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Cloning One CIPK Gene from a Thermo-Sensitive Genic Self-Incompatible Line in Maize Expressing Under Different Temperatures

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Agricultural Sciences in China 2011年第6期10卷 813-819页

作者： LIN Xing-e ji Hong-qing NIU jun-hai HU Yan-min FU Zhong-jun LIU Zong-hua TANG ji-hua College of Agronomy Henan Agricultural University Zhengzhou 450002 P.R.China

A calcineurin B-like protein-interacting protein kinases （CIPK） gene was cloned （EU 424058） from a thermo-sensitive genic self-incompatibility （TSGI） line, HE97, in maize, which was selected for suppression subtractive hybridization （SSH） library of the self-incompatible and self-compatible silks of the TSGI line under different temperatures. The full-length cDNA of the candidate CIPK-like gene consisted of 1 918 nucleotides and contained a 1 332-bp open reading frame in maize. Real-time PCR indicated that the candidate CIPK-like gene was highly expressed in the self-incompatible pollen of the TGSI line, and promoted elevated levels of calcium （Ca2＋）. High Ca2＋ concentrations in the pollen strongly inhibit pollen tube formation in silk of the same plant, thus inducing self-incompatibility （SI） under high temperature. These results implied that the CIPKs-like gene would play an important role in the regulation of HE97 characteristics under different temperatures, perhaps acting as a secondary messenger in the expression of SI in the TGSI line in maize.

关键词： CIPK gene cloning expression temperature maize

Texture evolution of commercial pure Ti during cold rolling and recrystallization annealing

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International Journal of minerals,Metallurgy and Materials 2012年第6期19卷 530-535页

作者： Hai-tao jiang ji-xiong Liu Zhen-li Mi Ai-min Zhao Yan-jun Bi Metallurgical Engineering Research Institute University of Science and Technology Beijing Beijing 100083 China

X-ray diffraction （XRD） was employed to analyze the texture evolution of commercial pure （CP） Ti during cold rolling and re- crystallization annealing. The texture components were measured by electron backscattered diffraction （EBSD） after recrystallization an- nealing. The CP Ti tends to form a texture with the basal pole tilted 30°-40° away from the normal direction toward the transverse direction. The texture of the initial hot-rolled plate can be classified into three kinds, i.e., the pyramid texture （1013）[5230] and （2021）[1015], the basal plane texture （0001）[2110], and the stronger_prism_texture （1120）[0001]. After cold rolling and annealing （700℃,60 min）,_the main texture components are the cold-rolled texture （1125）[1123] and the recrystallized texture （1013）[5230]. The texture （2021）[1015] is in- herited from the texture of the initial hot-rolled plate with the decrease of orientation density gradually. The volume contents of the cold-rolled texture {2115}（0110） and the recrystallized texture {1013}（1210） are calculated by EBSD. After recrystallization annealing, the specimen is rich in the recrystallized texture and inherits some of texture components from the cold-rolled texture. When the annealing time is prolonged, the anisotropic value decreases.

关键词： titanium cold rolling recrystallization annealing textures

Virtual Reality-Based Random Dot Kinematogram

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Computers, Materials & Continua 2021年第9期68卷 4205-4213页

作者： jun Ma Hyo-jung Kim ji-Soo Kim Eek-Sung Lee min Hong Department of Software Convergence Soonchunhyang UniversityAsan31538Korea Research Administration Team Seoul National University Bundang HospitalSeongnam13620Korea Dizziness Center Clinical Neuroscience CenterDepartment of NeurologySeoul National University Bundang HospitalSeongnam13620Korea Department of Neurology Seoul National University College of MedicineSeoul03080Korea Department of Neurology College of MedicineSoonchunhyang UniversityBucheon14584Korea Department of Computer Software Engineering Soonchunhyang UniversityAsan31538Korea

This research implements a random dot kinematogram(RDK)using virtual reality(VR)and analyzes the results based on normal *** motion perception is one of visual functions localized to a specific cortical area,the human motion perception area(human analogue for the middle temporal/middle superior temporal area)located in the parieto–occipito–temporal junction of the human *** RDK measures visual motion perception *** stimuli in conventional RDK methods are presented using a monitor screen,so these devices require a spacious dark room for installation and ***,VR technology has been implemented in different medical *** test method proposed in this study include a VR-based RDK that can independently measure human motion perception abilities without any spatial constraints via a VR head-mounted ***,the VR-based RDK was implemented,and the visual perception abilities of the normal subjects were measured based on varying *** both screen-and VR-based RDK tests,the easier the stimulus is,the higher the correct answer rate and the shorter the reaction *** significant differences in coherence thresholds were observed between the two test *** VRbased RDK proposed in this study can be used as a diagnosis tool for visual motion perception and neurodegenerative disorders affecting the posterior region of the brain.

