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Acta Pharmacologica Sinica 2003年第12期24卷 79-84页

作者： GAO Can~3, chen Li-Wei, TAO Yi-min, chen jie, XU Xue-Jun, CHI Zhi-Qiang~2 Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031 ~3Research Center for Biochemistry and Molecular Biology, Xuzhou Medical College, Xuzhou, Jiangsu 221002, China Research Center for Biochemistry and Molecular Biology Xuzhou Medical College 江苏 Xuzhou 221002 Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 上海 200031

AIM: To define the effects and signal pathways of ohmefentanyl stereoisomers [(-)-cis-(3R,4S,2’R) OMF (F9202), (+)-cis-(3R,4S,2’S) OMF (F9204), and (-)-cis-(3S,4S,2’R) OMF (F9203)] on the phosphorylation of cAMP-response element binding protein (CREB) in cultured rat hippocampal neurons. METHODS: The effects of the three OMF stereoisomers and morphine (Mor) on cAMP accumulation and CREB phosphorylation were monitored by radioimmunoassay and Western blot analysis, respectively. RESULTS: The three OMF stereoisomers and Mor could all partially inhibit forskolin-stimulated (25μmol/L, 15min) cAMP accumulation in a dose-dependent manner and this effect could be reversed by naloxone. F9202, F9204, and Mor could significantly increase CREB phosphorylation from 2.88 to 3.59 folds over control levels after 30-min exposure. This effect was reversed by naloxone, but F9203 failed to increase CREB phosphorylation. KN-62 and staurosporine significantly blocked the opioidsinduced CREB phosphorylation, while H-89 and P

关键词： ohmefentanyl stereoisomers cAMP-response element binding protein mu opioid receptor cyclic AMP hippocampal neurons phosphorylation

Increased excitatory amino acid transporter 2 levels in basolateral amygdala astrocytes mediate chronic stress–induced anxiety-like behavior

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Neural Regeneration Research 2025年第6期20卷 1721-1734页

作者： Xirong Xu Shoumin Xuan Shuai chen Dan Liu Qian Xiao jie Tu Shenzhen Key Laboratory of Neuroimmunomodulation for Neurological Diseases Shenzhen-Hong Kong Institute of Brain ScienceShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina CAS Key Laboratory of Brain Connectome and Manipulation Brain Cognition and Brain Disease Institute(BCBDI)Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina Guangdong Provincial Key Laboratory of Brain Connectome and Behavior the Brain Cognition and Brain Disease Institute(BCBDI)Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina University of Chinese of Academy of Sciences BeijingChina Faculty of Life and Health Sciences Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina

The conventional perception of astrocytes as mere supportive cells within the brain has recently been called into question by empirical evidence, which has revealed their active involvement in regulating brain function and encoding behaviors associated with ***, astrocytes in the basolateral amygdala have been found to play a role in the modulation of anxiety-like behaviors triggered by chronic stress. Nevertheless, the precise molecular mechanisms by which basolateral amygdala astrocytes regulate chronic stress–induced anxiety-like behaviors remain to be fully elucidated. In this study, we found that in a mouse model of anxiety triggered by unpredictable chronic mild stress, the expression of excitatory amino acid transporter 2 was upregulated in the basolateral amygdala. Interestingly, our findings indicate that the targeted knockdown of excitatory amino acid transporter 2 specifically within the basolateral amygdala astrocytes was able to rescue the anxiety-like behavior in mice subjected to stress. Furthermore, we found that the overexpression of excitatory amino acid transporter 2 in the basolateral amygdala, whether achieved through intracranial administration of excitatory amino acid transporter 2agonists or through injection of excitatory amino acid transporter 2-overexpressing viruses with GfaABC1D promoters, evoked anxiety-like behavior in mice. Our single-nucleus RNA sequencing analysis further confirmed that chronic stress induced an upregulation of excitatory amino acid transporter 2 specifically in astrocytes in the basolateral amygdala. Moreover, through in vivo calcium signal recordings, we found that the frequency of calcium activity in the basolateral amygdala of mice subjected to chronic stress was higher compared with normal *** knocking down the expression of excitatory amino acid transporter 2 in the basolateral amygdala, the frequency of calcium activity was not significantly increased, and anxiety-like behavior was ob

