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Affinity Switching for Lysozyme and Dual-responsive Microgels by Stopped-flow Technique： Kinetic Control and Activity Evaluation

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Chinese Journal of Polymer Science 2017年第8期35卷 950-960页

作者： yun-zhu wu zhi-huang zhang xin han jian zhang wen-ming zhang 殷俊 Department of Polymer Science and Engineering School of Chemistry and Chemical Engineering Hefei University of Technology and Anhui Key Laboratory of Advanced Functional Materials and Devices Hefei 230009 China

The use of proteins as therapeutics in nanomedicine is an emerging research field and has developed ***,proteins are always vulnerable to renal excretion or digestion by the proteolytic system in vivo,which limits their usage to a large *** biocompatible polymers have been covalently linked to proteins to protect them from recognition by the immune system and prolong their circulation time,the biological activity of them is sometimes *** fill this gap,physical isolation,wrapping,or encapsulation techniques are *** to now,various mature examples were reported,but the whole time scales for guest molecules loading and releasing,especially the initial rapid loading process,were rarely ***,a series of dual-responsive poly（N-isopropylacrylamide-co-methacrylic acid）（P（NIPAM-co-MAA）） microgels were synthesized and employed to investigate the kinetics of in situ complexation and release of lysozyme under external stimuli modulation upon a stopped-flow apparatus,which was suitable for rapid dynamic *** inspection of the adsorption kinetics during the early stages（〈 50 s） revealed that the initial microgel collapse occurred within ~1 s,with more rapid transitions being observed when higher lysozyme concentrations were *** the dynamic traces could be well fitted with a double exponential function,suggesting a fast（τ1） and a slow（τ2） relaxation time,***,the kinetics of releasing bound lysozyme from microgels was carried on by utilizing the p H-responsive property,and the evaluation of the activity of released lysozyme was synchronously measured in a Micrococcus lysodeikticus（***） cell *** corresponding relaxation time（τ） was also calculated by fitting the recorded dynamic *** speculate that this work can provide basic dynamics data and theoretical basis for microgels based nanocarriers to be used for protein delivery,controlled release,and possible chemical sepa

关键词： responsive relaxation traces encapsulation lysozyme inspection Micrococcus fitting utilizing kinetics

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Impact of atmospheric ionization by delayed radiation from highaltitude nuclear explosions on radio communication

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Nuclear Science and Techniques 2019年第12期30卷 58-64页

作者： Heng Xu jian-ming Ouyang Shang-wu Wang Yun Liu Xu Sun College of Liberal Arts and Sciences National University of Defense TechnologyChangsha 410073China College of Physics and Micro Electronic Hunan UniversityChangsha 410006China College of Electronics Science National University of Defense TechnologyChangsha 410073China

In this study,we investigated the motion,shape,and delayed radiation intensity of a radioactive cloud by establishing a volume-source model of delayed radiation after high-altitude nuclear ***,the spatial distribution of electron number density at different moments on the north side of the explosion point generated by delayed γ-rays and delayed β-rays from the radioactive cloud under the influence of the geomagnetic field was calculated by solving chemical reaction kinetics *** impact of radio communication in the different frequency bands on the process of atmospheric ionization was also *** numerical results of the high-altitude nuclear explosion (120 km high and with a 1 megaton equivalent at 40°N latitude) indicated that the peak of electron number density ionized delayed γ-rays is located at a height of approximately 100 km and that of electron number density ionized delayed β-rays is about 90 km *** 1 min of explosion,the radio communication in the medium frequency (MF) and high-frequency (HF)bands was completely interrupted,and the energy attenuation of the radio wave in the very high-frequency (VHF)band was extremely *** minutes later,the VHF radio communication was basically restored,but the energy attenuation in the HF band was still *** 30 min,theVHF radio communication returned to normal,but its influence on the HF and MF radio communication continued.

关键词： High-altitude nuclear explosions Delayed radiation Ionization effect Radio communication

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Profile Analysis of the Proteome of the Egg of the High Royal Jelly Producing Bees (Apis mellifera L.)

