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Characterization of root-nodulating bacteria on Retama raetam in arid Tunisian soils

Progress in Natural Science:Materials International 2008年第1期18卷 43-49页

作者： Mosbah Mahdhi Angèle Nzoué Philippe de Lajudie mohamed mars Laboratory of Plant Biotechnology Applied to Crop Improvement Faculty of Sciences of Gabès Erriadh City Zrig 6072 Gabès Tunisia Laboratory of Tropical and Mediterranean Symbioses IRD/INRA/CIRAD/UM2/AGRO-M (UMR 113) Montpellier F34398 France

The aim of this study is to investigate the diversity of Retama raetam root-nodule bacteria isolated from arid regions of Tunisia. Twelve isolates, chosen as representative for difierent 16S rRNA gene patterns, were characterized by 16S rRNA gene sequencing and phenotypic analysis. Isolates were assigned to Sinorhizobium, Rhizobium and Agrobacterium. Symbiotic properties of Sinorhizobium and Rhizobium isolates showed a large diversity in their capacity to infect their host plant and fix atmospheric nitrogen. Strain RK 22 identified as Rhizobium was the most effective isolate.

关键词： Retama raetam Rhizobia 16S rRNA gene sequence Tunisia

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Genetic diversity of rhizobial populations recovered from three Lotus species cultivated in the infra-arid Tunisian soils

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Progress in Natural Science:Materials International 2009年第9期19卷 1079-1087页

作者： Mokhtar Rejili Maria Josè Lorite Mosbah Mahdhi Juan Sanjuan Pinill Ali Ferchichi mohamed mars Arid & Oases Cropping Laboratory Arid Area Institute (IRA) Laboratory of Plant Biotechnology Applied to Crop Improvement Faculty of Sciences of Gabès Soil Microbiology & Symbiotic Systems Department Experimental Station of Zaidìn

Eighty-three bacterial strains isolated from root nodules of Lotus creticus, L. pusillus, and L. arabicus grown in infra-arid Tunisian soils were characterized using a polyphasic approach including phenotypic analysis, rep-PCR and PCR-RFLP analyses of the 16S rRNA gene. Phenotypically, all isolates are fast growers the majority of which grow at a pH of between 5.5 and 9. Most of the tested isolates tolerate NaCl concentrations from 1.39% to 3.48%. By rep-PCR fingerprinting, the genomic similarity varied from 30% to 98%. All tested isolates were clustered into 32 rep-PCR clusters at the similarity level of 80%. The genomic divergence of strains revealed by rep/PCR analysis appeared to be very important since a molecular polymorphism delimiting symbionts for each species of Lotus was identified. With the high-resolution of rep-PCR profiles of the isolates obtained using Pearson’s/UPGMA analysis, the isolates were resolved into 60 different profiling groups to undergo 16S ARDRA analyses. The analysis of all restriction fragments from each strain based on the UPGMA algorithm from the combined patterns showed that Lotus isolates are very diverse and that they were affiliated to Sinorhizobium, Rhizobium, and Mesorhizobium genera.

关键词： Lotus sp. rep-PCR fingerprinting PCR-RFLP 16S rRNA Rhizobia Arid habitat

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Analysis of Genetic Diversity and Relationships in a Tunisian Fig （Ficus carica） Germplasm Collection by Random Amplified Microsatellite Polymorphisms

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Journal of Integrative Plant Biology 2007年第3期49卷 386-391页

作者： Khaled Chatti Olfa Saddoud Amel Salhi-Hannachi Messaoud mars mohamed Marrakchi Mokhtar Trifi Laboratory of Molecular Genetics Immunology and Biotechnology Faculty of Sciences Campus University 2092 EI Manar Tunis Tunisia High Institute of Breeding and Horticulture 4042 Chott Mariem Sousse Tunisia

The random amplified mirosatellite polymorphism method was performed in a set of Tunisian fig landraces using eighteen primer combinations. A total of sixty three random amplified microsatellite polymorphism （RAMPO） markers were scored and used either to assess the genetic diversity in these cultivars or to detect cases of mislabeling. Opportunely, data proved that the designed procedure constitutes an attractive and fast method with low costs and prevents radio exposure. As a result, we have identified the primer combinations that are the most efficient to detect genetic polymorphism in this crop. Therefore, the derived unweighted pair-group method with arithmetic averages （UPGMA） dendrogram illustrates the genetic divergence among the landraces studied and exhibits a typically continuous variation. Moreover, no evident correlation between the sexes of trees was observed. In addition, using these markers, discrimination between landraces has been achieved. Thus, random amplified mirosatellite polymor- phism is proved to be powerful for characterizing the local fig germplasm.

