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LicT Modulates Biofilm Formation of Streptococcus pyogenes

Journal of Biosciences and Medicines 2014年第9期2卷 1-7页

作者： Masaaki Minami Hiroshi Takase Ryoko Sakakibara Taichi Imura Hideo Morita michio Ohta Department of Bacteriology Graduate School of Medical Sciences Nagoya City University Nagoya Japan Core Laboratory Graduate School of Medical Sciences Nagoya City University Nagoya Japan Department of Clinical Investigation Daido Hospital Nagoya Japan Department of Gastroenterology Daido Hospital Nagoya Japan School of Nursing Sugiyama Jyogakuen University Nagoya Japan

Streptococcus pyogenes frequently causes purulent infections in humans. Biofilm formation is an important virulence property of S. pyogenes because of decreased susceptibility of bacteria to antibiotic treatment. Biofilm is composed of various types of matrix including glycocalyx which is an important exocellular matrix material related to bacterial sugar metabolism. A putative antiterminator protein, LicT (Spy0571), is one of the components of the glucose-independent β-gluco-side-specific phosphotransferase system (PTS). Although the PTS, a carbohydrate metabolic system, may play a role in biofilm formation, the relationship between LicT and biofilm formation has not yet been elucidated. Here, we evaluated whether LicT affected biofilm formation in modified chemically defined medium (CDMM) supplemented with glucose or β-glucoside:salicin. We created licT- and licT-complemented mutant strains from S. pyogenes 1529. Although the licT mutant strain tended to have higher growth rate than wild-typestrain in CDMM with glucose, it had a significant lower growth rate than the wild-type strain in CDMM with salicin. In addition, the licT mutant exhibited lower biofilm formation in CDMM containing salicin than the wild-type strain by 96 well plate analysis and confocal laser scanning microscopic analysis. Our results suggest that LicT plays an important role in biofilm formation of S. pyogenes.

关键词： Streptococcus pyogenes LicT Biofilm

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Analysis of Pyelonephritis-Associated Beta Hemolytic Streptococcus in Japan

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Journal of Biosciences and Medicines 2018年第12期6卷 45-52页

作者： Masaaki Minami Hideki Nishiyama Shinoba Ikegami Takuya Hattori Makoto Kawashima michio Ohta Department of Bacteriology Graduate School of Medical Sciences Nagoya City University Nagoya Japan Department of Clinical Laboratory Japanese Red Cross Nagoya Daiichi Hospital Nagoya Japan Department of Clinical Laboratory Japanese Red Cross Nagoya Daini Hospital Nagoya Japan School of Nursing Sugiyama Jyogakuen University Nagoya Japan

Pyelonephritis is an inflammation of the kidney tissue, calyces, and renal pelvis. It is commonly caused by bacterial infection that has spread up the urinary tract. Common bacteria are Escherichia coli and Enterococcus faecalis. Beta hemolytic streptococci also cause pyelonephritis. Beta hemolytic streptococcus such as Streptococcus agalactiae, and S. dysgalactiae subsp. equisimilis, when grown on blood-agar, produce complete hemolysis around each colony, usually cause severe infections including many cases of cellulitis, pneumonia, meningitis and sepsis. However the analysis of pyelonephritis causing beta hemolytic streptococcus has seldom been investigated. In this study, we tried to clarify the relationship between beta hemolytic streptococcus and pyelonephritis patient in Japan. We investigated beta hemolytic streptococcus isolated from pyelonephritis patient at two tertiary care hospitals in the central region of Japan by analyzing the background and antimicrobial susceptibility of beta hemolytic streptococcus. We recovered total sixteen beta hemolytic streptococcus in nine years. In pyelonephritis patients, beta hemolytic streptococcus was isolated from 9 S. agalactiae and 7 S. dysgalactiae subsp. equisimilis, respectively. The numbers of streptococci from female patients were more than those from male patients. Bacteria were not isolated from under 30 year patients. All S. dysgalactiae subsp. equisimilis were isolated from 60-year-age patients. About one fifth of beta hemolytic streptococcus had erythromycin and minocycline-resistant ability. Four beta hemolytic streptococci that were all S. agalactiae, had levofloxacin-resistant ability. Only one S. agalactiae had trimethoprim-sulfamethoxazole-resistant ability. Ampicillin was effective against all beta hemolytic streptococci. In our results, beta hemolytic streptococcus caused pyelonephritis in aged people and the quarter of these bacteria had some antibiotics resistance.

