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Pineapple (<i>Ananas comosus</i>L. Merr.), Waste Streams, Characterisation and Valorisation: An Overview

Open Journal of Ecology 2021年第9期11卷 610-634页

作者： Wafaa M. Hikal Abeer A. Mahmoud Hussein A. H. Said-Al Ahl Amra Bratovcic Kirill G. Tkachenko miroslava Kačániová Ronald Maldonado Rodriguez Department of Biology Faculty of Science University of Tabuk Tabuk Saudi Arabia Water Pollution Research Department Environmental Research Division National Research Centre 33 El-Bohouth St. Dokki Giza Egypt Department of Botany (Plant Physiology Section) Faculty of Agriculture Cairo University Giza Egypt Medicinal and Aromatic Plants Research Department National Research Centre 33 El-Bohouth St. Dokki Giza Egypt Department of Physical Chemistry and Electrochemistry Faculty of Technology University of Tuzla Tuzla Bosnia and Herzegovina V. L. Komarov Botanical Institute of the Russian Academy of Sciences Saint Petersburg Russia Department of Fruit Science Viticulture and Enology Faculty of Horticulture and Landscape Engineering Slovak University of Agriculture Nitra Slovakia Department of Bioenergetics Food Analysis and Microbiology Institute of Food Technology and Nutrition University of Rzeszow Cwiklinskiej 1 Rzeszow Poland The Fluoromatics Laboratory 10 Rue du Vieux-Moulin 1213 Onex Geneva Switzerland

Processing pineapple industry produces huge amounts of waste thus contributing to worsen the global environmental problem. Valorising pineapple waste through further processing until it is transformed into valuable products using environmentally friendly techniques is both, a challenge, and an opportunity. The aim of this review is to characterize and highlight the phytochemical constituents of pineapple peel, their biological activity, and to evaluate the current state-of-art for the utilization of pineapple waste from the processing industry for obtaining pharmaceuticals, food, and beverages, biocombustibles, biodegradable fibers, and other different usage. Pineapple residues are rich in many bioactive compounds such as ferulic acid, vitamin A and C as antioxidant, and containing alkaloids, flavonoids, saponins, tannins, cardiac glycoside, steroids, triterpenoids and phytosterols may provide a good source of several beneficial properties, as well as bromelain that showed significant anticancer activity. Also, pineapple processing residues contain important volatile compounds used as aroma enhancing products and have high potential to produce value-added natural essences. Pineapple peels can be used as nonpharmacological therapeutical in the form of processed food and instant drinks;its potent natural antimicrobial properties may be applied for food conservation and as potential leads to discover new drugs to control some infectious microbial. Pineapple waste is a promising source of metabolites for therapeutics, functional foods, and cosmeceutical applications.

关键词： Pineapple Peel Ananas comosus Phytochemical Constituents Antioxidant Activity Anticancer Antimicrobial Activities

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Investigation of Interaction of Some Chalcones and Cyclic Chalcone Analogues with Outer Mitochondrial Membrane by UV-VIS and Fluorescence Spectroscopy

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Spectral Analysis Review 2013年第1期1卷 1-9页

作者： Vladimíra Tomecková miroslava Stefanisinová Beáta Veliká Krisztina Fodor Pal Perjési Marek Stupák Juraj Guzy Stefan Tóth Jr Tímea Pekárová Department of Medical Chemistry Biochemistry and Clinical BiochemistryFaculty of MedicinePavol Jozef Safárik University in KosiceKosiceSlovakia Institute of Pharmaceutical Chemistry Faculty of MedicineUniversity of PécsPécsHungary

