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A midgut-specific lytic polysaccharide monooxygenase of Locusta migratoria is indispensable for the deconstruction of the peritrophic matrix

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Insect Science 2022年第5期29卷 1287-1298页

作者： ming-bo qu Xiao-Xi Guo Lin Kong Ling-Jie Hou Qing Yang School of Bioengineering Dalian University of TechnologyDalianLiaoning ProvinceChina State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijingChina Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenGuangdong ProvinceChina

Lytic polysaccharide monooxygenases(LPMOs)are important enzymes that boost the hydrolysis of recalcitrant polysaccharides,such as *** are found extensively in different insect species and are classified as auxiliary activities family 15(AA15)LPMOs(LPMO15).Some of them were identified from the insect midgut and proven to act on ***,knowledge about their physiological roles during insect growth and development remains ***,we found that midgut-specific LPMO15s are widely distributed in different insect orders,such as the orthopteran Locusta migratoria and the lepidopteran bombyx *** *** as a model insect,the function of midgut-specific LmLPMO15-3 during development was ***-stranded RNA-mediated downregulation of LmLPMO15-3 expression at the 4th or 5th instar nymph stage severely decreased the survival rate and resulted in lethal *** and eosin staining results indicated that the deficient individuals exhibited incompletely digested peritrophic matrix(PM),which suggested that LmLPMO15-3 is essential for the deconstruction of the PM during *** study provides direct evidence of the physiological importance of a midgut-specific LPMO15 during insect *** *** is one of the most destructive agricultural pests,LmLPMO15-3 is a potential target for pest management.

关键词： chitin Locusta migratoria lytic polysaccharide monooxygenase midgut peritrophic matrix RNAi

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Insect group II chitinase OfChtll promotes chitin degradation during larva-pupa molting

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Insect Science 2021年第3期28卷 692-704页

作者： ming-bo qu Shao-Peng Sun Yuan-Sheng Liu Xiao-Rui Deng Jun Yang Qing Yang School of Bioengineering Dalian University of TechnologyDalianLiaoningChina State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection and Shenzhen Agricultural Genome Research InstituteChinese Academy of Agricultural SciencesBeijingChina

The insect group ll chitinase(ChtIl,also known as Cht10)is a unique chitinasewith multiple catalytic and chitin-binding *** has been proven genetically to be anessential chitinase for ***,Chtll's role in chitin degradation during insectdevelopment remains poorly *** this knowledge is the key to fullyunderstanding the chitin degradation system in ***,we investigated the roleof OfChtll during the molting of Ostrinia furnacalis,a model lepidopteran pest *** was expressed earlier than OfChtI(OfCht5)and OfChi-h,at both the gene andprotein levels during larva-pupa molting as evidenced by quantitative polymerase chainreaction and western blot analyses.A truncated OfChtII,OfChtII-B4C1,was recombinantlyexpressed in Pichia pastoris cells and purified to *** recombinant OfChtll-B4C1 loosened compacted chitin particles and produced holes in the cuticle surface asevidenced by scanning electron *** synergized with OfChtl and OfChi-h whenhydrolyzing insoluble *** findings suggested an important role for ChtIl duringinsect molting and also provided a strategy for the coordinatdd degradation of cuticularchitin during insect molting by Chtll,Chtl and Chi-h.

关键词： chitin chitinase chitin binding degradation multiple module synergy

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Dynamic Regulation of Hydrogen bonding Networks and Solvation Structures for Synergistic Solar‑Thermal Desalination of Seawater and Catalytic Degradation of Organic Pollutants

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Nano-Micro Letters 2025年第2期17卷 548-565页

作者： ming‑Yuan Yu Jing Wu Guang Yin Fan‑Zhen Jiao Zhong‑Zhen Yu Jin qu Beijing Key Laboratory of Advanced Functional Polymer Composites Beijing University of Chemical TechnologyBeijing 100029People’s Republic of China State Key Laboratory of Organic‑Inorganic Composites College of Materials Science and EngineeringBeijing University of Chemical TechnologyBeijing 100029People’s Republic of China

Although solar steam generation strategy is efficient in desalinating seawater,it is still challenging to achieve continuous solar-thermal desalination of seawater and catalytic degradation of organic ***,dynamic regulations of hydrogen bonding networks and solvation structures are realized by designing an asymmetric bilayer membrane consisting of a bacterial cellulose/carbon nanotube/Co_(2)(OH)_(2)CO_(3)nanorod top layer and a bacterial cellulose/Co_(2)(OH)_(2)CO_(3)nanorod(BCH)bottom ***,the hydrogen bonding networks inside the membrane can be tuned by the rich surface–OH groups of the bacterial cellulose and Co_(2)(OH)_(2)CO_(3)as well as the ions and radicals in situ generated during the catalysis ***,both SO_(4)^(2−)and HSO_(5)−can regulate the solvation structure of Na^(+)and be adsorbed more preferentially on the evaporation surface than Cl^(−),thus hindering the de-solvation of the solvated Na^(+)and subsequent nucleation/growth of ***,the heat generated by the solar-thermal energy conversion can accelerate the reaction kinetics and enhance the catalytic degradation *** work provides a flow-bed water purification system with an asymmetric solar-thermal and catalytic membrane for synergistic solar thermal desalination of seawater/brine and catalytic degradation of organic pollutants.

