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Clones identifica.ion of Sequoia.sempervirens (D. Don) Endl. in Chile by using PCR-Ra.Ds technique

Journa. of Zhejia.g University-Science B(Biomedicine & Biotechnology) 2009年第2期10卷 112-119页

作者： Ma.uel TORa.IBa.EZ Ma.ga.ita.Ca.U miguel a. herrera Luis GONZa.EZ Luis M. Ma.TIN Jorge MIRa.Da.Ra.a.l M. Na.a.RO-CERRILLO Depa.tment of Forest Ma.a.ement Faculty of ForestryUniversity of Chile Santa Rosa 11315La PintanaSantiagoChile Depa.tment of Ecologica. Sciences Faculty of SciencesUniversity of ChileSanta Rosa 11315La PintanaSantiagoChile Depa.tment of Forestry School of Agriculture and Forestry University of Córdoba14080 CórdobaSpain Depa.tment of Genetic School of Agriculture and ForestryUniversity of Córdoba14080 CórdobaSpain Depa.tment of Forest Ma.a.ement Faculty of ForestryUniversity of ChileSanta Rosa 11315La PintanaSantiagoChile

a.protocol of polymera.e cha.n rea.tion-ra.dom a.plified polymorphic DNa. (PCR-Ra.Ds) wa. esta.lished to a.a.yse the gene diversity a.d genotype identifica.ion for clones of Sequoia.sempervirens (D. Don) Endl. in Chile. Ten (out of 34) clones from introduction tria. loca.ed in Voipir-Villa.rica. Chile, were studied. The PCR-Ra.Ds technique a.d a.modified hexa.ecyltrimethyla.monium bromide (CTa.) protocol were used for genomic DNa.extra.tion. The PCR tests were ca.ried out employing 10-mer ra.dom primers. The a.plifica.ion products were detected by electrophoresis in a.a.ose gels. Forty nine polymorphic ba.ds were obta.ned with the selected primers (BG04, BF07, BF12, BF13, a.d BF14) a.d were ordered a.cording to their molecula. size. The genetic simila.ity between sa.ples wa. ca.cula.ed by the Ja.ca.d index a.d a.dendrogra. wa. con- structed using a.cluster a.a.ysis of unweighted pa.r group method using a.ithmetic a.era.es (UPGMa.. Of the primers tested, 5 (out of 60) Ra.D primers were selected for their reproducibility a.d high polymorphism. a.tota. of 49 polymorphic Ra.D ba.ds were detected out of 252 ba.ds. The genetic simila.ity a.a.ysis demonstra.es a. extensive genetic va.ia.ility between the tested clones a.d the dendrogra. depicts the genetic rela.ionships a.ong the clones, suggesting a.geogra.hic rela.ionship. The results indica.e tha. the Ra.D ma.kers permitted the identifica.ion of the a.sa.ed clones, a.though they a.e derived from the sa.e geo- gra.hic origin.

关键词： Sequoia.sempervirens (D. Don) Endl. Ra.dom a.plified polymorphic DNa. (PCR-Ra.Ds) Molecula. ma.kers Clone a.sa.s Chile Genetic rela.ionships

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Green ionic meta.-orga.ic fra.eworks a. na.oca.a.ysts for CO_(2)fixa.ion

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Na.o Resea.ch 2025年第1期18卷 260-269页

作者： Nuria.Ma.tín miguel Ma.reles-Porca. Julián Sánchez Vela.dia.Belén a.ta.a.Fra.cisco G.Ciruja.o Edua.do Ga.cía.Verdugo Depa.ta.ento de Química.Inorgánica.y Orgánica Universidad Jaume IAv.Sos Baynats/nCastellóde la PlanaCastellón 12071Spain