关键词： anxiety astrocytes basolateral amygdala behavior dihydrokainic acid excitatory amino acid transporter 2 fiber photometry glutamate LDN-212320 transporter

眼外伤评分与眼球结构参数在眼外伤愈后视力评估中的价值

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国际眼科杂志 2023年第2期23卷 261-266页

作者：周智露王杰夏文涛陈捷敏郝虹霞贵州医科大学法医学院贵州省贵阳市550009 司法鉴定科学研究院司法部司法鉴定重点实验室上海市法医学重点实验室上海市司法鉴定专业技术服务平台上海市200063 佳木斯大学基础医学院黑龙江省佳木斯市154007

目的:探讨眼外伤评分(OTS)、初诊视力与眼球结构参数在眼外伤愈后视力评估中的价值。方法:选取司法鉴定科学研究院2015-06/2021-06受理并出具明确鉴定意见的眼外伤者302例302眼,根据眼外伤愈后最佳矫正视力(BCVA)进行分组,Ⅰ组63例63眼,... 详细信息

目的:探讨眼外伤评分(OTS)、初诊视力与眼球结构参数在眼外伤愈后视力评估中的价值。方法:选取司法鉴定科学研究院2015-06/2021-06受理并出具明确鉴定意见的眼外伤者302例302眼,根据眼外伤愈后最佳矫正视力(BCVA)进行分组,Ⅰ组63例63眼,BCVA<3.7;Ⅱ组70例70眼3.7≤BCVA<4.5;Ⅲ组78例78眼,4.5≤BCVA<4.9;Ⅳ组91例91眼,BCVA≥4.9。另选取纳入的眼外伤者健眼77例77眼作为对照组即Ⅴ组。分析纳入研究对象的眼外伤愈后BCVA和眼球结构参数及其相关性,并使用IBM SPSS Modeler 18.0软件建立预测眼外伤愈后视力的随机森林(RF)和支持向量机(SVM)模型。结果:纳入眼外伤者初诊视力、OTS评分、眼外伤愈后角膜分级、晶状体分级、眼底分级、视神经周围神经纤维层厚度与眼外伤愈后BCVA均存在相关性(P<0.01)。除中央视网膜厚度外,各组眼球结构参数均有差异(P<0.001)。SVM模型较RF模型预测眼外伤愈后视力的准确率高,误差在0.15以内者准确率达80%以上。结论:OTS评分与眼球结构检查可为眼外伤后视觉功能障碍的法医临床鉴定提供有效信息,在鉴别伪装视觉功能障碍中具有价值。

关键词：眼损伤眼外伤评分眼球结构参数视力预测法医临床学

基于蛋白质组学方法研究痛泻要方对肝郁脾虚证胃溃疡的干预作用

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中药材 2016年第11期39卷 2598-2604页

作者：王敏刘杰民陈玲肖欢智周素芳贵阳中医学院第一附属医院消化内科贵州贵阳550001 贵州省人民医院贵州贵阳550002 贵阳中医学院贵州贵阳550002

目的:研究肝郁脾虚证胃溃疡大鼠经痛泻要方治疗后胃窦组织蛋白质组学的变化,探讨肝郁脾虚证胃溃疡发病及痛泻要方可能的作用机制。方法:建立阿司匹林诱发大鼠胃溃疡及多种不同应激方法进行情志刺激引起的大鼠肝郁脾虚证模型,通过痛泻要... 详细信息

