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Fermentation, formulation and evaluation of PGPR Bacillus subtilis isolate as a bioagent for reducing occurrence of peanut soil-borne diseases

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Journal of Integrative Agriculture 2019年第9期18卷 2080-2092页

作者： abdel-Gayed M.Ahmad Abo-Zaid G.Attia Matar S.mohamed hafez E.Elsayed Onion Garlic and Oil Crops Research DepartmentPlant Pathology Research InstituteAgricultural Research CenterGiza 12619Egypt Bioprocess Development Department Genetic Engineering and Biotechnology Research InstituteCity of Scientific Research and Technological ApplicationsNew Borg El-Arab CityAlexandria 21934Egypt Chemical Engineering Department Faculty of EngineeringJazan UniversityJazan 45142Saudi Arabia Plant Protection and Biomolecular Diagnosis Department Arid Lands Cultivation Research InstituteCity of Scientific Research and Technological ApplicationsNew Borg El-Arab CityAlexandria 21934Egypt

Four isolates of Bacillus subtilis coded,B4,B7,B8 and B10 were examined as biocontrol agents for their abilities and antagonistic effect on the in vitro growth of certain phytopathogenic fungi of peanut,Rhizoctonia solani and Sclerotium *** subtilis isolate B4(GenBank accession ***150884)was the highly effective one for inhibiting the fungal mycelial *** fermentation of *** isolate B4 was carried out and the maximum biomass achieved was 4.53 g L-1 at 11 *** subtilis isolate B4 was formulated and evaluated as a biofungicide to reduce peanut soil-borne diseases under greenhouse and field conditions at the side of Rizolex-T(fungicide)as *** by formulated plant growth-promoting rhizobacteria(PGPR)*** B4 and Rizolex-T in a soil infested with ***,*** and mixture of them were more effective in decreasing percentage of damping-off,root and pod rot disease incidence(%)in greenhouse and open field environment during the two seasons 2015 and *** by PGPR gave highly dry weight and number of healthy pods compared to control of fungi treatment which was nearby to dry weights of healthy pods achieved by treatments by Rizolex-T in a soil infested with ***,*** and mixture of *** PGPR *** B4 gave higher increasing of yield percentage than treatment by Rizolex-T in the two evaluated seasons 2015 and *** can conclude that the produced bioforumlated agent was more efficient as fungicide when compared with the other chemical synthesized fungicides,safe for human and the environment and economy.

关键词： peanut soil-borne diseases Bacillus subtilis biocontrol fermentation formulation

Accuracy of Sentinel Lymph Node Biopsy after Neoadjuvant Chemotherapy in Breast Cancer Patients;Single Center Experience

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Surgical Science 2018年第1期9卷 52-61页

作者： Waleed Elnahas Omar Hamdy Khaled abdel Wahab Sameh Roshdy Sara Raafat mohamed hafez Omar Farouk mohamed Elmetwally Surgical Oncology Unit Mansoura Oncology Centre Mansoura University Mansoura Egypt Pathology Department Faculty of Medicine Mansoura University Mansoura Egypt

Purpose: Most important factor affecting prognosis of breast cancer is axillary nodal involvement. Several studies evaluated the accuracy of Sentinel lymph node biopsy in breast cancer patients post neoadjuvant chemotherapy. In this study, we will examine accuracy and feasibility of using Sentinel lymph node biopsy in predicting axillary lymph node status in breast cancer patients after neoadjuvant chemotherapy. Methods: 45 female patients with resectable, nonmetastatic breast carcinoma cases who received neoadjuvant chemotherapy were enrolled in this study according to the routine Mansoura Oncology Center—guidelines of management of breast cancer. Methylene blue dye used for detection of Sentinel lymph node. Results: Successful Sentinel lymph node detection was 82.2%. Skin involvement (T4 disease) were linked to a low identification (P = 0.005). False negative rate equals 11/27 = (40.7%).With advancement of the stage of the tumor, the incidence of false negative results increases significantly (p = 0.012) with 95% confidence interval;1.2 - 5.4. Conclusion: Sentinel lymph node should be adopted to be the standard method for axillary staging with T1-3 tumors after receiving neoadjuvant chemotherapy, in T4 patients, it is associated with low detection rate & high false negative rate making it doubtful technique for axillary staging.

