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Identifying the stability of housekeeping genes to be used for the quantitative real-time PCR normalization in retinal tissue of streptozotocin-induced diabetic rats

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International Journal of Ophthalmology(English edition) 2024年第5期17卷 794-805页

作者： Muhammad Zulfiqah Sadikan Nurul Alimah Abdul Nasir mohammad Johari Ibahim Igor Iezhitsa Renu Agarwal Department of Pharmacology Faculty of MedicineManipal University College Malaysia(MUCM)Bukit BaruMelaka 75150Malaysia Centre for Neuroscience Research(NeuRon) Faculty of MedicineUniversiti Teknologi MARASungai Buloh CampusSungai BulohSelangor 47000Malaysia Department of Medical Education Faculty of MedicineUniversiti Teknologi MARASungai Buloh CampusSungai BulohSelangor 47000Malaysia Department of Biochemistry and Molecular Medicine Faculty of MedicineUniversiti Teknologi MARASungai Buloh CampusSungai BulohSelangor 47000Malaysia School of Medicine International Medical UniversityBukit JalilKuala Lumpur 57000Malaysia Department of Pharmacology and Bioinformatics Volgograd State Medical UniversityVolgograd 400131Russia

AIM:To investigate the stability of the seven housekeeping genes:beta-actin(ActB),glyceraldehyde-3-phosphate dehydrogenase(GAPDH),18s ribosomal unit 5(18s),cyclophilin A(CycA),hypoxanthine-guanine phosphoribosyl transferase(HPRT),ribosomal protein large P0(36B4)and terminal uridylyl transferase 1(U6)in the diabetic retinal tissue of rat ***:The expression of these seven genes in rat retinal tissues was determined using real-time quantitative reverse transcription polymerase chain reaction(RT-qPCR)in two groups;normal control rats and streptozotocininduced diabetic *** stability analysis of gene expression was investigated using geNorm,NormFinder,BestKeeper,and comparative delta-Ct(ΔCt)***:The 36B4 gene was stably expressed in the retinal tissues of normal control animals;however,it was less stable in diabetic *** 18s gene was expressed consistently in both normal control and diabetic rats’retinal *** this gene was the best reference for data normalisation in RT-qPCR studies that used the retinal tissue of streptozotocin-induced diabetic ***,there was no ideal gene stably expressed for use in all experimental ***:Identifying relevant genes is a need for achieving RT-qPCR validity and reliability and must be appropriately achieved based on a specific experimental setting.

关键词： housekeeping genes stability real-time reverse transcription polymerase chain reaction retinal tissue streptozotocin-induced diabetic rats

Short circuit analysis of a fault-tolerant current-limiting high temperature superconducting transformer in a power system in presence of distributed generations

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Superconductivity 2024年第1期9卷 74-86页

作者： Alireza Sadeghi Shahin Alipour Bonab Wenjuan Song mohammad Yazdani-Asrami Propulsion Electrification&Superconductivity GroupAutonomous Systems&Connectivity DivisionJames Watt School of EngineeringUniversity of GlasgowGlasgow G128QQUnited Kingdom

Power transformers are key elements for the safe and reliable delivery of electrical energy generated by renewable energy resources to consumers via transmission ***‐tolerant current‐limiting High Temperature Superconducting(FTCL HTS)transformers are type of superconducting transformers that tolerate fault for seconds and limit the fault current without the threat of burnout or delamination of tapes and deformation of *** this paper,the fault performance of a FTCL HTS transformer in a standard IEEE power system is *** studied transformer is a 50 MVA 132 kV/13.8 kV transformer where both windings are made up of HTS *** understudied power system consists of two microgrids with distributed *** of the power in microgrids is supplied by the upstream grid which is connected to the microgrids through the HTS *** fault scenarios have been considered in this power system,in each one of these scenarios,a fault happens in one of the *** considered fault scenarios have an approximate fault current of 18x to 23x of the rated current in the secondary *** showed that insulated windings in FTCL HTS transformers could substantially reduce the peak temperature of the HTS windings,compared to bare ***,post‐fault loading is imposed on the HTS windings,to observe their performance against the current increase after fault *** this case,for the first scenario of the faults,the FTCL HTS transformer could tolerate 192%of post‐fault overloading,while this number for the second fault scenario is 170%.Finally,the impact of post‐fault loading on the full recovery time was discussed.