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Agricultural Sciences in China 2007年第9期6卷 1138-1148页

作者： ZHANG Ean LI jian-ke wu Li-ming Department of Bioengineering Zhengzhou University Zhengzhou 450001 P.R.China Institute of Apicultural Research Chinese Academy of Agricultural Sciences Beijing 100093 P.R.China

The protein composition of the egg development in the high royal jelly producing bees （Apis mellifera L.） was investigated. This pioneer study was to separate and quantify the proteins in the egg of the high royal jelly producing worker bees （Apis mellifera L.） by using two-dimensional gel electrophoresis along with their three-day development. The results showed that 160, 195, and 176 proteins, with a wide range of molecular weight （17-80 KDa） and relatively narrow scope of pI （4. 00-8.40） could be detected on day 1, day 2, and day 3, respectively, during the developmental process of the egg. Meanwhile 44 protein spots were constantly detected along with the egg development. Among them 36% were in the uptrend along with the egg development, 14% were in the downtrend, and 39% were of the largest expressed volume on day 2. In addition, the specific proteins were expressed on day 1, day 2, and day 3 （89, 77, and 80, respectively）. Besides the coexistent and specific proteins, 24 proteins were expressed on day 1 and day 2, but silenced on day 3, 49 proteins were expressed on day 2 and day 3, but silenced on day 1, only 3 proteins were expressed on day 1 and day 3, but silenced on day 2. The result indicates that egg development is a sequential and complex gene controlled process, where the eggs of day 2 express the most active proteins. The coexistent proteins suggest that it is conservative and indispensable for this event. These specific proteins suggest that the different developmental stage needs specific proteins to regulate it.

关键词： honeybees high royal jelly producing worker bee's egg two-dimensional gel electrophoresis proteome

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High-Speed Ring Shear Tests to Study the Motion and Acceleration Processes of the Yingong Landslide

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Journal of Mountain Science 2015年第6期12卷 1534-1541页

作者： HU ming-jian Pan Hua-li Zhu Chang-qi Wang Fa-wu State Key Laboratory of Geomechanics and Geotechnical Engineering Institute of Rock and Soil Mechanics Chinese Academy of Sciences Key Laboratory of Mountain Hazards and Earth Surface Process Institute of Mountain Hazards and Environment Chinese Academy of Sciences Research Center on Natural Disaster Reduction Shimane University

In this paper, the motion and acceleration process, as well as the mechanism of a high speed and long run landslide are investigated by adopting high speed ring shear test and taking the landslide occurred at Yigong River in Bomi, Tibet on April 9, 2000 as the background. According to the motion characteristics of high-speed and long distance motion landside, the mechanism is studied under different conditions such as shear speed, consolidated drained and consolidated undrained status. Results show that high speed shearing process hinders and delays the dissipation of pore pressure, and drives pore water migrating to shear zone slowly. Both of water content and fine particle content at shear zone are obviously higher than those in other layers; and soil liquefaction occurs at shear zone in the saturated consolidated undrained ring shear tests. The effective internal friction angle of the consolidated undrained soil is much lower than that of the consolidated drained soil under ring shearing. The results also indicate that the shearing speed affecting the strength of soil to some extent. The higher the ring shearing speed is, the lower the strength of soil is. This investigation provides a preliminary interpretation of the mechanism of the motion and acceleration process of the Yigong landslide, occurred in Tibet in 2000.

关键词： Yigong landslide Ring shear tests Shear zone Liquefaction Strength

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甘肃武都赵家庄地区水系沉积物地球化学特征及评价

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甘肃地质 2016年第3期25卷 64-69页

作者：苟学明巫建华冯伟宋宪生陈云杰东华理工大学江西南昌330000 核工业二0三研究所陕西咸阳712000

赵家庄地区位于南秦岭加里东—印支褶皱带南缘,文县—康县—略阳断裂带北侧,属迭部—武都成矿带东段。笔者从区域地质背景和矿区地质特征入手,重点分析了赵家庄地区的水系沉积物金异常晕,表明该区水系沉积物晕分布与构造展布方向一致,... 详细信息

赵家庄地区位于南秦岭加里东—印支褶皱带南缘,文县—康县—略阳断裂带北侧,属迭部—武都成矿带东段。笔者从区域地质背景和矿区地质特征入手,重点分析了赵家庄地区的水系沉积物金异常晕,表明该区水系沉积物晕分布与构造展布方向一致,金异常晕对地层的选择性明显;通过对水系沉积物测量中Au、Ag、As、Cu、Zn、Mo、Pb、W、Sb、Bi、Hg等11种元素指标进行R型聚类分析和相关性分析,得出Au和As的相关性最好,Mo与Zn、Bi与Pb相关性密切,Sb、Cu、Ag、相关性较好;而Hg与其它元素相关性不显著。区内可选用Au,As,Mo,Zn 4种元素做为代表性指标进行综合分析成矿预测。

