检索结果-南通市图书馆

Insulin like growth factor-1 increases fatty liver preservation in IGL-1 solution

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2010年第45期16卷 5693-5700页

作者： mohamed amine zaouali Susagna Padrissa-Altés Ismail Ben Mosbah Hassen Ben Abdennebi Olivier Boillot Antoni Rimola Dalila Saidane-Mosbahi Joan Roselló-Catafau Experimental Hepatic Ischemia-Reperfusion Unit Institut d'Investigacions Biomèdiques de BarcelonaConsejo Superior de Investigaciones Científicas08036 BarcelonaSpain Unitat de Transplantament de Fetgei Viabilitat del' Empelt Institut d'Investigacions Biomèdiques August Pii Sunyer08036 BarcelonaSpain Laboratory of Human Physiology Faculty of PharmacyUniversity of Monastir5000 MonastirTunisia Unit of Hepatic Transplantation Edouard Herriot Hospital69003 LyonFrance Biomedical Research Center Esther Koplowitz CIBER-ehdInstitute of Health Carlos Ⅲ08036 BarcelonaSpain

AIM: To investigate the benefits of insulin like growth factor-1 (IGF-1) supplementation to serum-free institut georges lopez-1 (IGL-1) solution to protect fatty liver against cold ischemia reperfusion injury. METHODS: Steatotic livers were preserved for 24 h in IGL-1  solution supplemented with or without IGF-1 and then perfused "ex vivo " for 2 h at 37℃. We examined the effects of IGF-1 on hepatic damage and function (transaminases, percentage of sulfobromophthalein clearance in bile and vascular resistance). We also studied other factors associated with the poor tolerance of fatty livers to cold ischemia reperfusion injury such as mitochondrial damage, oxidative stress, nitric oxide, tumor necrosis factor-α (TNF-α) and mitogen-activated protein ***: Steatotic livers preserved in IGL-1 solutionsupplemented with IGF-1 showed lower transaminase levels, increased bile clearance and a reduction in vascular resistance when compared to those preserved in IGL-1solution alone. These benefits are mediated by activation of AKT and constitutive endothelial nitric oxide synthase (eNOS), as well as the inhibition of inflammatory cytokines such as TNF-α. Mitochondrial damage and oxidative stress were also ***: IGL-1  enrichment with IGF-1 increasedfatty liver graft preservation through AKT and eNOS activation, and prevented TNF-α release during normothermic reperfusion.

关键词： AKT Institut georges lopez-1 solution Insulin like growth factor-1 Ischemia reperfusion injury Nitric oxide Oxidative stress Steatotic graft preservation

Relevance of proteolysis and proteasome activation in fatty liver graft preservation: An Institut Georges Lopez-1 vs University of Wisconsin appraisal

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2017年第23期23卷 4211-4221页

作者： mohamed amine zaouali Arnau Panisello-Roselló Alexandre Lopez Carlos Castro Benítez Emma Folch-Puy Agustín García-Gil Teresa Carbonell RenéAdam Joan Roselló-Catafau Experimental Hepatic Ischemia-Reperfusion Unit Institut d’Investigacions Biomèdiques de BarcelonaSpanish National Research Council Research Unit of Biology and molecular anthropology applied to development and Health(UR12ES11) Faculty of PharmacyUniversity of Monastir Tunisia and Institute of Biotechnology of Monastir University of Monastir Centre Hépatobiliaire AP-PHHopital Paul Brousse Hospital Clínico de Zaragoza Facultat Biologia Universitat de Barcelona

To compare liver proteolysis and proteasome activation in steatotic liver grafts conserved in University of Wisconsin (UW) and Institut Georges Lopez-1 (IGL-1) *** liver grafts from male obese Zücker rats were conserved in UW and IGL-1 solutions for 24 h at 4 °Cand subjected to “ex vivo” normo-thermic perfusion (2 h; 37 °C). Liver proteolysis in tissue specimens and perfusate was measured by reverse-phase high performance liquid chromatography. Total free amino acid release was correlated with the activation of the ubiquitin proteasome system (UPS: measured as chymotryptic-like activity and 20S and 19S proteasome), the prevention of liver injury (transaminases), mitochondrial injury (confocal microscopy) and inflammation markers (TNF 1 alpha, high mobility group box-1 (HGMB-1) and PPAR gamma), and liver apoptosis (TUNEL assay, cytochrome c and caspase 3).RESULTSProfiles of free AA (alanine, proline, leucine, isoleucine, methionine, lysine, ornithine, and threonine, among others) were similar for tissue and reperfusion effluent. In all cases, the IGL-1 solution showed a significantly higher prevention of proteolysis than UW (P < 0.05) after cold ischemia reperfusion. Livers conserved in IGL-1 presented more effective prevention of ATP-breakdown and more inhibition of UPS activity (measured as chymotryptic-like activity). In addition, the prevention of liver proteolysis and UPS activation correlated with the prevention of liver injury (AST/ALT) and mitochondrial damage (revealed by confocal microscopy findings) as well as with the prevention of inflammatory markers (TNF1alpha and HMGB) after reperfusion. In addition, the liver grafts preserved in IGL-1 showed a significant decrease in liver apoptosis, as shown by TUNEL assay and the reduction of cytochrome c, caspase 3 and P62 *** comparison of these two preservation solutions suggests that IGL-1 helps to prevent ATP breakdown more effectively than UW and subsequently achieves a hig