关键词： Fault Energy Heat dissipation Heat transfer Insulation Recovery Time Temperature rise

Geometric investigation of entrance proportions of houses from the Qajar to the beginning of the early Pahlavi in Dezful City(1789—1979)

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Frontiers of Architectural Research 2024年第1期13卷 57-78页

作者： Raziyeh Rezadoost Dezfuli mohammad Mehrakizadeh Behnaz Safar Ali Najar Hassan Bazazzadeh mohammadjavad Mahdavinejad Department of Architecture Poznan University of Technology61-131 PoznanPoland Department of Architecture and Urban Planning Jundi Shapur University of TechnologyDezfulIran College of Engineering and Architecture University of Nizwa616 Birkat Al MawzOman Department of Architecture Faculty of Art and ArchitectureTarbiat Modares UniversityTehranIran

Due to the importance of the historical context of Dezful City,investigating historic buildings is *** unfortunately,a large part of it is being *** this reason,it is essential to discover the guidelines and logic behind the architectural design of these buildings to help their reconstruction or ***,there is no logical information about the components of this context and the reconstructions are usually done according to taste and based on architecture tested or personal ***,it is necessary to find a logic behind these critical components that support the whole plan or parts of it in a particular *** purpose of this research is to discover and find the logic behind the design of this building in the historical context of *** geometrical proportions can significantly help to reconstruct buildings better and more *** this purpose,30 historical houses of Dezful were randomly selected,and their input information was drawn in 2D form in ***,the length,width,and height proportions were measured,and the obtained ratios were compared with the golden proportions and dynamic *** paper studies entrance based on ratios of√2,√3,√4,√4,√1.25 andφ(the golden section).The results show that golden proportions and proportions derived from dynamic rectangles are present in the wording of all ***,the range of ratios in the plan is from 1.34 to 2,and the content of changes in the section is from 1.22 to *** results can help architects,and particularly building owners,to reconstruct based on existing logic by discovering the proportions of the entrances of the *** is possible to administer a part of the damaged tissue and estimate its dimensions and sizes because the proportions’completeness can help revive half-destroyed historical buildings.

关键词： Proportions Historical houses House entrance Dezful city Qajar period to early Pahlavi

Classification and Comprehension of Software Requirements Using Ensemble Learning

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Computers, Materials & Continua 2024年第8期80卷 2839-2855页

作者： Jalil Abbas Arshad Ahmad Syed Muqsit Shaheed Rubia Fatima Sajid Shah mohammad Elaffendi Gauhar Ali School of Computer Science and Technology Anhui UniversityHefei230039China School of Computing Sciences Pak Austria FachhochschuleInstitute of Applied Sciences and TechnologyHaripur22620Pakistan Department of Computer Science and IT University of LahoreLahore55150Pakistan Department of Computer Science Emerson UniversityPunjabMultan60000Pakistan EIAS(Emerging Intelligent Autonomous Systems)Data Science Lab Prince Sultan UniversityRiyad12435Saudi Arabia

The software development process mostly depends on accurately identifying both essential and optional ***,user needs are typically expressed in free-form language,requiring significant time and human resources to translate these into clear functional and non-functional *** address this challenge,various machine learning(ML)methods have been explored to automate the understanding of these requirements,aiming to reduce time and human ***,existing techniques often struggle with complex instructions and large-scale *** our study,we introduce an innovative approach known as the Functional and Non-functional Requirements Classifier(FNRC).By combining the traditional random forest algorithm with the Accuracy Sliding Window(ASW)technique,we develop optimal sub-ensembles that surpass the initial classifier’s accuracy while using fewer *** results demonstrate that our FNRC methodology performs robustly across different datasets,achieving a balanced Precision of 75%on the PROMISE dataset and an impressive Recall of 85%on the CCHIT *** datasets consistently maintain an F-measure around 64%,highlighting FNRC’s ability to effectively balance precision and recall in diverse *** findings contribute to more accurate and efficient software development processes,increasing the probability of achieving successful project outcomes.

