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Journal of Life Sciences 2018年第3期12卷 134-140页

作者： Vanessa Bagnarello-Madrigal Daniela Rodríguez-Chaves Javier Alpizar-Cordero Alonso Calvo-Vargas Maribel Cordero-Villalobos misael chinchilla-carmona Idalia Valerio-Campos Ronald Sánchez -Porras Departamento de Química Universidad de Ciencias Médicas (UCIMED) San José 10108 Costa Rica Departamento de Investigación Básica Universidad de Ciencias Médicas (UCIMED) San José 10108 Costa Rica Sección de Biología Sede Occidente Universidad de Costa Rica San José 10108 Costa Rica

Despite the breadth of knowledge about malaria, its transmission, preventive measures and available treatments and its difficulty in controlling the vector and the rapid spread of resistance to current drugs, there is a stimulus to develop new research looking for active molecules. The phytochemical studies of the active extract confirmed the presence of alkaloids, flavonoids, coumarins, tannins, reducing compounds, anthocyanins, terpenes, triterpenes and steroids. In the present work, it was possible to isolate a mixture of isoquinolinic alkaloids from the tender leaves of Nectandra membranacea （Laureaceae） with antimalarial activity. The mixture composed of （-） - gliazovine and （＋） - aplogliazovine showed an IC50 value of 32.18 μg/mL against the P. berghei NK65 strain. It is important to continue with these studies and their chemical components isolated from N. membranacea that could be used as an alternative the treatment of malaria.

关键词： Malaria Nectandra membranacea alkaloids phytochemical screening Laureaceae.

Implementation of a New Solution for the Preservation of Anatomical Specimens Made of Non-Toxic Substances

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Open Journal of Veterinary Medicine 2024年第3期14卷 56-67页

作者： Ma Reyes Pichardo-Molinero Samantha Jardon-Xicotencatl misael R. Oliver-González Carlos G. García-Tovar Laboratorio de Apoyo Técnico a la Anatomía Facultad de Estudios Superiores Cuautitlán Universidad Nacional Autónoma de México Estado de México Mexico Unidad de Investigación Multidisciplinaria L4 (Morfología Veterinaria y Biología Celular) Facultad de Estudios Superiores Cuautitlán Universidad Nacional Autónoma de México Estado de México Mexico

The preservation of anatomical pieces in Veterinary Anatomy is essential since it is not possible to dissect all domestic species. Most techniques use reagents with high levels of toxicity such as formaldehyde. The objective of this work was to develop a new preservation technique that uses reagents with zero toxicity and that allows obtaining preserved pieces suitable for anatomical studies. The alcohol propylene glycol technique was developed, the method of which uses a fixation step with alcohol, sodium chloride, commercial vinegar and subsequently the impregnation of the preservation solution made from propylene glycol and commercial vinegar, which are non-toxic. As a result of this work, adequately preserved sheep hearts were obtained that preserved their morphology with slight changes in size and weight, which did not affect their external and internal anatomical structure. Its coloration was not substantially affected, remaining a little lighter. The pieces obtained showed flexibility which allowed dissections to be carried out. The time to develop the technique was 20 days. A comparative study was carried out with the phenolated glycerin technique that uses toxic reagents (formaldehyde and phenol) and the pieces obtained with the alcohol propylene glycol technique were of better quality, observing that the pieces with phenolated glycerin tend to darken and are more rigid. And the time to develop the technique is 24 days. In conclusion, a preservation technique for anatomical pieces was developed that allowed the preservation of the organs under study, which allow their use for anatomical studies, and which have been preserved without changes until the time of this publication (8 months) and there are pieces preserved with this technique for 2 years.

