检索结果-南通市图书馆

Uridylation and adenylation of RNAs

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2015年第11期58卷 1057-1066页

作者： SONG JianBo SONG Jun mo beixin CHEN XueMei Shenzhen Key Laboratory of Microbial Genetic Engineering College of Life Science Shenzhen University Department of Biochemistry and molecular Biology College of Science Jiangxi Agricultural University Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Optoelectronic Engineering Shenzhen University Department of Botany and Plant Sciences Institute of Integrative Genome Biology University of California Howard Hughes Medical Institute University of California

The posttranscriptional addition of nontemplated nucleotides to the 3′ ends of RNA molecules can have a significant impact on their stability and biological function. It has been recently discovered that nontemplated addition of uridine or adenosine to the 3′ ends of RNAs occurs in different organisms ranging from algae to humans, and on different kinds of RNAs, such as histone m RNAs, m RNA fragments, U6 sn RNA, mature small RNAs and their precursors etc. These modifications may lead to different outcomes, such as increasing RNA decay, promoting or inhibiting RNA processing, or changing RNA activity. Growing pieces of evidence have revealed that such modifications can be RNA sequence-specific and subjected to temporal or spatial regulation in development. RNA tailing and its outcomes have been associated with human diseases such as cancer. Here, we review recent developments in RNA uridylation and adenylation and discuss the future prospects in this research area.

关键词： Uridylation adenylation miRNA pre-miRNA U6 snRNA histone mRNA rRNA

miR319-TCPs-TGA9/TGA10/ROXY2 regulatory module controls cell fate specification in early anther development in Arabidopsis

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2024年第4期67卷 839-842页

作者： Jinyuan Tao Zijie Pan Wenwen Kong beixin mo Xuemei Chen Yu Yu Guangdong Provincial Key Laboratory for Plant Epigenetics Longhua Bioindustry and Innovation Research InstituteCollege of Life Sciences and OceanographyShenzhen UniversityShenzhen 518060China Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Optoelectronic EngineeringShenzhen UniversityShenzhen 518060China School of Life Sciences Peking-Tsinghua Joint Center for Life SciencesPeking UniversityBeijing 100871China

Dear Editor,The process of anther development is generally conserved across plant species(Ma,2005).Many protein-coding genes and noncoding RNAs have been identified to participate in early anther development in Arabidopsis(Walbot and Egger,2016).mi R319 is one of the most ancient and conserved mi RNA families in land plants(Chávez montes et al.,2014).

关键词： Arabidopsis miR319 ancient

Increasing the efficiency of CRISPR/Cas9-based gene editing by suppressing RNAi in plants

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2019年第7期62卷 982-984页

作者： Xiaoyan Wang Jiayun Lu Kangwen Lao Suikang Wang Xiaowei mo Xintong Xu Xuemei Chen beixin mo Guangdong Provincial Key Laboratory for Plant Epigenetics College of Life Sciences and OceanographyShenzhen UniversityShenzhen 518060China Department of Botany and Plant Sciences Institute of Integrative Genome BiologyUniversity of CaliforniaRiversideCA 92521USA

Dear Editor,CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR-associated)-based genome editing is a powerful and widely adopted technology for introducing specific mutations in animal and... 详细信息

关键词： Editor CRISPR systems

Protein arginine methyltransferase 3 fine-tunes the assembly/disassembly of pre-ribosomes to repress nucleolar stress by interacting with RPS2B in arabidopsis

在线全文

学校读者我要写书评

暂无评论

molecular Plant 2021年第2期14卷 223-236页

作者： Runlai Hang Zhen Wang Chao Yang Lilan Luo beixin mo Xuemei Chen Jing Sun Chunyan Liu Xiaofeng Cao State Key Laboratory of Plant Genomics and National Center for Plant Gene Research Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China Guangdong Provincial Key Laboratory for Plant Epigenetics College of Life Sciences and OceanographyShenzhen UniversityShenzhen 518060China Department of Botany and Plant Sciences Institute of Integrative Genome BiologyUniversity of CaliforniaRiversideRiversideCA 92521USA College of Life Sciences University of Chinese Academy of SciencesBeijing 100039China CAS Center for Excellence in molecular Plant Sciences Chinese Academy of SciencesBeijing 100101China