关键词： Ensemble learning machine learning non-functional requirements requirement engineering accuracy sliding window

Tilt Angle Optimality Criteria for Stand Alone PV Systems

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Journal of Power and Energy Engineering 2024年第3期12卷 1-18页

作者： mohammad Abu-Naser Faculty of Engineering and Technology Philadelphia University Amman Jordan

The conventional approach to optimizing tilt angles for fixed solar panels aims to maximize energy generation over the entire year. However, in the context of a supply controlled electric grid, where solar energy availability varies, this criterion may not be optimal. This study explores two alternative optimization criteria focused on maximizing baseload supply potential and minimizing required storage capacity to address seasonality in energy generation. The optimal tilt angles determined for these criteria differed significantly from the standard approach. This research highlights additional factors crucial for designing solar power systems beyond gross energy generation, essential for the global transition towards a fully renewable energy-based electric grid in the future.

关键词： Electric Grid Fixed Solar Panels Optimal PV Tilt Angle Seasonal Solar Variability Renewable Energy Supply-Demand Balance

Choosing Multimodal Freight Mix: An Integrated Multi-Objective Multicriteria Approach

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Journal of Transportation Technologies 2024年第3期14卷 402-422页

作者： mohammad Anwar Rahman School of Engineering Science & Technology Central Connecticut State University New Britain CT USA

Multimodal freight transportation emerges as the go-to strategy for cost-effectively and sustainably moving goods over long distances. In a multimodal freight system, where a single contract includes various transportation methods, businesses aiming for economic success must make well-informed decisions about which modes of transport to use. These decisions prioritize secure deliveries, competitive cost advantages, and the minimization of environmental footprints associated with transportation-related pollution. Within the dynamic landscape of logistics innovation, various multicriteria decision-making (MCDM) approaches empower businesses to evaluate freight transport options thoroughly. In this study, we utilize a case study to demonstrate the application of the Technique for Order Preference by Similarity to the Ideal Solution (TOPSIS) algorithm for MCDM decision-making in freight mode selection. We further enhance the TOPSIS framework by integrating the entropy weight coefficient method. This enhancement aids in assigning precise weights to each criterion involved in mode selection, leading to a more reliable decision-making process. The proposed model provides cost-effective and timely deliveries, minimizing environmental footprint and meeting consumers’ needs. Our findings reveal that freight carbon footprint is the primary concern, followed by freight cost, time sensitivity, and service reliability. The study identifies the combination of Rail/Truck as the ideal mode of transport and containers in flat cars (COFC) as the next best option for the selected case. The proposed algorithm, incorporating the enhanced TOPSIS framework, benefits companies navigating the complexities of multimodal transport. It empowers making more strategic and informed transportation decisions. This demonstration will be increasingly valuable as companies navigate the ever-growing trade within the global supply chains.

关键词： Multimodal Freight Transport MCDM TOPSIS Entropy Technique COFC

Urban Freight Consolidation Model for Post-Hauler Planning

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Journal of Transportation Technologies 2024年第2期14卷 255-272页

作者： mohammad Anwar Rahman School of Engineering Science & Technology Central Connecticut State University New Britain USA

Freight transportation in urban areas has increased significantly in a shorter period due to the widespread use of e-commerce, fast delivery, and population growth. Recently, a noticeable government initiative aimed at creating an effective, acceptable, and sustainable city logistics policy. This paper examines freight consolidation as a transportation strategy for optimizing last-mile delivery costs. Freight consolidation involves combining smaller shipments from various origins into a single, larger shipment for more efficient transportation to a common destination. This approach is particularly beneficial for last-mile delivery, where frequent deliveries of smaller quantities are frequently visible. Finally, we provide an illustrative example targeting urban freight stakeholders for practicing possible consolidation methodology. The result in the illustrative example shows that freight with 3-day consolidation, despite the delay penalty, is cheaper than daily shipping, and both are cheaper than 2-day consolidated shipping. The study will benefit urban businesses and freight services.

