO Bulus Danladi MUHAMMAD Abbas Muhammad Department of Agricultural Economics and Extension Federal University of Kashere Gombe StateNigeria

The study examined profitability of tomato production in Yamaltu-Deba Local Government Area of Gombe State.A three-stage sampling technique was used to select 96 tomato *** were collected using a structured questionnaire and were analysed using descriptive statistics,farm budget model,and t-test *** results revealed that,the mean age of tomato producers was 38.94 years,92.48%were males,71.56%were married with the majority(95.44%)had family size ranging from 1-6 persons,and had 6.55 mean years of farming experience,having an average of 0.6 ha farm size ***,the result revealed that only 8.74%that have attained tertiary *** results also revealed average variable costs constituted 88.98%and 88.84%of the average total costs of production in the dry and rainy seasons *** per hectare average net income realised were found to be₦154,444.20($398.05)and₦39,725.14($102.38)in the dry and rainy seasons ***,the returns per naira invested was₦0.67($0.00173)in dry season and₦0.18($0.00046)in rainy season(P<0.05).Moreover,the results revealed positive and desirable gross and operating ratios of<1;implying the tomato farms in the study area maintained profitability levels both in the short and long ***,inadequate capital was critical;which was attributed to lack of affordable sources of *** of storage and processing facilities were among the impediments to large scale tomato production in the study ***,improvement in the existing patterns and as well as the provision of adequate essential factors of production will help expand the present scale of ***,governments and other financial institutions should do more to provide soft loans to the farmers to improve efficiency.

关键词： Profitability Assessment Tomato Production Yamaltu-Deba Gombe

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干旱区城市化对城市内河水质的影响研究——以吐曼河为例

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水土保持研究 2007年第3期14卷 188-191页

作者：艾尼瓦尔.买买提麦麦提吐尔逊.艾则孜海米提.依米提新疆大学资源与环境科学学院干旱半干旱区可持续发展国际研究中心乌鲁木齐830046

随着城市社会经济的迅速发展，工业主导的城市化过程带来了许多城市生态环境问题，引起了城市内河水质的恶化。采用1996～2000年喀什市吐曼河水质观测数据、1990年与1999年卫星遥感图像和城市发展中有关的社会经济统计数据与土地利用资... 详细信息

随着城市社会经济的迅速发展，工业主导的城市化过程带来了许多城市生态环境问题，引起了城市内河水质的恶化。采用1996～2000年喀什市吐曼河水质观测数据、1990年与1999年卫星遥感图像和城市发展中有关的社会经济统计数据与土地利用资料，在3S技术的支持下，运用地表水污染综合指数法，对吐曼河水质进行评价，定量与定性相结合分析了干旱区城市化对城市内河水质的影响。结果表明：大气中的总悬浮颗粒是导致吐曼河水质严重污染的主要原因。此外，城市化所带来得人口增加、工业化与土地利用方式的改变也一定程度的影响了吐曼河的水质。

关键词：吐曼河城市化水质影响

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In Vitro Development and Germination of Immature Embryos of Sweet Oranges Parson Brown and Pineapple(Citrus sinensis)

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Journal of Agricultural Science and Technology(B) 2018年第7期8卷 405-419页

作者： Karim Mahmoudi Najat Handaji mohammed Ibriz Najat Arsalane Tarik Aderdour Kawtar Label Hanae Ennaciri Hamid Benyahya Laboratory for the Improvement and Conservation of Plant Genetic Resources National Institute of Agronomic Research(INRA)KenitraBP 257Morocco Laboratory of Genetics and Biometry Faculty of SciencesUniversity Ibn TofailKenitraMorocco