关键词： Veterinary Anatomy Heart Preservation Techniques Alcohol Propylene Glycol Technique Fixation Necropsy Toxic Chemicals

Clinical heterogeneity in autoimmune acute liver failure

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World Journal of Gastroenterology 2007年第12期13卷 1824-1827页

作者： Norberto C Chavez-Tapia Julio Martinez-Salgado Julio Granados misael Uribe Felix I Tellez-Avila Department of Gastroenterology Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán Mexico City MexicoJulio Granados Department of Immunology and Rheumatology Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán Mexico City Mexico Department of Immunology and Rheumatology Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán Mexico City Mexico

AIM: To describe the outcome and prognosis in a cohort of patients with acute liver failure due to autoimmune hepatitis without liver transplantation. METHODS: A retrospective trial was conducted in 11 patients with acute liver failure due to autoimmune hepatitis who attended the Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubiran. Demographic, biochemical and severity indexes, and treatment and outcome were assessed. RESULTS: Among the 11 patients, with a median age of 31 years, 72% had inflammatory response syndrome, and six patients received corticosteroids. The mortality rate within four weeks was 56%, and the one-year survival was 27%. In the survivors, severity indexes were lower and 83% received corticosteroids. CONCLUSION: We observed a relatively high survival rate in patients with acute liver failure due to autoimmune hepatitis. This survival rate could be influenced by severity of the disease and/or use of corticosteroids.

关键词： Autoimmune hepatitis Acute liver failure Corticosteroids

Smoking is not associated with nonalcoholic fatty liver disease

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World Journal of Gastroenterology 2006年第32期12卷 5196-5200页

作者： Norberto C Chavez-Tapia Javier Lizardi-Cervera Oliver Perez-Bautista Martha H Ramos-Ostos misael Uribe Departments of Gastroenterology and Internal Medicine. Medica Sur Clinic and Foundation Mexico City Mexico

AIM： To analyze the relationship between smoking and nonalcoholic fatty liver disease （NAFLD）. METHODS： This is a cross-sectional study of a healthy population, carried out in a check-up unit of a university hospital in Mexico City. We enrolled 933 subjects, 368 current smokers （cases） and 565 persons who had never smoked （controls）. Demographic, metabolic and biochemical variables were measured in the two groups. NAFLD was determined by ultrasound and metabolic syndrome according to ATPⅢ. RESULTS： A total of 548 men （205 cases and 343 controls） and 337 women （114 cases and 223 controls） were included in the analysis. Statistical differences between cases and controls were observed only in high blood pressure prevalence （6.6% vs 11.3%, P〈0.05; cases and controls respectively）, high-density lipoproteins （1.00±0.26 vs 1.06±0.28 mmol/L, P 〈 0.005）, triglycerides （2.18±1.49 vs 1.84±1.1 mmol/L, P〈0.001）, and erythrocyte sedimentation rate （11.3±9.3 vs 13.5±11.9 mm/h, P〈0.001）. No differences were observed in the prevalence of NAFLD （22.27% vs 29.68%, P = NS） and metabolic syndrome （41.69% vs 36.74%, P = NS）. Univariate analysis showed that smoking was not a risk factor for NAFLD （OR = 0.89, 95% CI 0.65-1.21）. CONCLUSION： No differences in NAFLD prevalence were observed between current smokers and nonsmokers, and furthermore, no differences were observed in heavy smokers （more than 20 packs/year）, indicating that there js no relationship between smoking and NAFLD.

关键词： Smoking Nonalcoholic fatty liver disease Metabolic syndrome

Factors that influence outcome in non-invasive and invasive treatment in polycystic liver disease patients

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World Journal of Gastroenterology 2008年第20期14卷 3195-3200页

作者： Josué Barahona-Garrido Jesús Camacho-Escobedo Eduardo Cerda-Contreras Jorge Hernández-Calleros Jesús K Yamamoto-Furusho Aldo Torre misael Uribe Department of Gastroenterology National Institute of Health Sciences and Nutrition "Salvador Zubirán" Mexico City 14000 Mexico

