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Complex Regulation of Prolyl-4-Hydroxylases Impacts Root Hair Expansion

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Molecular Plant 2015年第5期8卷 734-746页

作者： Silvia M. Velasauez Martiniano M. Ricardi Christian Peter Poulsen Ai Oikawa Adiphol Dilokpimol Adnan Halim Silvina Mangano Silvina Paola Denita Juarez Eliana Marzol Juan D. Salgado Salter Javier Gloazzo Dorosz Cecilia Borassi Svenning Rune Moller Rafael Buono Yukiko Ohsawa Ken Matsuoka Marisa S. Otegui Henrik V. Scheller Naomi Geshi Bent Larsen petersen Norberto D. lusem Jose M. Estevez Instituto de Fisiologfa Biologia Molecular y Neurociencias (IFIBYNE-CONICET) Facultad de Ciencias Exactas y Naturales Universidad de Buenos AiresBuenos Aires C1428EGA Argentina VKR Research Centre Department of Plant and Environmental Sciences University of Copenhagen DK-1871 Frededksberg C Denmark Joint BioEnergy Institute Feedstocks Division Lawrence Berkeley National Laboratory 5885 Hollis Street Emeryville CA 94608 USA Copenhagen Center for Glycomios Department of Cellular and Molecular Faculty of Health and Medical Sciences University of CopenhagenDK-2200 Copenhagen N Denmark Department of Botany University of Wisconsin Madison WI 53706 USA RIKEN Plant Science Center Tsurumi-ku Yokohama 230-0045 Japan Laboratory of Plant Nutrition Faculty of Agriculture Kyushu University Hakozaki 6-10-1 Higashi-ku Fukuoka 812-8581 Japan Departamento de Fisiologia Biologia Molecular y Celular Facultad de Ciencias Exactas y Naturales Universidad de Buenos Aires Buenos Aires C1428EGAArgentina These authors contributed equally to the article. Present address: Fungal Physiology CBS-KNAW Fungal Biodiversity Center Uppsalalaan 8 Utrecht 3584 CT The Netherlands Present address: The Danish Stem Cell Center The Panum Institute Blegdamsvej 3B DK-2200 Copenhagen Denmark

Root hairs are single cells that develop by tip growth, a process shared with pollen tubes, axons, and fungal hyphae. However, structural plant cell walls impose constraints to accomplish tip growth. In addition to polysaccharides, plant cell walls are composed of hydroxyproline-rich glycoproteins （HRGPs）, which include several groups of O-glycoproteins, including extensins （EXTs）. Proline hydroxylation, an early post-translational modification （PTM） of HRGPs catalyzed by prolyl 4-hydroxylases （P4Hs）, defines their subsequent O-glycosylation sites. In this work, our genetic analyses prove that P4H5, and to a lesser extent P4H2 and P4H13, are pivotal for root hair tip growth. Second, we demonstrate that P4H5 has in vitro preferred specificity for EXT substrates rather than for other HRGPs. Third, by P4H promoter and protein swapping approaches, we show that P4H2 and P4H13 have interchangeable functions but cannot replace P4H5. These three P4Hs are shown to be targeted to the secretory pathway, where P4H5 forms dimers with P4H2 and P4H13. Finally, we explore the impact of deficient proline hydroxylation on the cell wall architec- ture. Taken together, our results support a model in which correct peptidyl-proline hydroxylation on EXTs, and possibly in other HRGPs, is required for proper cell wall self-assembly and hence root hair elongation in Arabidopsis thaliana.

关键词： enzymology cell expansion cell walls protein targeting proline hydroxylation root hairs

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宇宙新视野

宇宙新视野

2006年

作者：（美）C. C. 皮特森（C. C. petersen）（美）J. C. 布兰特（J. C. Brandt）胡中为刘炎译

本书图文并茂，以直观通俗的方式对宇宙进行了探索，展现了用现今世界上著名的天文观测设备看到的宇宙，阐述了所有的天文学主题。

关键词：宇宙学

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吉西他滨治疗胰腺癌的药效相关的遗传变异

吉西他滨治疗胰腺癌的药效相关的遗传变异

全国肿瘤流行病学和肿瘤病因学学术会议

作者：李亮 Gregory Jenkins Erin E.Carlson Brooke L.Fridley William R.Bamlet Gloria M.petersen Robert R.McWilliams Liewei Wang 中国医学科学院医药生物技术研究所肿瘤室

研究背景:胰腺癌是一种以吉西他滨为一线治疗药物的迅速致命的恶性疾病。因此,为更好地发挥吉西他滨治疗胰腺癌的药效,我们进行了基因型与表型的关联研究,以筛查预测吉西他滨疗效的生物标志物。方法:我们根据之前利用正常人淋巴母细胞...