关键词：地球化学水系沉积物聚类分析找矿标志甘肃武都

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Analysis of Non-WSSUS Fading Dispersive Channel Model

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The Journal of China Universities of Posts and Telecommunications 2006年第2期13卷 20-25页

作者： ZHANG ming ZHANG jian-hua jianG Jun ZHANG Ping wu Yu-fei Wireless Technology Innovation Labs (WTI) Beijing University of Posts and TelecommunieationsBeijing 100876 P.R. China Motorola (China)Electronics Ltd. No. 4 Avenue Technology Development Zone Tianjin 301800 P.R. China

The Wide-Sense Stationary Uncorrelated Scattering （WSSUS） model has long been viewed as a basic channel model to describe the fading dispersive channel. But non- WSSUS models have more universal applicability when the wireless mobile channel with broadband is studied with finer and more detailed knowledge of propagation environments. So the four-Dimension （4-D） characteristics of channel, namely time, lag, frequency, and Doppler, should be studied together. In this paper, Wigner-Ville distribution of Time-Frequency （TF） domain is introduced to analyze channel in which the incidence rays are non-stationary and correlated with each other. Several channel models, according to different move modes of incidence rays, with time-varying Doppler shift are designed and 4-D Local Scattering Function （LSF） are computed and simulated respectively. Our simulation results show the LSF present asymmetric and non-periodic TF distri- bution for some symmetric and periodic move modes of incidence rays.

关键词： non- WSSUS channel stimulation local scattering function Wigner- Ville distribution Doppler shift

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Massive GGAAs in genomic repetitive sequences serve as a nuclear reservoir of NF-κB

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Journal of Genetics and Genomics 2018年第4期45卷 193-203页

作者： jian wu Qiao Wang Wei Dai Wei Wang ming Yue Jinke Wang State Key Laboratory of Bioelectronics Southeast University Department of Infectious Diseases The First Affiliated Hospital of Nanjing Medical University

Nuclear factor kB（NF-kB） is a DNA-binding transcription factor. Characterizing its genomic binding sites is crucial for understanding its gene regulatory function and mechanism in cells. This study characterized the binding sites of NF-kB ReIA/p65 in the tumor neurosis factor-a（TNFa） stimulated HeLa cells by a precise chromatin immunoprecipitation-sequencing（ChIP-seq）. The results revealed that NF-kB binds nontraditional motifs（nt-motifs） containing conserved GGAA quadruplet. Moreover, nt-motifs mainly distribute in the peaks nearby centromeres that contain a larger number of repetitive elements such as satellite, simple repeats and short interspersed nuclear elements（SINEs）. This intracellular binding pattern was then confirmed by the in vitro detection, indicating that NF-kB dimers can bind the nontraditional kB（nt-KB） sites with low affinity. However, this binding hardly activates *** study thus deduced that NF-kB binding nt-motifs may realize functions other than gene regulation as NF-kB binding traditional motifs（t-motifs）. To testify the deduction, many ChIP-seq data of other cell lines were then analyzed. The results indicate that NF-kB binding nt-motifs is also widely present in other cells. The ChIP-seq data analysis also revealed that nt-motifs more widely distribute in the peaks with low-fold enrichment. Importantly, it was also found that NF-kB binding nt-motifs is mainly present in the resting cells, whereas NF-kB binding t-motifs is mainly present in the stimulated cells. Aston？ishingly, no known function was enriched by the gene annotation of nt-motif peaks. Based on these results, this study proposed that the nt-KB sites that extensively distribute in larger numbers of repeat elements function as a nuclear reservoir of NF-kB. The nuclear NF-kB proteins stored at nt-KB sites in the resting cells may be recruited to the t-KB sites for regulating its target genes upon stimulation.

