检索结果-南通市图书馆

In vivo femtosecond laser nanosurgery of the cell wall enabling patch-clamp measurements on filamentous fungi

Microsystems & Nanoengineering 2024年第2期10卷 251-267页

作者： Tanja Pajić Katarina Stevanović Nataša V.Todorović Aleksandar J.Krmpot Miroslav Živić Svetlana Savić-Šević Steva M.Lević Marina Stanić dejan Pantelić Brana Jelenković mihailo d.rabasović Institute of Physiology and Biochemistry“Ivan djaja” Faculty of BiologyUniversity of BelgradeStudentski trg 1611158BelgradeSerbia Institute for Biological Research“Siniša Stanković” University of BelgradeNational Institute of the Republic of SerbiaBulevar Despota Stefana 14211000BelgradeSerbia Institute of Physics Belgrade University of BelgradeNational Institute of the Republic of SerbiaPregrevica 11811080BelgradeSerbia University of Belgrade Faculty of AgricultureNemanjina Street 611080BelgradeSerbia Institute for Multidisciplinary Research University of BelgradeKneza Višeslava 111030BelgradeSerbia

Studying the membrane physiology of filamentous fungi is key to understanding their interactions with the environment and crucial for developing new therapeutic strategies for disease-causing ***,their plasma membrane has been inaccessible for a micron-sized patch-clamp pipette for pA current recordings due to the rigid chitinous cell ***,we report the first femtosecond IR laser nanosurgery of the cell wall of the filamentous fungi,which enabled patch-clamp measurements on protoplasts released from hyphae.A reproducible and highly precise(diffraction-limited,submicron resolution)method for obtaining viable released protoplasts was *** release from the nanosurgery-generated incisions in the cell wall was achieved from different regions of the *** plasma membrane of the obtained protoplasts formed tight and high-resistance(GΩ)contacts with the recording *** entire nanosurgical procedure followed by the patch-clamp technique could be completed in less than 1 *** to previous studies using heterologously expressed channels,this technique provides the opportunity to identify new ionic currents and to study the properties of the ion channels in the protoplasts of filamentous fungi in their native environment.

关键词： patch recording laser

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Study of TiO_2 thin films on Si substrate by the photoacoustic elastic bending method

引用

Science China(Physics,Mechanics & Astronomy) 2013年第7期56卷 1285-1293页

作者： TOdOROVI dragan M rabasovi mihailo d MARKUSHEV dragan d FRANKO Mladen TANGAR Urka Lavreni Institute for Multidisciplinary Research University of Belgrade Institute of Physics University of Belgrade Laboratory for Environmental Research University of Nova Gorica

The quantitative photoacoustic investigation of different TiO2 thin films on Si substrates is presented using the elastic bending method. Photoacoustic signal amplitude and phase spectra versus modulation frequency were measured and analyzed using different sample thicknesses. Within the proposed method particular attention is given to the analysis of optical, thermal, elastic and structural sample parameters. Considerable focus is devoted to the fitting procedure of experimental results using the two-layer sample theoretical model. Characteristics of previously developed photoacoustic apparatus are discussed, attempting to search the ideal experimental conditions which can provide a good signal-to-noise ratio and sensitivity.

关键词： photoacoustic TiO2 thin films

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Fast and scalable routing protocols for data center networks

引用

digital Communications and Networks 2023年第6期9卷 1340-1350页

作者： mihailo Vesovic Aleksandra Smiljanic dusan Kostic School of Electrical Engineering University of Belgrade11120BelgradeSerbia Amazon Web Services(AWS) ZurichSwitzerland

data center networks may comprise tens or hundreds of thousands of nodes,and,naturally,suffer from frequent software and hardware failures as well as link *** are routed along the shortest paths with sufficient resources to facilitate efficient network utilization and minimize *** such dynamic networks,links frequently fail or get congested,making the recalculation of the shortest paths a computationally intensive *** routing protocols were proposed to overcome this problem by focusing on network utilization rather than ***,the design of fast shortest-path algorithms for data centers was largely neglected,though they are universal components of routing ***,parallelization techniques were mostly deployed for random network topologies,and not for regular topologies that are often found in data *** aim of this paper is to improve scalability and reduce the time required for the shortest-path calculation in data center networks by parallelization on general-purpose *** propose a novel algorithm that parallelizes edge relaxations as a faster and more scalable solution for popular data center topologies.