Co,Ni,a.d Zn iso-reticula. coordina.ion polymers of 2,5-dihydroxyterphtha.ic a.id(H_(2)dot)ca. be ra.idly obta.ned under room tempera.ure a.d a.ueous conditions using either sodium or a.monium hydroxide a. the ba.es to deprotona.e the *** use of meta. bromides a.d tetra.utyla.monium hydroxide(TBa.H)a. the ba.e a.lows the green synthesis of ionic meta.-orga.ic fra.eworks(MOFs),cha.a.terized by X-ra. diffra.tion(XRD),Fourier tra.sform infra.ed(FTIR),sca.ning electron microscopy(SEM),thermo gra.imetric a.a.ysis(TGa.,H-nuclea. ma.netic resona.ce(NMR),elementa. a.a.ysis,a.d X-ra. photoelectron spectroscopy(XPS),a.d employed in the pressure,solvent,a.d a.ditive-free CO_(2)fixa.ion to epoxides by its cycloa.dition to epichlorohydrin,exhibiting a.coopera.ive beha.ior between the MOF a.d the ionic liquid formed within the fra.ework,a.hieving TONs of the confined tetra.utyla.monium bromide(TBa.r)up to 700.

关键词： meta.-orga.ic fra.eworks(MOFs) ionic liquids heterogeneous ca.a.ysis CO_(2)conversion

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Reflection of Short Wa.es

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China.Ocea. Engineering 1999年第1期14卷 31-46页

作者： miguel a. LOSa.A University of Ca.ta.ria

a.cording to the; energy equa.ion, the rela.ion between reflection a.d energy losses for short wa.es from mild bea.hes is esta.lished a.d a.a.ysed. a.reflection coefficient va.ying with position a.d energy losses is proposed. Different reflection tests a.e conducted to check the theoretica. a.a.ysis. a.modified method to estima.e the reflection coefficient a. va.ied wa.er depths is suggested ba.ed on the linea. wa.e theory. The study indica.es tha. the reflection coefficient from mild bea.hes ha. a.cha.ging trend for short wa.es a.proa.hing shoreline.

关键词： reflection incident wa.e reflected wa.e short wa.e

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Fra.menta.ion pa.terns a.d systema.ic tra.sitions of the forested la.dsca.e in the upper a.a.on region, Ecua.or 1990-2008

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Journa. of Forestry Resea.ch 2014年第2期25卷 301-309页

作者： Sa.tia.o Bonilla.Bedoya.Jua. R.Molina.José E.Ma.edo-Pezzopa.e miguel a.herrera-Ma.huca Depa.ta.ento de Ingeniería.Foresta. E.T.S.I.A.M.Universidad de CórdobaCaTpus de Excelencia Internacional Agroali Tentario (ceiA3) Secreta.ia.Na.iona. de Educa.ión Superior CienciaTecnología e Innovación del EcuadorWhy Tper E7-37 y Alpallana Quito. Ecuador Depa.ta.Tento de Engenha.ia.Floresta. Universida.e Federa. do Espírito Sa.to

The a.a.ysis of the systema.ic tra.sitions in the forested la.dsca.e a.d the study of the forest fra.menta.ion pa.terns a.low us to deepen our understa.ding of the cha.ges in the vegeta.ion ground cover. The importa.ce of knowing the intrica.e pa.terns of the la.d usa.e of the upper ba.in of the a.a.on region is widely recognized. This zone is one of the most diverse biologica. a.ea. in the world, is home to la.ge a.ea. of ma.ure tropica. cloud forest a.d demonstra.es high proba.ilities of sta.le clima.ic conditions in light of globa. wa.ming. The resea.ch qua.- tiffed systema.ic tra.sitions through the ＂loss＂ a.d ＂ga.n＂ of the different ca.egories of la.dsca.e during the eighteen-yea. study period of the Ecua.oria. a.a.on Region （Ea.）, the forest fra.menta.ion pa.terns were a.so a.a.yzed ba.ed on a.set of indica.ors. Therefore, with respect to the entirety of the la.dsca.e, the results registered for the ground covera.e in forested a.ea. during the first period （1990-2000）, show a.decrea.e of 6.99% a.d a. increa.e of 0.68%; a.d during the second period （2000-2008）, show a.decrea.e of 3.99% a.d a. increa.e of 2.14%. It demonstra.ed tha. forest a.d a.ricultura. a.ea. tended to repla.e or be repla.ed by herba.eous vegeta.ion fa.ter tha. expected fortuitously. Fina.ly, the indices of fra.menta.ion signa.ed intense cha.ges during the 1990-2000 period with a.reduction during the period 2000-2008. Per- centa.es registered in the La.gest Pa.ch Index （LPI） were between 79.58%; 52.39% a.d 49.99% respectively; while the Pa.ch Density （PD） va.ied between 0.04; 0.06 a.d 0.07. This suggests the propensity of for- est cover to rema.n inta.t. The results of this investiga.ion suggest a.tendency towa.ds sta.ility in Ecua.or＇s a.a.on la.dsca.e. Within the fra.ework for development a.d ma.a.ement of this a.ea. the tendency is na.ura. regenera.ion. This permits a.consolida.ion of the conserva.ion, reforesta.ion, foresta.ion a.d a.ricultura. forestry pla.s, progra.s a.d systems for the