目的:研究肝郁脾虚证胃溃疡大鼠经痛泻要方治疗后胃窦组织蛋白质组学的变化,探讨肝郁脾虚证胃溃疡发病及痛泻要方可能的作用机制。方法:建立阿司匹林诱发大鼠胃溃疡及多种不同应激方法进行情志刺激引起的大鼠肝郁脾虚证模型,通过痛泻要方动物体内给药,研究其对于大鼠一般体征、溃疡指数、差异蛋白表达的影响。采用黏膜损伤评分法研究痛泻要方对大鼠溃疡指数的影响;采用大鼠胃窦组织样本i TRAQ蛋白质组学检测方法,探寻差异蛋白表达。结果:痛泻要方能改善胃溃疡大鼠一般体征,明显减轻胃溃疡大鼠的溃疡指数并明显减轻胃溃疡大鼠的胃液酸度,差异蛋白筛选、Gene ontology分析、差异蛋白pathway分析及基因网络分析表明,痛泻要方对肝郁脾虚证胃溃疡胃窦部差异蛋白及差异基因的表达水平有明显的修复作用,痛泻要方对肝郁脾虚证大鼠有机物代谢、大分子代谢、初级代谢、细胞代谢过程、单有机体代谢过程有明显的调控作用,并对Kit受体信号通路、蛋白酶体降解、细胞因子及炎症反应、IL-5信号通路、Omega脂肪酸氧化、MAPK信号通路、mRNA加工、补体活化途径、炎症反应通路、Statin通路、IL-3信号通路、凝血通路、条纹肌收缩、TNF-α及NF-κB信号通路、EGFR1信号通路、胰岛素信号通路、补体和凝血级联反应通路、胞浆核糖体蛋白通路等炎症通路、免疫调节通路、细胞增殖及凋亡通路及分子具有广泛的调节作用。结论:痛泻要方对阿司匹林及多种不同应激方法进行情志刺激引起的肝郁脾虚证胃溃疡模型具有明显的治疗作用,肝郁脾虚证胃溃疡发病会导致胃窦组织蛋白质组学的显著变化,而痛泻要方对差异蛋白及信号通路具有显著的修复调节作用。

关键词：痛泻要方肝郁脾虚证胃溃疡胃窦蛋白组学

Gut-brain axis as a bridge in obesity and depression:Mechanistic exploration and therapeutic prospects

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World Journal of Psychiatry 2025年第1期15卷 12-20页

作者： Rui-Ying Fang Xiao-Rui Pan Xin-Xing Zeng Zheng-Zheng Li Bo-Fan chen Hai-min Zeng jie Peng The Second Clinical Medical College The Second Affiliated HospitalJiangxi Medical CollegeNanchang UniversityNanchang 330006Jiangxi ProvinceChina

A recent study by Wang et al,published in the World Journal of Psychiatry,provided preventative and therapeutic strategies for the comorbidity of obesity and *** gut-brain axis,which acts as a two-way communication system between the gastrointestinal tract and the central nervous system,plays a pivotal role in the pathogenesis of these *** suggests that metabolic byproducts,such as short-chain fatty acids,lipopolysaccharide and bile acids,which are generated by the gut microbiota,along with neurotransmitters and inflammatory mediators within the gut-brain axis,modulate the host's metabolic processes,neuronal regulation,and immune responses through diverse *** interaction between obesity and depression via the gut-brain axis involves disruptions in the gut microbiota balance,inflammatory immune responses,and alterations in the neuroendocrine *** the gut-brain axis,for example,through a ketogenic diet,the use of probiotics,and the supplementation of antioxidants,offers new remedial approaches for obesity and *** research that explores the mechanisms of the gut-brain axis is needed to provide more evidence for clinical treatment.

关键词： Gut-brain axis Obesity Depression Gut microbiota Mechanism

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桉叶精油微胶囊工艺优化及缓释性能研究

包装工程 2017年第9期38卷 107-112页

作者：任小玲岳淑丽向红陈讷敏陈洁莹陈嘉宜周敏容华南农业大学广州510642

目的研究桉叶精油微胶囊的最佳包埋工艺。方法以β-环糊精为壁材制备桉叶精油微胶囊,通过单因素和正交试验研究微胶囊包埋工艺,确定最优包埋工艺条件。结果最佳包埋工艺中包埋温度为40℃,包埋时间为1.5 h,β-环糊精质量浓度为8 g/mL。... 详细信息