Ribosome biogenesis,which takes place mainly in the nucleolus,involves coordinated expression of preribosomal RNAs(pre-rRNAs)and ribosomal proteins,pre-rRNA processing,and subunit assembly with the aid of numerous assembly *** previous study showed that the Arabidopsis thaliana protein arginine methyltransferase AtPRMT3 regulates pre-rRNA processing;however,the underlying molecular mechanism remains ***,we report that AtPRMT3 interacts with Ribosomal Protein S2(RPS2),facilitating processing of the 90S/Small Subunit(SSU)processome and repressing nucleolar *** isolated an intragenic suppressor of atprmt3-2,which rescues the developmental defects of atprmt3-2 while produces a putative truncated AtPRMT3 protein bearing the entire N-terminus but lacking an intact enzymatic activity domain We further identified RPS2 as an interacting partner of AtPRMT3,and found that loss-of-function rps2a2b mutants were phenotypically reminiscent of atprmt3,showing pleiotropic developmental defects and aberrant pre-rRNA ***2B binds directly to pre-rRNAs in the nucleus,and such binding is enhanced in ***,multiple components of the 90S/SSU processome were more enriched by RPS2B in atprmt3-2,which accounts for early pre-rRNA processing defects and results in nucleolar ***,our study uncovered a novel mechanism by which AtPRMT3 cooperates with RPS2B to facilitate the dynamic assembly/disassembly of the 90S/SSU processome during ribosome biogenesis and repress nucleolar stress.

关键词： AtPRMT3 RPS2 ribosome assembly pre-rRNA processing 90S/SSU processome nucleolar stress

A simple and efficient carbon nanotube-based nanocarriers simultaneously delivers multiple plasmids into diverse mature tissues of monocotyledonous crops

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2023年第7期66卷 1701-1704页

作者： Xuedong Liu Wenwen Kong Nian Xiao Lijuan Huang Yu Zhang Weizhao Chen beixin mo Yu Yu Guangdong Provincial Key Laboratory for Plant Epigenetics Longhua Bioindustry and Innovation Research InstituteCollege of Life Sciences and OceanographyShenzhen UniversityShenzhen518060China Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Optoelectronic EngineeringShenzhen UniversityShenzhen518060China Instrumental Analysis Center of Shenzhen University(Lihu Campus) Shenzhen UniversityShenzhen518060China

Dear Editor,With the development of high-throughput sequencing technology,a large number of functional genes have been ***,the illustration of these gene functions is limited,especially in monocot plants(Chen et al.,2022).One of the main reasons is that genetic transformation is still a major bottleneck for these ***,nanoparticles(NPs)-mediated biomolecular delivery methods.

关键词： mono illustration crops

Study on RNAi-based herbicide for Mikania micrantha

在线全文

学校读者我要写书评

暂无评论

Synthetic and Systems Biotechnology 2021年第4期6卷 437-445页

作者： Jiantao Mai Lingling Liao Rongsong Ling Xiaolong Guo Jingying Lin beixin mo Weizhao Chen Yu Yu Guangdong Provincial Key Laboratory for Plant Epigenetics Longhua Bioindustry and Innovation Research InstituteCollege of Life Sciences and OceanographyShenzhen University1066 Xueyuan AvenueShenzhen518000PR China Institute for Advanced Study Shenzhen University3688 Nanhai AvenueShenzhen518000PR China College of Materials Science and Engineering Shenzhen University1066 Xueyuan AvenueShenzhen518000PR China Shenzhen Key Laboratory for Microbial Gene Engineering College of Life Sciences and OceanographyShenzhen UniversityShenzhen518060PR China

The invasive plant Mikania micrantha Kunth(***)from South America poses a significant threat to the stability and biodiversity of ***,an effective and economical method to control *** is still *** interference(RNAi)has been widely studied and applied in agriculture for trait ***-induced gene silencing(SIGS)can produce RNAi silencing effects without introducing heritable modifications to the plant genome and is becoming a novel nontransformation strategy for plant *** this study,the genes encoding chlorophyll a/b-binding proteins were selected as targets of RNAi,based on high-throughput sequencing of *** transcriptome and bioinformatic analyses of sequence *** types of RNAi molecules,double-stranded RNA,RNAi nanomicrosphere,and short hairpin RNA(shRNA),with their corresponding short interfering RNA sequences were designed and synthesized for SIGS vector construction,from which each RNAi molecule was transcribed and extracted to be sprayed on *** *** water-treated control leaves remained green,leaves treated with RNAi molecules turned yellow and eventually *** real-time PCR showed that the expression levels of target genes were significantly reduced in the RNAi-treated groups compared with those of the control,suggesting that all three types of RNAi herbicides effectively silenced the endogenous target genes,which are essential for the growth of *** also found that shRNA showed better silencing efficiency than the other two *** together,our study successfully designed three types of RNAi-based herbicides that specifically silenced endogenous target genes and controlled the growth of ***,we identified a gene family encoding chlorophyll a/b-binding proteins that is important for the growth and development of *** and could serve as potential targets for controlling the spread of ***.