AIM: To evaluate the factors that influence outcome of both non-invasive and invasive treatment of polycystic liver disease. METHODS: Analysis of clinical files of patients with complete follow-up from July 1986 to June 2006. RESULTS: Forty-one patients (male, 7; female, 34), 47.8 ± 11.9 years age, and 5.7 ± 6.7 years follow-up, were studied. Alkaline phosphatase (AP) elevation (15% of patients) was associated with the requirement of invasive treatment (IT, P = 0.005). IT rate was higher in symptomatic than non-symptomatic patients (65.4% vs 14.3%, P = 0.002), and in women taking hormonal replacement therapy (HRT) (P = 0.001). Cysts complications (CC) were more frequent (22%) in the symptomatic patients group (P = 0.023). Patients with body mass index (BMI) > 25 (59%) had a trend to complications after IT (P = 0.075). Abdominal pain was the most common symptom (56%) and indication for IT (78%). Nineteen patients (46%) required a first IT: 12 open fenestration (OF), 4 laparoscopic fenestration (LF) and 3 fenestration with hepatic resection (FHR). Three required a second IT, and one required a third procedure. Complications due to first IT were found in 32% (OF 16.7%, LF 25%, FHR 66.7%), and in thesecond IT in 66.7% (OF 100%). Follow-up mortality rate was 0. CONCLUSION: Presence of symptoms, elevated AP, and CC are associated with IT requirement. HRT is associated with presence of symptoms and IT requirement. Patients with BMI > 25 have a trend be susceptible to IT complications. The proportions of complications are higher in FHR and second IT groups. RS is more frequent after OF.

关键词： Hepatic cysts Open fenestration Laparoscopic fenestration Hepatic resection Recurrenceof symptoms Hormonal replacement therapy

Insulin sensitizers in treatment of nonalcoholic fatty liver disease:Systematic review

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World Journal of Gastroenterology 2006年第48期12卷 7826-7831页

作者： Norberto C Chavez-Tapia Tonatiuh Barrientos-Gutierrez Felix I Tellez-ávila Francisco Sánchez-ávila Maria Antonieta Montao-Reyes misael Uribe Department of Gastroenterology Instituto Nacional de Ciencias Médicas y Nutrición Zalvador Zubirán Mexico City Mexico The University of Texas School of Public Health Houston United States Instituto de Educación Media Superior Mexico City Mexico

AIM: To summarize the evidence available for the clinical effectiveness of insulin sensitizers in the treatment of nonalcoholic fatty liver disease (NAFLD) systematically. METHODS: Relevant articles were located using computer-assisted searches of Medline (1966-March 2006), EMBASE (1988-March 2006), CINAHL (1982-March 2003), Educational Resource Information Center (1966-March 2006), Library, Information Science & Technology Abstracts (1967-March 2006), Cochrane Database of Systematic Reviews, Database of Abstracts of Reviews of Effects (1994-2006), dissertations in ProQuest and FirstSearch databases. Manual searches were made in the abstracts from meetings of the American Gastroenterological Association (1999-2006), and the American Association for the Study of Liver Diseases (2003-2005). Studies were retrieved using the following selection criteria: (1) clinical trials using insulin sensitizers in subjects with NAFLD, (2) adult patients, (3) published as full manuscripts or abstracts, and (4) English, Spanish, German, and French languages only. Data were abstracted independently by two reviewers following standardized procedures. A face-to- face comparison of data was conducted to ensure the completeness and reliability of the abstraction process. RESULTS: Nine studies were included, six using metformin and three using thiazolidinediones. Only two studies were placebo-controlled trials. The mediansample size for all studies was 18 subjects. In the placebo-controlled trials, metformin improved insulin resistance markers and liver function tests, but not histological scores. In the single-arm trials, metformin and thiazolidinediones improved insulin resistance markers and liver function tests, and beneficial histological changes were reported. There is limited high-quality information available from which to draw categorical conclusions about the clinical use of insulin sensitizers in ***: Current information indicates that the use of insulin sensitizers

关键词： Steatohepatitis Evidence based medicine Systematic review

Metabolic syndrome as a risk factor for gallstone disease

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World Journal of Gastroenterology 2005年第11期11卷 1653-1657页