关键词： Freight Consolidation Urban Transport Intermodal Last-Mile Delivery

A review:Health benefits and physicochemical characteristics of blended vegetable oils

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Grain & Oil Science and Technology 2024年第2期7卷 113-123页

作者： Hina Daud Memon Sarfaraz Ahmed Mahesar Sirajuddin Huseyin Kara Syed Tufail Hussain Sherazi mohammad Younis Talpur National Centre of Excellence in Analytical Chemistry University of SindhJamshoro 76080Pakistan International Center for Chemical and Biological Sciences HEJ Research Institute of ChemistryUniversity of KarachiKarachi 75270Pakistan Department of Chemistry Faculty of ScienceSelcuk UniversityKonya 42075Turkey Dr.M.A.Kazi Institute of Chemistry University of SindhJamshoro 76080Pakistan

Oil blending is the method of choice used worldwide to improve oxidative stability and nutritional *** is no such edible oil/fat that meets all the recommendations from the health point of *** fatty acid composition of vegetable oils decides the fate of the *** single oil is unable to provide a balanced amount of fatty acids(FAs)required/recommended on a daily intake *** oils/fats is an appropriate procedure of physically mixing multiple oils in suitable proportions which may provide functional lipids with improved antioxidant potential and desirable physical and chemical *** review piled up the accessible data on the blending of diverse oils/fats in the combination of binary,ternary,quaternary,or other types of oils into a single blended *** can be found very convincing towards appropriate FA profile,enhancement in physicochemical characteristics,and augmented stability for the period of storage or when used as cooking/frying processes which could ultimately serve as an effectual dietary intervention towards the health protectiveness.

关键词： Vegetable oil Oil blending Physicochemical properties Nutritional benefits

Are gender inclusiveness and rural transformation interlinked?The case of Bangladesh

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Journal of Integrative Agriculture 2024年第6期23卷 2112-2126页

作者： Maria Fay Rola-Rubzen mohammad Jahangir Alam Jon Marx Sarmiento Ismat Ara Begum Al Amin Al Abbasi Subrata Saha UWA School of Agriculture and Environment The University of Western AustraliaPerth 6009Australia Department of Agribusiness and Marketing Bangladesh Agricultural UniversityMymensingh-2202Bangladesh Department of Agricultural Economics Bangladesh Agricultural UniversityMymensingh-2202Bangladesh

Gender inclusiveness is important in the rural transformation process of Bangladesh as rural women play key roles in both agricultural and non-agricultural *** inclusiveness also leads to gender *** empirically evaluate relationships between rural transformation and gender inclusiveness in *** consider three rural transformation indicators:high-value commodities’share in agricultural output values,non-farm employment’s share in rural labor employment,and non-agricultural GDP’s share in total *** capturing gender inclusiveness include the per capita rural income of males and females,ratio of investments into gender programs,female access to education,access to healthcare,employment participation,land ownership,and asset *** test the effect of the difference in per capita rural income of males and females and the ratio of their incomes and check for the robustness of the gender variables across different model *** 128 district-level observations from 32 districts of Bangladesh across four time periods(2000,2005,2010 and 2016),we use both ordinary least squares and fixed effects panel regression *** find that female land and asset ownership and access to education and healthcare are robust determinants of various stages of rural ***,our results suggest that improving women’s ownership of land and assets and investing in women’s education and healthcare will likely contribute to a more inclusive rural transformation.

关键词： rural transformation Bangladesh gender inclusiveness investments on women