关键词： NF-κB Binding region GGAA Storage

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Research on shape optimization of CSG dams

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Water Science and Engineering 2011年第4期4卷 445-454页

作者： Xin CAI Ying-li wu jian-gang YI Yu ming College of Mechanics and Materials Hohai University Naijing 210098 P R. China College of Water Conservancy and Hydropower Engineering Hohai University Naijing 210098 P. R. China Geotechnical Engineering Department Nanjing Hydraulic Research Institute Nanjing 210024 P. R. China

The multi-objective optimization method was used for shape optimization of cement sand and gravel （CSG） dams in this study. The economic efficiency, the sensitivities of maximum horizontal displacement and maximum settlement of the dam to water level changes, the overall stability, and the overall strength security were taken into account during the optimization process. Three weight coefficient selection schemes were adopted to conduct shape optimization of a dam, and the case studies lead to the conclusion that both the upstream-and downstream dam slope ratios for the optimal cross-section equal 1：0.7, which is consistent with the empirically observed range of 1：0.6 to 1;0.8 for the upstream and downstream dam slope ratios of CSG dams. Therefore, the present study is of certain reference value for designing CSG dams.

关键词： CSG dam shape optimization sensitivity analysis

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Influence of modeling approaches and structural parameters on impact resistance of the human porous cranium

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Acta Mechanica Sinica 2021年第6期37卷 910-928,I0001页

作者： Qianqian wu jian Xiong Center for Composite Materials and Structures Harbin Institute of TechnologyHarbin150001China

Failure mechanism and impact resistance of a human porous cranium are studied in detail by means of theoretical and numerical *** is hypothesized that pore distribution of a cranium directly affects cranial energy absorption,and a stretched beam model and a real beam model are taken as the example for the ***,for the purpose of comparison with numerical results,a theoretical model is also proposed for the prediction of residual velocity and contact force of the impactor for an impacted *** with the real beam model,the stretched beam model containing through-thickness pores is easily deformed under the impact,thereby buffering well the external impact *** energy absorption efficiency of both the stretched beam model and real beam model is concerned with the threshold velocity for penetration which is directly related to the size of the structural damage ***,there is good agreement between numerical and theoretical *** addition,the effect of structural geometric parameters(shape and size of the impactor)on the impact resistance of the skull bone is theoretically *** study provides reference for the evaluation of the energy absorption and failure mechanism of the skull under impact loads.

关键词： Skull porous structure Impact resistance Modeling approach Theoretical prediction Influence of structural parameters

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Effect of gastric fluid on adsorption and desorption of endocrine disrupting chemicals on microplastics

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Frontiers of Environmental Science & Engineering 2022年第8期16卷 193-205页

作者： Jie wu jian Lu Jun wu CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation Yantai Institute of Coastal Zone Research(YIC)Chinese Academy of Sciences(CAS)Shandong Key Laboratory of Coastal Environmental ProcessesYICCASYantai 264003China University of Chinese Academy of Sciences Beijing 100049China Yantai Research Institute Harbin Engineering UniversityYantai 264006China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China

Microplastics and endocrine disrupting chemicals are emerging pollutants in the marine environment because of their potential *** effect of gastric fluid on the adsorption and desorption of 17β-estradiol(E2)and 17α-ethynylestradiol(EE2)to microplastics was *** adsorption and desorption isotherms of E2/EE2 on microplastics could be well fitted by the Freundlich model while the Gibbs free energy of these processes were negative,proving that the reaction occurred spontaneously on the heterogeneous surface of the *** ratios of EE2(55%–59%)on PVC were larger than that of E2(49%–55%)to indicate that EE2 was less stable in gastric fluid,which could be explained by the fact that the hydrophobicity of EE2 was greater than E2.E2/EE2 were more easily desorbed from PVC in the gastric fluid and the desorption amount(5.25–12.91/7.19–17.86μg/g)increased by 2.51 times in comparison with that in saline solution(2.22–7.81/2.87–10.80μg/g).The decrease of pH and the increase of ionic strength in gastric fluid could further strengthen desorption of E2/EE2 from *** promotion of gastric juice on desorption of PVC was achieved by reducing the hydrophobicity of the PVC *** desorption rate of E2/EE2 at 18°C and 38°C was respectively 44%–47%/46%–50%and 49%–55%/56%–59%,indicating that PVC loaded with E2/EE2 had a relatively greater risk of releasing pollutants in the gastric fluid of constant temperature marine organisms while higher temperatures exposed higher hazards for variable temperature *** interaction between microplastics and pollutants might be mainly hydrophobic interaction.

关键词： Microplastics Gastric fluid Endocrine-disrupting chemicals Adsorption Desorption

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