目的研究桉叶精油微胶囊的最佳包埋工艺。方法以β-环糊精为壁材制备桉叶精油微胶囊,通过单因素和正交试验研究微胶囊包埋工艺,确定最优包埋工艺条件。结果最佳包埋工艺中包埋温度为40℃,包埋时间为1.5 h,β-环糊精质量浓度为8 g/mL。在该条件下制得的微胶囊包埋率为70.33%,微胶囊收率为86.27%。缓释试验结果表明,微胶囊化可以明显降低桉叶精油的挥发速率,Avrami's方程也可以对桉叶精油微胶囊释放过程进行较好拟合。结论试验达到了预期目的,微胶囊化提高了精油利用率,延长了桉叶精油的使用寿命。

关键词：桉叶精油 β-环糊精微胶囊缓释

Locally Enhanced Flow and Electric Fields Through a Tip Effect for Efficient Flow‑Electrode Capacitive Deionization

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Nano-Micro Letters 2025年第2期17卷 1-17页

作者： Ziquan Wang Xiangfeng chen Yuan Zhang jie Ma Zhiqun Lin Amor Abdelkader Maria‑Magdalena Titirici Libo Deng College of Chemistry and Environmental Engineering Shenzhen UniversityShenzhen 518060People’s Republic of China Research Center for Environmental Functional Materials College of Environmental Science and EngineeringTongji University1239 Siping RoadShanghai 200092People’s Republic of China Department of Chemical and Biomolecular Engineering National University of SingaporeSingapore 117585Singapore Department of Engineering Faculty of Science and TechnologyBournemouth UniversityTalbot CampusFern BarrowPooleEngland BH125BBUK Institut de Chimie de Nice UniversitéCôte d’AzurUMR CNRS 727228 Av.Valrose06108 NiceFrance Department of Chemical Engineering Imperial College LondonSouth Kensington CampusExhibition RdLondon SW72AZUK

Low-electrode capacitive deionization(FCDI)is an emerging desalination technology with great potential for removal and/or recycling ions from a range of ***,it still suffers from inefficient charge transfer and ion transport kinetics due to weak turbulence and low electric intensity in flow electrodes,both restricted by the current ***,a new tip-array current collector(designated as T-CC)was developed to replace the conventional planar current collectors,which intensifies both the charge transfer and ion transport *** effects of tip arrays on flow and electric fields were studied by both computational simulations and electrochemical impedance spectroscopy,which revealed the reduction of ion transport barrier,charge transport barrier and internal *** the voltage increased from 1.0 to 1.5 and 2.0 V,the T-CC-based FCDI system(T-FCDI)exhibited average salt removal rates(ASRR)of 0.18,0.50,and 0.89μmol cm^(-2) min^(-1),respectively,which are 1.82,2.65,and 2.48 folds higher than that of the conventional serpentine current collectors,and 1.48,1.67,and 1.49 folds higher than that of the planar current ***,with the solid content in flow electrodes increased from 1 to 5 wt%,the ASRR for T-FCDI increased from 0.29 to 0.50μmol cm^(-2) min^(-1),which are 1.70 and 1.67 folds higher than that of the planar current ***,a salt removal efficiency of 99.89%was achieved with T-FCDI and the charge efficiency remained above 95%after 24 h of operation,thus showing its superior long-term stability.

关键词： Flow-electrode Capacitive deionization Current collector Tip effect Desalination

NP岗位设置对促进住院医师规范化培训的探索与思考

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中国医院管理 2017年第10期37卷 61-63页

作者：练敏周玉皆陈湘玉李秀南京鼓楼医院江苏南京210008

高级执业护师是一些发达国家和地区护理队伍中的重要组成部分。通过介绍如何通过高级执业护师岗位设置与住院医师规范化培训相结合,探索高级执业护师对规范化培训的影响与作用,以期为推进高品质的住院医规范化培训提供借鉴。

关键词：高级执业护师住院医师规范化培训岗位管理

Cortico-striatal gamma oscillations are modulated by dopamine D3 receptors in dyskinetic rats

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Neural Regeneration Research 2025年第4期20卷 1164-1177页

作者： Pengfei Wang Yuewei Bi min Li Jiazhi chen Zhuyong Wang Huantao Wen ming Zhou minjie Luo Wangming Zhang Neurosurgery Center Department of Pediatric NeurosurgeryGuangdong Provincial Key Laboratory on Brain Function Repair and RegenerationZhujiang HospitalSouthern Medical UniversityGuangzhouGuangdong ProvinceChina