关键词： Breast Cancer Sentinel Lymph Node Neo Adjuvant Chemotherapy Blue Dye

Effect of Using Baker＇s Yeast and Exogenous Digestive Enzymes as Growth Promoters on Growth, Feed Utilization and Hematological Indices of Nile tilapia, Oreochromis niloticus Fingerlings

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Journal of Agricultural Science and Technology(B) 2012年第1期2卷 15-28页

作者： Ashraf mohamed abdelsamee Goda hafez abdel-Hamid Hassan Mabrouk mohamed Abd EI-Hamid Wafa Tarek mohamed E1-Afifi National Institute of Oceanography and Fisheries （NIOF） Fish Nutrition Laboratory Cairo 24135 Egypt Regional Center for Foods and feeds Agriculture Research Center Ministry of Agriculture Cairo 24135 Egypt

The present study was conducted to evaluate the effect of baker yeast, Saccharomyces cerevisiae (SC) and exogenous digestive enzymes (pepsin, papain and α-amylase, EDE) dietary supplementation on growth performance, feed utilization and hematological indices of Nile tilapia, Oreochromis niloticus fingerlings. A total of 630 Nile tilapia fingerlings with an average body weight of 26.4 ± 0.2 g were divided in the seven experimental net-pen treatments (three replicates each). The experiment was conducted for 119 days. Seven isonitrogenous (26.50%) digestible protein and isocaloric (13.40 MJ kg-1) digestible energy experimental diets were formulated. The control diet had no SC and EDE added. Diets 2-3 each contained SC at levels of 2 and 4 g 100 g diet-1, respectively, while diets 4-5 each contained EDE at levels of (0.64, 1.28, 0.16) and (1.28, 2.56, 0.32) g 100 g diet-1 of pepsin, papain and α-amylase, respectively. Diet 6 contained mixture of SC and EDE at levels of 1 g yeast and 0.32, 0.64, 0.08 g of pepsin, papain and α-amylase, respectively 100 g diet-1 and diet D7 contained 2 g yeast and 0.64, 1.28, 0.16 g of pepsin, papain and α-amylase, respectively 100 g diet-1. Growth performance and feed utilization efficiency of Nile tilapia were significantly (P ? 0.05) higher in all treatments receiving SC and/or EDE supplemented-diets than the control diet which suggests that the addition of SC and EDE enhanced the growth performance. Red blood cells counts, hematocrit and hemoglobin were significantly (P ? 0.05) highest in all treatments receiving mixture of SC and EDE supplemented-diets (D6 + D7). The same trend was observed for total plasma protein and total plasma globulin levels. The results of present study suggested that Nile tilapia fingerlings fed diets containing the mixture of 1 g yeast, SC and 0.32, 0.64, 0.08 g of pepsin, papain and α-amylase, respectively 100 g diet-1, for 119 days had enhanced growth performance, diet utilization efficiency and hematologic

关键词：尼罗罗非鱼饲料利用率面包酵母血液学指标生长促进剂消化酶鱼种 a-淀粉酶

Postoperative Mechanical Axis Alignment and Components Position after Conventional and Patient-Specific Total Knee Arthroplasty

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Open Journal of Orthopedics 2016年第8期6卷 253-258页

作者： mohamed Mosa mohamed Maher A. El Assal Ahmed M. abdel Aal Yaser E. Khalifa Mahmoud A. hafez Orthopaedic Department Alazhar University (Assiut Branch) Assiut Egypt Orthopaedic Department Assiut University Assiut Egypt Orthopaedic Department October 6 University Cairo Egypt

Introduction: Accurate postoperative alignment and implant positioning are determinant factors for successful total knee arthroplasty (TKA). Patient-specific template (PST) is a technique that uses computer technology for the planning, deigning and production of cutting guides. This study aims to compare PST to conventional technique in terms of mechanical axis alignment and component positioning. Patients and method: 109 TKA were performed for 78 patients in 2 groups. Group A included 69 conventional TKA in 55 patients and Group B included 40 patient-specific TKA in 23 patient. Postoperative long-film X-rays were done for all patients to observe the mechanical axis, anatomical axis, lateral distal femoral mechanical angle and medial proximal tibial angle. Results: No statistically significant difference was found between the two groups regarding alignment or component positioning. Conclusion: Both techniques have shown similar results in restoring the mechanical axis and alignment after TKA. However, PST had the advantages of reduced blood loss and shorter operative time.

关键词： Total Knee Arthroplasty Patient-Specific Templates Conventional TKA Mechanical Axis Limb Alignment Component Position

Evaluation of Surgical Outcome after Resection of Pancreatic Tumors, Our Experience in Mansoura Oncology Center, a Middle-Volume Center in Egypt

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Journal of Cancer Therapy 2012年第5期3卷 477-481页

作者： Nazem Shams Mahmoud Mosbah Mohammad Hegazy Osama Hussein Omar Farouk Sameh Roshdy mohamed Tarek hafez Ashraf Khater Khaled abdel Wahab Oncology Center Mansoura University Mansoura Egypt

Background: Pancreatic cancer is the fourth most common cause of cancer related deaths in the world. Surgical resection remains the only potentially curative treatment for adenocarcinoma of the pancreas;only 10% - 20% of patients are candidate for standard pancreatic resection. Objective: To evaluate perioperative mortality, morbidity and survival for patients underwent PD in middle-volume center. Patients and Methods: Sixty patients with pancreatic tumors were enrolled in this study for different surgical procedure according to type of tumors. Results: No early postoperative complications were observed in 60% of patients, whereas 40% of patients developed one or more complication, the median survival for this group was 12.3 months with standard deviation 3.8 months.