关键词： Solar steam generation Seawater desalination Catalytic degradation Bacterial cellulose Cobalt hydroxycarbonate nanorods

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940nm高功率半导体激光器研究

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中国激光 2004年第5期31卷 535-537页

作者：万春明薄报学曲轶姜会林长春理工大学高功率半导体激光国家重点实验室吉林长春130022

报道了一种采用大光学腔结构的InGaAs/GaAs/AlGaAs应变量子阱高功率半导体激光器。在量子阱能级本征值方程的数值求解基础上 ,优化了InGaAs阱层材料的In组份含量 ;采用大光学腔结构以有效降低垂直于结平面方向的光束发散角及腔面的光功... 详细信息

报道了一种采用大光学腔结构的InGaAs/GaAs/AlGaAs应变量子阱高功率半导体激光器。在量子阱能级本征值方程的数值求解基础上 ,优化了InGaAs阱层材料的In组份含量 ;采用大光学腔结构以有效降低垂直于结平面方向的光束发散角及腔面的光功率密度 ,实现器件的高功率、低发散角光。设计的激光器外延结构采用分子束外延 (MBE)方法生长 ,成功获得具有较低激射阈值的 94 0nm波长激光器外延片。对 10 0 μm条形 ,10 0 0 μm腔长的制备器件测试表明 ,器件的最大连续输出功率达到 2W ,峰值波长为 939.4nm ,远场水平发散角为 10° ,垂直发散角为 30°。器件的阈值电流为 30 0mA。

关键词：激光技术高功率分子束外延半导体激光器

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Chondroitinase ABC combined with Schwann cell transplantation enhances restoration of neural connection and functional recovery following acute and chronic spinal cord injury

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Neural Regeneration Research 2025年第5期20卷 1467-1482页

作者： Wenrui qu Xiangbing Wu Wei Wu Ying Wang Yan Sun Lingxiao Deng Melissa Walker Chen Chen Heqiao Dai Qi Han Ying Ding Yongzhi Xia George Smith Rui Li Nai-Kui Liu Xiao-ming Xu Spinal Cord and Brain Injury Research Group Stark Neurosciences Research InstituteDepartment of Neurological SurgeryIndiana University School of MedicineIndianapolisINUSA Department of Hand Surgery the Second Hospital of Jilin UniversityChangchunJilin ProvinceChina Shriners Hospitals Pediatric Research Center Temple University School of MedicinePhiladelphiaPAUSA Department of Anatomy&Cell Biology Indiana University School of MedicineIndianapolisINUSA

Schwann cell transplantation is considered one of the most promising cell-based therapy to repair injured spinal cord due to its unique growth-promoting and myelin-forming properties.A the Food and Drug Administration-approved Phase I clinical trial has been conducted to evaluate the safety of transplanted human autologous Schwann cells to treat patients with spinal cord injury.A major challenge for Schwann cell transplantation is that grafted Schwann cells are confined within the lesion cavity,and they do not migrate into the host environment due to the inhibitory barrier formed by injury-induced glial scar,thus limiting axonal reentry into the host spinal *** we introduce a combinatorial strategy by suppressing the inhibitory extracellular environment with injection of lentivirus-mediated transfection of chondroitinase ABC gene at the rostral and caudal borders of the lesion site and simultaneously leveraging the repair capacity of transplanted Schwann cells in adult rats following a mid-thoracic contusive spinal cord *** report that when the glial scar was degraded by chondroitinase ABC at the rostral and caudal lesion borders,Schwann cells migrated for considerable distances in both rostral and caudal *** Schwann cell migration led to enhanced axonal regrowth,including the serotonergic and dopaminergic axons originating from supraspinal regions,and promoted recovery of locomotor and urinary bladder ***,the Schwann cell survival and axonal regrowth persisted up to 6 months after the injury,even when treatment was delayed for 3 months to mimic chronic spinal cord *** findings collectively show promising evidence for a combinatorial strategy with chondroitinase ABC and Schwann cells in promoting remodeling and recovery of function following spinal cord injury.

