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Postnatal ex vivo rat model for longitudinal bone growth investigations

Animal Models and Experimental Medicine 2019年第1期2卷 34-43页

作者： Adamu Abdul Abubakar Sahar Mohammed Ibrahim Ahmed Khalaf ali Kareem Obayes Handool Mohammad Shuaib Khan Mohamed Noordin Mustapha Tengku Azmi Ibrahim Ubedullah Kaka Loqman mohamad Yusof Department of Companion Animal Medicine and Surgery Universiti Putra Malaysia Department of Veterinary Surgery and Radiology Usmanu Danfodiyo University Department of Surgery and Theriogenology College of Veterinary Medicine University of Mosul Faculty of Veterinary and Animal Science Gomal University Department of Veterinary Pathology and Microbiology Universiti Putra Malaysia Department of Pre-Clinical Veterinary Sciences Universiti Putra Malaysia Laboratory of Sustainable Animal Production and Biodiversity Institute of Tropical Agriculture and Food SecurityUniversiti Putra Malaysia

Background: Chondrocytes in the growth plate(GP) undergo increases in volume during different cascades of cell differentiation during longitudinal bone growth. The volume increase is reported to be the most significant variable in understanding the mechanism of long bone ***: Forty-five postnatal Sprague-Dawley rat pups, 7-15 days old were divided into nine age groups(P7-P15). Five pups were allocated to each group. The rats were sacrificed and tibia and metatarsal bones were harvested. Bone lengths were measured after 0, 24, 48, and 72 hours of ex vivo incubation. Histology of bones was carried out, and GP lengths and chondrocyte densities were ***: There were significant differences in bone length among the age groups after 0 and 72 hours of incubation. Histological sectioning was possible in metatarsal bone from all age groups, and in tibia from 7-to 13-day-old rats. No significant differences in tibia and metatarsal GP lengths were seen among different age groups at 0 and 72 hours of incubation. Significant differences in chondrocyte densities along the epiphyseal GP of the bones between 0 and 72 hours of incubation were observed in most of the age ***: Ex vivo growth of tibia and metatarsal bones of rats aged 7-15 days old is possible, with percentage growth rates of 23.87 ± 0.80% and 40.38 ± 0.95%measured in tibia and metatarsal bone, respectively. Histological sectioning of bones was carried out without the need for decalcification in P7-P13 tibia and P7-P15 metatarsal bone. Increases in chondrocyte density along the GP influence overall bone elongation.

关键词： bone growth model chondrocytes endochondral ossification growth plate Sprague-Dawley rat

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Relocation of hypocenters from DOMERAPI and BMKG networks： a preliminary result from DOMERAPI project

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Earthquake Science 2017年第2期30卷 67-79页

作者： mohamad Ramdhan Sri Widiyantoro Andri Dian Nugraha Jean-Philippe Métaxian Asep Saepuloh Said Kristyawan Andry Syaly Sembiring Agus Budi Santoso Antoine Laurin Ahmad ali Fahmi Study Program of Earth Sciences Faculty of Earth Science andTechnology Institute of Technology Bandung Jalan Ganesa No.10 Bandung 40132 Indonesia Agency for Meteorology Climatology and Geophysics(BMKG) Jakarta Indonesia Global Geophysics Research Group Faculty of Mining andPetroleum Engineering Institute of Technology Bandung JalanGanesa No. 10 Bandung 40132 Indonesia Research Center for Disaster Mitigation Institute of TechnologyBandung Jalan Ganesa No. 10 Bandung 40132 Indonesia ISTerre IRD R219 CNRS Universite de Savoie Mont BlancLe Bourget-du-Lac France Faculty of Earth Sciences and Technology Institute of Technology Bandung Jalan Ganesa No. 10 Bandung 40132Indonesia Center for Volcanology and Geological Hazard Mitigation Geological Agency Jalan Diponegoro No. 57 Bandung 40122Indonesia

Merapi volcano located in central Java, Indonesia,is one of the most active stratovolcanoes in the world. Many Earth scientists have conducted studies on this volcano using various methods. The geological features around Merapi are very attractive to be investigated because they have been formed by a complex tectonic process and volcanic activities since tens of millions of years ago. The southern mountain range, Kendeng basin and Opak active fault located around the study area resulted from these processes. DOMERAPI project was conducted to understand deep magma sources of the Merapi volcano comprehensively. The DOMERAPI network was running from October 2013 to mid-April 2015 by deploying 46 broad-band seismometers around the volcano. Several steps, i.e., earthquake event identification,arrival time picking of P and S waves, hypocenter determination and hypocenter relocation, were carried out in this study. We used Geiger＇s method（Geiger 1912） for hypocenter determination and double-difference method for hypocenter relocation. The relocation result will be used to carry out seismic tomographic imaging of structures beneath the Merapi volcano and its surroundings. For the hypocenter determination, the DOMERAPI data were processed simultaneously with those from the Agency for Meteorology, Climatology and Geophysics（BMKG） seismic network in order to minimize the azimuthal gap. We found that the majority of earthquakes occurred outside the DOMERAPI network. There are 464 and 399 earthquakes obtained before and after hypocenter relocation, respectively. The hypocenter relocation result successfully detects some tectonic features, such as a nearly vertical cluster of events indicating a subduction-related backthrust to the south of central Java and a cluster of events to the east of Opak fault suggesting that the fault has an eastward dip.