研究背景:胰腺癌是一种以吉西他滨为一线治疗药物的迅速致命的恶性疾病。因此,为更好地发挥吉西他滨治疗胰腺癌的药效,我们进行了基因型与表型的关联研究,以筛查预测吉西他滨疗效的生物标志物。方法:我们根据之前利用正常人淋巴母细胞模型系统的GWAS分析结果 ,选取与吉西他滨细胞毒性最相关的200个单核苷酸多态性（SNP）,同时获取经/或不使用吉西他滨联合治疗的400例胰腺癌患者样本,进行Cox比例风险模型分析与患者总生存期（OS）的相关性:进而通过SNP基因型与吉西他滨相互作用分析,确定与吉西他滨作用相关的SNP对患者总生存期（OS）的影响。并通过Imputation分析与OS相关SNPs的200KB上下游区域中SNP对OS的影响。此外,我们还利用其它独立的537例胰腺癌患者的DNA样本,采用TaqMan探针法验证重复候选SNPs。最后,用siRNA干扰候选基因表达水平后,在体外观察吉西他滨细胞毒性,进行实验功能验证。结果:我们利用400例患者样本发现位于染色体1,3,9,和20的4个SNP位点与经吉西他滨治疗的患者OS明显相关（P＜0.01）,以及估算分析获得的4个SNP也呈现相似的相关性。并利用537例其他样本验证得到两基因中的两个估算SNP与吉西他滨治疗后患者的OS呈相关趋势。其中XXX表达水平下降能够明显降低胰腺癌细胞对吉西他滨作用的敏感性。结论:我们筛选确定了4个位于不同区域的SNP可能与吉西他滨暴露后胰腺患者的OS相关。基因XXX可能影响患者个体对吉西他滨的药物反应的差异。这些结果可能有助于我们寻找恰当的药效预测性生物标志物以更好地发挥吉西他滨治疗胰腺癌的药效。

关键词：

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应用糖皮质激素治疗咽喉痛：临床实践指南

英国医学杂志中文版 2018年第6期21卷 346-352页

作者：卫红齐(译) 苏州大学附属第二医院耳鼻咽喉科

单剂量口服糖皮质激素在治疗急性咽喉痛中扮演着怎样的角色？依据BMJ快速推荐程序，使用GRADE框架。专家小组对糖皮质激素的使用做出了弱推荐。专家组根据2017年4月发表的一项大型随机试验所引发的相关系统性综述做出了这些推荐。该试... 详细信息

单剂量口服糖皮质激素在治疗急性咽喉痛中扮演着怎样的角色？依据BMJ快速推荐程序，使用GRADE框架。专家小组对糖皮质激素的使用做出了弱推荐。专家组根据2017年4月发表的一项大型随机试验所引发的相关系统性综述做出了这些推荐。该试验报告称，糖皮质激素提高了48小时疼痛完全缓解患者的比例。

关键词：糖皮质激素急性咽喉痛激素治疗临床实践指南应用单剂量口服系统性综述专家小组

The SUPER reporting guideline suggested for reporting of surgical technique

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Hepatobiliary Surgery and Nutrition 2023年第4期12卷 534-544,I0024-I0026页