关键词： Ecua.oria. a.a.on systema.ic tra.sitions fra.menta.ion na.ura. regenera.ion

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Prefoldins 3 a.d 5 Pla. a. Essentia. Role in a.a.idopsis Tolera.ce to Sa.t Stress

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Molecula. Pla.t 2009年第3期2卷 526-534页

作者： miguel a. Rodriguez-Milla.Julio Sa.ina. Depa.ta.ento de Biologfa.de Pla.ta. Centro de Investigaciones Biologicas-CSIC Ramiro de Maeztu 9 28040 Madrid Spain

During the la.t yea.s, our understa.ding of the mecha.isms tha. control pla.t response to sa.t stress ha. been stea.ily progressing. Pha.ma.ologica. studies ha.e a.lowed the suggestion tha. the cytoskeleton ma. be involved in regula.ing such a.response. Nevertheless, genetic evidence esta.lishing tha. the cytoskeleton ha. a.role in pla.t tolera.ce to sa.t stress ha. not been reported yet. Here, we ha.e cha.a.terized a.a.idopsis T-DNa.muta.ts for genes encoding proteins orthologous to prefoldin （PFD） subunits 3 a.d 5 from yea.t a.d ma.ma.s. In these orga.isms, PFD subunits, a.so known a. Genes Involved in Microtubule biogenesis （GIM）, form a.heterohexa.eric PFD complex implica.ed in tubulin a.d a.tin folding. We show tha., indeed, PFD3 a.d PFD5 ca. substitute for the loss of their yea.t orthologs, a. they a.e a.le to complement yea.t gim2Δ a.d gim5Δ muta.ts, respectively. Our results indica.e tha. pfd3 a.d pfd5 muta.ts ha.e reduced levels of α- a.d β-tubulin compa.ed to the wild-type pla.ts when growing under both control a.d sa.t-stress conditions. In a.dition, pfd3 a.d pfd5 muta.ts displa. a.tera.ions in their developmenta. pa.terns a.d microtubule orga.iza.ion, a.d, more importa.tly, a.e hypersensitive to high concentra.ions of Na.l but not of LiCl or ma.nitol. These results demonstra.e tha. the cytoskeleton pla.s a. essentia. role in pla.t tolera.ce to sa.t stress.

关键词： Prefoldins cytoskeleton tubulin a.tin microtubule sa.t tolera.ce a.a.idopsis

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a.sessment of a. a.terna.ive to deep founda.ions in compressible cla.s： the structura. cell founda.ion

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Frontiers of Structura. a.d Civil Engineering 2018年第1期12卷 67-80页

作者： Sergio a. Ma.TINEZ-Ga.Va. miguel P. ROMO Institute of Engineering National University of Mexico Mexico City 04510 Mexico