关键词： Beta Hemolytic Streptococcus Pyelonephritis S. agalactiae S. dysgalactiae subsp. equisimilis Antimicrobial Susceptibility

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Clinical Characteristics of Vaginal Discharge Associated Streptococcus pyogenes at General Japanese Hospital

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Journal of Biosciences and Medicines 2018年第1期6卷 9-16页

作者： Masaaki Minami Ryoko Sakakibara Mika Watanabe Hideo Morita Naoto Kanemaki michio Ohta Department of Bacteriology Graduate School of Medical Sciences Nagoya City University Nagoya Japan Department of Clinical Investigation Daido Hospital Nagoya Japan Department of Gastroenterology Daido Hospital Nagoya Japan School of Nursing Sugiyama Jyogakuen University Nagoya Japan

Streptococcus pyogenes (S. pyogenes) is popular pathogen caused various infectious disease. This study was conducted to find out the recent clinical characteristics of S. pyogenes isolates from vaginal discharge at Japanese hospital. S. pyogenes was identified by standard laboratory procedure. Antimicrobial susceptibility testing was performed by microdilution assay according to CLSI recommendation. Total 61 S. pyogenes were isolated from vaginal discharge. The major age incidence among 0 - 10 years age group was 14 (23%), among 21 - 30 years age group, 7 (11.5%), among 31 - 40 years age group, 13 (21.3%), among 51 - 60 years age group, 8 (13.1%), and among 61 - 70 years age group, 11 (18%). The numbers of ciprofloxacin and clarithromycin no-susceptible S. pyogenes from 0 - 10, 31 - 40, and 61-70 years age group were higher than those from other groups. The numbers of clindamycin and minocycline no susceptible S. pyogenes from 0 - 10, and 21 - 40 years age group were higher than those from other group. Furthermore, our study revealed that clarithromycin was completely ineffective in 21 - 30 years age group (p S. pyogenes because the trend of infectious disease epidemiology is always changing drastically.

关键词： Streptococcus pyogenes Susceptibility Antimicrobial Resistance Vaginal Discharge Clarithromycin

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利用电镀铜的技术手段计算压缩机阀片应力

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制冷技术 1985年第4期 60-68页

作者： michio Kumasawa

在压缩机运转状态下,利用电镀铜技术研究了在进气阀上的反复弯曲和撞击应力,並进行了一系列回转——弯曲疲劳试验,以确定应力级与出现斑点之间的相对关系。由于循环应力作用,在镀铜层中引起了斑纹的急剧变化。安装在全封闭压缩机上表面... 详细信息

在压缩机运转状态下,利用电镀铜技术研究了在进气阀上的反复弯曲和撞击应力,並进行了一系列回转——弯曲疲劳试验,以确定应力级与出现斑点之间的相对关系。由于循环应力作用,在镀铜层中引起了斑纹的急剧变化。安装在全封闭压缩机上表面镀有铜层的吸气阀片,在压缩机运转状态下进行试验工作。本文通过对弯曲疲劳试验结果与阀片表面出现斑点进行比较,结合阀片的支承条件,估算反复弯曲和冲击应力,并阐明反复弯曲和冲击应力的产生机理。

关键词：吸气阀片应力集中自由边缘压缩机压气机气阀电镀铜

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战后日本中国史研究的动态与特点

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江汉论坛 2009年第4期 97-100页

作者：谷川道雄日本京都大学河合文化教育研究所

第二次世界大战后,围绕着中国史研究重新体系化这一目标,日本中国史学界京都学派和历史学研究会之间,以及其他相关学派之间曾经展开了热烈的讨论。20世纪60年代后,随着日本社会的巨大变化,宏观上把握中国古代社会状况的史学研究归于沉寂... 详细信息

第二次世界大战后,围绕着中国史研究重新体系化这一目标,日本中国史学界京都学派和历史学研究会之间,以及其他相关学派之间曾经展开了热烈的讨论。20世纪60年代后,随着日本社会的巨大变化,宏观上把握中国古代社会状况的史学研究归于沉寂,日本汉学界更加注重对个别事物进行观察。虽然日本汉学界有克服微观个体研究局限性的尝试,但就学术界总体而言,还远远不够。

关键词：战后日本汉学动态特点

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Insulin resistance and chronic liver disease

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World Journal of Hepatology 2011年第5期3卷 99-107页

作者： Takumi Kawaguchi Eitaro Taniguchi Minoru Itou Masahiro Sakata Shuji Sumie michio Sata Department of Disease Information & Research Kurume University School of MedicineKurume 830-0011Japan Department of Medicine Kurume University School of MedicineKurume 830-0011Japan