作者： Kaiping Zhang Yanfang Ma Jinlin Wu Qianling Shi Leandro Cardoso Barchi Marco Scarci Rene Horsleben petersen Calvin S.H.Ng Steven Hochwald Ryuichi Waseda Fabio Davoli Robert Fruscio Giovanni Battista Levi Sandri Michel Gonzalez Benjamin Wei Guillaume Piessen Jianfei Shen Xianzhuo Zhang Panpan Jiao Yulong He Nuria M.Novoa Benedetta Bedetti Sebastien Gilbert Alan D.L.Sihoe Alper Toker Alfonso Fiorelli Marcelo F.Jimenez Toni Lerut Aung Y.Oo Grace S.Li Xueqin Tang Yawen Lu Hussein Elkhayat Tomaz Stupnik Tanel Laisaar Firas Abu Akar Diego Gonzalez-Rivas Zhanhao Su Bin Qiu Stephen D.Wang Yaolong Chen Shugeng Gao Editorial Office AME Publishing CompanyHong KongChina School of Chinese Medicine Hong Kong Baptist UniversityHong KongChina Department of Cardiovascular Surgery Guangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesGuangzhouChina Evidence-based Medicine Center School of Basic Medical SciencesLanzhou UniversityLanzhouChina Lanzhou University Institute of Health Data Science LanzhouChina Digestive Surgery Division Department of GastroenterologyUniversity of Sao Paulo School of MedicineSão PauloBrazil Department of Cardiothoracic Surgery Imperial College Healthcare NHS TrustLondonUK Department of Cardiothoracic Surgery University Hospital of CopenhagenRigshospitaletCopenhagenDenmark The Chinese University of Hong Kong Prince of Wales HospitalShatinNew TerritoriesHong KongChina Department of Surgical Oncology Roswell Park Cancer InstituteBuffaloNYUSA Department of General Thoracic Breast and Pediatric SurgeryFukuoka UniversityFukuokaJapan Department of Thoracic Surgery AUSL RomagnaS.Maria delle Croci Teaching HospitalRavennaItaly Clinic of Obstetrics and Gynecology University of Milan-BicoccaSan Gerardo HospitalMonzaItaly Division of General Surgery and Liver Transplantation S.Camillo HospitalRomeItaly Service of Thoracic Surgery Lausanne University Hospital(CHUV)LausanneSwitzerland Division of Cardiothoracic Surgery Department of SurgeryUniversity of Alabama BirminghamBirminghamALUSA University of Lille CNRSInsermCHU LilleUMR9020-U1277-CANTHER-Cancer Heterogeneity Plasticity and Resistance to TherapiesLilleFrance Department of Cardiothoracic Surgery Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical UniversityLinhaiChina The First School of Clinical Medicine Lanzhou UniversityLanzhouChina Thoracic Surgery Service University Hospital of SalamancaBiomedical Institute of SalamancaSalamancaSpain Department of Thoracic Surgery Helios Clinic Bonn/Rhein SiegBonnGermany Division of Thoracic Surgery Department of SurgeryThe Ottawa

Background:Existing reporting guidelines pay insufficient attention to the detail and comprehensiveness reporting of surgical *** Surgical techniqUe rePorting chEcklist and standaRds(SUPER)aims to address this gap by defining reporting standards for surgical *** SUPER guideline intends to apply to articles that encompass surgical technique in any study design,surgical discipline,and stage of surgical ***:Following the EQUATOR(Enhancing the QUAlity and Transparency Of health Research)Network approach,16 surgeons,journal editors,and methodologists reviewed existing reporting guidelines relating to surgical technique,reviewed papers from 15 top journals,and brainstormed to draft initial items for the *** initial items were revised through a three-round Delphi survey from 21 multidisciplinary Delphi panel experts from 13 countries and *** final SUPER items were formed after an online consensus meeting to resolve disagreements and a three-round wording refinement by all 16 SUPER working group members and five SUPER ***:The SUPER reporting guideline includes 22 items that are considered essential for good and informative surgical technique *** items are divided into six sections:background,rationale,and objectives(items 1 to 5);preoperative preparations and requirements(items 6 to 9);surgical technique details(items 10 to 15);postoperative considerations and tasks(items 16 to 19);summary and prospect(items 20 and 21);and other information(item 22).Conclusions:The SUPER reporting guideline has the potential to guide detailed,comprehensive,and transparent surgical technique reporting for *** may also assist journal editors,peer reviewers,systematic reviewers,and guideline developers in the evaluation of surgical technique papers and help practitioners to better understand and reproduce surgical technique.