关键词： axonal regrowth bladder function chondroitinase ABC functional recovery glial scar lentivirus migration Schwann cell spinal cord injury transplantation

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椎体骨皮质强化差异对骨质疏松性椎体压缩性骨折生物力学的影响

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中国组织工程研究 2025年第21期29卷 4430-4438页

作者：方伟黄兴桦屈波杨红胜重庆市云阳县中医院骨伤科重庆市404500 成都医学院附属第一医院骨科四川省成都市610500

背景:骨质疏松性椎体压缩性骨折经皮椎体成形过程中骨水泥在椎体内的骨皮质强化区域对脊柱生物力学及临床疗效有重要影响,但以往研究大多局限于二维层面。目的:采用有限元分析法探讨经皮椎体成形过程中骨水泥在椎体三维层面内骨皮质强... 详细信息

背景:骨质疏松性椎体压缩性骨折经皮椎体成形过程中骨水泥在椎体内的骨皮质强化区域对脊柱生物力学及临床疗效有重要影响,但以往研究大多局限于二维层面。目的:采用有限元分析法探讨经皮椎体成形过程中骨水泥在椎体三维层面内骨皮质强化差异对骨质疏松性椎体压缩性骨折椎体、邻近椎间盘及终板生物力学分布的影响,以评估其效果。方法:建立T12椎体骨质疏松性压缩性骨折行经皮椎体成形治疗的有限元模型,从横断面、矢状面、冠状面对骨水泥有无皮质强化情况进行分组分析,研究不同体位变化下,骨皮质强化对T12椎体松质骨、皮质骨、T11/T12椎间盘、T12/L1椎间盘、T11下终板、L1上终板生物力学的影响。结果与结论:(1)在垂直压缩力作用下,经皮椎体成形过程中有或无骨水泥皮质强化在不同体位变化下除伤椎皮质骨以外结构应力变化均不明显;(2)伤椎皮质骨Von Mises应力值在人体前屈、左/右侧弯和左/右轴向旋转时差异显著,同一平面内骨皮质完全强化组较无骨皮质强化组最大Von Mises应力值明显变小,随着骨皮质强化越广泛Von Mises应力值整体呈下降趋势;(3)提示行经皮椎体成形治疗时,横断面内骨水泥应尽量广泛对称水平分布于椎体骨皮质边缘,矢状面内骨水泥应广泛纵向分布且靠近上下终板及前后壁,冠状面内骨水泥应广泛分布于中线两侧同时对称接触上下终板及侧壁,可有效避免伤椎再骨折风险且不增加邻椎骨折、残留盘源性疼痛风险。

关键词：经皮椎体成形骨皮质强化骨质疏松性椎体压缩性骨折邻近椎间盘终板三维有限元分析生物力学

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Analysis of Seasonal Differences of Chlorophyll,Dimethylsulfide,and Ice Between the Greenland Sea and the Barents Sea

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Journal of Ocean University of China 2023年第6期22卷 1592-1604页

作者： ZHONG Guangsheng qu bo School of Science Nantong University226019China

Arctic Ocean(AO)climate is closely related to sea ice concentration(ICE)and chlorophyll_a(CHL)*** 2003–2014,the spatial average concentrations of CHL,ICE,sea surface temperature(SST),wind speed(WIND)in the Greenland Sea region(GS)(20˚W–10˚E,70˚–80˚N)and the Barents Sea region(BS)(30˚–50˚E,70˚–80˚N)are analysed and *** CHL was observed in BS,about 60%higher than that in *** with the northern regions of BS and GS(BSN and GSN),CHL in the southern region of BS and GS(BSS and GSS)increased by 77%and 42%*** ice melting in BSN is the main reason for phytoplankton *** 2010,there was an unusual peak of CHL concentration in *** sea-sonal peaks of CHL appeared two weeks earlier in BS than in *** earlier and more extensive ice melting and the persistent nega-tive North Atlantic Oscillation(NAO)index may be the reasons for higher CHL blooms in *** spatial average ICE concentra-tion of BS in BSN and BSS is 27%and 1.2%*** NAO in the previous winter may lead to an increase in ICE in *** has a great influence on CHL and ICE in *** melting is positively correlated with CHL,especially in GS in recent decades,CHL has a significant positive correlation with surface mass concentration of dimethylsulfide(DMS),especially in *** an indicator of Arctic warming,BS needs more attention from Arctic researchers.