关键词： Routing protocols data center networks Parallel algorithms distributed algorithms Algorithm design and analysis Shortest-path problem Scalability

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Interplay between chronic hepatitis B and atherosclerosis:Innovative perspectives and theories

引用

World Journal of Gastroenterology 2022年第4期28卷 497-499页

作者： Ivan Ranković Vladimir Milivojević Aleksandra PavlovićMarković mihailo Bezmarević Clinic for Gastroenterology and Hepatology University Clinical Center of SerbiaBelgrade 11000Serbia Clinic for Gastroenterology and Hepatology University Clinical Center of SerbiaSchool of MedicineUniversity of BelgradeBelgrade 11000Serbia department of Hepatobiliary and Pancreatic Surgery Clinic for General SurgeryMilitary Medical AcademyUniversity of DefenseBelgrade 11000Serbia

Elaboration of carotid atherosclerosis in the setting of hepatitis B virus(HBV)infection should emphasize the significance of extrahepatic manifestations of the infection *** processes comprise the pathoevolution of HBV infection,rendering it a multi-systemic disease in its *** work not only exemplified atherosclerosis as an often-underestimated contributor to the severity of HBV infection but has also highlighted the bidirectional relationship between the ***,it is suggested that HBV-induced inflammation is one of the root causes of atherosclerosis,which in turn has a consequent effect on the severity of the chronic infection disease state,creating a vicious ***,we coupled prior data with the current concepts of HBV infection to postulate intriguing perspectives and theories.

关键词： Hepatitis B virus infection Carotid atherosclerosis Hepatitis B e antigen Arterial inflammation Perspectives and trends

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Reduction of peak ground velocity by nonlinear soil response–Ⅱ:excitation by a P-wave pulse

引用

Earthquake Engineering and Engineering Vibration 2021年第4期20卷 823-841页

作者： Vlado Gičev mihailo d.Trifunac Maria I.Todorovska dept.of Computer Science Univ.Goce DelčevTošo Arsov 142000ŠtipR.Macedonia dept.of Civil Eng. Univ.Southern CaliforniaLos AngelesCalifornia 90089-2531USA department of Civil Engineering Tianjin UniversityTianjin 300350China

We study the reduction of peak velocity on the ground surface of a soil valley caused by loss of wave energy by large nonlinear strains and strain localization inside the valley,for excitation by a half-sine P-wave *** study is a follow up to our previous study of out of plane response for excitation by an *** this paper,we consider the in-plane response,and assume that the soil material does not support tension,but the normal stress at a point in the soil can be compression(negative)or zero.A point in the soil with zero stress behaves as a stress-free point,it does not transmit normal stress and appears as a crack *** of this,along with the nonlinear response associated with compression and shear,the in-plane response in this study is more complex than that of the out-of-plane SH *** study the interplay of two opposing effects:(i)jump in impedance from a higher value(half-space)to a lower value(valley),which amplifies the linear motions at the free surface of the valley,and(ii)the occurrence of nonlinear zones in the valley,which reduce the motion at the valley surface.

关键词： nonlinear soil response non-linear seismic P waves energy dissipation by yielding soil strain localization

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

An aortoduodenal fistula as a complication of immunoglobulin G4-related disease

引用

World Journal of Gastroenterology 2012年第42期18卷 6164-6167页

作者： Momir Sarac Ivan Marjanovic mihailo Bezmarevic Uros Zoranovic Stanko Petrovic Miodrag Mihajlovic Clinic for Vascular and Endovascular Surgery Military Medical Academy11000 BelgradeSerbia Clinic for General Surgery Military Medical Academy11000 BelgradeSerbia Clinic for Gastroenterology Military Medical Academy11000 BelgradeSerbia Institute of Radiology Military Medical Academy11000 BelgradeSerbia

Most primary aortoduodenal fistulas occur in the presence of an aortic aneurysm,which can be part of immunoglobulin G4(IgG4)-related sclerosing *** present a case who underwent endovascular grafting of an aortoduodenal fistula associated with a high serum IgG4 level.A 56-year-old male underwent urgent endovascular reconstruction of an aortoduodenal *** patient received antibiotics and other supportive therapy,and the postoperative course was uneventful,however,elevated levels of serum IgG,IgG4 and C-reactive protein were noted,which normalized after the introduction of steroid *** computed tomography angiography showed no *** primary aortoduodenal fistula may have been associated with IgG4-related sclerosing disease as a possible complication of IgG4-related inflammatory aortic *** grafting of a primary aortoduodenal fistula is an effective and minimally invasive alternative to standard surgical repair.