关键词： Invasive plant Mikania micrantha RNA interference Spray-induced gene silencing Chlorophyll a/b-binding protein Nucleic acid bioherbicide

NTP4 modulates miRNA accumulation via asymmetric modification of miRNA/miRNA duplex

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2021年第5期64卷 832-835页

作者： Wenwen Kong Xianxin Dong Yongbing Ren Yuan Wang Xintong Xu beixin mo Yu Yu Xiaoyan Wang Guangdong Provincial Key Laboratory for Plant Epigenetics Longhua Institute of Innovative BiotechnologyCollege of Life Sciences and OceanographyShenzhen UniversityShenzhen 518060China Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Optoelectronic EngineeringShenzhen UniversityShenzhen 518060China Advanced Institute of Natural Sciences Beijing Normal University at ZhuhaiZhuhai 519087China State Key Laboratory of Genetic Engineering School of Life SciencesFudan UniversityShanghai 200438China

Dear ***(miRNAs)are endogenous small RNAs(sRNAs)that play crucial regulatory roles in gene *** plants,the transcribed primary miRNAs(pri-miRNAs)are sequentially processed by DICER-LIKE 1(DCL1)into precursor miRNAs(pre-miRNAs),and eventually miRNA/miRNA*duplexes(Wang et al.,2019).The methyltransferase HUA ENHANCER 1(HEN1)confers 2’-Omethylation on the 3’terminal ribose of each strand within the miRNA/miRNA*duplexes(Yu et al.,2005).Once methylated,miRNA duplexes are loaded onto ARGONAUTE1(AGO1),with one strand referred to as miRNA which is stabilized to form RNA-induced silencing complexes(RISCs),whereas the other strand becomes miRNA*that is eliminated and degraded.

关键词： expression. al. duplex

NAD^(+)-capped RNAs are widespread in rice(Oryza sativa)and spatiotemporally modulated during development

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2022年第10期65卷 2121-2124页

作者： Haijiao Dong Xufeng Wang Cong Tan Lei Gao Jie Cui Lin Liu beixin mo Yongzhong Xing Yu Yu Xuemei Chen Guangdong Provincial Key Laboratory for Plant Epigenetics Longhua Bioindustry and Innovation Research InstituteCollege of Life Sciences and OceanographyShenzhen UniversityShenzhen 518060China Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Optoelectronic EngineeringShenzhen UniversityShenzhen518060China Department of Botany and Plant Sciences Institute of Integrative Genome BiologyUniversity of CaliforniaRiversideCA92521USA China National Gene Bank BGI-ShenzhenShenzhen 518120China National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(Wuhan) Huazhong Agricultural UniversityWuhan430070China

Dear Editor,Nicotinamide adenine dinucleotide(NAD+),a nucleotidecontaining metabolite,can be incorporated as an RNA 5′cap to form NAD^(+)-capped RNAs(NAD-RNAs),which have been identified in bacteria,yeast,mammalian cells and Arabidopsis thaliana(Hu et al.,2021).most NAD-RNAs are m RNAs encoded by the nuclear and mitochondrial genomes。

关键词： sativa widespread

同方期刊数据库

Targeted Genome Engineering and Its Application in Trait Improvement of Crop Plants

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences 2019年第10期10卷 1312-1342页

作者： Xiaoyu Yang Linlin Luo beixin mo Lin Liu Guangdong Provincial Key Laboratory for Plant Epigenetics Longhua Bioindustry and Innovation Research Institute College of Life Sciences and Oceanography Shenzhen University Shenzhen China Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Optoelectronic Engineering Shenzhen University Shenzhen China.

Targeted genome engineering refers to technologies that are used for site-specific genome modifications such as knockout, knockin and transcriptional regulation of genes of interest in organisms. Site-specific recombination system, zinc finger nucleases (ZFNs), transcriptional activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein-9 nuclease (Cas9) (CRISPR/Cas9) technologies are the representatives of targeted genome engineering and have been widely used in crop basic and applied research. In this review, we introduce the basic information and action modes of these different genome engineering technologies, summarize the recent progresses of targeted genome engineering technologies and their applications in crop improvement, and propose perspectives for genome engineering-mediated modifications of crop plants in the future.

关键词： Targeted Genome Engineering Site-Specific Recombination ZFN TALEN CRISPR/Cas9 Crop Improvement