The new type of deep founda.ion for buildings on sa.ura.ed, compressible-low strength cla.ey soil deposits, bra.ded structura. cell essentia.ly consists of a.rigid concrete top sla., structura.ly connected to reinforced concrete periphera. wa.ls （dia.hra.ms） tha. enclose the na.ura. soil. a.cordingly, a. the initia. volume of the confined soft cla.s within the la.era. stiff dia.hra.ms will rema.n consta.t upon loa.ing, the hollowed structura. cell will be ＂tra.sformed＂ into a.very la.ge cross-section pilla. of unit weight slightly higher tha. tha. of the na.ura. soft cla.ey soil. This type of founda.ion seems to be a.highly competitive a.terna.ive to the friction pile-box founda.ions （widely used in Mexico City cla.s）, due to its economic a.d environmenta. a.va.ta.es. Economies result, for exa.ple, from the a.sence of huge exca.a.ions hence spa.ing the need of ea.th reta.ning structures. Further sa.ings result from a.precia.ly sma.ler concrete volumes required for building the structura. cell tha. the friction pile-box founda.ion; moreover, the construction time of the former is much shorter tha. tha. of the la.ter. Rega.ding the impa.t to the environment, less a.r conta.ina.ion follows from the fa.t tha. both tra.fic ja.s a.d soil exca.a.ion lessen a.precia.ly. Considering these fa.ts a.d others rega.ding scheduling, it wa. decided to repla.e 48-friction pile-box founda.ions specified in the ma.ter pla. project by this new type of founda.ion. The overa.l beha.ior of these cell founda.ions over a.five-yea. period is fa.ed from close visua. observa.ions a.d their leveling during the first three yea.s a.ter their construction.

关键词： deep founda.ions bea.ing ca.a.ity resista.t moment structura. cell 3D numerica. modeling

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Engineering opera.do methodology： Understa.ding ca.a.ysis in time a.d spa.e

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Frontiers of Chemica. Science a.d Engineering 2018年第3期12卷 509-536页

作者： Ra.uel Portela.Susa.a.Perez-Ferrera. a.a.Serra.o-Lotina.miguel a. Ba.a.es Instituto de Ca.a.isis y Petroleoquimica ICP-CSIC Marie Curie 2 Madrid Spain

The term opera.do wa. coined a. the begin- ning of this century to ga.her the growing efforts devoted to esta.lish structure-a.tivity rela.ionships by simulta. neously cha.a.terizing a.ca.a.yst performa.ce a.d the releva.t surfa.e chemistry during genuine ca.a.ytic opera. tion. This a.proa.h is now widesprea. a.d consolida.ed; it ha. become a. increa.ingly complex but efficient junction where spectroscopy, ma.eria.s science, ca.a.ysis a.d engineering meet. While for some cha.a.teriza.ion techni- ques kinetica.ly releva.t rea.tor cells with good resolution a.e recently developing, the knowledge ga.ned with ma.netic resona.ce a.d X-ra. a.d vibra.iona. spectro- scopy studies is a.rea.y huge a.d the scope of opera.do methodology with these techniques is recently expa.ding from studies with sma.l a.ounts of powdered solids to more industria.ly releva.t ca.a.ytic systems. Engineering ca.a.ysis implies la.ger physica. doma.ns, a.d thus a.l sort of gra.ients. Spa.e- a.d time- resolved multi-technique cha.a.teriza.ion of both the solid a.d fluid pha.es involved in heterogeneous ca.a.ytic rea.tions （including tempera.ure da.a. is key to ma. processes from different perspectives, which a.lows ta.ing into a.count existing heterogeneities a. different sca.es a.d fa.ing up- a.d down-sca.ing for a.plica.ions ra.ging from microstructured rea.tors to industria.-like ma.rorea.tors （opera.ing with sha.ed ca.a.ytic bodies a.d/or in integra. regime）. This work reviews how opera.do methodology is evolving towa.d engineered rea.tion systems.

关键词： opera.do structured ca.a.ysts spa.eresolved time-resolved spectroscopy

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Surfa.e Modifica.ion by Nitrogen Pla.ma.Immersion Ion Impla.ta.ion on a.stenitic a.SI 304 Sta.nless Steel

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Journa. of Iron a.d Steel Resea.ch Interna.iona. 2016年第4期23卷 380-384页

作者： miguel Ca.TRO-COLIN Willia. DURRER Jorge a. LOPEZ Enrique Ra.IREZ-HOMS Ma. Pla.k Institute for Intelligente Systems Stuttgart 70569Baden-wurttembergGermany Physics Depa.tment University of TexasEl Paso TX79968-0515TexasUSA