Oranges are classified as the most traded fruit variety *** diversification of the varietal profile of this group is among the objectives of the Citrus breeding ***,the creation of new triploid hybrids via immature embryos rescue,can lead to an increase in gene pool and produce seedless *** addition,flow cytometry was successfully used for analyzing ploidy levels and genetic origin of triploid *** present study was aimed at optimizing immature embryos in vitro germination and plantlet development based on medium chemical composition of the two orange varieties,Pineapple and Parson *** the fruit gets matured,they are harvested and the seeds extracted are classified according to their *** those which are small are cultured in a sterile Murashige and Tucker(MT)basal *** different concentrations of growth regulators are put to the test in order to gain the best medium for seeds development.M1(MT+1 mg/L gibberellic acid(GA3)),M2(MT+1 mg/L kenitin+0.5 mg/L 6-benzylaminopurine(BAP)+0.1 naphthalene acetic acid(NAA)),M3(MT+25 mg/L adenine sulfate(ADS)),M4(MT+0.5 mg/L kenitin+0.5 mg/L BAP+1 mg/L GA3).Percentage of germinated embryos(PGE)is the highest in M3 with 100%and 90%for both Pineapple and Parson Brown varieties,*** addition,the germination interval average(GIA)is shorter in M1 for both varieties,which takes 6 d and 7 d,*** regard to speed seedling growth(mm/week),both varieties undergo significant change in all four ***,the highest rate of acclimatization for the Pineapple and Parson Brown varieties is 80%and 90%in *** general,the smaller the embryos are,the more sensitive they are to culture medium *** is therefore essential to optimize the medium components in order to stimulate their in vitro ***,M1(MT+1 mg/L GA3)remains the best to promote short term good germination and produce a better rate of acclimatization.

关键词： Citrus orange triploids in vitro germination immature embryos flow cytometry genetic

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Three-Point Bending Test and Crack Detection by Acoustic Emission on Different Spring Steel Wires with Different Crystallographic Textures

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材料科学与工程（中英文A版） 2023年第3期13卷 53-67页

作者： Mathias Lorenz mohammed Salih Daniela Schwerdt Nowfal Al-Hamdany Emad Maawad Norbert Schell Eckehard Müller University of Wismar Faculty of Mechanical EngineeringPh.-Müller-Straße 14Wismar D-23966Germany Helmholtz Zentrum Hereon Institute of Materials MechanicsDepartment of Laser Processing and Structural AssessmentMax-Planck Str.1Geestacht D-21502Germany Helmholtz Zentrum Hereon Institute of Materials PhysicsMax-Planck-Str.1Geesthacht D-21502Germany Hochschule Bochum Department of Mechatronics and Mechanical EngineeringAm Hochschulcampus 1Bochum D-44801Germany Steinbeis-Transfercenter of Springtechnology Part behaviour und ProcessLangerfeldstr.53 cIserlohn D-58638Germany

In the production of compression springs,high forming velocities and grades of deformation during winding and setting may induce cracks that can lead to failure causing risks of an accident and *** AE(acoustic emission)technology,a non-destructive monitoring method,can detect acoustic signals reflected from *** establish this method in the production of technical springs,it was necessary,to find out whether the AE signal is influenced by material properties,phase fractions distribution from tempered martensite,retained austenite,and microstructure including crystallographic *** addition,it was investigated to what extent the detected AE signal can be useful to separate between an actual crack and other material *** an in-situ three-point bending test with the AE technology,macro-and micro-crack-typical AE signals were detected for five different spring steel wires(SH,VDSiCr,and FDSiCr according to EN-10270-1 and EN-10270-2).The relative energy related to the initiation,propagation,and growth of cracks caused by mechanical stress was measured using a piezoelectric *** a crack AE signal appeared for the first time,the bending tests were stopped *** results show that the frequency spectrum combined with the intensity of the acoustic signals generated during crack growth depends on the material properties and the crystallographic ***,it could be shown that it is possible to differentiate between micro-crack-typical AE signals and other signals that result from different material responses.

关键词： Technical springs AE analysis micro-computer tomography texture

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我国羊泰勒虫病快速诊断方法成环恒温扩增法的研制

我国羊泰勒虫病快速诊断方法成环恒温扩增法的研制

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中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十次学术研讨会

作者：刘志杰侯俊玲 mohammed A.Bakheit Diaeldin A.salih 罗建勋殷宏 Jabbar S.Ahmed Ulrike Seitzer 中国农业科学院兰州兽医研究所国家兽医病原生物学重点实验室甘肃省兽医寄生虫病重点实验室 Division of Veterinary Infection Biology and Immunology Research Center BorstelGermany National Research Center for Protozoan Disease Obihiro University for Agriculture and Veterinary MedicineInada-choObihiroHokkaidoJapan 中国农业科学院兰州兽医研究所国家兽医病原生物学重点实验室甘肃省兽医寄生虫病重点实验室

成环恒温扩增方法(LAMP)是一个新型的核酸检测方法,该方法需要两对或三对引物与目标DNA模板结合,在恒温条件下反应,这一方法具有高效,敏感和特异等特点。本研究中,我们以引起我国羊泰勒虫病的病原尤氏泰勒虫(Theileria uilenbergi)和吕...