作者： Nahum Méndez-Sánchez Norberto C. Chavez-Tapia Daniel Motola-Kuba Karla Sanchez-Lara Guadalupe Ponciano-Rodríguez Héctor Baptista Martha H. Ramos misael Uribe Departments of Biomedical Research and Gastroenterology and Liver Unit

AIM: To establish an association between the presence of metabolic syndrome and the development of gallstone disease. METHODS: We carried out a cross-sectional study in a check-up unit in a university hospital in Mexico City. We enrolled 245 subjects, comprising 65 subjects with gallstones (36 women, 29 men) and 180 controls (79 women and 101 men without gallstones). Body mass index, waist circumference, blood pressure, plasma insulin, and serum lipids and lipoproteins levels were measured. Insulin resistance was calculated by homeostasis model assessment. Unconditional logistic regression analysis (univariate and multivariate) was used to calculate the risk of gallstone disease associated with the presence of at least three of the criteria (Adult Treatment Panel Ⅲ). Analyses were adjusted for age and sex. RESULTS: Among 245 subjects, metabolic syndrome was present in 40% of gallstone disease subjects, compared with 17.2% of the controls, adjusted by age and gender (odds ratio (OR) = 2.79; 95%CI, 1.46-5.33; P= 0.002), a dose-dependent effect was observed with each component of metabolic syndrome (OR=2.36, 95%CI, 0.72-7.71; P= 0.16 with one component and OR = 5.54, 95%CI, 1.35-22.74; P = 0.02 with four components of metabolic syndrome). Homeostasis model assessment was significantly associated with gallstone disease (adjusted OR = 2.25; 95%CI, 1.08-4.69; P= 0.03). CONCLUSION: We conclude that as for cardiovascular disease and diabetes mellitus, gallstone disease appears to be strongly associated with metabolic syndrome.

关键词： Obesity Metabolic syndrome Gallstones Insulin resistance

Endoscopic submucosal dissection in dogs in a World Gastroenterology Organisation training center

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World Journal of Gastroenterology 2010年第14期16卷 1759-1764页

作者： Miguel A Tanimoto Gonzalo Torres-Villalobos Rikiya Fujita Patricio Santillan-Doherty Jorge Albores-Saavedra German Gutierrez Luis A Martin-del-Campo Carlos Bravo-Reyna Octavio Villanueva Jose J Villalobos misael Uribe Miguel A Valdovinos Department of Gastroenterology and World Gastroenterology Organisation Training Center National Institute of Medical Sciences and Nutrition Salvador Zubiran Mexico City 14000 Mexico Department of Surgery and Experimental Surgery National Institute of Medical Sciences and Nutrition Salvador Zubiran Mexico City 14000 Mexico The Cancer Institute Hospital of JFCR Tokyo Tokyo 135-8550 Japan Department of Pathology National Institute of Medical Sciences and Nutrition Salvador Zubiran Mexico City 14000 Mexico Department of Experimental Surgery National Institute of Medical Sciences and Nutrition Salvador Zu- biran Mexico City 14000 Mexico Animal lab and Department of Experimental Research National Institute of Medical Sciences and Nutrition Salvador Zubiran Mexico City 14000 Mexico

AIM: To evaluate if canine models are appropriate for teaching endoscopy fellows the techniques of endoscopic submucosal dissection (ESD). METHODS: ESD was performed in 10 canine models under general anesthesia, on artificial lesions of the esophagus or stomach marked with coagulation points. After ESD, each canine model was euthanized and surgical resection of the esophagus or stomach was carried out according to "The Principles of Humane Experimental Technique, Russel and Burch". The ESD specimens were fixed with needles on cork submerged in a formol solution with the esophagus or stomach, and delivered to the pathology department to be analyzed. RESULTS: ESD was completed without complications using the Hook-knife in five esophageal areas, with a procedural duration of 124 ± 19 min, a length of 27.4 ± 2.6 mm and a width of 21 ± 2.4 mm. ESD was also completed without complications using the IT-knife2 in five gastric areas, with a procedural duration of 92.6 ± 19 min, a length of 32 ± 2.5 mm and a width of 18 ± 3.7 mm. CONCLUSION: ESD is feasible in the normal esopha- gus and stomach of canine models, which are appropriate for teaching this technique.

