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Corrigendum to“The effective transfection of a low dose of negatively charged drug-loaded DNA-nanocarriers into cancer cells via scavenger receptors”[Journal of Pharmaceutical Analysis volume 11(2021)174e182]

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Journal of Pharmaceutical Analysis 2022年第4期12卷 698-700页

作者： mirza muhammad faran ashraf baig Chengfei Zhang muhammad Furqan Akhtar Ammara Saleem Jahanzeb Mudassir Laboratory of Biomedical&Pharmaceutical Engineering of Stem Cells Research Restorative Dental SciencesFaculty of DentistryThe University of Hong KongHong Kong999077PR China State Key Laboratory of Analytical Chemistry for Life Sciences School of Chemistry and Chemical EngineeringNanjing UniversityNanjing210023PR China Faculty of Pharmacy Bahauddin Zakariya UniversityMultan 60000Pakistan Riphah Institute of Pharmaceutical Sciences Riphah International UniversityLahorePakistan Department of Pharmacology Faculty of Pharmaceutical SciencesGovernment College University FaisalabadFaisalabadPakistan

The authors regret to inform that Figs.4,6,and 7 in the original article were wrongly *** corrected figures appear below:The authors would like to apologize for any inconvenience caused.

关键词： Cor loaded cancer

The effective transfection of a low dose of negatively charged drug-loaded DNA-nanocarriers into cancer cells via scavenger receptors

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Journal of Pharmaceutical Analysis 2021年第2期11卷 174-182页

作者： mirza muhammad faran ashraf baig Chengfei Zhang muhammad Furqan Akhtar Ammara Saleem Jahanzeb Mudassir Laboratory of Biomedical&Pharmaceutical Engineering of Stem Cells Research Restorative Dental SciencesFaculty of DentistryThe University of Hong Kong999077Hong KongPR China State Key Laboratory of Analytical Chemistry for Life Sciences School of Chemistry and Chemical EngineeringNanjing UniversityNanjing210023PR China Faculty of Pharmacy Bahauddin Zakariya UniversityMultan60000Pakistan Riphah Institute of Pharmaceutical Sciences Riphah International UniversityLahorePakistan Department of Pharmacology Faculty of Pharmaceutical SciencesGovernment College University FaisalabadFaisalabadPakistan

DNA-nanotechnology-based nano-architecture scaffolds based on circular strands were designed in the form of DNA-nanowires(DNA-NWs) as a polymer of DNA-triangles. Circularizing a scaffold strand(84-NT) was the critical step followed by annealing with various staple strands to make stiff DNAtriangles. Atomic force microcopy(AFM), native polyacrylamide gel electrophoresis(PAGE), UVanalysis, MTT-assay, flow cytometry, and confocal imaging were performed to assess the formulated DNA-NWs and cisplatin(CPT) loading. The AFM and confocal microscopy images revealed a uniform shape and size distribution of the DNA-NWs, with lengths ranging from 2 to 4 mm and diameters ranging from 150 to 300 nm. One sharp band at the top of the lane(500 bp level) with the loss of electrophoretic mobility during the PAGE(native) gel analysis revealed the successful fabrication of DNA-NWs. The loading efficiency of CPT ranged from 66.85% to 97.35%. MTT and flow cytometry results showed biocompatibility of the blank DNA-NWs even at 95% concentration compared with the CPT-loaded DNANWs. The CPT-loaded DNA-NWs exhibited enhanced apoptosis(22%) compared to the apoptosis(7%)induced by the blank DNA-NWs. The release of CPT from the DNA-NWs was sustained at < 75% for 6 h in the presence of serum, demonstrating suitability for systemic applications. The IC_(50) of CPT@DNA-NWs was reduced to 12.8 nM CPT, as compared with the free CPT solution exhibiting an IC_(50) of 51.2 n *** imaging revealed the targetability, surface binding, and slow internalization of the DNA-NWs in the scavenger-receptor-rich cancer cell line(HepG2) compared with the control cell line.

关键词： Cisplatin(CPT) DNA-nanowires(DNA-NWs) HepG2 resistant cancer cells Scavenger receptors

A DNA-based nanocarrier for efficient cancer therapy

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Journal of Pharmaceutical Analysis 2021年第3期11卷 330-339页

作者： muhammad Abbas mirza muhammad faran ashraf baig Yaliang Zhang Yu-Shun Yang Songyu Wu Yiqiao Hu Zhong-Chang Wang Hai-Liang Zhu State Key Laboratory of Pharmaceutical Biotechnology Nanjing UniversityNanjing210023PR China State Key Laboratory of Analytical Chemistry for Life Sciences School of Chemistry and Chemical EngineeringNanjing UniversityNanjing210023PR China Institute of Drug Research and Development Medical School of Nanjing UniversityNanjing210093PR China