关键词： Liver proteolysis Proteasome activation Fatty liver preservation Institut Georges Lopez-1 University of Wisconsin High mobility group box 1 Cold ischemia reperfusion injury

Role of sirtuins in ischemia-reperfusion injury

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2013年第43期19卷 7594-7602页

作者： Eirini Pantazi mohamed amine zaouali mohamed Bejaoui Emma Folch-Puy Hassen Ben Abdennebi Joan Roselló-Catafau Experimental Hepatic Ischemia-Reperfusion Unit Institut of Biomedical Research of Barcelona-Spanish National Research CouncilBarcelona08036 CataloniaSpain Molecular Biology and Anthropology applied to development and health (UR12ES11) Faculty of PharmacyMonastir 5000Tunisia Experimental Pathology Department Institute of Biomedical Research of Barcelona-Spanish National Research CouncilBarcelona08036 CataloniaSpain

Ischemia-reperfusion injury(IRI)remains an unresolved and complicated situation in clinical practice,especially in the case of organ *** factors contribute to its complexity;the depletion of energy during ischemia and the induction of oxidative stress during reperfusion initiate a cascade of pathways that lead to cell death and finally to severe organ ***,the sirtuin family of nicotinamide adenine dinucleotide-dependent deacetylases has gained increasing attention from researchers,due to their involvement in the modulation of a wide variety of cellular *** are seven mammalian sirtuins and,among them,the nuclear/cytoplasmic sirtuin 1(SIRT1)and the mitochondrial sirtuin 3(SIRT3)are ubiquitously expressed in many tissue *** are known to play major roles in protecting against cellular stress and in controlling metabolic pathways,which are key processes during *** this review,we mainly focus on SIRT1 and SIRT3 and examine their role in modulating pathways against energy depletion during ischemia and their involvement in oxidative stress,apoptosis,microcirculatory stress and inflammation during *** present evidence of the beneficial effects of sirtuins against IRI and emphasize the importance of developing new strategies by enhancing their action.

关键词： Sirtuin 1 Sirtuin 3 Ischemia-reperfusion injury oxidative stress Apoptosis

Hypoxia inducible factor-1αaccumulation in steatotic liver preservation:Role of nitric oxide

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2010年第28期16卷 3499-3509页

作者： mohamed amine zaouali Ismail Ben Mosbah Eleonora Boncompagni Hassen Ben Abdennebi Maria Teresa Mitjavila Ramon Bartrons Isabel Freitas Antoni Rimola Joan Roselló-Catafau Experimental Hepatic Ischemia Reperfusion Unit Institut of Biomedical Research of BarcelonaSpanish National Research Council08036BarcelonaSpain Liver Transplantation and Graft Viability Unit August Pi i Sunyer Biomedical Research Institut08036Barcelona Spain Animal Biology Department Pavia University27100PaviaItaly Laboratory of Human Physiology Faculty of PharmacyUniversity of Monastir5000Monastir Tunisia Department of Physiology Faculty of BiologyUniversity of Barcelona08028BarcelonaSpain Physiological Sciences Department Bellvitge CampusInstitut of Biomedical Research of Bellvitge-Barcelona University08907BarcelonaSpain