关键词： Endoscopic mucosal resection Endoscopic submucosal dissection Stomach neoplasms Training

Adiponectin as a protective factor in hepatic steatosis

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World Journal of Gastroenterology 2005年第12期11卷 1737-1741页

作者： Nahum Méndez-Sánchez Norberto C.Chávez-Tapia Antonio R.Villa Karla Sánchez-Lara Daniel Zamora-Valdés Martha H.Ramos misael Uribe Liver Research Unit Clinical Epidemiology Unit

AIM: Obesity and insulin resistance (IR) are closely related to hepatic steatosis (HS), and adiponectin is a hepatic insulin sensitizer that has important effects in liver function. This study aims at investigating the relationship between serum adiponectin concentration and the presence of HS. METHODS: We carried out a cross-sectional study in a check-up unit of a University Hospital in Mexico City. We enrolled 196 subjects, comprising 98 subjects with HS (27 women, 71 men) and 98 controls (37 women and 61 men). Anthropometric, metabolic and biochemical variables were measured in the two groups. Serum adiponectin and leptin concentrations were determined, their association with grade of HS tested, and concentrations, according to quartiles, compared between cases and controls. X2 analysis for linear trends was used to test for a dose-response relationship and logistic regression analysis was conducted to test for a protective effect of adiponectin. RESULTS: The HS subjects were older and more obese than controls, with a central obesity pattern. In the fourth quartile of adiponectin concentrations, HS was less common and severe. In a multivariate model of the fourth quartile of the adiponectin concentrations, we observed a protective effect (OR = 0.17, 95%CI: 0.04-0.67, P= 0.01). In subjects with more severe HS, we observed higher leptin concentrations, and caloric intakes, total fat and iron consumption were higher than in controls. CONCLUSION: The results of the present study suggest that a high serum concentration of adiponectin is associated with a protective effect against HS.

关键词： Adiponectin Hepatic steatosis Leptin Diet

Geochemistry and Heavy Metal Levels in the Sediments of the Port of Santa Bárbara de Samana, Dominican Republic

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Journal of Geoscience and Environment Protection 2021年第4期9卷 195-205页

作者： Ramón Delanoy misael Díaz Asencio Darlin Rodriguez Ministry of Higher Education Science and Technology Institute of Physics Autonomous University of Santo Domingo Santo Domingo Dominican Republic Division of Oceanology Ensenada Center for Scientific Research and Higher Education Ensenada México Department of Physics Autonomous University of Santo Domingo Santo Domingo Dominican Republic

In the Port of Santa Bárbara de Samana, chemical residues, organic matter and heavy metals from domestic activities are deposited together in the waters and sediments. The analysis of the sediments by X-ray fluorescence of four extracted and sectioned cores showed that concentrations of trace metals such as Nickel, Chromium, Lead and Mercury were present at various depths, exceeding Limits of Toxicity (PEL) for marine sediments according to National Oceanic and Atmospheric Administration (NOAA) and Canadian Council of Ministers of the Environment (CCME). Cadmium presented values above the toxicity threshold (TEL) in its minimum values and in its maximum values they exceeded the PEL value. While the Zinc and Copper values were low in all sections and lower than TEL. The analysis of the loss by ignition and the dating with lead 210 due to excess of the C4 core, showed a sudden change in the organic matter content and sedimentation rate. The superficial sediments show that unlike the deeper ones, the heavy metal content is lower, as well as that they do not represent a risk to the ecosystem by not exceeding toxicity levels.

关键词： Sediment Heavy Metal Port of Samana X-Ray Fluorescence Mercury