关键词： Pancreatic Cancer Whipple Complication Survival

Immune Response after Hepatitis B Vaccination among Egyptian Medical Students in Nile Delta

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World Journal of Vaccines 2015年第3期5卷 140-146页

作者： Reem Elmaghlob Gamal El Didamony Ashraf Elbahrawy Abdallah Mahmoud Abdallah Mahmoud Haddad Hemida Ahmed Elwassief mohamed Salah Ali Ahmed Al-Ashker abdel-Gawad Saied Mohammad mohamed Hanafy Morsy hafez abdelhafeez Department of Microbiology and Botany Faculty of Science Al-Zakazik University Al-Zakazik Egypt Department of Internal Medicine Al-Azhar School of Medicine Al-Azhar University Cairo Egypt Department of Clinical Pathology Al-Azhar School of Medicine Al-Azhar University Cairo Egypt

Background: Early promotion of hepatitis B (HB) vaccination among health care workers is an important component of the HBV infection control. No available data assess immune response of HB vaccination among Egyptian medical students. Objective: we conducted this study to evaluate the immune response among medical students after completion of their vaccination schedule. Methods: A total of 150 Egyptian medical students were included. Three doses of recombinant HB vaccine had been administered to all participating students at 0, 1 and 6 months. Antibody to hepatitis B surface antigen (Anti-HBs) titers, hepatitis B surface antigen (HBsAg), and total antibody to hepatitis B core antigen (anti-HBc) were measured by enzyme immunoassay, 1 to 2 months after completion of vaccination course. Results: Among 150 students included, the mean age was 22.4 ± 1.7 years (range 18 - 28 years). Fifty nine (39.4%) were males and 91 (60.6%) were females. All students have anti-HBs levels more than 100 IU/L. The mean anti-HBs of included students was 8994.2 ± 6373.1 IU/L. There was no significant difference of anti-HBs levels regarding age, sex, residence or body mass index distribution. Conclusion: Early HB vaccination of health care workers is associated with good immune response and should be encouraged.

关键词： HBV HB Vaccine Medical Students Anti-HBs Egypt

The Effectiveness of Intraoperative Frozen Section Analysis of Safety Margins in Breast Conserving Surgery and the Role of Surgeon in Decreasing the Rate of Positive Margins

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Surgical Science 2017年第12期8卷 499-509页

作者： Omar Farouk Ahmed Senbel Mosab Shetiwy Essam Attia Ahmed Abdallah Osama El-Damshety Ashraf Khater Sameh Roshdy Amr Abouzid Amr Hossam Islam H. Metwally Omar Hamdy Amir M. Zaid Mahmoud abdelaziz mohamed Elmetwally Adel Fathi Emad-Eldeen Hamed Khaled abdel Wahab Wagdi El-Kashef mohamed hafez Khaled Zalata mohamed Hegazy Adel Denewer Surgical Oncology Department Oncology Center Faculty of Medicine Mansoura University Mansoura Egypt Pathology Department Faculty of Medicine Mansoura University Mansoura Egypt

Introduction: Nowadays the more accepted surgical option for treating early breast cancer is breast conserving surgery. The main challenge in this type of surgery is to get free safety margins without need of second surgical operation, so many breast surgeons have started to depend on intraoperative frozen sections to ensure free safety margins. Aim of work: To assess our policy that we prefer to depend on intraoperative frozen section analysis to get free safety margin in breast conserving surgery from the 1st surgery, and its oncologic outcome. Patients and Methods: This is a retrospective study conducted in Oncology Center—Mansoura University (OCMU), where the data of 219 patients with breast cancer, who were managed by breast conserving surgery with intraoperative frozen section analysis of the safety margins, was analyzed. Results: The intraoperative frozen section analysis of safety margin was negative from the start in 183 (83.6%) patients, while it was positive in 36 patients (16.4%). Intraoperative decision of margin re-excision was applied for 29 patients (13.2%) in order to reach negative margin, modified radical mastectomy was offered for 4 patients (1.8%), while nipple sparing mastectomy with immediate breast reconstruction using latissimus dorsi flap was offered for 3 patients (1.4%). The postoperative paraffin results were typical with intraoperative frozen section analysis results in 216 patients (98.6%) and different results were obtained in only 3 patients (1.4%) who were managed by modified radical mastectomy in a second operation. Only 4 patients had local recurrence (1.8%) during the period of follow-up duration which was ranged from 1 to 86 months with mean ± SD (22.3 ± 14.1). Conclusion: The intraoperative frozen section analysis of safety margins in breast conserving surgery has very high-rate typical results with the paraffin section analysis and it is very helpful in decreasing the rate of second surgical operation in cases of infiltrated m

关键词： Breast Conserving Surgery Intraoperative Frozen Section Analysis Safety Margins Breast