AIM:To examine the relevance of hypoxia inducible factor(HIF-1)and nitric oxide(NO)on the preservation of fatty liver against cold ischemia-reperfusion injury(IRI). METHODS:We used an isolated perfused rat liver model and we evaluated HIF-1αin steatotic and non-steatotic livers preserved for 24 h at 4℃in University of Wisconsin and IGL-1 solutions,and then subjected to 2 h of normothermic *** normoxic reperfusion,liver enzymes,bile production,bromosulfophthalein clearance,as well as HIF-1αand NO[endothelial NO synthase(eNOS)activity and nitrites/nitrates]were also *** factors associated with the higher susceptibility of steatotic livers to IRI,such as mitochondrial damage and vascular resistance were evaluated. RESULTS:A significant increase in HIF-1αwas found in steatotic and non-steatotic livers preserved in IGL-1 after cold *** preserved in IGL-1 showed a significant attenuation of liver injury and improvement in liver function *** benefits were enhanced by the addition of trimetazidine(an antiischemic drug),which induces NO and eNOS activation, to IGL-1 *** normoxic reperfusion,the presence of NO favors HIF-1αaccumulation,promoting also the activation of other cytoprotective genes,such as hemeoxygenase-1. CONCLUSION:We found evidence for the role of the HIF-1α/NO system in fatty liver preservation,especially when IGL-1 solution is used.

关键词： Fatty liver Tissue preservation Hypoxia inducible factor-1α IGL-1 Nitric oxide Trimetazidine

Losartan activates sirtuin 1 in rat reduced-size orthotopic liver transplantation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2015年第26期21卷 8021-8031页

作者： Eirini Pantazi mohamed Bejaoui mohamed amine zaouali Emma Folch-Puy Anabela Pinto Rolo Arnau Panisello Carlos Marques Palmeira Joan Roselló-Catafau Experimental Pathology Department Experimental Hepatic Ischemia-Reperfusion UnitInstitute of Biomedical Research of Barcelona(IIBB-CSIC Department of Life Sciences Faculty of Science and TechnologyUniversity of Coimbra Portugal and Center for Neurosciences and Cell Biology University of Coimbra

AIM: To investigate a possible association between losartan and sirtuin 1(SIRT1) in reduced-size orthotopic liver transplantation(ROLT) in ***: Livers of male Sprague-Dawley rats(200-250 g) were preserved in University of Wisconsin preservation solution for 1 h at 4 ℃ prior to *** an additional group,an antagonist of angiotensin Ⅱ type 1 receptor(AT1R),losartan,was orally administered(5 mg/kg) 24 h and 1 h before the surgical procedure to both the donors and the ***(as an indicator of liver injury),SIRT1 activity,and nicotinamide adenine dinucleotide(NAD+,a co-factor necessary for SIRT1 activity) levels were determined by biochemical *** expression of SIRT1,acetylated Fox O1(ac-Fox O1),NAMPT(the precursor of NAD+),heat shock proteins(HSP70,HO-1) expression,endoplasmic reticulum stress(GRP78,IRE1 a,p-e IF2) and apoptosis(caspase 12 and caspase 3) parameters were determined by Western *** alterations in protein expression of mitogen activated protein kinases(MAPK),such as p-p38 and p-ERK,were also ***,the SIRT3 protein expression and m RNA levels were ***: The present study demonstrated that losartan administration led to diminished liver injury when compared to ROLT group,as evidenced by the significant decreases in alanine aminotransferase(358.3 ± 133.44 vs 206 ± 33.61,P < 0.05) and aspartate aminotransferase levels(893.57 ± 397.69 vs 500.85 ± 118.07,P < 0.05).The lessened hepatic injury in case of losartan was associated with enhanced SIRT1 protein expression and activity(5.27 ± 0.32 vs 6.08 ± 0.30,P < 0.05).This was concomitant with increased levels of NAD+(0.87 ± 0.22 vs 1.195 ± 0.144,P < 0.05) the co-factor necessary for SIRT1 activity,as well as with decreases in ac-Fox O1 *** treatment also provoked significant attenuation of endoplasmic reticulum stress parameters(GRP78,IRE1 a,p-e IF2) which was consistent with reduced levels of both caspase 12 and caspase 3.

关键词： Losartan Sirtuin 1 Endoplasmic reticulumstress Liver ischemia reperfusion injury Angiotensin Ⅱ

Security and Privacy in Solar Insecticidal Lamps Internet of Things:Requirements and Challenges

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

IEEE/CAA Journal of Automatica Sinica 2024年第1期11卷 58-73页

作者： Qingsong Zhao Lei Shu Kailiang Li mohamed amine Ferrag Ximeng Liu Yanbin Li the College of Artificial Intelligence Nanjing Agricultural UniversityNanjing 210031 the State Key Laboratory for Novel Software Technology Nanjing UniversityNanjing 210023 the State Key Laboratory of Cryptology Beijing 100878China IEEE the School of Engineering University of LincolnLincoln LN67TSUK the Artificial Intelligence and Digital Science Research Center Technology Innovation InstituteMasdar City 9639UAE the College of Computer and Data Science Fuzhou UniversityFuzhou 350108China