关键词： Surgical technique surgical innovation reporting guideline reporting checklist Surgical techniqUe rePorting chEcklist and standaRds(SUPER)

血压变异性预测自发性脑出血患者的院内不良预后

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中华高血压杂志 2019年第8期27卷 707-707页

作者：刘青叶鹏 Divani AA Liu X Di Napoli M Lattanzi S Ziai W James ML Jafarli A Jafari M Saver JL Hemphill JC Vespa PM Mayer SA petersen A

越来越多的证据表明,较高的收缩压变异性(systolicblood pressure variability ,SBPV)可能与脑出血患者的不良预后相关。该研究探讨了 SB PV 与院内脑出血预后之间的关系。研究者在2个医疗系统中收集了10年的自发性脑出血患者的连续数... 详细信息

越来越多的证据表明,较高的收缩压变异性(systolicblood pressure variability ,SBPV)可能与脑出血患者的不良预后相关。该研究探讨了 SB PV 与院内脑出血预后之间的关系。研究者在2个医疗系统中收集了10年的自发性脑出血患者的连续数据。记录人口统计学、病史、实验室测试、计算机断层扫描数据、院内治疗情况以及神经和功能评估。在入院后24h内获取血压记录。

关键词：脑出血患者血压变异性不良预后自发性计算机断层扫描预测收缩压变异性人口统计学

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硅微结构中热和机械应力的模拟

电子器件 1990年第3期13卷 44-44页

作者： Farzad.(?)ourahmadi Phillipw.Barth Kurt petersen 柯导明 Novasensor 1055 Mission Court Fremont CA94539(U.S.A.)

在高级硅微结构设计中有一个不可缺少的手段全今还未得到开发,这就是用来模拟复杂多层微结构的有限元方法和数据库.本文报道了一种模拟方法和许多例子.这种方法考虑了热诱导应力和其它一些参数,这些参数精确地预言了薄膜淀积、键合和封... 详细信息

在高级硅微结构设计中有一个不可缺少的手段全今还未得到开发,这就是用来模拟复杂多层微结构的有限元方法和数据库.本文报道了一种模拟方法和许多例子.这种方法考虑了热诱导应力和其它一些参数,这些参数精确地预言了薄膜淀积、键合和封装过程.本方讨论了以下几个难题:(a)不同的绝缘体在硅膜片中产生不同的应力,这是一个很重要的问题,因为用作压力传感器的膜片随着技术的进展正变得越来越薄(5μm或更少些).(b)在硅膜片和梁上制作的金属线图案引起的复杂热应力模式.而这些模式除了用有限元计算外,任何方法分析起来都是困难的.

关键词：硅微结构热效应机械应力模拟

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脊髓刺激治疗痛性糖尿病性神经病变

中国康复 2021年第11期36卷 676-676页

作者： petersen E 王一祖(译) 潘文秀(译) 谢青(译) 不详上海交通大学医学院附属瑞金医院

痛性糖尿病性神经病变(PDN)的药物治疗通常不能完全缓解症状。由于脊髓刺激(SCS)在缓解疼痛方面显现出一定的疗效,因此本研究比较了10kHz SCS和常规治疗对PND的疗效。受试者为PDN症状超过12个月的患者,并且对加巴喷丁或普瑞巴林以及至... 详细信息

痛性糖尿病性神经病变(PDN)的药物治疗通常不能完全缓解症状。由于脊髓刺激(SCS)在缓解疼痛方面显现出一定的疗效,因此本研究比较了10kHz SCS和常规治疗对PND的疗效。受试者为PDN症状超过12个月的患者,并且对加巴喷丁或普瑞巴林以及至少一种其他类型的镇痛药耐受。患者随机接受常规药物治疗(CMM)或10kHz SCS联CMM。主要结局终点为视觉模拟量表(VAS)评定疼痛减轻50%或以上,并且三个月内神经功能缺失无加重。

关键词： CMM 加巴喷丁镇痛药普瑞巴林 PDN SCS

Occurrence of Nanobacteria-Like Particles in Renal Stones of a Southern Brazilian Population

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