The study aimed to achieve enhanced targeted cytotoxicity and cell-internalization of cisplatin-loaded deoxyribonucleic acid-nanothread(CPT-DNA-NT),mediated by scavenger receptors into HeLa ***-NT was developed with stiff-topology utilizing circular-scaffold to encapsulate *** force microscopy(AFM)characterization of the DNA-NT showed uniformity in the structure with a diameter of 50-150 nm and length of 300-600 *** successful fabrication of the DNA-NT was confirmed through native-polyacrylamide gel electrophoresis analysis,as large the molecular-weight(polymeric)DNA-NT did not split into constituting strands under applied current and *** results of cell viability confirmed that blank DNA-NT had the least cytotoxicity at the highest concentration(512 nM)with a viability of 92%as evidence of its biocompatibility for drug *** assay showed superior cytotoxicity of CPT-DNA-NT than that of the free CPT due to the depot release of CPT after DNA-NT *** DNA-NT exhibited targeted cell internalizations with the controlled intracellular release of CPT(from DNA-NT),as illustrated in confocal ***,in vitro cytotoxicity assessment through flow cytometry showed enhanced apoptosis(72.7%)with CPT-DNA-NT(compared to free CPT;64.4%).CPT-DNA-NT,being poly-anionic,showed enhanced endocytosis via scavenger receptors.

关键词： DNA-nanothread Cisplatin Drug delivery Scavenger receptors Caveolae HeLa cell line

Tylophora hirsuta L. leaf extract attenuates alloxan-induced diabetes in mice by suppressing oxidative stress and α-amylase

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Asian Pacific Journal of Tropical Biomedicine 2021年第9期11卷 394-404页

作者： muhammad Furqan Akhtar Arsalan Shagufta Ammara Saleem mirza muhammad faran ashraf baig Ali Sharif Azhar Rasul Mohamed M.Abdel-Daim Riphah Institute of Pharmaceutical Sciences Riphah International UniversityLahore CampusLahorePakistan Department of Pharmacology Faculty of Pharmaceutical SciencesGovernment College University FaisalabadFaisalabadPakistan Laboratory of Stem Cells Research and Biomedical Engineering for Novel Biofunctional and Pharmaceutical NanomaterialsFaculty of DentistryPrince Philip Dental HospitalThe University of Hong KongHong Kong 999077China Institute of Pharmacy Faculty of Pharmaceutical and Allied Health SciencesLahore College for Women UniversityJail RoadLahorePakistan Department of Zoology Government College University FaisalabadFaisalabadPakistan Pharmacology Department Faculty of Veterinary MedicineSuez Canal UniversityIsmailia 41522Egypt

Objective:To evaluate the antidiabetic potential of leaf extracts of Tylophora hirsuta(***).Methods:The methanolic and ethyl acetate extracts of *** leaves were analyzed by high pressure liquid *** vitro antioxidant activity was determined by ferric ion reduction,1,1-diphenyl-2-picrylhydrazyl,and hydrogen peroxide scavenging *** vitro alpha amylase(α-amylase)inhibitory activity of the plant extracts was *** vivo antidiabetic potential was determined in alloxan-induced diabetic mice to assess glycated hemoglobin(HbA1c),oral glucose tolerance,serum amylase,lipid profile,fasting blood glucose,and body *** lesions of the pancreas,liver and kidney were *** stress biomarkers such as superoxide dismutase,catalase and peroxidase were also ***:Quercetin,chlorogenic acid,p-coumaric acid,and m-coumaric acid were found in the plant *** methanolic plant extract exhibited higher in vitro antioxidant activities than the ethyl acetate ***,methanolic plant extract exhibited(83.90±1.56)%α-amylase inhibitory activity at 3.2 mg/mL *** study showed that the methanolic extract of *** improved the levels of fasting blood glucose,HbA1c,serumα-amylase,lipid profile,liver function biomarkers,and kidney functions of diabetic ***,the methanolic extract ameliorated diabetes-related oxidative stress by increasing superoxide dismutase and catalase activities and decreasing peroxidase and malondialdehyde *** examination showed that the plant extract had improved the integrity of pancreatic islets of Langerhans and reduced the pathological lesions in the liver and kidney of diabetic ***:The methanolic extract of *** exhibits pronounced antidiabetic activity in mice through reduction of oxidative *** plant extract has several natural antioxidants such as phenolic *** extract could serve as a nutra

关键词： Tylophora hirsuta Diabetes mellitus Oxidative stress Alpha-amylase Phenolic compounds Pancreas

DNA Nanotechnology for Modulating the Growth and Development of Neurons

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CCS Chemistry 2021年第9期3卷 2381-2393页

作者： mirza muhammad faran ashraf baig Chunxia Wen Jian Li Xiang Qin Saud Asif Ahmed Xing-Hua Xia State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023 Medical School Nanjing UniversityNanjing 210093

Late prenatal growth,early postnatal growth,and layering of the neocortical neurons(NC-Ns)play determining roles in the development of the cerebral cortex(CC).Here,we systematically explore the interactive role of neuronal surface receptors(NSRs)on cytoskeleton activation(CA)and the piconewton(pN)force generation(P-FG)and their influence on the proper development,growth,and functioning of neurons using a designed DNA nanomechanical device(DNA-NMD).

关键词： neocortical neurons neuronal surface receptors migration of the neuronal endings somal terminal translocation DNA nanomechanical device trispecific activation/deactivation