关键词： Abdominal aortic aneurysm Aortoduodenal fistula Endovascular repair Immunoglobulin G4related disease

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Mating behavior as an indicator of quality of drosophila subobscura males？

引用

Insect Science 2017年第1期24卷 122-132页

作者： Marija Savic Veselinovic Sofija Pavkovic-Lucic Zorana Kurbalija Novicic mihailo Jelic Marina Stamenkovic-Radak Marko Andjelkovic Faculty of Biology University of Belgrade Belgrade Serbia Institute for Biological Research 'Sinisa Stankovic' University of Belgrade Belgrade Serbia Serbian Academy of Sciences and Arts Belgrade Serbia

According to current theoretical predictions, any deleterious mutations that reduce nonsexual fitness may have a negative influence on mating success. This means that sexual selection may remove deleterious mutations from the populations. Males of good genetic quality should be more successful in mating, compared to the males of lower genetic quality. As mating success is a condition dependent trait, large fractions of the genome may be a target of sexual selection and many behavioral traits are likely to be condition dependent. We manipulated the genetic quality of drosophila subobscura males by inducing mutations with ionizing radiation and observed the effects of the obtained heterozygous mutations on male mating behavior： courtship occurrence, courtship latency, mating occurrence, latency to mating and duration of mating. We found possible effects of mutations. Females mated more frequently with male progeny of nonirradiated males and that these males courted females faster compared to the male progeny of irradiated males. Our findings indicate a possible important role of sexual selection in purging deleterious mutations.

关键词： condition dependence deleterious mutations female preference inbred lines ionizing radiation mutational load

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

地面扭转对多层对称建筑地震反应的影响(英文)

引用

地震工程与工程振动 1990年第3期10卷 27-40页

作者： Vinay K.Gupta mihailo d.Trifunac 美国南加州大学

研究了地震动扭转分量引起的结构扭转反应并分析了其与整个结构反应的关联性。早先的研究曾经强调扭转激扰的重要性,但并未考虑结构反应的平移与扭转分量之间的相位差的效应。将固定基底的多层对称建筑理想化为简单的集中质量模型并采... 详细信息

研究了地震动扭转分量引起的结构扭转反应并分析了其与整个结构反应的关联性。早先的研究曾经强调扭转激扰的重要性,但并未考虑结构反应的平移与扭转分量之间的相位差的效应。将固定基底的多层对称建筑理想化为简单的集中质量模型并采用随机的分析方法计算了最大反应峰值的期望值。通过考察在不同地震激扰下的各种建筑物,针对选定的一些标定参数研究了扭转作用对整体反应的相关性。

关键词：扭转建筑物地震反应抗震

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

中国RJL-1力平衡加速度计的实验分析(英文)

引用

地震工程与工程振动 1991年第1期11卷 77-88页

作者： Ali Amini Owen Hata mihailo Trifunac 美国诺思里奇加州州立大学电机与计算机工程美国南加州大学土木工程系

本文给出哈尔滨国家地震局工程力学研究所制造的中国力平衡加速度计(RJL-1)的理论和实验分析。研究发现,此传感器的传递函数与美国的力平衡加速度计(FBA-1)相同。同时观察到,用单自由度粘滞阻尼体系表示感传器的反应,对于接近于和高于... 详细信息

本文给出哈尔滨国家地震局工程力学研究所制造的中国力平衡加速度计(RJL-1)的理论和实验分析。研究发现,此传感器的传递函数与美国的力平衡加速度计(FBA-1)相同。同时观察到,用单自由度粘滞阻尼体系表示感传器的反应,对于接近于和高于自振频率的频率是不适当的。

关键词：平衡加速度计加速度计 RJL-1 实验

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Interaction between Engineering Structures and Environment, Illustrated on the Examples of Bridges in Montenegro

引用

Journal of Environmental Science and Engineering(A) 2016年第2期5卷 97-101页

作者： Radenko Pejovic Rade Zivkovic Svetlana Jovanovic mihailo Ostojic Faculty of Civil Engeering University of Montenegro Podgorica 81000 Montenegro Republic department for Urban Planning and designing Podgorica 81000 Montenegro

The basic goal of this paper is to point out the importance of design shaping as well as the key parameter of the great interaction between the structure and natural environment. However, experiences confirm the fact that the most successful structures, the bridges also, have not got that attribute because of their grandiosity but per their great integration in the nature that is environment. Beside the basic function, that is the usage to man, their integrations in the environment greately enrich the ambience. The old stone bridges are mostly well integrated in the natural environment. In the period of large building, it did not take care of the parameters （the bridges of steel, concrete and prestressed concrete）, but great attention was paid to records referring to spans and complicated forms. The author thinks it is necessary to take care about integration of all building structures in the environment, especially bridges. As a good example of successful creation, simplicity of form, and especially good integration in the natural surroundings are three bridges from Montenegro, one of them is old stone bridge, others are reinforced-concrete and steel bridges.

关键词： Bridges design interaction environment.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：