Increased insulin resistance is frequently associated with chronic liver disease and is a pathophysiological feature of hepatogenous *** factors including hepatic parenchymal cell damage,portalsystemic shunting and hepatitis C virus are responsible for the development of hepatogenous insulin resistance/*** it remains unclear whether insulin secretion from pancreatic beta cells is impaired as it is in type 2 diabetes,retinopathic and cardiovascular risk is low and major causes of death in cirrhotic patients with diabetes are liver failure,hepatocellular carcinoma and gastrointestinal *** A1c is an inaccurate marker for the assessment and management of hepatogenous ***,exogenous insulin or sulfonylureas may be harmful because these agents may promote ***,pathogenesis,cause of death,assessment and therapeutic strategy for hepatogenous insulin resistance/diabetes differ from those for lifestyle-related type 2 *** this article,we review features of insulin resistance in relationship to chronic liver *** also discuss the impact of anti-diabetic agents on interferon treatment and hepatocarcinogenesis.

关键词： Viral hepatitis Hyperinsulinemia Hypoglycemic drug Hepatoma

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Clinical Characteristics of Invasive Methicillin-Resistant Staphylococcus aureus at General Hospital in the Central Region of Japan from July 2014 to June 2015

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Journal of Biosciences and Medicines 2015年第9期3卷 9-14页

作者： Masaaki Minami Ryoko Sakakibara Taichi Imura Hideo Morita Naoto Kanemaki michio Ohta Department of Bacteriology Graduate School of Medical Sciences Nagoya City University Nagoya Japan Department of Clinical Investigation Daido Hospital Nagoya Japan Department of Gastroenterology Daido Hospital Nagoya Japan School of Nursing Sugiyama Jyogakuen University Nagoya Japan

Methicillin-resistant Staphylococcus aureus is crucial pathogen caused severe invasive infection disease. This study was conducted to find out the prevalence and antimicrobial susceptibility pattern of invasive Methicillin-resistant Staphylococcus aureus isolates at general hospital in the central region of Japan from July 2014 to June 2015. Methicillin-resistant Staphylococcus aureus was identified by standard laboratory procedure. Antimicrobial susceptibility testing was performed by micro dilution assay according to CLSI recommendation. Invasive Methicillin-resistant Staphylococcus aureus disease was defined as isolation of bacteria from a normally sterile body site. One hundred seventy-one methicillin-resistant Staphylococcus aureus were isolated among which 95 (55.6%) were from inpatient and 76 (44.4%) were from outpatient. The age incidence of (0 - 1) years, (1 - 10) years, (11 - 40) years, (41 - 60) years and >60 years age groups were 18 (10.5%), 41 (24.0%), 15 (8.8%), 5 (2.9%), and 92 (53.8%) respectively. There was significant difference of age distribution between invasive and noninvasive disease in 0 - 1 years group and 11 - 40 years age group. Positive samples were received mostly from the pediatrics (56/32.7%), respiratory medicine (25/14.6%) and general medicine (25/14.6%). We also found the significant differences of department between invasive and noninvasive disease in pediatrics, dermatology, and surgery. Arbekacin, teicoplanin, and vancomycin were the most active antibiotics with 100% susceptible rates in our study. Our study revealed that erythromycin and gentamicin were more antimicrobial effective in invasive disease than in noninvasive disease significantly. Methicillin-resistant Staphylococcus aureus infection spreads worldwide easily and inadequate use of antibiotics contributes to uptake of their new antimicrobial resistance. Continuous antimicrobial surveys are need for guiding policy on the adequate use of antibiotics to reduce the morbid

关键词： Methicillin-Resistant Staphylococcus aureus Susceptibility Antimicrobial Resistance Invasive Disease

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COMT Val158Met Influences the Perception of Others＇ PainYasuyuki Nomura

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Psychology Research 2012年第3期2卷 185-195页

作者： Toshiyuki Himichi Yasunobu Okuma Masayuki Kaneko Yasuyuki Nomura jun Nomura michio Nomura Kyoto University Kyoto Japan Chiba Institute of Science Chiba Japan Yokohama College of Pharmacy Yokohama Japan Johns Hopkins University Baltimore USA

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Severe Hyperphosphatemia Resulting in Acute Renal Failure and Ischemic Encephalopathy in a Patient with Infantile Leukemia

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Case Reports in Clinical Medicine 2014年第3期3卷 129-134页