Solar insecticidal lamps(SIL) can effectively control pests and reduce the use of pesticides. Combining SIL and Internet of Things(IoT) has formed a new type of agricultural IoT,known as SIL-IoT, which can improve the effectiveness of migratory phototropic pest control. However, since the SIL is connected to the Internet, it is vulnerable to various security *** issues can lead to serious consequences, such as tampering with the parameters of SIL, illegally starting and stopping SIL,etc. In this paper, we describe the overall security requirements of SIL-IoT and present an extensive survey of security and privacy solutions for SIL-IoT. We investigate the background and logical architecture of SIL-IoT, discuss SIL-IoT security scenarios, and analyze potential attacks. Starting from the security requirements of SIL-IoT we divide them into six categories, namely privacy, authentication, confidentiality, access control, availability,and integrity. Next, we describe the SIL-IoT privacy and security solutions, as well as the blockchain-based solutions. Based on the current survey, we finally discuss the challenges and future research directions of SIL-IoT.

关键词： Challenges Internet of Things(IoT) privacy and security security requirements solar insecticidal lamps(SIL)

Multi-task learning for PBFT optimisation in permissioned blockchains

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Blockchain(Research and Applications) 2024年第3期5卷 89-105页

作者： Kenza Riahi mohamed-el-amine Brahmia Abdelhafid Abouaissa Lhassane Idoumghar CESI LINEACT UR 7527StrasbourgFrance IRIMAS University of Haute AlsaceUR 7499MulhouseFrance

Finance,supply chains,healthcare,and energy have an increasing demand for secure transactions and data *** blockchains fulfilled this need thanks to the consensus protocol that ensures that participants agree on a common *** of the most widely used protocols in private blockchains is the Practical Byzantine Fault Tolerance(PBFT),which tolerates up to one-third of Byzantine nodes,performs within partially synchronous systems,and has superior throughput compared to other *** has,however,an important bandwidth consumption:2N(N-1)messages are exchanged in a system composed of𝑁nodes to validate only one *** is possible to reduce the number of consensus participants by restricting the validation process to nodes that have demonstrated high levels of security,rapidity,and *** this paper,we propose the first database that traces the behavior of nodes within a system that performs PBFT *** reflects their level of security,rapidity,and availability throughout the *** first investigate different Single-Task Learning(STL)techniques to classify the nodes within our ***,using Multi-Task Learning(MTL)techniques,the results are much more interesting,with classification accuracies over 98%.Integrating node classification as a preliminary step to the PBFT protocol optimizes the *** the best cases,it is able to reduce the latency by up to 94%and the communication traffic by up to 99%.

关键词： Blockchain PBFT Dataset Nodes classification Single-task learning Multi-task learning

Sirtuin 1 in rat orthotopic liver transplantation:An I GL-1 preservation solution approach

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2015年第6期21卷 1765-1774页

作者： Eirini Pantazi mohamed amine zaouali mohamed Bejaoui Emma Folch-Puy Hassen Ben Abdennebi Ana Teresa Varela Anabela Pinto Rolo Carlos Marques Palmeira Joan Roselló-Catafau Experimental Hepatic Ischemia Reperfusion Unit Institute of Biomedical Research of Barcelona Faculty of Pharmacy Molecular Biology and Anthropology Applied to Development and Health Department of Life Sciences and Center for Neuroscience and Cell Biology University of Coimbra Department of Biology University of Aveiro

AIM:To investigate the possible involvement of Sirtuin1(SIRT1)in rat orthotopic liver transplantation(OLT),when Institute Georges Lopez 1(IGL-1)preservation solution is enriched with trimetazidine(TMZ).METHODS:Male Sprague-Dawley rats were used as donors and *** were stored in IGL-1 preservation solution for 8h at 4℃,and then underwent OLT according to Kamada’s cuff technique without *** another group,livers were stored in IGL-1 preservation solution supplemented with TMZ,at10-6 mol/L,for 8 h at 4℃and then underwent *** were sacrificed 24 h after reperfusion,and liver and plasma samples were *** injury(transaminase levels),mitochondrial damage(glutamate dehydrogenase activity)oxidative stress(malondialdehyde levels),and nicotinamide adenine dinucleotide(NAD+),the cofactor necessary for SIRT1 activity,were determined by biochemical ***1 and its substrates(acFox O1,ac-p53),the precursor of NAD+,nicotinamide phosphoribosyltransferase(NAMPT),as well as the phosphorylation of adenosine monophosphate activated protein kinase(AMPK),p-m TOR,p-p70S6K(direct substrate of m TOR),autophagy parameters(beclin-1,LC3B)and MAP kinases(p-p38 and p-ERK)were determined by Western ***:Liver grafts preserved in IGL-1 solution enriched with TMZ presented reduced liver injury and mitochondrial damage compared with those preservedin IGL-1 solution *** addition,livers preserved in IGL-1+TMZ presented reduced levels of oxidative *** was consistent with enhanced SIRT1 protein expression and elevated SIRT1 activity,as indicated by decreased acetylation of p53 and Fox *** elevated SIRT1 activity in presence of TMZ can be attributed to the enhanced NAMPT protein and NAD+/NADH ***-regulation of SIRT1 was consistent with activation of AMPK and inhibition of phosphorylation of m TOR and its direct substrate(p-p70S6K).As a consequence,autophagy mediators(beclin-1 and LC3B)were ***,MAP kinases w