关键词： Earthquake Relocation DOMERAPI BMKG Tectonic feature

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Diversity of Nocardia species isolated from transplantation and cancer centers of Tehran hospitals

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Journal of Acute Disease 2019年第6期8卷 255-259页

作者： ali Javadi mohamad Reza Pourmand Javad Hamedi Seyyed Saeed Eshraghi Department of Pathobiology School of Public HealthTehran University of Medical SciencesTehranIran Microbial Biotechnology Laboratory Department of MicrobiologySchool of BiologyCollege of ScienceUniversity of TehranTehranIran

Objective:To identify the biodiversity of Nocardia species isolated from the environments of Tehran hospitals via phenotypic and PCR molecular ***:A total of 236 samples were collected from the transplantation and cancer centers of five Tehran hospitals,and the presence of Nocardia was identified by using standard *** tests were used for preliminary identification,and PCR amplification of the 598 bp amplicon of the 16S rRNA and sequence analysis of 16S rRNA were performed for genus and species ***:From the 236 soil samples,16 samples were identified as positive based on the culture tests,while 8 samples showed positive results based on the molecular *** phylogenetic tree showed that the most frequent genus of Nocardia was related to Nocardia ***:The hospital environment is a potential reservoir of a diverse range of Nocardia *** to the strong capability of survival,these bacteria bring a threat to the health of *** identification can help physicians to implement treatments and is important for healthcare centers.

关键词： Nocardia Transplantation center Cancer center Soil samples Molecular identification

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Nuclear Fuel Cell Calculation Using Collision Probability Method with Linear Non Flat Flux Approach

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World Journal of Nuclear Science and Technology 2012年第2期2卷 49-53页

作者： mohamad ali Shafii Zaki Su’ud Abdul Waris Neny Kurniasih Nuclear and Biophysics Research Group Faculty of Mathematics and Natural Sciences Bandung Institute of Technology Bandung Indonesia

Nuclear fuel cell calculation is one of the most complicated steps of neutron transport problems in the reactor core. A few numerical methods use neutron flat flux (FF) approximation to solve this problem. In this approach, neutron flux spectrum is assumed constant in each region. The solution of neutron transport equation using collision probability (CP) method based on non flat flux (NFF) approximation by introducing linear spatial distribution function implemented to a simple cylindrical annular cell has been carried out. In this concept, neutron flux spectrum in each region is different each other because of an existing of the spatial function. Numerical calculation of the neutron flux in each region of the cell using NFF approach shows a fairly good agreement compared to those calculated using existing SRAC code and FF approach. Moreover, calculation of the neutron flux in each region of the nuclear fuel cell using NFF approach needs only 6 meshes which give equivalent result when it is calculated using 24 meshes in FF approach. This result indicates that NFF approach is more efficient to be used to calculate the neutron flux in the regions of the cell than FF approach.

关键词： Nuclear Fuel Cell Calculation Neutron Flux Linear NFF Approximation

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Calculation of dpa rate in graphite box of Tehran Research Reactor(TRR)

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Nuclear Science and Techniques 2019年第6期30卷 44-56页

作者： mohamad Amin Amirkhani Mohsen Asadi Asadabad Mostafa Hassanzadeh Seyed Mohammad Mirvakili ali Mohammadi Reactor and Nuclear Safety Research School Nuclear Science and Technology Research Institute Tehran Iran Department of Physics Arak University Arak Iran

Radiation damage is an important factor that must be considered while designing nuclear facilities and nuclear materials. In this study, radiation damage is investigated in graphite, which is used as a neutron reflector in the Tehran Research Reactor (TRR) core. Radiation damage is shown by displacement per atom (dpa) unit. A cross section of the material was created by using the SPECOMP code. The concentration of impurities present in the non-irradiated graphite was measured by using the ICP-AES method. In the present study the MCNPX code had identified the most sensitive location for radiation damage inside the reactor core. Subsequently, the radiation damage (spectral-averaged dpa values) in the aforementioned location was calculated by using the SPECTER, SRIM Monte Carlo codes, and Norgett, Robinson and Torrens (NRT) model. The results of “Ion Distribution and Quick Calculation of Damage”(QD) method groups had a minor difference with the results of the SPECTER code and NRT model. The maximum radiation damage rate calculated for the graphite present in the TRR core was 1.567 9 10^-8 dpa/s. Finally, hydrogen retention was calculated as a function of the irradiation time.