关键词： Random dot kinematogram virtual reality visual psychophysics diagnostic device

Quantum interference of a time-dependent wave packet of atom irradiated by an ultra-short laser pulse

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Chinese Physics B 2020年第1期29卷 223-228页

作者： Wen-min Yan ji-Gen Chen jun Wang Fu-ming Guo Yu-jun Yang Institute of Atomic and Molecular Physics Jilin UniversityChangchun 130012China jilin Provincial Key Laboratory of Applied Atomic and Molecular Spectroscopyjilin University Changchun 130012China Zhejiang Provincial Key Laboratory for Cutting Tools Taizhou UniversityTaizhou 318000China

The wave packet evolution of an atom irradiated by an intense laser pulse is systematically investigated by using the numerical solution of the time-dependent Schr?dinger *** are two types of spatial interference structures in the time-dependent evolution of the atomic wave *** the increasing of the evolution time,the interference fringe spacing for typeⅠ(typeⅡ)becomes larger(smaller).As the wavelength of the incident laser increases,the interference of the wave packet is changed from typeⅡto typeⅠ,and the shift of interference type can be attributed to the contribution of excited states by using the energy analysis of the time-dependent wave function.

关键词： high-order harmonic generation above-threshold ionization

Nanoceramic composites with duplex microstructure break the strength-toughness tradeoff

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Journal of Materials Science & Technology 2020年第23期58卷 1-9页

作者： ji Zou Hai-Bin Ma jing-jing Liu Wei-min Wang Guo-jun Zhang Zheng-Yi Fua State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of TechnologyWuhan430070China School of Metallurgy and Materials University of BirminghamB152TTBirminghamUK China Nuclear Power Technology Research Institute Shenzhen518026China State Key Laboratory for Modification of Chemical Fibers and Polymer Materials Institute of Functional MaterialsDonghua UniversityShanghai201620China

There is a strength and fracture toughness tradeoff in nanoceramic composite. The strength varies reciprocally with the grain size whereas the toughness contributed by compressive residual stress increases with the dimension of the second phase. In this work, a novel duplex microstructure with reinforced clusters composing of nanosized grains was proposed and validated using a model system of B_(4)C-TiB_(2) ceramics densified by carbide boronizing. As-obtained ceramics exhibit excellent combined mechanical properties at room temperature, including Vickers hardness, Young's modulus, flexural strength and fracture toughness(by surface crack in flexure method) of 32.1 ± 2.7 GPa, 506.9 ± 2.0 GPa,1175 ± 71 MPa and 5.1 ± 0.4 MPa m^(0.5), respectively. Both strength and toughness are at least ~30 % higher than the counterparts with similar composition but homogenously distributed TiB_(2) grains. Graphite onion was confirmed to be an intermediate product during reactive sintering, it facilitated the grain pullout during fracture and retained the nanometric TiB_(2) grain in the cluster, both of which also contribute the toughening and strengthening mechanisms in the B_(4)C-TiB_(2) ceramics.

关键词： Borides Microstructure In-situ reaction Mechanical properties Boron carbide Ceramics

The dynamics beamline at SSRF

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Nuclear Science and Techniques 2024年第9期35卷 56-67页

作者： Zhen Liu Li-Hua Wang Yong jiang Ya-jun Tong Hua-Chun Zhu Te ji min Chen Zheng jiang Xiang-jun Wei Shanghai Advanced Research Institute Chinese Academy of SciencesShanghai 201204China ShanghaiTech University Shanghai 201210China

The dynamics beamline(D-Line),which combines synchrotron radiation infrared spectroscopy(SR-IR)and energy-disper-sive X-ray absorption spectroscopy(ED-XAS),is the first beamline in the world to realize concurrent ED-XAS and SR-IR measurements at the same sample position on a millisecond time-resolved *** combined technique is effective for investigating rapid structural changes in atoms,electrons,and molecules in complicated disorder systems,such as those used in physics,chemistry,materials science,and extreme ***,ED-XAS and SR-IR can be used independently in the two branches of the *** ED-XAS branch is the first ED-XAS beamline in China,which uses a tapered undulator light source and can achieve approximately 2.5×10^(12)photons/s·300 eV BW@7.2 keV at the sample *** exchange-able polychromator operating in the Bragg-reflection or Laue-transmission configuration is used in different energy ranges to satisfy the requirements for beam size and energy *** focused beam size is approximately 3.5μm(H)×21.5μm(V),and the X-ray energy range is 5–25 *** one-and two-dimensional position-sensitive detectors with frame rates of up to 400 kHz enables time resolutions of tens of microseconds to be *** distinctive techniques,such as the concurrent measurement of in situ ED-XAS and infrared spectroscopy,time-resolved ED-XAS,high-pressure ED-XAS,XMCD,and pump-probe ED-XAS,can be applied to achieve different scientific goals.

关键词： ED-XAS SR-IR Time-resolved D-line SSRF

A method to Estimate Dynamic Responses of VLFS Based on Multi-Floating-Module Model Connected by Elastic Hinges

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China Ocean Engineering 2021年第5期35卷 687-699页

作者： HUANG Heng CHEN Xu-jun LIU jun-yi MIAO Yu-ji ji Song College of Field Engineering Army Engineering University of PLANanjing 210007China China Ship Scientific Research Center Wuxi 214082China

Based on the elastic foundation beam theory and the multi-floating-module hydrodynamic theory,a novel method is proposed to estimate the dynamic responses of VLFS(Very Large Floating Structure).In still water,a VLFS can be simplified as an elastic foundation beam model or a multi-floating-module model connected by elastic *** to equivalent displacement of the two models in static analysis,the problem of rotation stiffness of elastic hinges can be ***,based on the potential flow theory,the dynamic responding analysis of multi-floatingmodule model under wave loads can be computed in ANSYS-AQWA *** assembling the time domain analysis results of each module,the dynamic responses of the VLFS can be *** of the method is conducted through a series of comparison calculations,which mainly includes a continuous structure and a three-part structure connected by hinges in regular *** results of this paper method show a satisfactory agreement with the experiment and calculation data given in relative references.