关键词： collection Ficus carica genetic diversity germplasm random amplified microsatellite polymorphism Tunisia

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Landslide susceptibility mapping using machine learning algorithms and comparison of their performance at Abha Basin,Asir Region,Saudi Arabia

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Geoscience Frontiers 2021年第2期12卷 639-655页

作者： Ahmed mohamed Youssef Hamid Reza Pourghasemi Geology Department Faculty of ScienceSohag UniversityEgypt Geological Hazards Department Applied Geology SectorSaudi Geological SurveyP.O.Box 54141Jeddah21514Saudi Arabia Department of Natural Resources and Environmental Engineering College of AgricultureShiraz UniversityShirazIran

The current study aimed at evaluating the capabilities of seven advanced machine learning techniques(MLTs),including,Support Vector Machine(SVM),Random Forest(RF),Multivariate Adaptive Regression Spline(mars),Artificial Neural Network(ANN),Quadratic Discriminant Analysis(QDA),Linear Discriminant Analysis(LDA),and Naive Bayes(NB),for landslide susceptibility modeling and comparison of their *** machine learning algorithms with spatial data types for landslide susceptibility mapping is a vitally important *** study was carried out using GIS and R open source software at Abha Basin,Asir Region,Saudi ***,a total of 243 landslide locations were identified at Abha Basin to prepare the landslide inventory map using different data *** the landslide areas were randomly separated into two groups with a ratio of 70%for training and 30%for validating *** landslide-variables were generated for landslide susceptibility modeling,which include altitude,lithology,distance to faults,normalized difference vegetation index(NDVI),landuse/landcover(LULC),distance to roads,slope angle,distance to streams,profile curvature,plan curvature,slope length(LS),and *** area under curve(AUC-ROC)approach has been applied to evaluate,validate,and compare the MLTs *** results indicated that AUC values for seven MLTs range from 89.0%for QDA to 95.1%for *** findings showed that the RF(AUC=95.1%)and LDA(AUC=941.7%)have produced the best performances in comparison to other *** outcome of this study and the landslide susceptibility maps would be useful for environmental protection.

关键词： Landslide susceptibility Machine learning algorithms Variables importance Saudi Arabia

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Rapid Prototyping and Validation of mars 0913 Brushless Motor to Develop Mild HEV

Rapid Prototyping and Validation of Mars 0913 Brushless Moto...

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2015中国汽车工程学会年会

作者： mohamed Awadallah Peter Tawadros Nong Zhang University of Technology Sydney

We discuss a mild hybrid powertrain based on a solution placing a small electric motor behind the first reduction *** electric motor may be used for power supplementation,and is controlled in both power and *** the primary use of the motor is for torque-hole fill-in in an AMT-equipped *** motor is controlled to reduce clutch slip,and provide torque fill-in during gear *** paper will describe the real-time control and the validation of mars 0913 BLDC *** validation process involves specific implementation techniques for model processing and interfaces in real-time with electrical hardware *** tools and interfaces necessary to that validation are also presented,and include KHB 12601 BLDC Kelly Controller,dSPACE MicroAutoBox II,for hybrid vehicle *** will include a design processes for handling real-time control using Simulink,DSPACE RTI＆Control Desk environment and finally,the validation based on Dynamometer testing to get all performance data of BLDC motor and mechanical parts as designed by *** paper is based on the technique of Rapid Controller Prototyping（RCP）.It is an efficient method for solving motor control problems such as *** this design,we connect the universal controller（MicroAutoBox）to the KHB *** interface signals are configured and then the two controllers are connected by Relays and CAN line according to the specifications of the real system.

关键词： automotive applications brushless motors control design hybrid systems motor drives permanent magnet motors

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