关键词： Radiation damage Graphite SPECTER SRIM MCNPX Tehran Research Reactor

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A Comparison between Supra-Scarpa’s Fascia Abdominoplasty and Traditional Abdominoplasty: Effect of Caesarian Section

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Modern Plastic Surgery 2021年第2期11卷 37-48页

作者： ali Alrida Rahal Sinan Alboudi Mhd Husam Alhilbawi mohamad Raghid Hasan Anwar Alhassanieh Division of Plastic and Reconstructive Surgery Department of Surgery Faculty of Medicine Damascus University Damascus Syria

Background: Abdominoplasty is still among the most popular surgeries in the world of cosmetic surgery, as many modifications have been implemented on this surgery to improve aesthetics and reduce the ratio of complication, especially seroma. Also, the duration to drain removal increases the risk of infection, and this can be avoided by preserving the Scarpa’s fascia. Methods: The study was performed from January 2018 to December 2020. The patients were divided into two groups: group A with patients who were performed in Supra-Scarpa’s fascia abdominoplasty, and group B who were performed in traditional abdominoplasty;then group A was divided into two subgroups (A1 with caesarean history and A2 with no caesarean history) that differ in caesarean history or not, while a comparison was done between the groups regarding early outcomes. Results: A total of 40 full abdominoplasties were preformed (group A, 19 patients;group B, 21 patients);then group A was divided into two subgroups (group A1, 13 patients;A2, 6 patients);we found that the time until drain removal and its drain output in group A is less with a statistical significance, and the seroma was less to happen in group A with no statistical significance. However, the operative time in group A is higher with statistical significance. In the subgroups A1 and A2, there was no statistically significant difference in the results. Conclusion: Preservation of the Scarpa’s fascia reduces the time until drain removal, total drain output and the occurrence of seroma and the caesarean scar doesn’t affect the efficacy of preserving the Scarpa’s fascia.

关键词： Abdominoplasty Scarpa’s Fascia Caesarian Section

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Antimicrobial effect of Malaysian vegetables against enteric bacteria

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Asian Pacific Journal of Tropical Biomedicine 2016年第3期6卷 211-215页

作者： Hassanain Al-Talib Norliana Dalila mohamad ali Mohamed Harreez Suhaimi Siti Shafika Nabila Rosli Nurul Huda Othman Nur Ain Sakinah Mansor Amira Kartini Sulaiman Shah Nurul Syuhada Ariffin Alyaa Al-Khateeb Laboratory Medical Science Cluster Faculty of Medicine Universiti Teknologi MARA (UiTM) Faculty of Medicine Universiti Teknologi MARA (UiTM)

Objective: To investigate the antibacterial activities of green vegetables(pennywort,mint, garlic, parsley and celery) against four common enteric bacteria [Salmonella enterica(ATCC 25957)(S. enterica), Shigella flexneri(ATCC 12022)(S. flexneri),Escherichia coli(ATCC 43889)(E. coli) and Enterobacter cloacae(ATCC 13047)(E. cloacae)] as an alternative medicine for controlling food borne diarrhea disease and the synergistic effect of green vegetables against those ***: Five common vegetables(pennywort, mint, garlic, parsley and celery) were purchased and extracted. The antimicrobial activities of these extracts were tested against four common enteric bacteria(S. enterica, S. flexneri, E. coli and E. cloacae). Ten different concentrations of the extracts(from 640 to 1.25 mg/m L) were prepared and used for the study. The minimal inhibitory concentration(MIC) was determined by the broth dilution method. The antimicrobial activities were assessed by using both well diffusion and disc diffusion ***: Garlic extract showed excellent inhibitory effects on all enteric bacteria. Other plants(parsley, celery, mint and pennywort) were not effective against enteric *** MIC of garlic against S. flexneri and E. cloacae was 40 mg/m L. The MIC of S. enterica and E. coli were 20 and 10 mg/m L, respectively. The performance of the well diffusion method was better than that of the disc diffusion method with clear and sharp inhibition zones of tested bacteria against plant ***: Garlic had excellent antimicrobial effects against enteric bacteria and was recommended to be given to patients with gastroenteritis. The other vegetables(pennywort, mint, parsley and celery) showed no inhibitory effects on enteric bacteria but still can be used for its richness in vitamins and fibers. The performance of the well diffusion method was better than that of the disc diffusion method in detecting the antibacterial effects of green vegetables.