关键词： Very Large Floating Structure(VLFS) dynamic responses elastic foundation beam multi-floatingmodule model elastic hinge

Upwelling current and its relationship to the enrichment of organic matter in the Lower Silurian Longmaxi Formation,northern Chongqing-western Hubei area,southern China

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Journal of Palaeogeography 2024年第4期13卷 993-1015页

作者： jun-jun Shen jia-Kai Yan Peng-Wan Wang Yu-Man Wang Lin Zhou Yu-Bing ji min Xu Hubei Cooperative Innovation Center of Unconventional Oil and Gas of Yangtze University Wuhan 430100Hubei ProvinceChina Hydrogeology and Engineering Geology Institute of Hubei Geological Bureau Jingzhou 434020Hubei ProvinceChina PetroChina Hangzhou Institute of Petroleum Geology Hangzhou 310023Zhejiang ProvinceChina PetroChina Research Institute of Petroleum Exploration and Development Bejing 100083China Exploration and Production Research Institute Jianghan Oifield CompanySINOPECWuhan 430100Hubei ProvinceChina Zhejiang Oilfield Company Integrated Center of Exploration and Development Hangzhou 310000Zhejiang ProvinceChina

Upwelling currents play a crucial role in the enrichment of organic matter,yet the mechanisms driving this process remain incompletely understood due to methodological and data resolution *** this paper,we employ a combination of biostratigraphic classification,qualitative methods,and quantitative methods to systematically analyze the sedimentological and geochemical characteristics of the Lower Silurian Longmaxi Formation in the northern Chongqing-western Hubei area,southern *** relationship between the upwelling currents and organic matter enrichment in the shale of the Longmaxi Formation is *** indicate that the upwelling currents in the study area were primarily influenced by the foreland flexure *** the Rhuddanian(flexure-sedimentation stage)to the Aeronian(flexure-migration stage),the more intense tectonic activity led to gradual opening of the barrier between the South Qinling Ocean and the Yangtze Platform,resulting in an increase in the influx of the oceanic *** upwelling currents significantly contributed to the organic matter production,albeit without substantially affecting the preservation *** the succession of the Longmaxi Formation,the organic matter content decreased gradually from the passive continental margin to the foreland flexural stagnant basin,which was mainly due to deterioration of the preservation conditions as a result of sea level fall and increased terrigenous *** the increase in the upwelling currents,they did not decisively control the organic matter ***,during the Rhuddanian to Aeronian period,the organic matter content decreased similarly from the passive continental margin to the foreland flexural stagnant basin,influenced by reduced organic matter production caused by weakening of the upwelling currents and the worsening preservation conditions caused by sea-level *** terrigenous input had a relatively minor *** results of t

关键词： Middle-Upper Yangtze Ordovician-Silurian transition Black shale Passive continental margin Foreland flexural stagnant basin Sedimentary paleoenvironment

Functional Analysis of MYB73 of Arabidopsis thaliana Against Bipolaris oryzae

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Agricultural Sciences in China 2011年第5期10卷 721-727页

作者： jiA jiao XING ji-hong DONG jin-gao HAN jian-min LIU jun-sheng Laboratory of Molecular Plant Pathology Agricultural University of Hebei Baoding 071001 P.R.China

Bipolaris oryzae is a severe rice disease,resulting in significant economic ***73 gene of Arabidopsis was isolated based on T-DNA insertion mutants whose lose-of-function mutant increased susceptibility to *** of staining for H2O2 revealed that infection of incompatible *** caused much more strong brown patches on the leaves of myb73 mutant than those on the leaves of wild *** of MYB73 gene was induced by *** increased expression was severely impaired in the myb73 *** of MYB73 was severely decreased in the npr1,jar1,eds5,and sid2 mutants,suggesting MYB73 gene participates in the jasmonate （JA） and salicylic acid （SA） signaling *** of MYB73 who encoded an R2R3 MYB transcription factor was increased by SA,JA,and ethylene （ET） *** cDNA full-length sequence of MYB73 was 960 bp and a protein with 320 amino acids was *** predicted molecular weight of MYB73 was 34.85 *** expression of MYB73 gene was induced within 24 h of the inoculation,however,the expression of PR1,PDF1.2 and NPR1 weren＇t *** *** successfully infected myb73 mutant plants,the expression of PR1,PDF1.2 and NPR1 were ***,our results suggested that MYB73 is involved in NPR1-mediated SA and JA signaling pathways.

关键词： Arabidopsis MYB73 RT-PCR Bipolaris oryzae