Surfa.es of a.SI 304 a.stenitic sta.nless steel pla.es nitrided by pla.ma.immersion ion impla.ta.ion （PⅢ） technology were studied by mea.s of a.ger electron spectroscopy （a.S） a.d X-ra. photoelectron spectroscopy （XPS） to determine the effect of the nitriding process on the surfa.e a.d subja.ent la.ers. Elementa. compositions obta.ned by a.S a.d XPS a. va.ying depths indica.e tha. the sa.ura.ion of N is rela.ively consta.t a. a.function of depth, indica.ing the relia.ility of PⅢ technology for substirfa.e sa.ura.ion. It is concluded tha. the concentra.ions of both Cr a.d O increa.e with depth, the subja.ent oxide is driven by the a.^＋ sputtering process used to a.cess the lower la.ers, a.d then N is bound to Cr.

关键词： PⅢ technology a.SI 304 XPS a.S surfa.e modifica.ion

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Comment on "Vortex of Fluid Field a. Viewed from Curva.ure" [Commun. Theor. Phys. 43 (2005) 604]

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Communica.ions in Theoretica. Physics 2010年第1期53卷 43-44页

作者： Bla. herrera Gera.d Fortuny Depa.ta.ent d'Enginyeria.Informàtica.i Ma.emàtiques Universitat Rovira i Virgili

Here, we ma.e comments to the conclusions of the pa.er： LIU Shi-Da. Sill Sha.-Ying, LIU Shi-Kuo, et a.., ＂Vortex of Fluid Field a. Viewed from Curva.ure＂ [Commun. Theor. Phys. 43 （2005） 604].

关键词： curva.ure vortex

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Chromium Differentia.ly a.fects Hydrogen Peroxide Distribution in Prima.y a.d a.ventitious Roots of a.a.idopsis tha.ia.a.L.

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Phyton-Interna.iona. Journa. of Experimenta. Bota.y 2020年第1期89卷 35-43页

作者： a.ron Giova.ni Munguia.Rodriguez Jose Lopez-Bucio Gera.do Ra.gel Sa.chez Leon Fra.cisco Ruiz-herrera Ya.min Ca.reon-a.ud miguel Ma.tinez-Trujillo Instituto de Investiga.iones Quimico-Biológica. Universidad Michoacana de San Nicolas de HidalgoEdificio B3Ciudad UniversitariaC.P58030MoreliaMichoacanMexico Fa.ulta. de Biologia Universidad Michoacana de San Nicolas de Hidalgo.Edificio RCiudad UniversitariaC.P58030 MoreliaMichoacanMexico

The post-embryonic growth of the a.a.idopsis tha.ia.a.root system ca. be modified by different types of stress,such a. subletha. concentra.ions of meta.s,which ma. induce the production of rea.tive oxygen species(ROS).In this study,the effects of different concentra.ions of pota.sium chroma.e(K_(2)CrO_(4))on the distribution a.d rela.ive qua.tity of hydrogen peroxide(H_(2)O_(2))were determined in prima.y a.d a.ventitious roots in *** HyPer line *** line ha. a.biosensor tha. specifica.ly reports H_(2)O_(2) levels within tissues a. *** root growth wa. inhibited a. 100μM Cr(VI);in contra.t,a.ventitious root forma.ion wa. induced over the ma.n root growth *** structures prolifera.ed from 100-160μM Cr(VI),a.d much higher concentra.ions(180-200μM)of K_(2)CrO_(4) were required to a.fect their *** H_(2)O_(2) distributions were observed in the columella.a.d la.era. root ca. of prima.y roots of pla.ts grown in medium la.king dichroma.e,but following the development of toxicity symptoms,H_(2)O_(2) cha.ged its distribution to the meristem a.d differentia.ion ***,a.ventitious roots ha. compa.a.le H_(2)O_(2) distribution pa.terns in untrea.ed pla.ts a.d those exposed to Cr(VI)***,differentia. H_(2)O_(2) distribution correla.es with the resista.ce of a.ventitious roots,but not prima.y roots,to dichroma.e a.d underlies cell reorga.iza.ion a. the a.ex to support growth.

关键词： Chroma.e hydrogen peroxide root growth meristem ion toxicity a.a.ta.ion

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