Long-term levodopa administration can lead to the development of levodopa-induced *** oscillations are a widely recognized hallmark of abnormal neural electrical activity in levodopa-induced ***,studies have reported increased oscillation power in cases of levodopa-induced ***,little is known about how the other electrophysiological parameters of gamma oscillations are altered in levodopa-induced ***,the role of the dopamine D3 receptor,which is implicated in levodopa-induced dyskinesia,in movement disorder-related changes in neural oscillations is *** found that the cortico-striatal functional connectivity of beta oscillations was enhanced in a model of Parkinson’s ***,levodopa application enhanced cortical gamma oscillations in cortico-striatal projections and cortical gamma aperiodic components,as well as bidirectional primary motor cortex(M1)↔dorsolateral striatum gamma *** of PD128907(a selective dopamine D3 receptor agonist)induced dyskinesia and excessive gamma oscillations with a bidirectional M1↔dorsolateral striatum ***,administration of PG01037(a selective dopamine D3 receptor antagonist)attenuated dyskinesia,suppressed gamma oscillations and cortical gamma aperiodic components,and decreased gamma causality in the M1→dorsolateral striatum *** findings suggest that the dopamine D3 receptor plays a role in dyskinesia-related oscillatory activity,and that it has potential as a therapeutic target for levodopa-induced dyskinesia.

关键词： aperiodic components dopamine D3 receptor dorsolateral striatum functional connectivity gamma oscillations levodopa-induced-dyskinesia local field potentials neuromodulation Parkinson’s disease primary motor cortex

Efficacy and safety of Tripterygium wilfordii glycosides tablets combined with Western medicine for patients with rheumatic immune diseases

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World Journal of Clinical Cases 2025年第6期13卷 14-23页

作者： Hang Shu Xiao-Yu chen jie Zhao Pin Li Zhen Sun Department of Traditional Chinese Medicine Hangzhou Special Service Recuperation Center of Chinese People's Liberation Army Air ForceNanjing 210000Jiangsu ProvinceChina Department of Convalescence Hangzhou Special Service Recuperation Center of Chinese People's Liberation Army Air ForceNanjing 210000Jiangsu ProvinceChina

BACKGROUND Rheumatic immune diseases are a group of chronic inflammatory diseases charac-terized by joint and systemic multi-organ involvement,including rheumatoid arthritis,systemic lupus erythematosus,and Sjogren’s syndrome,among *** pathogenesis of these diseases is related to the abnormal activation and regulatory imbalance of the immune *** prevalence and morbidity of rheumatic immune diseases are high,imposing a significant burden on patients'quality of life and socio-economic ***,the treatment of rheumatic immune diseases mainly relies on Western medicine,such as non-steroidal anti-inflammatory drugs,glucocorticoids,disease-modifying antirheumatic drugs,and ***,the therapeutic effects of Western medicine are not ideal,some patients poorly respond or are resistant to Western medicine,and long-term use often causes various adverse *** To systematically evaluate the efficacy and safety of Tripterygium wilfordii gly-cosides tablets combined with Western medicine in the treatment of patients with rheumatic immune *** This study conducted a meta-analysis to systematically evaluate the efficacy and safety of Tripterygium wilfordii glycosides tablets combined with Western medicine for patients with rheumatic immune *** and English databases were searched for randomized controlled trials(RCTs)on the treatment of rheumatic immune diseases with Tripterygium wilfordii glycosides tablets combined with Western *** quality of the included studies was assessed using the Cochrane risk of bias assessment ***-analysis was performed using RevMan 5.4 *** The meta-analysis included 11 RCTs involving 1026 patients with rheumatic immune *** combined treatment significantly reduced the risk of disease recurrence(relative risk=1.07,95%confidence interval:1.01-1.15,P<0.05)and showed no significant heterogeneity(I2=0%,P=0.53),indicating that Tripterygium wilfordii glycosides

关键词： Rheumatic immune diseases Tripterygium wilfordii polyglycosides tablets Western medicine treatment Systematic review Meta-analysis