关键词： Sirtuin 1 Ischemia-reperfusion injury Liver transp

Does wet-cupping therapy improve repeated sprint ability,perceived wellness,and rating of perceived exertion in young active males?

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Sports Medicine and Health Science 2024年第4期6卷 378-384页

作者： Ismail Dergaa amine Ghram mohamed Romdhani amine Souissi Achraf Ammar Ramadan Abdelmoez Farahat mohamed Saifeddin Fessi Khadijeh Irandoust Morteza Taheri Tasnim Masmoudi mohamed amine Dergaa Nizar Souissi Omar Hammouda Katja Weiss Karim Chamari Helmi Ben Saad Beat Knechtle Primary Health Care Corporation(PHCC) DohaP.O.Box 26555Qatar Research Unit Physical Activity Sportand HealthUR18JS01National Observatory of SportTunis 1003Tunisia High Institute of Sport and Physical Education University of SfaxSfaxTunisia Department of Cardiac Rehabilitation Heart HospitalHamad Medical CorporationDohaQatar Universite de Sousse Faculte de Medecine de SousseHopital Farhat HACHEDLaboratoire de Recherche(Insuffisance CardiaqueLR12SP09)SousseTunisia Healthy Living for Pandemic Event Protection(HL-PIVOT)Network ChicagoILUSA Interdisciplinary Laboratory in Neurosciences Physiology and Psychology:Physical ActivityHealth and LearningUFR STAPSUPLParis Nanterre UniversityNanterreFrance Department of Training and Movement Science Institute of Sport ScienceJohannes Gutenberg-University Mainz55128 MainzGermany Research Laboratory Molecular Bases of Human PathologyLR19ES13Faculty of Medicine of SfaxUniversity of SfaxSfax 3029Tunisia Faculty of Medicine Kafrelsheikh UniversityKafrelsheikh33511Egypt Department of Behavioral and Cognitive Sciences in Sport Faculty of Sport Sciences and HealthUniversity of TehranIran University of Sousse Farhat HACHED HospitalForensic ServiceSousseTunisia Medbase St.Gallen Am Vadianplatz St.GallenSwitzerland Aspetar Orthopaedic and Sports Medicine HospitalFIFA Medical Centre of ExcellenceDohaP.O.Box 29222Qatar Institute of Primary Care University of ZurichZurichSwitzerland

Wet-cupping therapy(WCT)is one of the oldest known medical techniques,used as a traditional and complementary therapy with a wide application all around the world for general *** on the effects of WCT on sports performance are sparse and ***,we aimed to explore the effects of WCT on repeated sprint ability,wellness,and exertion in young active ***-nine active adult males(age:[28±5]years;body height[177±8]cm;body mass:[79±7]kg;body mass index:[25.4±1.8]kg/m^(2))were selected for the *** participants performed a running-based sprint test on two separate occasions(Control and Post-WCT).WCT was performed 24 h before the testing *** completed the Hooper questionnaire to assess their well-being(i.e.,sleep,stress,fatigue,and soreness)before each *** rating of perceived exertion(RPE)was collected after each testing session.A higher maximum power(p<0.05,effect size[ES]=0.6),mean power(p<0.01,ES=0.5)and minimum power(p<0.01,ES=0.6)were recorded post-WCT as compared to Control session along with a better perceived sleep(p<0.01,ES=0.85).Perceived stress(p<0.01,ES=0.6)and RPE(p<0.001;ES=1.1)were lower during the post-WCT compared to the Control *** present findings demonstrated that WCT moderately enhanced repeated sprint ability and had positive effects on perceived sleep,stress,and *** may be an effective ergogenic aid to improve repeated sprint ability and general wellbeing in young adult *** large-scale multicentric clinical studies are paramount to confirm the results of our study.

关键词： Alternative medicine Complementary medicine Hijama Short-term maximal performance Sports medicine