关键词： dimethylsulfide chlorophyll ice North Atlantic Oscillation Arctic

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Vertical Chlorophyll and Dimethylsulfide Profile Simulations in Southern Greenland Sea

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Journal of Ocean University of China 2022年第6期21卷 1565-1574页

作者： qu bo SUN Wenjing School of Science Nantong UniversityNantong 226007China YK Pao School Middle School CampusShanghai 200336China

Biogenetic sulfide dimethylsulfide(DMS)plays a major role on the global climate,especially in Arctic *** simulate DMS concentration is an important *** we introduced both biogeochemical depth-averaged model G93 and its extension model-one dimensional DMS *** surface concentrations,vertical profiles of chlorophyll(CHL)and DMS are simulated using the two models within southern Greenland Sea(0°E–10°E,70°N–75°N)during year *** the input data for the models simulations,the spatial monthly mean of methodology forcings including sea surface temperature(SST),wind speed(WIND),cloud cover(CLD),sea ice concentration(ICE)and mixed layer depth(MLD)are *** 8-day time series of chlorophyll-a(CHL)are used as observation data for CHL related parameter ***ó’s imperial formula is used as the monthly DMS observation *** Genetic Algorithm technique is used for the parameter *** simulation results show that the most DMS related surface concentrations exhibit the normal distributions with peak during ***,DMS and DMSP(dimethylsulphoniopropionate)vertical profiles are obtained for July,August and September in year *** had the higher variation of subsurface concentration maximum(SCM)in July with the lower surface concentration *** had surface higher and subsurface lower profile for the all three *** also had subsurface high in *** SCM CHL diurnal variation in the subsurface also can be resulted from diurnal changes in MLD and vertical mixing variations,plus photolysis and wind-driven ventilations.

关键词： dimethylsulfide vertical profile chlorophyll biogeochemical modeling Greenland Sea

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Microstructure and thermal properties of copper matrix composites reinforced with titanium-coated graphite fibers

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Rare Metals 2013年第1期32卷 75-80页

作者： Hao-ming Zhang Xin-bo He Xuan-Hui qu Qian Liu Xiao-Yu Shen School of Materials Science and Engineering University of Science and Technology Beijing State Key Laboratory for Advanced Metals and Materials University of Science and Technology Beijing

Milled form of mesophase pitch-based graphite fibers were coated with a titanium layer using chemical vapor deposition technique and Ti-coated graphite fiber/Cu composites were fabricated by hot-pressing sintering. The composites were characterized with X-ray diffraction, scanning/transmission electron microscopies, and by mea- suring thermal properties, including thermal conductivity and coefficient of thermal expansion （CTE）. The results show that the milled fibers are preferentially oriented in a plane perpendicular to the pressing direction, leading to anisotropic thermal properties of the composites. The Ti coating reacted with graphite fiber and formed a continuous and uniform TiC layer. This carbide layer establishes a good metallurgical interracial bonding in the composites, which can improve the thermal properties effectively. When the fiber content ranges from 35 vol% to 50 vol%, the in-plane thermal conductivities of the composites increase from 383 to 407 W.（m.K）-~, and the in-plane CTEs decrease from 9.5 x 10-6 to 6.3 10-6 K-1.

关键词： Metal matrix composites Titanium coating Microstructure Thermal conductivity Coefficient of thermal expansion

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实验性热损伤所致的兔耳中央动脉血管平滑肌细胞增生

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实用预防医学 2005年第2期12卷 270-272页

作者：赵爱明屈波陈北阳李迎秋湖南中医学院组胚教研室湖南长沙410007

目的　探索建立热刺激动脉诱发血管平滑肌细胞增生的动物模型。　方法　兔耳中央动脉中间2cm处被热水(4 3±0 .5 )℃刺激,每天一次,每次3h ,共做3次。分别于造模后3d、1周、2周、4周时取材,石蜡切片。光学显微镜及图象分析仪进行形态学... 详细信息

目的　探索建立热刺激动脉诱发血管平滑肌细胞增生的动物模型。　方法　兔耳中央动脉中间2cm处被热水(4 3±0 .5 )℃刺激,每天一次,每次3h ,共做3次。分别于造模后3d、1周、2周、4周时取材,石蜡切片。光学显微镜及图象分析仪进行形态学观察。　结果　中膜成分增生并突入血管腔内,造成管腔变狭。造模4周时管腔面积与造模3d时、造模7d时相比明显缩小(2 .5 8±0 .3 5 )mm2 /10 0比(3 .2 5±0 .5 9)mm2 /10 0、(3 .14±0 .2 4)mm2 /10 0 ,P <0 .0 5。造模4周时中膜厚度及平滑肌细胞层数与造模3d时、造模7d时相比明显增加(10 0 .5±15 .5 ) μm比(69.2±16.1) μm、(71.4±16.3 ) μm ,P <0 .0 5 ;(11.6±0 .8)层比(7.2±1.1)层、(6.5±1.7)层,P <0 .0 5。　结论　血管外热刺激可以诱发血管平滑肌细胞增生。该模型简便易行、重复性好。

关键词：热损伤血管平滑肌细胞增生兔耳中央动脉动物模型

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