Interaction of the synthetic chalcones (1b,1c) and their cyclic analogues (2b,2c) with bovine (BSA) and human serum albumin (HSA) as well as with rat liver mitochondria (RLM) was studied by fluorescence spectroscopy. The maxima of emission fluorescence spectra were changed only in the case of 2b and 2c during interaction with BSA, HSA as well as mitochondrial outer membrane showing a slight hypsochromic shift and decrease of fluorescence. Interaction of the methoxy-(1b,2b) and the dimethylamino-substituted (1c,2c) compounds with outer mitochondrial membrane were studied by fluorescence polarization. Fluorescence polarization of 1b in the presence of the two proteins and mitochondria was found to be unchanged. Under similar conditions (2b,1c,2c) showed continuously increasing fluorescence polarization signal during the 30 minute period of investigations. Since fluorescence polarization supposes that as a result of binding these substances to proteins and lipids. Compound 2c displayed a continuous increase of fluorescence polarization signal in the presence of proteins (BSA, HSA), yeast cytoplasm (YC) and mitochondria (YM and RLM). This compound displayed a significant cytotoxic effect. This pattern of interaction with proteins might be one of the contributing vectors of the observed cytotoxicity against several human carcinoma cell lines.

关键词： Chalcones Yeast Cytoplasm Yeast Mitochondria Rat Liver Mitochondria Fluorescence Polarization? Fluorescence Synchronous Fingerprint

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仅在永久性脑缺血后短暂出现的星形胶质细胞样NG2胶质细胞亚群

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神经损伤与功能重建 2021年第8期16卷 F0003-F0003页

作者： Denisa Kirdajova Lukas Valihrach Martin Valny Jan Kriska Daniela Krocianova Sarka Benesova Pavel Abaffy Daniel Zucha Ruslan Klassen Denisa Kolenicova Pavel Honsa Mikael Kubista miroslava Anderova 杜一星(编译) Department of Cellular Neurophysiology Institute of Experimental MedicineCzech Academy of SciencesPragueCzech Republic Second Faculty of Medicine Charles UniversityCzech Republic Laboratory of Gene Expression Institute of Biotechnology CASBIOCEVVestecCzech Republic Faculty of Chemical Technology Laboratory of Informatics and ChemistryUniversity of Chemistry and TechnologyPragueCzech Republic Faculty of Science Charles UniversityPragueCzech Republic 不详

NG2神经胶质细胞在生理和病理条件下显示出较强的增殖和分化潜力。本研究用3月龄Cspg4启动子下由tdTomato标记NG2神经胶质细胞的小鼠,在不同类型的脑疾病模型(局灶性脑缺血、皮质刺伤和脱髓鞘)中研究NG2细胞的增殖和分化潜力,研究首先... 详细信息

NG2神经胶质细胞在生理和病理条件下显示出较强的增殖和分化潜力。本研究用3月龄Cspg4启动子下由tdTomato标记NG2神经胶质细胞的小鼠,在不同类型的脑疾病模型(局灶性脑缺血、皮质刺伤和脱髓鞘)中研究NG2细胞的增殖和分化潜力,研究首先采用单细胞RT-qPCR和Kohonen自组织映射图分析从未损伤和特定损伤后的皮质/胼胝体分离的tdTomato阳性细胞,比较不同损伤后NG2神经胶质细胞表达概况。这种方法使我们能够区分NG2神经胶质细胞和少突胶质细胞,每个细胞群都包含4个不同的亚群。基因表达谱显示,表达GFAP(反应性星形胶质细胞的标志物)的NG2胶质细胞亚群仅在局灶性脑缺血后短暂出现。然而,在较轻的损伤后(皮质刺伤和脱髓鞘),反映少突胶质细胞成熟不同阶段的亚群明显占优势。免疫组织化学也证实了不同亚群的损伤依赖性发生率。本研究使用单细胞RNA测序分析这种独特的短暂星形胶质细胞样NG2胶质细胞亚群,并采用膜片钳技术揭示了其基本的细胞膜特性。综上所述,我们证明了星形胶质细胞样NG2神经胶质细胞是仅在局灶性脑缺血后短暂出现的NG2神经胶质细胞的特定亚群。这些位于缺血后神经胶质瘢痕中的细胞处于活跃的细胞周期,并显示出与皮质星形胶质细胞相似的电流模式,可能是缺血后胶质瘢痕形成和修复过程中的重要参与者。

关键词：星形胶质细胞脱髓鞘缺血 NG2胶质细胞少突胶质细胞刺伤

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