成环恒温扩增方法(LAMP)是一个新型的核酸检测方法,该方法需要两对或三对引物与目标DNA模板结合,在恒温条件下反应,这一方法具有高效,敏感和特异等特点。本研究中,我们以引起我国羊泰勒虫病的病原尤氏泰勒虫(Theileria uilenbergi)和吕氏泰勒虫(***)同源基因(UTRu6,UTRlu8)的保守区为模板,设计了LAMP引物。这组引物能同时扩增这两种病原,反应条件为63摄氏度,反应时间为15分钟。反应产物经EcoRⅠ酶切消化和序列测定证明了反应的特异性,另外,通过测定10倍稀释的DNA样品,证明了该方法具有从0.1皮克DNA样品中扩增到目标基因的敏感性。最后,通过比较LAMP和反向线性应迹法(RLB)测定86份野外样品的结果,确定了LAMP的敏感性和特异性分别为66.0%和97.4%。由于该方法快速,简便,廉价,敏感且特异,所以它将具有对我国羊泰勒虫病的诊断和流行病学调查的潜在应用价值。

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Burnout during pandemic COVID-19 in Saudi and non-Saudi nurses in King Abdulaziz hospital,Makkah

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Nursing Communications 2023年第1期7卷 1-11页

作者： Majda Rashed Khairi Mashael Abdulnasser Ahmad Abdulrahman mohammed Salah Alshmemri May Hassan Bagadood Sanaa Awwad Alsulami Faculty of Nursing Umm Al-Qura UniversityMakkah 24382Saudi Arabia

Background:COVID-19 put the global health system in a disastrous *** plays a vital role in healthcare *** ratio of burnout increased during this *** the context of Saudi Arabia,nurses’whether these are Saudi or non-Saudi the burnout due to emotional exhaustion,depersonalization,and personal accomplishment,the situation of burnout could be *** is,therefore,important to understand the phenomenon of nurse burnout and the factors that contribute to *** study aims to understand burnout among nurses and the factors that affect nurses during the COVID-19 ***:The design of this study was quantitative cross-sectional and *** study population included 255 nurses working in the King Abdulaziz hospital,Makkah,in ***-administered questionnaire(google forms)was distributed through email and *** analysis system version 9.4 for data analysis and ***:Most of the nurse participants were in the age range 31-40,were females and were Saudi nationals.A Chi-square analysis showed a significant burnout level on the sub-scale of emotional exhaustion and personal accomplishment,while a partial burnout level was observed on the sub-scale of *** level of burnout was higher and more significant among Saudi nurses compared to non-Saudi *** impact of demographic variables on burnout showed that nationality,level of education,and duty type were the most influential and significant variables in burnout among Saudi and non-Saudi ***:The findings indicated that nurses’burnout is higher during COVID-19 and is closely related to their working *** addition,when nurses are more nervous and depressed,a higher level of burnout will be *** depression and frustration are influenced by working hours,attention should be given to this factor,focusing on interventions to alleviate the causes that lead to nurses’burnout.

关键词： burnout COVID-19 nurses Saudi nurses non-Saudi nurses

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基于需求和自我效能理论构建研究生英语分级教学新模式

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中国地质教育 2014年第1期23卷 58-61页

作者：安静茹克叶.穆罕默德许秀妍高平中国地质大学(北京)外国语学院北京100083

分级教学改革旨在解决公共研究生英语教学中存在的问题,改革无论学生英语水平如何、需求如何,都使用同一本教材、采用同一种教学模式、教授同一种课型的方式。本文采取针对硕士研究生的不同英语水平和不同主体关于英语学习的不同需求进... 详细信息

分级教学改革旨在解决公共研究生英语教学中存在的问题,改革无论学生英语水平如何、需求如何,都使用同一本教材、采用同一种教学模式、教授同一种课型的方式。本文采取针对硕士研究生的不同英语水平和不同主体关于英语学习的不同需求进行"有的放矢"、"因材施教"的教学新模式,目标是缩小研究生的英语语言知识与实际应用能力之间的差距,促进学生英语能力与其本领域的研究相结合。

关键词：需求自我效能分级教学应用能力

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