作者： Atsuko Watanabe Atushi Itano Takeshi Koga Ikuma Musha michio Shimizu Ryuhei Tanaka Department of Diagnostic Pathology International Medical Center Saitama Medical University Hidaka Japan Department of Pediatric oncology/Hematology Inter-national medical center Saitama Medical University Hidaka Japan Department of Pediatrics Kasumigaura Medical Center Tuchiura Japan Department of Pediatric oncology/Hematology Inter-national medical center Saitama Medical University Hidaka Japan Department of Pediatrics Saitama Medical University Hospital Moroyama Japan

Tumor lysis syndrome (TLS), hyperleukocytosis, and disseminated intravascular coagulation (DIC) are representative oncological emergencies that overlap mutually at the beginning of therapy for aggressive leukemia. Lately recombinant urate oxidase (rUO) enables to control uric acid level and its crystallization, the most frequent risk factor for clinical TLS;therefore, hyperphosphatemia appears to be the main risk in the rUO era. We here report an infantile leukemia patient who developed severe hyperphosphatemia, resulting in acute renal failure and ischemic encephalopathy. A 9-month-old female baby was adynamic with a bulging anterior fontanel, and was diagnosed as infantile acute lymphoblastic leukemia with a mixed lineage leukemia gene rearrangement. A laboratory examination revealed leukocytosis, bicytopenia, hyperuricemia, a prolonged prothrombin time, activated partial thromboplastin time, and elevated lactate dehydrogenase level. Soon after a reduced dose of prednisolone was administered, she developed hypoxia caused by systemic inflammatory response syndrome and heart failure. Her white blood cell count decreased sharply, leading to acute renal failure due to hyperphosphatemia, which required continuous hemodiafiltration for 48 hours. Although renal function subsequently recovered, severe ischemic encephalopathy remained. She achieved morphological remission once, however, relapsed and passed away soon after. We have to pay attention to the progression of hyperphosphatemia, hyperkakemia and DIC, although hyperuricemia was controlled using rUO. Changes in electrolyte levels must be continuously monitored, and TLS, DIC and/or hyperleukocytosis should be promptly managed especially in patients who are sensitive to therapy.

关键词： Hyperphosphatemia Tumor Lysis Syndrome Hyperleukocytosis Disseminated Intravascular Coagulation Continuous Hemodiafiltration

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Computational Analyses of Docosahexaenoic Acid (DHA, C22:6, n-3) with Alzheimer’s Disease-Causing Amyloid Peptide Aβ_1-42 Reassures Its Therapeutic Utility

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Advances in Alzheimer's Disease 2016年第2期5卷 73-86页

作者： michio Hashimoto Shahdat Hossain Kentaro Matsuzaki Abdullah Al Mamun Hiroyuki Arai Osamu Shido Department of Environmental Physiology Faculty of Medicine Shimane University Izumoshi Japan Laboratory of Alternative Medicine and Behavioral Neurosciences Department of Biochemistry and Molecular Biology Jahangirnagar University Dhaka Bangladesh Department of Geriatrics and Gerontology Division of Brain Sciences Institute of Development Aging and Cancer Tohoku University Sendai Japan

The accumulation of amyloid β peptide1-42 (Aβ1-42) masses in the brains of Alzheimer’s Disease (AD) patients is associated with neuronal loss and memory deficits. We have previously reported that oral administration of docosahexaenoic acid (DHA, C22:6, n-3) significantly decreases Aβ burden in the brains of AD model rats and that direct in vitro incubation of DHA with Aβ1-42 curbs the progression of amyloid fibrillation. In the present in silico study, we investigated whether DHA computationally binds with amyloid peptides. The NMR solution structures of Aβ1-42 were downloaded from the Protein Data Bank (PDB IDs: 1Z0Q and 2BEG). The binding of DHA to Aβ peptides was assessed by molecular docking using both a flexible and rigid docking system. Thioflavin T (ThT) was used as positive control. The chemical structures of ThT and DHA were modeled and converted to the PDB format using PRODRUG. Drug-like properties of DHA were evaluated by ADME (Absorption, Distribution, Metabolism, and Excretion). DHA was found to successfully dock with Aβ1-42. Computational analyses of the binding of DHA to Aβ1-42, as evaluated by docking studies, further corroborated the inhibitory effect of DHA on in vitro Aβ1-42 fibrillogenesis and might explain the in vivo reduction of amyloid burden observed in the brains of DHA-administered AD model rats demonstrated in our previous study. These computational data suggest the potential utility of DHA as a preventive medication in Aβ-induced neurodegenerative diseases, including AD.

关键词： Docosahexaenoic Acid Alzheimer’s Disease Amyloid Beta Peptide Molecular Docking In Silico Drug Design Protein Data Bank

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