关键词： Antibacterial activities Green vegetables Enteric bacteria Inhibitory effects

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Automatic segmentation of Colon Cancer Cells Based on Active Contour Method： A New Approach

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Journal of Life Sciences 2013年第2期7卷 105-109页

作者： Jamal Charara Alaa Hilal ali Al Houseini Walid Hassan mohamad Nassreddine Department of Physics and Electronics Faculty of Sciences I Lebanese University Beirut Lebanon

Automatic interpretation of the images of colon cell biopsies requires automatic segmentation of these cells in the image obtained. The active contour method for image segmentation is a well known method for automatic detection of the cell contour. However, the application of this method on colon cell images was not effective. In this paper, the authors have proposed a new technique to reduce the analysis time needed to detect cells in a given image. This technique is based on the active contour method but now using a progressive division of the dimensions of the image to achieve convergence. The model proposed succeeded in detecting cells whose boundaries are not necessarily defined by a gradient. The initial curve can be anywhere in the image, and interior contours can be automatically detected. The developed algorithm was successfully applied on textured multispectral images of three types of cells, including benign hyperplasia （BH）, intraepithelial neoplasia （IN）, and carcinoma （Ca） cells.

关键词： Active contours multispectral image texture segmentation.

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An Empirical Study on Microbial Load and Acidity in Raw Milk Produced in Malayer and Nahavand Cities, Iran 2012

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Health 2014年第16期6卷 2184-2189页

作者： Mohammad Rezaei ali Asghar Behzadi ali Akbar Malekirad Ahmad Fattahi mohamad Hadi Farahzadi Mohammad Sarmadi Hasan Mohammadi Aghamirlou Reza ali Fallahzadeh Department of Food Safety and Hygiene School of Public Health Tehran University of Medical Sciences Tehran Iran Department of Food Safety and Hygiene School of Health Yazd University of Medical Sciences Yazd Iran Department of Biology Payame Noor University Tehran Iran Department of Occupational Health Engineering School of Health Shahid Sadoughi University of Medical Sci-ences Yazd Iran Department of Neuroscience School of Advanced Technologies in Medicine Tehran University of Medical Sciences Tehran Iran Department of Environmental Health and Engineering School of Public Health Tehran University of Medical Sciences Tehran Iran Department of Environmental Health Engineering School of Health Shahid Sadoughi University of Medical Sciences Yazd Iran

Milk is very susceptible for growth and development of many microorganisms because it is rich in nutrients and has suitable condition. Thus, it is very vital to respect sanitary conditions at all stages of production and transport of raw milk. The objective of the present investigation was to determine the total bacterial, and acidity in raw milk from milk collection centers in Malayer and Nahavand cities. 52 milk samples were collected from 13 centers in summer 2012 and were analyzed according to standard methods. None of the raw milk samples under investigation, had superior or grade 1 quality. Only 7.7% of the samples were classified as raw milk with grade 2 quality and 92.3% were non-standard. The mean of microbial total count in base was 3.8 × 107 CFU/ml and average acidity in the samples was 0.163. The city of Tochqhaz and aliabade-Damagh has the lowest and the highest microbial total count. In general the result of this study showed unsatisfactory conditions of milk in the milk collection centers of the Malayer and Nahavand cities.

关键词： Food Safety Milk Collection Centers Acidity Microbial Total Counts

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Automated Classification of Segmented Cancerous Cells in Multispectral Images

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Journal of Life Sciences 2013年第4期7卷 358-362页

作者： Alaa Hilal Jamal Charara ali Al Houseini Walid Hassan mohamad Nassreddine Signal and Image Processing Lab Faculty of Sciences I Lebanese University Beirut Lebanon

Automatic reading procedures in colon cells biopsies allow a faster and precise reading of microscopic biopsies. These procedures implement automatic image segmentation in order to classify cell types as cancerous or noncancerous. The authors have developed a new approach aiming to detect colon cancer cells derived from the ＂Snake＂ method but using a progressive division of the dimensions of the image to achieve rapid segmentation. The aim of the present paper was to classify different cancerous cell types based on nine morphological parameters and on probabilistic neural network. Three types of cells were used to assess the efficiency of our classifications models, including BH （Benign Hyperplasia）, IN （Intraepithelial Neoplasia） that is a precursor state for cancer, and Ca （Carcinoma） that corresponds to abnormal tissue proliferation （cancer）. Results showed that among the nine parameters used to classify cells, only three morphologic parameters （area, Xor convex and solidity） were found to be effective in distinguishing the three types of cells. In addition, classification of unknown cells was possible using this method.

关键词： Multispectral image classification morphologic parameters probabilistic neural network.

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